JPS5946208B2 - Livestock drugs - Google Patents

Description

[Detailed description of the invention] FIELD OF THE INVENTION The present invention relates to pharmaceuticals for livestock.

Conventionally, lactic acid bacteria preparations have been sold for the purpose of promoting the growth of livestock or preventing and treating diarrhea, and there have been cases where lactic acid bacteria have been administered to livestock for similar purposes, but the desired effects have not always been achieved.

In view of this situation, the present inventors conducted intensive research and found that when two types of bacteria, Lactobacillus acidophilus and Streptococcus faecalis, were administered to livestock, they did not compete with each other and both bacteria entered the gastrointestinal tract of livestock. It colonizes well, and the combination of the above two types of bacteria selectively has an extremely significant growth promoting effect on livestock, and a preventive and therapeutic effect on diarrhea, constipation, and sacroiliac diseases, compared to conventional lactic acid bacteria preparations and lactic acid bacteria administration examples. The present invention was completed after discovering that the effect of improving feed conversion ratio and deodorizing feces can be obtained.

That is, the present invention is a drug for livestock containing Lactobacillus acidophilus and Streptococcus faecalis as active ingredients.

Domestic animals in the present invention include artiodactyla (e.g., pigs, boars, cows, etc.), perissodactyla (e.g., horses), and carnivores (e.g., dogs, cats, etc.).
Among them, monogastric animals in the order Artiodactyla, such as pigs, are preferred.

The Lactobacillus acidophilus used in the present invention may be any species as long as it colonizes the digestive tract of the intended livestock.

Such colonization can be determined, for example, by administering a certain number of Lactobacillus acidophilus to intended livestock for a certain period of time, and determining the number of inoculated bacteria detected in feces or the period during which inoculation is observed in feces. For example, when administering 10x Yuyu bacteria to intended livestock for 5 days, inoculation of 105/9 or more is detected in the feces, or inoculation is detected for at least 5 days, especially for at least 7 days, even after administration is stopped. It is preferable that the

Among such bacteria, a preferable example is Lactobacillus acidophilus K-13 (National Institute of Microbial Technology, Agency of Industrial Science and Technology, Microbial Accession Number FE RM-P/1).
6.1955).

Furthermore, the Streptococcus faecalis used in the present invention may be any species as long as it colonizes the digestive tract of the intended livestock.

The fixability is preferably the same as that of Lactobacillus acidophilus described above.

Among these bacteria, Streptococcus is preferred.
Faecalis 129 BIO3B (National Institute of Microbial Technology, Agency of Industrial Science and Technology, Microbial Accession Number FERM-P /1
62338).

Lactobacillus acidophilus and Streptococcus faecalis may be cultured separately, or
These two types of bacteria may be mixedly cultured in one culture medium.

For culturing Lactobacillus acidophilus or Streptococcus faecalis alone or in a mixed culture of these bacteria, either a solid medium or a liquid medium can be used.

As the culture method, any of the static culture method, shaking culture method, continuous culture method, deep culture method, etc. can be used, but the static culture method or the continuous culture method in a liquid medium is particularly preferable.

As for the medium composition, one containing a carbon source, nitrogen source, etc. that can be assimilated by the bacteria is used.

As the carbon source, for example, assimilable sugars such as glucose, sucrose, and maltose are used, and as the nitrogen source, for example, peptone, corn stew liquor, meat extract, and other organic nitrogen-containing substances are used.

Small amounts of other inorganic salts, such as common salt, phosphates, metal salts such as calcium, manganese, and iron, micronutrients such as various vitamins, etc. may be added as appropriate. A surfactant such as Span may also be added.

The culture temperature is preferably kept at 20 to 45°C, especially around 37°C, and the liquid quality of the medium is suitable for culturing Lactobacillus acidophilus at pH 4.0 to 8.5, especially pH 6.5 to 6.
．． 8 is also suitable for culturing Streptomyces faecalis at pH 4.0 to 10.0, especially pH 6.5 to 8.5.
For the mixed culture of both types, a pH of 4.0 to 8.5, particularly a pH of 6.5 to 7.5 is preferable.

For mixed culture, the ratio of the number of bacteria in both fractions to be inoculated and the culture time can be adjusted as appropriate. By setting the ratio of the number of inoculated bacteria to approximately 100:1 and culturing for 17 hours, the ratio of the number of bacteria in both plots at the time of bacterial collection can be approximately 1:1.

Lactobacillus acidophilus and Streptococcus faecalis used in the present invention may be in any state as long as they are viable, such as cultured bacteria, vacuum-dried products thereof, freeze-dried products thereof, and spray-dried products thereof. can give.

Lactobacillus acidophilus and Streptococcus faecalis, which are the active ingredients of the present invention, may be administered directly to livestock, or they may be formulated into powders, tablets, granules, pellets, etc. by known lactic acid bacteria preparation manufacturing means and administered. It may also be administered by adding it to feed, drinking water, etc.

The number of Lactobacillus acidophilus and Streptococcus faecalis in a preparation is appropriately determined depending on the dosage form, type of strain, target livestock type, symptoms, etc., but usually the number of bacteria in both groups is 10 per gram of the preparation. '〜
1011, especially 106 to 1010 are preferred.

Further, the mixing ratio of the two parts in the preparation may be any ratio as long as both parts contain the above-mentioned number of bacteria per gram of the preparation.

