JPS5951264B2 - Method for producing oligosaccharide-containing powder - Google Patents

Description

[Detailed description of the invention] The present invention relates to a method for producing oligosaccharide-containing powder.

The “oligosaccharide” mentioned here is galactosyllactose,
It refers to oligosaccharides of trisaccharides or more with high hygroscopicity, such as maltotriose and fucosyllactose.

Many of these oligosaccharides exhibit excellent growth-promoting effects on Bifidobacterium, which has recently attracted attention as a physiologically useful bacterium, and are therefore expected to have promising applications in powdered milk for infants and other dairy products. has been done.

However, since these oligosaccharides have high hygroscopicity, when they are added to powdered milk, for example, they have the disadvantage of significantly impairing the storage stability of the milk powder.

Conventionally, as a means to improve the fluidity of highly hygroscopic saccharides of disaccharides or less, proteins have been added to solutions containing these saccharides, or lactose, dextrin, starch, or grain flour has been added and the solution has been spray-dried. It has been proposed to powder it (Japanese Patent Publication No. 40-861, Japanese Patent Publication No. 49-44332).

However, even if these methods are directly applied to powderize solutions containing oligosaccharides of trisaccharides or more, it is not possible to obtain oligosaccharide-containing powders that simultaneously satisfy sufficient fluidity and solubility.

In view of the above-mentioned current situation, the present inventor has developed the present invention as a result of studying a method for producing an oligosaccharide-containing powder that improves the fluidity of the oligosaccharide itself and does not substantially impair solubility. reached.

Therefore, an object of the present invention is to provide a method for producing an oligosaccharide-containing powder with excellent fluidity and solubility.

The present invention will be explained in detail below.

The present invention is characterized in that an oligosaccharide-containing solution contains milk protein and lactose in a total amount (by weight) of at least about 2.8 times the oligosaccharide content in the solution, and A solution in which the weight ratio of milk protein to lactose is adjusted to 2:1 to 2:10 is powdered by spray drying.

The oligosaccharide-containing solution used in the present invention contains, in addition to the purified oligosaccharide solution, an aqueous solution of purified oligosaccharide powder obtained by concentrating and freeze-drying the oligosaccharide solution, and monosaccharides and disaccharides. This includes crude oligosaccharide solutions and mixed sugar solutions.

In addition, as a crude oligosaccharide solution containing monosaccharides and disaccharides, for example, 10 to 50 oligosaccharides obtained by treating an aqueous solution of sugar such as lactose with an enzyme (such as lactase) are used.
An example may be a sugar solution containing % by weight.

In the present invention, a solution containing oligosaccharides as described above contains milk protein and lactose in a total amount of at least about 2.8 times by weight relative to the oligosaccharide content in the solution.

Examples of the milk protein used here include buttermilk, cheese whey, skim milk, raw milk, and an aqueous casein solution obtained by adding an alkali salt of casein and calcium hydroxide, which may be used alone or in combination of two or more. Use as a solution in a mixture of

In addition, lactose may be commercially available, or even lactose-containing products such as skim milk powder may be used as a solution.

As described above, in the present invention, these milk protein sources and lactose (or lactose sources) are added to a solution containing oligosaccharides at a weight ratio of about 2 as the total amount of milk protein and lactose to the oligosaccharide content in the solution. .It is necessary to contain the above milk protein source and/or lactose (or lactose source) by 8 times or more, and to make the content weight ratio of milk protein and lactose in the solution 2:1 to 2:10. When using this, the amount of the added substance to be added is adjusted based on the oligosaccharide content in the target oligosaccharide-containing solution, taking into account the amount of milk protein and lactose in the above-mentioned additive.

In addition, unreacted lactose remains in the crude oligosaccharide solution obtained by treating lactose with an enzyme (such as lactase), so when adding lactose or a lactose source to this, the above-mentioned lactose It is necessary to consider the remaining amount of

1 The technical basis for adjusting the amounts of milk protein and lactose contained in the oligosaccharide-containing solution so as to meet the above conditions in the present invention is based on the experimental results shown below.

In the experiment shown here, the fluidity and water solubility of each powder obtained by spray drying were measured using various ratios of oligosaccharide, milk protein, and lactose in the oligosaccharide-containing solution to be spray dried. It is something.

The results are shown below.

Note that (%) in the table indicates weight.

△・・・・・・0.25～0.5ml x・・・・・・
・Represents 0.5m1 or more.

ADMI modified method: Take 10 g of the sample into a dry, clean 200 ml beaker, add 80° C. warm water (100 ml) in a graduated cylinder while stirring the contents with a dissolution test spoon, and dissolve.

Stirring was performed for 30 seconds (90 times) at a speed of 3 times/second, and immediately 50 ml was added to two centrifuge tubes for insoluble matter measurement using the ADMI method.
Fill to the line and then read the volume of the precipitate after centrifuging for 5 minutes at the specified speed.

Insoluble matter is determined by the average value of two readings.

(Note) The rotational speed of the centrifuge shall be the specified rotational speed of ADMI, but since the centrifugal force varies depending on the diameter, a predetermined correction will be made depending on the diameter.

(to ADMI') r, pom = 254m 7m
(based on 1074 rotations/rotation) As seen in the above experimental results, the total amount of milk protein and lactose relative to the oligosaccharide content in the oligosaccharide-containing solution to be powdered by spray drying is approximately 2.8. and the content weight ratio of milk protein and lactose in the solution is 2:
When powdering an oligosaccharide-containing solution in the range of 1 to 2:10, the resulting powder product has good fluidity and solubility, but when both of the above conditions are lacking, that is, when the milk protein and Even if the total amount of lactose is 2.8 times or more, 3 or 9 times the oligosaccharide content, if the content weight ratio of milk protein and lactose is outside the above range, or the content weight of the milk protein and lactose is Even if the ratio is within the above range, the total amount is less than about 2.8 times the content of the above oligosaccharides (2
, 2 times, and 2.6 times), it becomes impossible to satisfy both the fluidity and solubility of the powder product at the same time.

