JPS6022907B2 - Device that controls dissolved oxygen concentration in culture medium - Google Patents
Device that controls dissolved oxygen concentration in culture mediumInfo
- Publication number
- JPS6022907B2 JPS6022907B2 JP13353277A JP13353277A JPS6022907B2 JP S6022907 B2 JPS6022907 B2 JP S6022907B2 JP 13353277 A JP13353277 A JP 13353277A JP 13353277 A JP13353277 A JP 13353277A JP S6022907 B2 JPS6022907 B2 JP S6022907B2
- Authority
- JP
- Japan
- Prior art keywords
- oxygen concentration
- motor
- dissolved oxygen
- deviation
- culture solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Control Of Non-Electrical Variables (AREA)
Description
【発明の詳細な説明】
本発明は培養槽内に容れられた培養液内に溶存する酸素
濃度を制御する装置に関するものである。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a device for controlling the concentration of oxygen dissolved in a culture solution contained in a culture tank.
培養液の溶存酸素濃度の制御は培養系を制御するために
重要なことである。Controlling the dissolved oxygen concentration in the culture solution is important for controlling the culture system.
従来、このような酸素濃度を制御する手段として、培養
液中に送られる通気量を調節する方法、培養液中に送ら
れる通気の中の酸素分圧を調節する方法、培養槽中に設
けられた鷹梓機の回転速度を調節することによって培養
液と酸素の接触効率を制御する方法、培養液中に流加さ
れる基質の流加速度を調節する方法（連続培養の場合）
等があるが、実際上、通気量の調節によっては充分な効
果を得ることはできず、また、通気中の酸素分圧を調節
する方法は高濃度の酸素の使用を伴うために安全性等の
実際上の問題が多く工業的に使用し‘こくい。実際上、
酸素濃度を制御する方法としては、損梓機の回転速度を
調節する方法および基質の流加速度を調節する方法が有
効で、また、これらの方法は可変抵抗器によって鷹梓機
の駆動モータ或いは基質給送モータの回転数を調節する
ことによって、酸素濃度の制御を極めて簡単に行うこと
ができるので、好都合である。本発明は、このように可
変抵抗器の調節によって培養液内の溶存酸素濃度を極め
て簡単に制御することのできる装置を提供するもので、
培養液内の溶存酸素濃度を継続的に検出し、その酸素濃
度値が設定された基準酸素濃度値から上または下に外ず
れた時に上記の可変抵抗器を自動的に調節することによ
って酸素濃度を基準値に自動的に制御する酸素濃度制御
装置を提供しようとするものである。Conventionally, as means for controlling such oxygen concentration, there have been methods to adjust the amount of aeration sent into the culture solution, methods to adjust the partial pressure of oxygen in the aeration sent into the culture solution, and methods to adjust the oxygen partial pressure in the aeration sent into the culture solution. A method of controlling the contact efficiency between the culture solution and oxygen by adjusting the rotation speed of the Tataka Azusa machine, a method of adjusting the flow rate of the substrate added to the culture solution (in the case of continuous culture)
However, in practice, it is not possible to obtain a sufficient effect by adjusting the amount of ventilation, and the method of adjusting the oxygen partial pressure during ventilation involves the use of high concentration oxygen, so there are safety issues, etc. There are many practical problems that make it difficult to use it industrially. In practice,
Effective ways to control the oxygen concentration are to adjust the rotational speed of the hawk evaporator and to adjust the flow acceleration of the substrate. This is advantageous because the oxygen concentration can be controlled very easily by adjusting the rotational speed of the feed motor. The present invention thus provides a device that can extremely easily control the dissolved oxygen concentration in the culture medium by adjusting the variable resistor.
The oxygen concentration is determined by continuously detecting the dissolved oxygen concentration in the culture medium and automatically adjusting the variable resistor described above when the oxygen concentration value deviates above or below the set reference oxygen concentration value. The present invention aims to provide an oxygen concentration control device that automatically controls oxygen concentration to a reference value.