The number of Lactobacillus acidophilus and Streptococcus faecalis to be administered to livestock is appropriately determined depending on the type of livestock, type of disease, symptoms, etc.;
Advantageously, 105 to 10'/kg of each of S. acidophilus and S. faecalis are administered per day.

If desired, Lactobacillus acidophilus and Streptococcus faecalis may be administered to livestock simultaneously as separate formulations.

Actual example 1 Streptococcus faecalis 129 B103B
and Lactobacillus acidophilus K13 in a bouillon culture medium supplemented with 1% glucose; Twin 8
00.1% supplemented bouillon culture medium at 37°G, respectively.
After culturing for 20 hours, the bacteria were collected using a centrifuge, and each bacterial cell was added to an aqueous solution of 1% lysine, 0.05% cystine, and 0.05% ascorbic acid, and about 100 microorganisms were added to each dish.
Adjust the bacterial solution so that the number of bacteria is 0 billion.

Next, mix 1107 of each type of bacteria, add 80 kg of sterilized starch and mix well to make it homogeneous, and the water content is 11~
Prepare 14% bacterial starch.

This bacterial starch is placed in a shallow container, placed in a multistage vacuum dryer, and dried at a vacuum of 1 mmHg for about 5 hours.

The thus obtained dried bacterial starch 1%, potato starch 80%, calcium carbonate 5%, bentonite 10%
, a powder consisting of 4% calcium carbonate.

Actual Example 2 1% dried bacterial starch obtained in Actual Example 1, 11% lactose, 75% potato starch, 10% tljn, CMC3
Granules made by adding water to a mixture of % and kneading it into granules.

Example 3 Lactobacillus acidophilus K13 and Streptococcus faecalis 129 BIO3B, which were cultured and collected under the same conditions as in Example 1, were mixed with a dispersion medium (0.75% gelatin, 0.1 M sodium glutamate, 0 sodium succinate). Hedextrin in a solution consisting of .1M
0%) and mix well to prepare a bacterial suspension so that the number of each bacteria is about 100 billion in 1 rfLl. A Mobil Miner type spray dryer (manufactured by 0) has a rotation speed of 25,000 rpm, an inlet temperature of 120°C, and an outlet temperature of 6.
Spray drying was carried out under the conditions of 0°C and a suspension dropping rate of 2000 ml/hr.

The thus obtained dried fungal bed contains 0.1%, lactose 30%, calcium carbonate 5%, potato starch 60%, and talc 4%.
．． Experimental Example of Tablets Compressed from 9% Powder (Experimental Method) The following seven types of lactic acid bacteria preparations and lactic acid bacteria-free preparations were prepared.

(1) Lactobacillus acidophilus K13 and Streptococcus faecalis 129 B103B each contain approximately the same number of bacteria, and the total number of bacteria is 10.
Dry starch powder (LA+
(abbreviated as SF).

(2) Dry starch powder adjusted to have a Lactobacillus acidophilus K13 bacterial count of 108/g (
(abbreviated as LA).

(3) Streptococcus faecalis 129B
Dry starch powder (abbreviated as SF) adjusted to have an IO3B bacterial count of 108/g.

(4) Bifidobacterium bifidrum G9-
1 (National Institute of Microbial Technology, Agency of Industrial Science and Technology, Microorganism Accession Number F
Dry starch powder (abbreviated as BB) adjusted to have a bacterial count of 108/g (ERM-P A1594).

(5) Lactobacillus acidophilus K13c! :
Each contains approximately the same number of bacteria as Bifidobacterium bifidrum G9-1, and the total number of bacteria is 108/
,! Dry starch powder (L
(abbreviated as A+BB).

(6) Streptococcus faecalis 129BIO
3B and Bifidobacterium beefidrum G9-1
Each contains approximately the same number of bacteria, and the total number of bacteria is 108/! Dry starch powder adjusted to be li' (abbreviated as SF+BB).

(7) Dry starch powder (abbreviated as additive-free) was prepared from 3 to 4 litters (from landraces) that were born at about the same time, after weaning at 30 days of age, the piglets were divided into 4 sections, with 6 piglets per section. Animals are housed in groups so that genetic factors and average body weight are as similar as possible.

In the growth promotion test and diarrhea prevention test, each formulation prepared as above was added to artificial milk B* at 0.2%.
The animals were fed ad libitum and observed for 5 weeks in the growth promotion test and 2 weeks in the diarrhea prevention test.

In the diarrhea treatment test, piglets with watery diarrhea that sporadically occurred in a pigpen were selected, and 10 piglets were selected in each group, and 1 g/day of each of the above preparations was administered orally by force for observation. .

*Prescription of artificial milk B Yellow corn 20% barley
19% wheat flour 14% soybean flour
4% protoflower■
Nippon Koyu ■] 5% Ribbonku■ [Kuraide Scientific Feed]
5% skimmed milk powder 9
．． 65% powdered oil 1.5%
Glucose 5% yeast
5% bran 6.8% rice bran
4% trilime
0.7% vitamin/mineral mixture
0.2% togen [F] [Ueno Pharmaceutical ■]
0.15% (result)

Claims (1)

[Claims] 1. A drug for livestock containing Lactobacillus acidophilus and Streptococcus faecalis.