JPS5953026B2 - waste liquid equipment - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a liquid waste device for disposing of waste liquid in a culture container used when subculturing tissues or cells.

In all fields such as medicine, biology, pharmacy, and agriculture,
Biological tissue and cell culture techniques are essential basic experimental techniques for conducting research at the cellular level.

However, subculturing biological tissues and cells is technically difficult, and stable culture strains have not been obtained. However, recently, with the spread of gas culture technology in incubators, that is, culture technology in a specific gas atmosphere, special cells that were previously considered difficult to grow, such as those of the liver, nervous system, and pituitary gland, have become more popular. Even subculture is becoming possible.

'The main points of these current cell methods are described below.

First, in order to carry out subculture, a predetermined number of cells are diluted with a culture solution in a culture container such as a Petri dish, and the cells are cultured stationary in an incubator maintained in a predetermined atmosphere. In order to check the culture state after a predetermined period of time has elapsed, a predetermined culture container is removed from the incubator and the degree of cell proliferation is examined using a microscope. When it is confirmed that the target cells have grown to the full capacity of the specified container, the cells are transferred to sterile clean pliers, and the culture solution in the culture container is aspirated with a pipette and discarded. The cells remaining in the container are washed by injecting a buffer solution, and the buffer used for this washing is again sucked and discarded. Next, an enzyme such as trypsin is injected to release the cells that have settled and proliferated on the floor of the culture container from the culture container, and the cells are left for several minutes and then discarded. After allowing it to stand for a few more minutes, the culture solution is injected. This culture solution has the effect of stopping the action of trypsin. After injecting the culture solution, stir and transfer to a centrifuge tube for centrifugation. Aspirate and discard the centrifuged supernatant. Next, a culture solution is injected and stirred to resuspend the cells, and the resuspended cell suspension is dispensed in fixed amounts into a new culture container, and the culture solution is further injected to a predetermined concentration. After the dilution and dispensing operation has been completed in this manner, the culture container is taken out using clean pliers, transferred to an incubator maintained at a predetermined atmosphere, and left to stand still to proceed with culture again. However, the method described above has the following drawbacks.

One of them is that in order to check the growth state of tissues or cells using a microscope, it is necessary to frequently remove the culture container from the incubator to the outside air.

Therefore, when the culture condition is taken out from the predetermined environmental conditions such as gas atmosphere, temperature, humidity, etc. into the outside air, the culture conditions may change suddenly, and subtle changes may occur in the cultured tissue or cells. Also, since it comes in contact with the outside air, it is susceptible to contamination by bacteria. In this way, it is directly affected by changes in environmental conditions and the contamination of various bacteria. Next, based on the results of microscopic observation, the technician manually performs the above-mentioned subculture operation in a clean bench, so there is a direct effect on tissues or cells during the subculture operation of the technician. .

In other words, this shows that it is not possible to perform standardized culture under certain conditions with such conventional subculture operations. In addition, the cultured tissue fibers or cells
It will depend on the technician's experience and skills. This means that it is difficult to standardize and unify the culture technology itself. For this reason, even when researchers conduct research on the same topic, it is often seen that researchers come to completely opposite conclusions. Furthermore, because it takes a considerable amount of time to train technicians equipped with these culture techniques, there is a shortage of personnel, and researchers are unable to devote their full efforts to their original research, and they are forced to focus on branch culture techniques. The reality is that you have to spend a lot of energy.

From the above, it is possible to prevent contamination of outside air by conventional methods, eliminate the influence of human manipulation,
Further, automatic culture apparatuses are being developed for the purpose of automatically subculturing standardized tissues or cells by standardizing and unifying each operation in subculture. In such an automatic culture device, as already explained, it is necessary to discard the culture solution, buffer solution, etc. in the culture container, and one possible method for this purpose is to aspirate and discard the solution with a pipette.

However, this method requires a pipette to aspirate and a device to operate the waste liquid using the pipette, and if the same pipette is used repeatedly to operate the waste liquid, there is a problem of contamination. In order to prevent this, it is necessary to replace the pipette each time, which is inconvenient. In view of the above points, the present invention provides a culture vessel waste liquid device which allows culture vessels to be disposed of by tilting them, and which prevents contamination during disposal.