このような目的で、本発明は培養液内の酸素濃度を検出
しその酸素濃度値が或る設定された基準酸素濃度値から
上または下にずれた場合にその偏差を表示する偏差表示
装置と、この酸素濃度の偏差に応じて、この偏差を零と
する方向に、上記の蝿枠機の駆動モータまたは基質給送
モータ等の溶存酸素濃度を制御するモータの回転数を増
加または減少させるために、上記の可変抵抗器の抵抗値
を増加または減少させる可逆モータをいずれかの方向に
回転させるモータ回転方向功換装置と、上記の偏差値の
大きさに応じて該可逆モータの回転速度を増減させるモ
ータ回転速度制御装置とを備えたことを特徴とする培養
液の溶存酸素濃度を制御する装置を提供するのである。For this purpose, the present invention includes a deviation display device that detects the oxygen concentration in the culture solution and displays the deviation when the oxygen concentration value deviates above or below a preset reference oxygen concentration value. , to increase or decrease the rotational speed of the motor that controls the dissolved oxygen concentration, such as the drive motor of the fly frame machine or the substrate feeding motor, in a direction that makes this deviation zero, according to the deviation of the oxygen concentration. and a motor rotation direction switching device that rotates the reversible motor in either direction to increase or decrease the resistance value of the variable resistor, and a rotation speed of the reversible motor depending on the magnitude of the deviation value. The present invention provides a device for controlling the dissolved oxygen concentration of a culture solution, which is characterized by being equipped with a motor rotation speed control device that increases and decreases the concentration of dissolved oxygen in a culture solution.
以下、図面に示す本発明の実施態様について説明する。
図中、１は培養液を容れた培養槽、２はその中に収容さ
れた培養液、３は培養液中の溶存酸素濃度を検出する検
出器、４は該検出器によって検出された酸素濃度を表示
する溶存酸素計、５は培養槽中に設けられた鷹梓機、６
は該蝿梓機の駆動モータ、７はこの損梓機の回転数を表
示するタコメータ、８はモータの電源、９は該モータの
回転数を調節する装置、１川まこの回転数調節装置を構
成している可変抵抗器を示す。本発明装置（図中、破線
Ａによって取囲んだ部分）は、培養液内の酸素濃度を検
出しその酸素濃度値が或る設定された基準酸素濃度値か
ら上または下にずれた場合にその偏差を表示する偏差表
示装置１１と、この酸素濃度の偏差に応じて、この偏差
を零とする方向に上記のモー夕６の回転数を増加または
減少させるために該可変抵抗器１０の抵抗値を増加また
は減少させる可逆モータ１２をいずれかの方向に回転さ
せるモータ回転方向切襖装置１３と、上記の偏差値の大
きさに応じて該可逆モータ１２の回転速度を増減させる
モータ回転速度制御装置１４とを備えたことを特徴とす
る。Embodiments of the present invention shown in the drawings will be described below.
In the figure, 1 is a culture tank containing a culture solution, 2 is a culture solution contained therein, 3 is a detector that detects the dissolved oxygen concentration in the culture solution, and 4 is the oxygen concentration detected by the detector. Dissolved oxygen meter that displays 5, Takaazusa machine installed in the culture tank, 6
7 is a tachometer for displaying the rotation speed of the fly azusa machine, 8 is a power source for the motor, 9 is a device for adjusting the rotation speed of the motor, and 1 is a rotation speed adjustment device for Makoto Kawa. The configured variable resistors are shown. The device of the present invention (the part surrounded by the broken line A in the figure) detects the oxygen concentration in the culture solution and detects the oxygen concentration when the oxygen concentration value deviates above or below a certain set reference oxygen concentration value. A deviation display device 11 that displays the deviation, and a resistance value of the variable resistor 10 to increase or decrease the rotational speed of the motor 6 in the direction of zeroing this deviation according to the deviation of the oxygen concentration. a motor rotation direction switching device 13 that rotates the reversible motor 12 in either direction to increase or decrease the rotational speed of the reversible motor 12, and a motor rotation speed control device that increases or decreases the rotation speed of the reversible motor 12 according to the magnitude of the deviation value. 14.
