JPS59214762A - Liquid chromatography apparatus - Google Patents

Abstract

PURPOSE:To measure many components simultaneously with high accuracy by controlling the quantity and types of reactive reagents in accordance with the components eluted from a separating column tube. CONSTITUTION:A sample from a sampling valve 3 reaches a column 4 with an unsoluble solution 1. An eluted solution and separated components of the sample are mixed with reactive reagents by a mixer 9 and introduced into a detector 12 through a reaction bath 11. The supplying time range, the quantity to be supplied and the types to be supplied of the reactive reagents 5, 5', 5'' are controlled for each separated and eluted component by opening and closing valves 7, 7', 7'' by a program controller 6. Significant time reduction and operation cost savings are achieved and the precious samples such as blood and very fine specimens can be analyzed with the minimum consumption.

Description

[Detailed Description of the Invention] [Field of Application of the Invention] The present invention uses a gradient @ structure as a bonder for a reaction solution,
The present invention relates to a liquid chromatography device suitable for adding an optimal reaction solution to an eluted component and enabling high sensitivity or measurement of a wide variety of components.

[Background of the invention]

Conventional reaction analysis methods have a major drawback in that the flow rate and concentration of the reaction solution or the type of reaction solution are fixed, which limits the optimal reaction conditions and the components to be measured.

[Purpose of the invention]

The purpose of the present invention is to simultaneously measure components with high sensitivity by arbitrarily changing the amount of reaction solution depending on the component, or by changing to a different reaction solution in a solution in which the components have been eluted. ,
The aim is to provide a liquid chromatography device that enables simultaneous measurements of analyzes that were conventionally performed separately.

[Summary of the invention]

The apparatus of the present invention is a reaction liquid chromatograph apparatus, in which the pump flow rate and concentration on the reaction liquid side can be arbitrarily varied, and furthermore, different reaction liquids can be separated depending on the components eluted from the separation column tube, or several types of different types can be generated. By mixing all of the reaction solutions in any ratio and adjusting the reaction conditions for each component (Q4), it is possible to arbitrarily and easily set the reaction conditions, making it possible to simultaneously measure multiple components with high sensitivity. ].

[Embodiments of the invention]

The conventional channel thread and the channel system of the present invention will be explained below based on 1.

FIG. 3 shows a reaction chromatographic analysis according to the conventional method, which is poured out and reaches the sampling valve 3. By fully switching the sample link valve 3 to which all the sample has been added in advance, the sample reaches the column 4 together with the eluent and is adsorbed onto the upper bed of the column packing material. The eluate that has passed through column 4 and the separated sample components reach mixer 9 . If the eluate is in this state, the detection sensitivity is low or it cannot be detected at all, so it becomes necessary to color the eluate or convert it into a fluorescent substance. For this purpose, reaction reagents are required. The reaction reagent 5, which is limited to a certain extent, is sucked and discharged by a pump (B18) on the flow side, and reaches the mixer 9, where it is mixed with the eluate mixed with the sample.The eluate mixed with the reaction reagent is The reaction reaches the reaction tank 11, which is heated by the temperature controller 1°, and the reaction is accelerated, and the detector 12 absorbs the light slowly, or converts the fluorescence intensity into a minute voltage using a photocell or phototube, and records it in No. 113. Record.Detector 1
The reaction chromatography analysis method is carried out in the step of discarding the eluate measured in step 2 as waste liquid. In the case of these conventional methods,
Since the flow rate and reaction reagents are fixed during one analysis, each component may not be measured under optimal conditions, or some components may be impossible to measure.

On the other hand, in the case of the method shown in Fig. 2, the flow from eluent 1 to mixer 9 is the same as in the conventional method, but the selection of the three reaction reagents S, 5/, and 5// is separated and eluted. For each component, the program controller 6 sets the valve 7.7/
By opening and closing , 7//, any 1' and type can be controlled at any time. These characteristics are such that even if the components are the same, the sensitivity is markedly different when the reaction reagent is saturated or at a very low temperature.The reaction state differs depending on each component, so the method of the present invention makes it possible to effectively measure all components. .

〔Effect of the invention〕

According to the present invention, it is possible not only to measure the pheasant with high sensitivity by identifying the types of anti-inflammatory drugs, but also to achieve the purpose with only a few analyzes using conventional fixed reaction reagents. However,
As in the method of the present invention, reaction reagents are controlled by a program controller, and a variety of variable types can be selected, or multiple reaction reagents can be mixed at an arbitrary mixing ratio of 4 to perform a single analysis. For example, achieving the objective is t5J capability, which has the effect of significantly shortening the length of 1tJJ and reducing operating costs, or enabling analysis of valuable samples such as blood and minute samples with minimal consumption.

[Brief explanation of drawings]

FIG. 1 is a flow path diagram of a conventional reaction chromatograph apparatus, and FIG. 2 is a flow path diagram of a reaction chromatography apparatus according to an embodiment of the present invention.

Claims (1)

[Claims] 1. A liquid chromatography device characterized by a chromatography method in which gold is added as a reaction liquid to the eluent for measurement, and the concentration of the reaction liquid can be arbitrarily changed.