JPS6019725B2 - skin cosmetics - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a skin cosmetic having excellent effects on skin health, beautification, protection, and hydration.

More specifically, the present invention relates to hyaluronan. The present invention relates to a skin cosmetic containing one or more types of phosphoric acid, chondroitin 4-sulfate, chondroitin-6 sulfate, or salts thereof, and one or more types of soluble collagen. Conventionally, it has been considered ideal for general skin cosmetics to reproduce on the skin almost the same components as those on the skin surface, and most of them are mainly composed of water and oil, which are the typical components of the skin. However, in recent years, a report was made by D. Jacobi who systematized it as the NMF theory of human skin, and NMF (Natural
The stratum corneum of healthy human skin contains 30-40% of a series of water- and polar solvent-soluble substances called Moisturizing Factors, such as amino acids, pyrrolidone carboxylic acid (salts), lactate, and urea. Furthermore, the flexibility of the skin and its functions are influenced by the moisture content of the stratum corneum, which in turn depends on the content of specific water-removing contents of the stratum corneum, especially NMF. The ingredient has come to be recognized as a moisture-absorbing ingredient.

However, although such moisture-absorbing ingredients can perform their functions at high temperatures, at low temperatures their effect of absorbing moisture from the skin becomes extremely large, and as a result, the skin surface becomes This causes a large amount of water to be lost from the water, and rather, the function is not fully utilized, resulting in a major drawback. Some attempts have been made to solve this drawback, such as by using sodium pyrrolidone carboxylate and polypeptide, but no satisfactory method has yet been achieved. On the other hand, from the perspective of dermatology, it is known that the aging phenomenon of the skin can be roughly divided into aging of skin cells and aging of the dermal connective tissue.

There are two major connective tissue components in the summer skin: a fiber component and a matrix component. That is, the skin intercellular matrix is formed by crosslinked collagen fibers formed into helices by three peptides and mucopolysaccharide, which is a proteoglycan.
In addition, the skin fibers of healthy, young humans have a high proportion of soluble collagen, while in mature and aged human skin, a large amount of non-sealike collagen is produced due to increased intramolecular cross-linking of soluble collagen. . Matrix components, especially hyaluronic acid, decrease in inverse proportion to the accumulation of insoluble collagen, and as a result, with aging, skin tissue loses its flexibility and elasticity, and its water retention capacity decreases, leading to increased wrinkles in the skin. In order to prevent this skin aging phenomenon or to give a rejuvenating effect to the foot of the skin, a bamboo paste containing active ingredients of the skin dermis has been proposed. (sulfuric acid), mucoitin sulfate, caronine sulfate (Hakama Kosho 32-2700, Mochikosho 33
Cosmetics containing mucopolyamides such as A-50 Ikari (see Japanese Patent Publication No. 50-50 Ikari), and cosmetics containing soluble collagen, polypeptide (Japanese Patent Publication No. 52-8-1024, Japanese Unexamined Patent Publication No. 48-10240), etc.

However, the active ingredients that have been studied so far have focused solely on their individual effects, and no attention has been paid to interactions or synergistic effects with other ingredients. In fact, in the case of mucopolysaccharides, almost no hygroscopicity or water-encapsulating properties were observed when used alone, and at the same time, when used alone, both mucopolysaccharides and soluble collagen exhibited the same drawbacks as the NMF component described above. It is also generally known that when a substance that is the same as its constituent components in the skin is applied externally, beneficial results are not necessarily obtained, and furthermore, soluble collagen is easily denatured by heat etc., reducing its effectiveness. This is also an obvious fact. As a result of various systematic studies aimed at solving the above-mentioned drawbacks, the present inventor discovered that a cosmetic containing a specific mucopolysaccharide or its salts and soluble collagen has a health-beautifying and hydrating effect on skin dust. The present inventors have succeeded in providing the cosmetic composition of the present invention by discovering that it can exert excellent efficacy and also reliably eliminate the above-mentioned disadvantageous adverse effect on the skin.

That is, the present invention achieves the following objectives {1' to {4): '1' To significantly increase the water-wrapping properties of skin lamps, soften the skin, and increase elasticity {2) Especially at low temperatures. {3} Prevents denaturation of soluble collagen ■ It does not irritate the skin even when used in large amounts for a long time, does not inhibit physiological functions, and does not irritate the skin. Our goal is to provide cosmetics that protect and beautify.

The present invention will be explained in detail below.

The present invention comprises the following components (1) and (2): (1) one or more components selected from hyaluronic acid, chondroitin 4-sulfate, chondroitin-6 sulfate, or salts thereof; and (1) one or more soluble collagens. The present invention provides a skin cosmetic product characterized by containing at least one of the above ingredients, wherein the @ component is 0.001 to 5% by weight, and the component (■) is 0.01 to 5% by weight. The components (1) and (2) are contained in a ratio of 0.1:5 to 5:1.

The cosmetic composition of the present invention is characterized by containing both the above-mentioned ingredients (1) and (2), and other ingredients may be contained therein. .