上記の偏差表示装置１１は、上記の溶存酸素計４によっ
て指示された酸素濃度を表わす電圧を前層増幅器１５を
介して比較器１６に印加すると共に基準電圧設定回路１
７によって発生された基準酸素濃度値をあらわす基準電
圧を該比較器１６に印力０して、その偏差をメーターリ
レー１８に加え０るように構成されている。モータ回転
方向切換装置１３は、該メーターリレー１８によって制
御される接点１９を有し、酸素濃度値が設定基準値から
上または下にずれた場合にその偏差に応じて接点１９を
作動して可逆モータ１２をいずれかの方タ向に回転させ
、上記の酸素濃度の偏差を零とする方向にモータ６の回
転数を増加または減少させるように上記の可変抵抗器１
０の抵抗値を増減する。モータ回転速度制御装置１４は
、比較器１６によって表示される偏差値の大きさに応じ
て、該可逆モータ１２の回転速度を増減させ、すなわち
、偏差が大きい時にはモータを高速で回転させ、また偏
差がづ・さし、時にはモータを低速で回転させるように
、モ−夕の回転速度を制御するように構成されていて、
該比較器から偏差に相当する電圧を受けるＶ／Ｆ変換器
２０およびこれに接続されたモータ駆動回路２１を含み
、この回路２１によってモータ１９を所望の速度で回転
する。なお、モータ６の回転数の調節によって酸素濃度
を調節しても、酸素の溶解、検出器の反応速度に遅れが
あるために、酸素濃度の変化の表示に或る程度の遅れが
生ずるので、このような遅れを補償するために、モータ
６の回転数の調節の際にその増速または減速を或る一定
期間やめて実際の酸素濃度の表示の変化を待つためのイ
ンタラプタ２２が設けられる。このようなインタラプ夕
２２の構成自体は既知のもので、本発明の要旨には無関
係である。上述のように、本発明装置は、培養液の溶存
酸素濃度が或る設定された基準酸素濃度値からずれた場
合に、その偏差の方向および大きさに応じて、可逆モー
タを望の方向に適当な回転速度で回転して、酸素濃度値
を速やかに基準値に戻すように働く。The above deviation display device 11 applies a voltage representing the oxygen concentration indicated by the above dissolved oxygen meter 4 to the comparator 16 via the front layer amplifier 15, and also applies the voltage representing the oxygen concentration indicated by the above dissolved oxygen meter 4 to the reference voltage setting circuit 1.
The reference voltage representing the reference oxygen concentration value generated by the sensor 7 is applied to the comparator 16, and the deviation is applied to the meter relay 18. The motor rotation direction switching device 13 has a contact 19 that is controlled by the meter relay 18, and when the oxygen concentration value deviates above or below from the set reference value, the motor rotation direction switching device 13 operates the contact 19 according to the deviation to make it reversible. The variable resistor 1 is configured to rotate the motor 12 in either direction and increase or decrease the rotational speed of the motor 6 in a direction that makes the deviation in oxygen concentration zero.
Increase or decrease the resistance value of 0. The motor rotation speed control device 14 increases or decreases the rotation speed of the reversible motor 12 according to the magnitude of the deviation value displayed by the comparator 16. That is, when the deviation is large, the motor rotates at high speed, and when the deviation It is configured to control the rotational speed of the motor so that the motor sometimes rotates at a low speed.
