JPS591243B2 - Serum cholesterol increase inhibitor isolated from defatted rice bran - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention aims to develop the use of defatted rice bran, and provides a substance that suppresses the rise in blood cholesterol using this as a raw material.

Conventionally, it has been known that foods containing many indigestible ingredients such as hectin, konjac, and mannan suppress increases in serum cholesterol, and in the literature, ingredients that are difficult or impossible to digest with human digestive enzymes are called "dietary fiber." It is clearly distinguished from the conventional "crude fiber" and is used in a broader sense of indigestible ingredients, which can inhibit the occurrence of colorectal cancer, harmful substances excreted by microorganisms, "Dietary fiber" has attracted attention for its ability to absorb toxic pigments and normalize serum cholesterol levels.
Alternatively, there is interest in grain hulls such as rice bran and bran, but according to the research of the present inventor, rice bran and defatted rice bran do indeed contain relatively large amounts of indigestible components such as cellulose, hemicellulose, and lignin. Although it is presumed that ingesting it would suppress the rise in serum cholesterol, the fact is that the effect was not substantially mentioned above.

The present inventor has discovered that when starch and protein are removed from defatted rice bran, which is obtained by defatting rice bran, the residue obtained is a substance mainly composed of cellulose, hemicellulose, and lignin, which is particularly effective in reducing serum cholesterol. We found that it is highly effective in suppressing the rise in

Next, the present invention will be explained in detail along with one example of a preferred and effective method for obtaining such an effective substance.

Commercially available defatted rice bran used as raw material (moisture 10.8%, crude protein 17.0%, crude fat 1.7%, crude fiber 9.3%,
Ash content: 11.2%, soluble nitrogen-free matter: 50%) Hemicellrone: 12.5%, cellulose: 6.8%, lignin: 3.
A total of 23.2% of the 9 sections were included.

In addition, the 70th part of hemicellulose is a pentose, and the remaining 3
Oti was hexose and uronic acid, and cellulose was composed of 90% hexose and 10% pentose.

A 0.1% glucoamylase solution (Nagao Sangyo activity: 2.89 units/my) was applied to the defatted rice bran raw material to decompose and remove the starch contained therein.

In order to remove protein and ash from the obtained residue, a solvent containing sodium lauryl sulfate and ethylenediaminetetraslicic acid as main components was dissolved in water, and sodium sulfite was further added and dissolved in the solvent, which was then extracted and removed. The resulting residue was thoroughly washed and dried to obtain the residue of the present invention, ie, the separated product.

This is hereinafter referred to as the NDF fractionation section for convenience.

The NDF fraction had a composition of 47.5% hemicellulose, 29.3% cellulose, 16.3 parts lignin, 1 part total nitrogen, and 1.4 parts ash.

The appearance was a slightly yellowish white powder.

The NDF fraction thus obtained was hydrolyzed with dilute acid to decompose the hemicellulose, and the residue was thoroughly washed and dried, resulting in 60.2% cellulose and 33% lignin.
4%, hemicellulose 5%, total nitrogen 0.8%, ash 3.
A separated product substantially free of hemicellulose with a component composition of 7% was obtained.

(Hereinafter, this will be referred to as the ADF fractionation section).

The thus obtained NDF fraction and ADF fraction were subjected to a rat feeding test as detailed below, and the results showed that the NDF fraction had a remarkable effect on suppressing the rise in serum cholesterol. However, no effect was observed in the ADF fraction.

The experimental animals were Wistar detachment rats weighing approximately 70 g, which were preliminarily fed with a standard diet for 7 days, then divided into 4 groups of 6 animals per group, administered with the following experimental diet, and kept for 10 days.

Care was taken to ensure that the average body weight of each group and the amount of weight gain during the preliminary rearing period were approximately equal.

Each rat was placed in a wire mesh cage divided into 6* rooms, kept in a breeding room at a room temperature of 22±1°C, and given free access to food and water.

The composition of the experimental feed was that commonly used in cholesterol metabolism studies.

Standard feed: Feed containing sucrose and casein as main ingredients, without cholesterol and bile acids Control feed: Feed with sucrose and casein as main ingredients, cholesterol 1 part, and bile acid 0.25% Test feed I : A test diet in which part of the carbohydrates in the control diet was replaced with NDF fraction part 5. Serum cholesterol and liver cholesterol were measured using analytical methods after decapitation, blood sampling, and laparotomy, and the results shown in Table 1 below were obtained.

As is clear from the table above, the serum cholesterol level is
The DF feed group had a decrease in λ compared to the control feed group, and the ND
A remarkable effect was observed in the F fraction.

This experimental data was tested for significance using stochastic processing, and as a result, it was found that there was a significant decrease with a small risk rate of 5%.

The NDF feed group also had lower liver cholesterol levels than the control feed group, demonstrating the effect of the NDF fraction.

On the other hand, serum cholesterol was found to be lower in the ADF feed group than in the control feed group, but liver cholesterol was on the contrary increased, indicating that the ADF fraction was not preferable.

As described above, according to the present invention, the separated product made from defatted bran as a raw material and whose main components are hemicellulose, cellulose, and lignin is extremely effective in suppressing the rise in serum cholesterol.

Next, a preferred fractionation method for obtaining the NDF fraction of the present invention will be explained.

Using commercially available defatted rice bran as a raw material, sieve it through a 60-mesh sieve, and add 100 g of the passed portion to 0.1% glucoamylase solution 1
The mixture was suspended in water and reacted at 40°C for 24 hours.

Then, it was centrifuged at 3000 rpm for 5 minutes, the supernatant was removed, and the residue was mixed with 1 t of a solvent containing sodium lauryl sulfate and ethylenediaminetetrasuccinic acid as main components (30 g of sodium lauryl sulfate, 19 g of ethylenediamine tetrasuccinic acid,
7 g of sodium tetraboronate, 5 g of dibasic sodium phosphate, 10 ml of ethylene glycol monoethyl ether were dissolved in water to make 1 t, 50 g of sodium sulfite, 200 rIL of decalin were added, and the mixture was boiled with a reflux condenser.

After boiling, the mixture was centrifuged for 11.5 minutes at 3,000 rl, the supernatant was removed, and the residue was washed with 1 t of hot water and dried to obtain 25 g of NDF fraction.

The composition of the NDF fraction is hemicellulose 47% on a dry basis.
．． 5%, cellulose 29.3%, lignin 16.3%,
The composition had a total nitrogen content of 1.0% and an ash content of 1.4%.

Incidentally, the removal of ash is optional, and therefore the addition of sodium sulfite to the solvent can be omitted, and the addition of decalin acts as an antifoaming agent and facilitates the work.

The ADF fractionation sample was prepared using the 1N obtained above.
The DF fraction sample is further treated with a dilute acid aqueous solution containing ethyltrimethylammonium bromide and an antifoaming agent, and the residue is thoroughly washed.

Claims (1)

[Claims] 1. A serum cholesterol increase-inhibiting substance whose main components are hemicellulose, cellulose, and lignin obtained by using defatted rice bran as a raw material, decomposing and removing starch with amylase, and then dissolving and removing proteins with a solvent.