JPS60180588A - Preparation of immobilized complex of microorganism having plant pathogen-controlling activity - Google Patents

Abstract

PURPOSE:To obtain an immobilized complex of microorganisms having strong controlling effect to the plant blights, by treating a solution containing microorganisms having antagonistic activity to plant pathogen, and reacting the solution with carrageenan. CONSTITUTION:A solution containing microorganisms having antagonistic activity to plant pathogen, is treated with a mineral acid salt of a bivalent or polyvalent metal. The obtain solution is made to react with carrageenan, and the reaction product is dried. The microorganism used in the above process is e.g. bacteria, actinomycetes, eumycetes, myxomycetes, lichens, algae, etc. The culture of the microorganism can be carred out e.g. by the shake culture in liquid medium, stationary culture in liquid medium, and the culture in solid medium. The bivalent metal mineral acid salt is e.g. calcium chloride, magnesium chloride, barium chloride, etc. The obtained complex having the activity to control the plant pathogen exhibits strong controlling effect to the blights of plant, especially against the plant pathogen infecting via soil. Furthermore, the complex can be stored stably for a long period, and is advantageous in view of the quality control.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for producing an immobilized microorganism complex having plant pathogen control activity.

In recent years, methods have been proposed in which not only enzymes are immobilized, but also bacterial cells are immobilized as they are, but attempts have been made in both cases to carry out enzyme reactions continuously. However, it has not yet been tested for the purpose of controlling plant diseases.

Conventional immobilization of bacterial cells (11 carrier binding method, (2) cross-linking method, (3) comprehensive method ("Enzyme Engineering", edited by Fukui Sanbe et al., p. 15)
8-202), etc. However, as a result of intensive research on various known immobilization methods such as those mentioned above, the present inventors have found that microorganisms having antagonistic properties against plant pathogenic bacteria (hereinafter referred to as microorganisms) can be added to a culture solution. By adding divalent or higher metal mineral salts, dissolving carrageen in the solution thus obtained, and drying, it is possible to obtain microscopic immobilized microorganisms containing microorganisms as their core. This immobilized microorganism has a strong control effect against plant diseases, and the present invention was completed based on this knowledge.

That is, according to the present invention, a complex having plant pathogen control activity is obtained by culturing microorganisms with shaking in a liquid medium according to a conventional method, and pulverizing the culture together with the liquid medium in a mixer.
By adding gold with a valence of 2 or higher to the solution and immobilizing it, an immobilized microorganism is produced, and then by drying this, the above-mentioned active composite can be produced.

The microorganisms that can be used in the present invention are not particularly limited as long as they have antagonistic properties against plant pathogens, and any of bacteria, actinomycetes, fungi, subtilis, lichens, and algae can be used. These microorganisms may be living cells, or may be freeze-dried, freeze-thawed, or the like.

The microorganisms used in the present invention may be of a single type, but depending on the intended target, they may be of two or more types of the same species (in the past, two or more types of microorganisms of different species may be used at the same time). .

The microorganisms used in the present invention may be cultured in liquid shaking culture, static liquid culture, or solid culture.

In the present invention, suitable examples of carrageenan include Neusolin (trade name manufactured by Chuo Kasei Co., Ltd.), Denyu rA/WG (Copenhagen Pectin Fac) I
(trade name manufactured by J-Company), rNewgel OWG (same as above), and the like.

Examples of the divalent or higher-valent metal mineral salts used in the present invention include calcium chloride, magnesium chloride, barium chloride, ferrous chloride, ferric chloride, manganese chloride, and the like.

The complex having plant pathogen control activity obtained by the method of the present invention shows a strong control effect against plant diseases, especially soil-borne plant pathogens, and is stable even when stored for a long time. It is also advantageous in terms of quality control.

EXAMPLES The present invention will be described below with reference to Examples, but the present invention is not limited in any way by these Examples.

Example 1 Medium 10Qm11 with pH 7.0 containing 0.5% peptone, 01.1% potassium phosphate, 0.05% magnesium sulfate, and 1% sugar! After sterilization, the soil-borne plant pathogen Fudelium (Fuea
Bacillus licheniformis (Bacillus lichenif
ormis) and cultured with shaking at 28°C. After treating the bacterial cells by adding 6-caliber calcium chloride to the culture solution at a concentration of 0.1M, carrageenan was added to 5%.
Add and mix well. By spray drying the solution thus obtained, dried immobilized microorganisms 10 to 15.9 were obtained.

Example 2 Soil-borne plant pathogen Corticium rolfsii (Co
Aspergillus tellurium, which is antagonistic to Aspergillus rolfsii)
terreus).

Trichoderma vi
ride) 4 A culture cultured in the same manner as in Example 1 or a statically cultured culture is pulverized with a mixer, and the thus obtained culture is produced in the same manner as in Example 1 to obtain 60 immobilized microorganisms. ~40g obtained.

Example: Stability over time of immobilized microorganisms produced in the same manner as in Examples 1 and 2. The results are shown in Table 1. The stability of each microorganism was calculated by separating the number of remaining bacteria after microorganism preparation using a 2-dilution plate method.

Example 4 The effect of immobilized microorganisms prepared in the same manner as in Example 1 on controlling σ cucumber vine disease was investigated. The results of B are shown in Table 2.

Namely, the fungus Fusarium oxysporum f, s
p.

cucumerinum) and inoculated into diluvial soil at a number of 15 to 6 x 106 bacteria per 1z of soft soil.The immobilized microorganisms were mixed using 11 cloud heads per flower pot with a diameter of 15 crn, and then cucumber seeds were sown. The effect on the occurrence of vine diseases was investigated. The experiment used 1 ward 1 [boop] Y, 1y+? The cultivation was carried out by sowing 10 seeds per pot and incubating them 6 times.

The disease incidence is expressed as the number of wilted and dead individuals, and the control plot is diluvial soil inoculated with the furnace fungus.

Control value 1 - Calculated as follows.

In addition, the numerical values shown in the table were investigated on the 21st day after seeding.

Table 2 ) hmm Agent Asamura Hajime

Claims (1)

[Claims] It is characterized by treating a solution containing microorganisms that are antagonistic to plant pathogens with a divalent or higher metal mineral salt, reacting with the resulting solution of carrageenan, and drying the reactant. A method for producing an immobilized microorganism complex having plant pathogen control activity.