It includes a V/F converter 20 which receives a voltage corresponding to the deviation from the comparator and a motor drive circuit 21 connected thereto, which rotates the motor 19 at a desired speed. Note that even if the oxygen concentration is adjusted by adjusting the rotation speed of the motor 6, there is a delay in the dissolution of oxygen and the reaction speed of the detector, so there will be a certain delay in the display of changes in the oxygen concentration. In order to compensate for such a delay, an interrupter 22 is provided for stopping speed increase or deceleration for a certain period of time when adjusting the rotation speed of the motor 6 and waiting for a change in the actual oxygen concentration display. The structure of the interrupter 22 itself is known and is not related to the gist of the present invention. As described above, the device of the present invention operates the reversible motor in a desired direction depending on the direction and magnitude of the deviation when the dissolved oxygen concentration of the culture solution deviates from a certain set standard oxygen concentration value. It rotates at an appropriate rotation speed and works to quickly return the oxygen concentration value to the standard value.
この装置は、培養液の溶存酸素濃度を常に監視して、こ
の溶存酸素濃度の変化が生じた時に、これを直ちに設定
基準値に戻すように働き、有効に且つ精密に酸素濃度を
調節することを可能にする。図面の簡単な説明図面は本
発明装置の一実施例を示す概略図である。This device constantly monitors the dissolved oxygen concentration of the culture medium, and when a change in dissolved oxygen concentration occurs, it immediately returns it to the set standard value, thereby effectively and precisely adjusting the oxygen concentration. enable. BRIEF DESCRIPTION OF THE DRAWINGS The drawing is a schematic diagram showing one embodiment of the device of the present invention.
１・・・・・・培養槽、２・・・・・・培養液、３・・
・・・・検出器、４…・・・港存酸素計、５・…・・燈
伴機、６・・・・・・駆動モータ、７・・・・・・タコ
メータ、８・…・・電源、９・・・・・・モータ回転数
調節装置、１０・・・・・・可変抵抗器、１１・・・・
・・偏差表示装置、１２・・・・・・可逆モータ、１３
・・・・・・モータ回転方向切換装置、１４・・・・・
・モータ回転速度制御装置、１５・・…・前層増幅器、
１６・・・・・・比較器「 １７・・・・・・基準電圧
設定回路、１８・・・・・・メーターリレー、１９・・
・・・・接点、２０・…・・Ｖ／Ｆ変換器、２１…・・
・モータ駆動回路、２２・・・ィンタラプタ。1...Culture tank, 2...Culture solution, 3...
...Detector, 4...Port oxygen meter, 5...Lamp, 6...Drive motor, 7...Tachometer, 8... Power supply, 9...Motor rotation speed adjustment device, 10...Variable resistor, 11...
... Deviation display device, 12 ... Reversible motor, 13
...Motor rotation direction switching device, 14...
・Motor rotation speed control device, 15...front layer amplifier,
16...Comparator 17...Reference voltage setting circuit, 18...Meter relay, 19...
...Contact, 20...V/F converter, 21...
- Motor drive circuit, 22...interrupter.