The specific content of the present invention will be explained below based on the illustrated embodiments. Figure 1 is a side view, Figure 2 is a plan view, and Figure 3 is a side view.
The figures are cross-sectional views taken along the line 111-111, and in these figures, 1 is a culture container, and 2 is a holding stand, which is made of a plate-like member, and most of the center part is hollowed out except for the ends at both ends. As shown in the figure, the culture container 1 is placed on this portion.

Reference numeral 3 denotes holding plates, which are attached to both sides of the groove plate 4, and whose tips are structured to hold down the peripheral portion of the culture container 1.

As shown in FIG. 3, the groove plate 4 has a V-groove shape with a lower center portion. The other end of this groove plate is fixed to a support member 6 rotatably attached to the support shaft 5. One end of the holding table 2 is rotatably attached to a shaft 5 and can be rotated about this shaft 5 by an appropriate method, or it can be fixed to this shaft 5 so that it can be rotated by the rotation of the shaft 5. It is composed of Reference numeral 7 indicates a spring attached to the holding table 2 and the holding plate 3, which pulls the holding plate 3 in the direction of the holding table 2, that is, in the direction of the arrow in FIG.

Reference numeral 8 denotes a pin that is attached so as to be movable in the direction of the arrow in FIG. .

9 is a waste liquid tank.

Next, the operation of the waste liquid device having the above structure will be explained.

First, use an appropriate moving means to place the culture container 1 on the tip of the holding table 2 as shown in the figure, and then move the pin 8 and remove it from under the holding plate 3 and the groove plate 4. The holding plate 3 is moved toward the holding base 2. Thereby, the culture container 1 placed on the tip of the holding table 2 is fixed by the presser plate 3 so that it cannot move. In this state, if it is rotated around the shaft 5, the tip of the holding table 2 will be moved to A, B, C in sequence as shown by the chain line in FIG. 2, along with the culture container.
. . . When moving from B to C, the waste liquid in the culture container, such as the culture solution, jumps out and is disposed of in the waste liquid tank 9. At the same time, when the culture container is held at a position like C, the waste liquid at the bottom of the culture container flows out, passes through the V-groove of the groove plate 4, and is disposed of in the waste liquid tank. Next, rotate the holding stand in the opposite direction and return it. At this time, the holding base 2 rotates counterclockwise around the shaft 5 as shown in FIG. When inserted between the pin 8 and the plate 3, the presser plate 3 stops when it touches the pin 8, and only the holder 2 rotates, resulting in the state shown in FIG. In other words, the presser plate 3 can be moved away from the culture container 1. Therefore, the culture container can be removed from the holding table 2. As explained above, the liquid waste device of the present invention does not require a pipette or other disposal device, and the culture container itself is disposed of by tilting it, and the waste liquid at the bottom of the culture container can also be disposed of through the groove plate. Can be discarded.

Furthermore, the waste liquid is disposed of through the structure plate, and the holding table 2 is not contaminated.

[Brief explanation of the drawing]

1 and 2 are a side view and a plan view, respectively, of the liquid waste device of the present invention, and FIG. 3 is a sectional view taken along the line 111-111 in FIG. 2. 1...Culture container, 2...Holding stand, 3.
...Press plate, 4...Groove plate.

Claims (1)

[Claims] 1. A holding stand whose tip part has a structure on which a culture container can be placed, and whose other end part is supported so as to be rotatable around an axis;
A grooved plate having a V-shaped groove, fixed to both sides of the grooved plate, one end of which is configured to press down a culture container placed on a holding stand, and the other end rotates together with the grooved plate about the axis. A culture container is placed on the holding table and is pressed and fixed by the holding plate, and then the holding table, the holding plate, and the groove plate are attached to the shaft. A waste liquid device characterized in that the waste liquid in the culture vessel is disposed of by rotating the culture vessel around the plate, and the waste liquid flowing out from the culture vessel is disposed of by passing through the V-shaped groove of the groove plate.