In a preferred embodiment of the present invention, at least one of Q-amino acid and pyrrolidone carboxylic acid or a salt thereof is used as such other components. It is preferable to contain such a component in an amount of 0.01 to 5% by weight, and it is preferable to contain the component under conditions of use such that the dew point is equal to or higher than the same dew point. When the above component (■) is used alone, almost no hygroscopicity or water-envelopment is observed, and it is only when it is present together with the component (2) that it brings about the effect that meets the purpose of the present invention. . Even among mucopolysaccharides, such effects are not particularly observed in other mucopolysaccharides such as dermatan sulfate, chondroitin 4,6 disulfate, dermatan 4,6 disulfate, keratan sulfate, and hebaran sulfate. In the cosmetics of the present invention, the soluble collagen used is one extracted with a weakly acidic, weakly alkaline, or neutral salt aqueous solution by a known extraction method (for example, see Tokkoku Sho 49-134810 and Tokkoku Sho 52-8384). be able to. As the Q-0 amino acid, pyrrolidone carboxylic acid, and salts thereof, those commonly used for skin cosmetic purposes are used. The preferred concentration of each of these ingredients is as follows:
The component (2) is 0.02 to 3% by weight, the component (2) (soluble collagen) is 0.1 to 3% by weight, and the Q-quami/acid pyrocarbon carboxylic acid is 0.1 to 3% by weight. Next, in order to prove the effects and usefulness of the present invention, the above-mentioned component (■) and
The results of a test to measure water encapsulation and skin elasticity on the elbow surface of rat skin were conducted using a mixed system with these components and a system using these components alone.

1) Samples Hyaluronic acid, lochondroitin 4 sulfate, hadermatan sulfate, chondroitin 6 sulfate, soluble collagen, hepyrrolidone carboxylic acid sodium salt, tocuserin sodium salt, thipropylene glycol Table 1 below contains one of each of the above samples. Or a list of specimens obtained using two or more types of stool*.

(Each number in the table is the working concentration (weight%) of each sample in aqueous solution.
) is shown. ) Table 1 *1 Sample 12 is sample 8 aged at 40°C for 2 months in Suifu "*
Sample 2 13 is an aqueous solution obtained by aging sample 4 at 40°C for 2 months and then adding 2% chondroitin 6 sulfate.

2) Experimental method [Experiment a] A group of 5 Wistar rats, approximately 4 weeks old, were prepared, and each rat was treated with 3x per day on the dorsal surface.
An operation of uniformly applying 0.1 coat of each sample per 3 skins for 4 consecutive weeks was performed on the condition that the samples were gently wiped off every weekend.

Next, the rat skin (average thickness 0.5 skin) was peeled and cut into 3.5 skin diameter pieces, and dried until the skin reached a constant weight in a large desiccator with relative humidity adjusted to 0% with concentrated sulfuric acid. let Thereafter, the upper part of a weighing bottle with an inner diameter of 3 and a depth of 3 arcs, which was filled with physiological saline to one skin level, was then covered and fixed with the treated skin. Furthermore, a desiccator (effective diameter 2%) with relative humidity of 20, 50, and 9% adjusted with concentrated sulfuric acid and water prepared separately.
The skin set in a weighing bottle was quickly placed in each of five weighing bottles, the upper part of the desiccator was sealed, and the change in weight of each sample was measured and compared after 4 hours. 2nd below
The unit of the result 'a} in the table is 9, and a negative value indicates weight loss. (Speaker) Only one group of sheep is not intended to be coated with any sample at all.

[Experiment b]

The skin of a rat having a thickness of 0.5 ribs treated with the same specimen as in Experiment A was cut into 1×2 pieces, and the skin was subjected to a tensile test using a modified tensilon meter.

The elasticity values in Table 2 are the area of the hysteresis curve drawn when the skin is stretched to the median value between the first and second descending points and then contracted, and the elasticity value is the area of the hysteresis curve drawn when the skin is stretched to the median value between the first and second descending points, and the blank (untreated skin) is 100
It shows the percentage when the number is 4.
Sashimi is highly elastic. As mentioned above, in both [Experiment A] and [Experiment B] after skin exfoliation, the relative humidity was 2 0, and the relative humidity was 5.
The test was carried out in a constant temperature room with a constant temperature of 0%. Furthermore, all the numerical results are average values. Table 2 3) Results As shown in Table 2, hyaluronic acid, mucopolysaccharide, and soluble collagen have poor effective water enveloping ability when used alone, and amino acids also lose their water enveloping ability at low humidity.

However, it is clearly recognized that in the mixed system of soluble collagen and chondroitin 4-sulfate or chondroitin-6 sulfate, excellent water envelopment continues down to low temperatures.
In addition, it has been confirmed that sample 13, in which soluble collagen was treated at high temperature and then chondroitin 6 sulfate was added thereto, had lower water envelopment than sample 12, in which chondroitin 6 sulfate was added to soluble collagen and treated at high temperature from the beginning.