Claims (1)
液中へ流加される基質の流加速度の調節等によつて培養
液の溶存酸素濃度を制御するに当り、上記の撹拌機の駆
動モータ或いは基質給送モータ等の溶存酸素濃度を制御
するモータの回転数を調節する手段を設けて、該手段の
調節により培養液の溶存酸素濃度を制御する装置に於て
、 培養液内の酸素濃度を検出しその酸素濃度値が或る
設定された基準酸素濃度値から上または下にずれた場合
にその偏差を表示する偏差表示装置と、 この検出され
た酸素濃度の偏差に応じて、この偏差を零とする方向に
、上記の撹拌機の駆動モータまたは基質給送モータ等の
溶存酸素濃度を制御するモータの回転数を増加または減
少させるために、上記の手段たる可変抵抗器の抵抗値を
増加または減少する可逆モータをいずれかの方向に回転
させるモータ回転方向切換装置と、 上記の偏差値の大
きさに応じて該可逆モータの回転速度を増減させるモー
タ回転速度制御装置とを備え、溶存酸素濃度を平均的に
制御することを特徴とする培養液の溶存酸素濃度を制御
する装置。1. When controlling the dissolved oxygen concentration of the culture solution by adjusting the rotation speed of the stirrer that stirs the culture solution or adjusting the flow rate of the substrate added into the culture solution, the above-mentioned drive of the stirrer In an apparatus that is provided with a means for adjusting the rotation speed of a motor, such as a motor or a substrate feeding motor, for controlling the dissolved oxygen concentration, and controls the dissolved oxygen concentration of the culture solution by adjusting the means, a deviation display device that detects the oxygen concentration and displays the deviation when the oxygen concentration value deviates above or below a preset reference oxygen concentration value; In order to increase or decrease the rotation speed of the motor that controls the dissolved oxygen concentration, such as the drive motor of the stirrer or the substrate feeding motor, in the direction of zeroing the deviation, the resistance value of the variable resistor, which is the means described above, is determined. a motor rotation direction switching device that rotates a reversible motor in either direction to increase or decrease the deviation value, and a motor rotation speed control device that increases or decreases the rotation speed of the reversible motor according to the magnitude of the deviation value, A device for controlling the dissolved oxygen concentration of a culture solution, characterized by controlling the dissolved oxygen concentration on an average.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP13353277A JPS6022907B2 (en) | 1977-11-09 | 1977-11-09 | Device that controls dissolved oxygen concentration in culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP13353277A JPS6022907B2 (en) | 1977-11-09 | 1977-11-09 | Device that controls dissolved oxygen concentration in culture medium |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5467085A JPS5467085A (en) | 1979-05-30 |
| JPS6022907B2 true JPS6022907B2 (en) | 1985-06-04 |
Family
ID=15106993
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP13353277A Expired JPS6022907B2 (en) | 1977-11-09 | 1977-11-09 | Device that controls dissolved oxygen concentration in culture medium |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6022907B2 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6018390B2 (en) * | 1981-01-21 | 1985-05-10 | 株式会社日立製作所 | Aerobic culture control method for microorganisms |
-
1977
- 1977-11-09 JP JP13353277A patent/JPS6022907B2/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5467085A (en) | 1979-05-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4234007A (en) | Automatic liquid flow control device | |
| KR830001014A (en) | Automatic preset welding system | |
| US4991502A (en) | Damping system for an offset press | |
| JPS6022907B2 (en) | Device that controls dissolved oxygen concentration in culture medium | |
| GB1222484A (en) | Chlorine residual controlling system | |
| GB1523318A (en) | Controlling the depth of immersion of the electrode in an electro-slag remelting furnace | |
| JPH0368746B2 (en) | ||
| MX9606123A (en) | Apparatus for automatic production of ice. | |
| KR870004559A (en) | Induction Motor Control System and Method | |
| JPH02203139A (en) | Ventilation fan | |
| JPS5820456A (en) | Ink density management method | |
| CN206884018U (en) | One kind stream film thickness automaton | |
| JPS5823073B2 (en) | How to adjust dissolved oxygen | |
| JP2523821B2 (en) | Raw material supply device for CZ furnace | |
| JPH07114810B2 (en) | Infusion pump drive motor control method | |
| JP3762848B2 (en) | Bank amount control method in roller head extruder | |
| JPH0764383B2 (en) | Preset liquid supply device | |
| US4028605A (en) | System for passing products to be treated through a machine | |
| JP2726373B2 (en) | Yeast automatic addition device | |
| JPS6448763A (en) | Tension adjusting device in tape feed mechanism | |
| JPS6243206B2 (en) | ||
| SU730460A1 (en) | Apparatus for automatic starting and regulating of liquid metal level in mould of machine for continuous steel casting | |
| JPS62168652A (en) | Molten metal surface level control method in continuous casting machine | |
| JPH0149938B2 (en) | ||
| JPS5538905A (en) | Fixed quantity feeding method for molten slag to slag spout |