Incidentally, according to the present invention, in the process of powderizing an oligosaccharide-containing solution, it is possible to prevent the solution from adhering to the drying equipment and solidify and from browning of the oligosaccharide, and the drying operation can be easily managed. If the above conditions are not met, the above-mentioned adhesion and solidification and browning of oligosaccharides are unavoidable.

As described above, according to the present invention, by adjusting the contents of milk protein and lactose in the oligosaccharide-containing solution to be powdered, an oligosaccharide-containing powder having good fluidity and solubility can be obtained. I think that there is a great deal of use of oligosaccharides.

EXAMPLES The present invention will be explained in more detail with reference to Examples below.

Note that % in the examples indicates weight unless otherwise specified.

Example 1 Purified oligosaccharide liquid (solid content 20%, containing 100% galactosyllactose in solid content) 125 kg of skim milk (solid content 8.8%, containing 3.2% protein and 4.7% lactose) 300 kg and sodium caseinate powder (solid content 9
7%, containing 88.5% protein) and 37.6 kg of lactose powder (solid content 95%) were added and mixed to prepare an oligosaccharide-containing solution with a solid content of 20.5%.

The total amount of milk protein and lactose was 2.8 times the oligosaccharide content in the solution, and the ratio of milk protein to lactose was 2:5.

After concentrating the oligosaccharide-containing solution, the oligosaccharide-containing solution was spray-dried using a conventional method to obtain an oligosaccharide-containing powder of 95. I got Okg.

The powder has a solids content of 98.4%, 20% milk protein, 50% lactose, 25% oligosaccharides, 2.9% ash and 0.5% fat.
%, and had good fluidity and solubility in water.

Example 2 Crude oligosaccharide liquid obtained by acting lactase on lactose (30% solid content, containing 4.8% monosaccharides, 19.6% galactosyllactose, and 5.6% lactose) 43.4 kg
and 12.1 kg of purified oligosaccharide liquid (70% solid content, containing 90% or more maltotriose in solid content),
Raw milk (solid content 12.1%, milk protein 3
．． 1% and 4.6% lactose) and 82 kg of skim milk powder (97% solids, 35.8% milk protein and 52% lactose).
4%) was added and dissolved.

A solution of lactose (solid content 95%) dissolved in 36.8 kg of tap water was added to the obtained mixed solution and mixed until the solid content was 40%.
% oligosaccharide-containing solution was obtained.

The total amount of milk protein and lactose was 4.2 times the oligosaccharide content in this oligosaccharide-containing solution, and the ratio of milk protein and lactose was 2:10.

The above oligosaccharide-containing sugar solution was spray-dried by a conventional method to obtain 93.5 kg of oligosaccharide-containing powder.

This powder has a solids content of 97%, milk protein 12%, and lactose 6%.
It had a composition of 0%, oligosaccharide 17%, ash 2.7%, fat 3.2% and monosaccharide 2.1%, and had good fluidity and solubility.

Example 3 230 kg of purified oligosaccharide liquid (10% solid content, containing 100% galactosyllactose), 0.9 kg of sucrose powder, and casein powder (88% solid content, containing 84% milk protein) 27.4kg lactose (95% solids)
) 50.5 kg and potassium carbonate 1. Okg was added and mixed to prepare an oligosaccharide-containing solution with a solid content of 31.3%.

The total amount of milk protein and lactose was 3.1 times the oligosaccharide content in this oligosaccharide-containing solution, and the ratio of milk protein to lactose was 2:4.2.

The oligosaccharide-containing solution was concentrated and then spray-dried using a conventional method to obtain 92 kg of oligosaccharide-containing powder.

This powder has a solids content of 97%, milk protein 23%, and lactose 48%.
%, oligosaccharide 23%, fat 0.6%, ash 1.5% and sucrose 0.9%, and has good fluidity and solubility.

tuna.

Example 4 Purified oligosaccharide liquid (10% solid content, containing 100% maltotriose in solid content) 100 kg Nisyo sugar 5.15 k
Add and dissolve buttermilk solution (solid content 6.5%, containing 2.3% milk protein and 3.2% lactose) to the dissolved sugar solution.
660.8kg and lactose (95% solids) 40.7k
An oligosaccharide-containing solution with a solid content of 12% was prepared by adding a mixed solution of g.

The total amount of milk protein and lactose was 7.5 times the oligosaccharide content in this oligosaccharide-containing solution, and the ratio of milk protein and lactose was 2:8.

After concentrating the oligosaccharide-containing solution, it was spray-dried by a conventional method to obtain 94 kg of oligosaccharide-containing powder.

This powder has a solids content of 97%, milk protein 15%, and lactose 60%.
%, oligosaccharide 10%, ash 3.7%, fat 3.1%, and sucrose 5.2%.Both fluidity and solubility were good.

Claims (1)

[Claims] 1. When producing an oligosaccharide-containing powder by spray drying a solution containing oligosaccharides, add milk protein and lactose to the solution containing oligosaccharides in a total amount based on the oligosaccharide content in the solution. A method for producing an oligosaccharide-containing powder, characterized in that the powder contains at least about 2.8 times the amount (by weight), and the weight ratio of milk protein to lactose in the solution is adjusted to 2:1 to 2:10. .