This indicates that chondroitin 6 sulfate prevents denaturation of soluble collagen in the system, and in fact, as a result of actual analysis, a large amount of collagen denaturation due to heat treatment of soluble collagen was observed in sample 13. Furthermore, the results of the skin tensile test (experiment b) revealed that a mixed system of the component (1) and the component (2) was effective in improving the elasticity of the skin.

Furthermore, it was also observed that the elasticity of the skin when using a specimen heat-treated with a mixed system of soluble collagen and chondroitin hexasulfate was not lowered compared to that of an untreated specimen. It was confirmed that hyaluronic acid has the same effect as chondroitin 4-sulfate and chondroitin-6 sulfate. Next, in comparing the nutritional cream of Example 1 described later with a conventional nutritional cream, the effect of application was investigated by applying it to 60 adult women for 3 months in winter (approximately 1 skin area once every night), as shown in Table 3. The results were obtained.

Table 3 After keeping it at 40℃ for 2 months, using a nutritional cream with 3.0% chondroitin 6 sulfate added Based on the results in Table 3, a x2 test is performed to find that the effect of moisturizing the skin is X, Y, and X. and Z both have a risk rate of 1%
A significant difference was observed, indicating that there is a difference between nutritional creams.

In addition, regarding the effect of preventing rough skin on the skin of the elbow, there is a risk rate of 5% between X and Y, and a risk rate of 1% between X and Z. A significant difference was observed at a risk rate of 1%. The above results demonstrate that the nutritional cream of the present invention is clearly more effective for the skin than creams that use only soluble collagen, and also has the effect of preventing collagen degeneration over time. did. In addition, the emulsion W of Prescription Example 2 described later, the conventional emulsion Y of the formulation from which chondroitin 4 sulfate and sodium hyaluronate have been removed, and the emulsion of Prescription Example 2
02 months old X, and conventional emulsion 40
After 2 months of treatment, Z containing 0.5% each of chondroitin 4 sulfate and sodium hyaluronate was administered to 60 adult women for 1 month during the winter (twice daily, totaling approximately 1% When the application effect was investigated by applying it repeatedly to the skin, the results shown in Table 4-1 were obtained.

Based on the results in Table 4-1, if the test is carried out, the result will be as shown in Table 4-2.

In other words, for the effect of moisturizing the skin and preventing skin roughness, there is a risk rate of 1% between W and Y, W and Z, and X and Y.
A significant difference was observed between X and Z at a risk rate of 5%.

Also, regarding the effect of softening the skin, W and Y, W and Z
Significant differences were observed between X and Y, and between X and Z with a risk of 5%. In this way, the emulsion which is an example of the present invention is obtained by adding 4 pi○ of emulsion Y and Y containing only soluble collagen.
After 2 months, it was found that the emulsion Z to which chondroitin 4 sulfate and sodium hyaluronate were added was clearly more effective on the skin. On the other hand, no significant difference is observed between W and Y.

This revealed that the emulsion according to the present invention also has the effect of preventing collagen degeneration over time. Fourth
-1 Table 4-2 (Significant difference test results) W x YZ (Note) **: Risk rate 1 grandson *: Risk rate 5 Purple -: No significant difference Furthermore, hand cream X of prescription example 6 below, this The results shown in Table 5 were obtained when 45 adult women were allowed to freely apply the conventional hand cream Y on their hands for one month during the winter to examine the effects of application.

Based on these results, a significant difference was found between X and Y at a risk rate of 1% in terms of the effect of moisturizing the skin and the effect of preventing rough hands. Furthermore, regarding the effect of softening the skin, a significant difference was observed between X and Y at a risk rate of 5%. Thus, it can be seen that the hand cream according to the present invention is clearly more effective for the skin than hand cream Y containing only sodium hyaluronate. As shown in Table 5 and above, the cosmetics of the present invention have properties that are extremely useful for the skin, and when the skin cosmetics of the present invention are used, they provide excellent water-wrapping properties to the skin even under low humidity. It activates the movement of skin cells, increases the flexibility and elasticity of the skin, keeps the skin healthy, gives a youthful and lustrous appearance, and even when used in large quantities, does not alter physiological functions. It does not cause any abrasions and can be used extremely safely.

In addition, if the amount of the soluble collagen, the component (2), and the mucopolysaccharide, the component (2), used in the cosmetic of the present invention are both greater than 5% by weight, the feeling of use and emulsifying property may be reduced. , and even in terms of effectiveness, they reach parallelism and are not particularly useful. Next, a formulation example of the skin cosmetic of the present invention will be shown.

The numerical value of the blending ratio of each component is % by weight. Example 1 Nutritional Cream C Fragrance Example 2 Emulsion Example 3 Lotion Example 4 Emulsifying Pack Example 5 Hair Cream Example 6 Hand Cream

Claims (1)

[Scope of Claims] 1. A skin cosmetic containing one or more of hyaluronic acid, chondroitin 4-sulfate, chondroitin-6 sulfate, or their salts, and one or more soluble collagen. 2. The skin cosmetic composition according to claim 1, which contains at least one of α-l amino acid and pyrrolidone carboxylic acid or a salt thereof.