JPS5946564B2 - Nori seedling method - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention promotes sporangial formation by irradiating red light with a wavelength of 600 to 700 mμ to glue filaments, and obtains a large amount of mature sporicidal spores from the sporangial cells in a short period of time. It is related to the characteristic method of collecting seedlings using glue.

Cultivation of filaments to obtain sporicide in seaweed aquaculture is an important task that determines the success or failure of seaweed aquaculture production.

Sporicides for seeds and seedlings in conventional seaweed cultivation are cultured from spring to early autumn using shells of oysters, scallops, abalone 5, etc. as so-called shell filaments.

Generally, seedlings are collected from these shell filaments by attaching sporicidal spores, which are released under natural conditions of low temperatures and short days in early autumn, to a glue net. It is difficult to precisely control the timing.

Furthermore, culturing of shell filaments requires a fairly large space and facilities, and there are drawbacks such as the fact that it requires a lot of effort to manage.

The present inventors investigated to eliminate these drawbacks and invented a unique method for collecting seedlings using glue.

In other words, a light source having a certain wavelength (in the present invention, it has been found that the use of red light is most effective).

) is irradiated to promote sporangial formation, a large amount of sporicidal spores are obtained from the sporangial sacs in a short period of time, and the leaf bodies are propagated from the sporicidal spores.

According to the research conducted by the present inventors, when the glue filaments are irradiated with a certain wavelength range, for example, pure red fluorescent lamp or pure blue fluorescent lamp, the formation of sporangia is specifically promoted. We have completed the present invention by discovering that a large amount of sporicide can be obtained in a short period of time, that pure red fluorescent lamps are particularly effective, and that this method can be used in actual seaweed cultivation.

The glue filament used in the present invention is
Free living filaments or shell filaments may be used. If free living filaments are used, the filament colonies (diameter of 1 or more) may be used as is, or the filament colonies may be cut with a mixer etc. .

When using a free-living filament in the present invention, place the filament in a container with seawater, cover with a transparent glass plate, acrylic plate, vinyl chloride plate, etc., and place the filament at a distance of 30 cIrL from the specified light source to the upper lid. Place the container and irradiate it with a prescribed light source.

In addition, when using shell filaments, the culture side of the shell is turned upward, and a predetermined light source is irradiated from 30 cIrL above.

Next, the present invention will be explained in detail with reference to Examples.

Example 1 Porphyra species (scientific name: Porphyra
yezoensis ), Malva asafsa species (scientific name: P
Porphyra kuniedai) and Porphyra tenera species (Scientific name: Porphyra tenera)
Free-living filamentous colonies of Var, ) (
811g1) in diameter, and put 3 pieces of this into each transparent glass petri dish with a diameter of 9crIL, 5 pieces each.
Dispense it with 0 ml of culture seawater, place the Petri dish with the lid on at 22°C ± 1°C, and place it at a distance of 30 crrL from the specified light source to the top lid of the Petri dish. After continuous irradiation for 10 days with pure red fluorescent light, pure blue fluorescent light, Plantlux, and daylight color fluorescent light (20 W each) for 10 days, one colony was placed in an 11-volume lower flask with seawater for culture. 5cIfL long Cremona thread was added once a day as a substrate for spore settlement, and the seedlings were collected once a day during the dark period for 3 days with aeration and agitation.

Cremona thread after seedling collection is used to count the number of epiphytic germinated spores.
Aerate and stir the culture seawater in a square 41-capacity aquarium made of transparent acrylic resin, and mix CO2 gas into the aerated air during the light period to adjust the seawater to a range of 7.9 to 8.5. , Illuminance of the aquarium light-receiving surface with metal halide lamps for 12 hours a day: 2
Cultivation was carried out under lighting conditions such that the temperature was 0 kg, and the water temperature was maintained at 17°C.

Ten days after the start of culture, the lobules that had grown and adhered to the Cremona threads were counted using a microscope.

The results are shown in Table 1. As shown in Table 1, it can be seen that the area irradiated with pure red fluorescent light specifically releases a large amount of spores.

Example 2 Susabi species (scientific name: Porphy) produced by a known method
ra yezoensis), Porphyra kuniedai (scientific name: Porphyra kuniedai) and Porphyra tene (scientific name: Porphyra tene)
ra Var,) free-living filamentous colonies (
Approximately 7 m in diameter) using a mixer, 10,000 r.o.
pam.

Cutting in 1 minute yields threads of 30-300μ.

Example 1 An amount equivalent to 0.1 filament was dispensed into a glass petri dish with a diameter of 9 cIIL along with 30 ml of seawater.
The cells were cultured in the same manner as above, and the results shown in Table 2 were obtained.

As shown in Table 2, it can be seen that a large amount of spores are specifically released in the area irradiated with pure red fluorescent lamp.

Example 3 Susabi species (scientific name: Porphy) produced by a known method
ra yezoensis), Porphyra kuniedai (scientific name: Porphyra kuniedai) and Porphyra ten (scientific name: Porphyra ten)
era Var, ) shell filaments were punched out with a 10M diameter corkpolar, placed in a glass petri dish with a diameter of 9 cIrL, 5 shells at a time, with the culture side facing up, along with 30 ml of seawater, and placed with a specified light source from above the 30 cIrL. 5
Irradiated for days.

At this time, a pure red fluorescent lamp manufactured by Matsushita Electric Industrial Co., Ltd. was used as a light source, and a pure blue fluorescent lamp, a plant look 4-light lamp, and a daylight color fluorescent lamp were each used at 20 W each.

In addition, as a control, we cultured at 17°C for 7 days at 5,000 lux, a light cycle of 8 hours of light and 16 hours of darkness, which is a so-called low-temperature treatment. A short-day treatment area was established.

After the treatment, each treated shell was placed in an 11-volume lower flask with seawater under conditions of 8 hours of light and a temperature of 17°C.
Using 5cIrL long Cremona thread as a substrate, the seedlings were collected during the dark period for 3 days with aeration and stirring.

In order to count the number of spores that have settled and germinated, the Cremona threads after seedling collection were aerated and stirred in culture seawater in a square 41-capacity aquarium made of transparent acrylic resin, and during the light period, 002 gas was mixed in the aerated air. Seawater (7) While adjusting the pH to a range of 7.9 to 8.5, illuminate the aquarium with a metal halide lamp for 12 hours a day so that the illumination intensity on the light receiving surface of the tank is 20 kilolux, and the water temperature is 17°C.
Culture was carried out while maintaining the temperature.

Ten days after the start of culture, the lobules that had grown attached to the Cremona threads were counted using a microscope.

The results are shown in Table 3.

As shown in Table 3, it can be seen that the area irradiated with pure red fluorescent light specifically releases a large amount of spores.

Claims (1)

[Claims] 1. Red light (wavelength 60
A method for collecting seedlings using glue, which comprises irradiating the seedlings with 0 to 700 mμ) to form sporangia, and obtaining a large amount of mature sporicidal spores from the sporangia in a short period of time.