JPS6023832B2 - Protozoa and micrometazoa culture equipment - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a culture device that can effectively breed protozoa and micrometazoans by providing food in an easily ingestible state.

Protozoa and micrometazoa eat rice bran, using bacteria, yeast, actinomycetes, algae, mold, etc. as food.

Therefore, in order to culture these animals, there are two methods: one is to simultaneously culture food in an animal culture tank, and the other is to provide a separate food culture tank. However, in either method, if the food tends to aggregate and the floc size becomes larger than the cell mouth of protozoa or the mouth of micrometazoa, it becomes difficult to feed, and the specific growth rate of these animals decreases. concentration decreases. SUMMARY OF THE INVENTION An object of the present invention is to eliminate the drawbacks of the prior art and provide an apparatus for stably culturing protozoa and micrometazoans at high concentrations. In this specification, micrometazoans refer to micrometazoans having a size of a few ribs or less, such as sacs, annelids, and arthropods.

According to the present invention, the above object is achieved by providing a means for reducing the floc size of the feed by 4 mm from the animal's mouth, that is, a micronization tank between the feed culture or adjustment tank and the animal culture tank. Ru.

The fine material used in the present invention may be any sieve having a device capable of applying a breaking force to the culture solution containing food, such as a mechanical flocculation device or an ultrasonic generator.

It is also advantageous to provide a baffle plate in the mill to facilitate finer flocs. Next, the present invention will be explained in detail based on the drawings.

FIG. 1 is a system diagram of the apparatus of the present invention.

In FIG. 1, a bait culture or adjustment tank 1 includes bacteria, yeast,
This is an adjustment tank that stores sludge for cultivating food such as actinomycetes, algae, and molds, surplus sludge from biological treatment of wastewater, and primary sludge of wastewater. The cultured or adjusted bait is placed in the micronization tank 2.
There, they are pulverized by ultrasonic treatment, mechanical simulation, etc., and made into a size that is easy for animals to eat. The size of the cytostomes of protozoa and the mouths of microzoans and metazoans vary significantly depending on the type of these animals, with the largest being 500 mm and 4 mm.
・There are cuttings of 50# or less. Therefore, the upper limit of the size of the bait differs depending on the type of protozoa or micrometazoan cultured in the animal culture tank 3, and the conditions for refining the bait differ. The strength of the rope pestle should be selected appropriately, taking into consideration these conditions and the floc size of the bait. 2 and 3 show a refining tank consisting of a turbine type fly punch tank 4. FIG.

A baffle plate 6 is provided on the wall of this stem tank, and a flat blade 5 is provided in the center of the mulch. The bait in the tank is crushed by the rotation of the flat blades 5, and further refined by colliding with the baffle plate 6. The drawing shows an example in which a turbine-type tomo-azusa machine is provided, but the fly-azusa machine may also be a sardine-type sardine machine, a sardine-type sardine machine, a propeller-type sardine machine, etc., which give deep cutting force to the bait. Any device that can be used for miniaturization is sufficient.

By passing the food through such a miniaturized tank, the food is always provided in a size that is easily ingested by the animals, so animals can be cultured at a stable high concentration without reducing the specific growth rate of the animals. be able to.

Next, the present invention will be described in detail based on Examples, but the present invention is not limited thereto.

Example 1 A medium containing beptone and meat extract as the main ingredient
vocterium), ultrasonication is carried out in a micronization tank, the food is reduced to less than 5, and this food is used to
meciyama m) was cultured.

The operating conditions for each pot are as follows: Bait culture tank: Residence time: 6 hours; Temperature: 2000℃ dissolved oxygen 8
．． 902 of 8/Sea bait micronization tank: Residence time, 10 minutes Ultrasonic treatment: Magnetostrictive microhorn 20KHz, 50W Elephant insect culture tank: Residence time, 1 unique time, temperature 20oo
, dissolved oxygen8. &902' During the ultrasonic treatment, the generation of hydrogen peroxide was investigated using a colorimetric method using titanium sulfate, and the concentration was 2.5 per oxide in 10 minutes, which had no effect on living organisms.

For comparison, culture was carried out under the same conditions as above (conventional two-pot method) except that the fine charcoal was omitted.

In the case of the method of the present invention, feed could be supplied in a quantity of 5 μm or less, and the parasitic insects could be continuously cultured at a concentration of 1 × 1 insect/cm. On the other hand, with the conventional method, the bait flocculated to a maximum of about 500 larvae, and the concentration of weevil insects decreased to 1 × 1 insect/ship. Example 2 Micrococcus (Micr-like occ female Varia
nsATCC399) was continuously cultured in a culture tank, mechanically illuminated in a micro-cell, feed was reduced to 60 mounds or less, and Vemcella was cultured in an animal culture tank.

The hawk frame was made using a turbine-type tomazusa machine shown in Figures 2 and 3. The operating conditions for each dan are as follows. Feeding doubling: Residence time, 3 hours Temperature 2000 Dissolved oxygen 8.8Moo2'〆Feed fine ○ tube: Residence time, 1 hour 120 pm Culture tank for turret bugs: Residence time, 1 hour temperature 20○ Dissolved oxygen 8. &902/k Under the same conditions as above except that the atomization tank was omitted (conventional 2
(tank type) culture was performed.

According to the present invention, bait can be prepared in less than 60 piles, and
It was possible to continuously culture worms at a concentration of 1 x 1 insect/secretion.

On the other hand, in the conventional method, the bait aggregated to a maximum of about 300 ml, and the concentration of worms decreased to 1 x 10 insects/me. As described above, according to the present invention, protozoa and micrometazoans can be stably cultured at high concentrations.

[Brief explanation of drawings]

FIG. 1 is a system diagram of the culture apparatus of the present invention, FIG. 2 is a plan view of a turbine-type fly bean tank, and FIG. 3 is a schematic sectional view of the turbine-type fly bean tank shown in FIG. 2. Explanation of symbols, 1...Food culture or adjustment tank, 2.
...Minimization tank, 3...Animal culture tank, 4...
...Turbine type fly azusa tank, 5...Flat blade, 6.
...Baffle board. Figure 1 Figure 2 Figure 3

Claims (1)

[Scope of Claims] 1. A tank for culturing or preparing food such as bacteria, yeast, actinomycetes, algae, molds, etc. that protozoa and micrometazoa feed on, and a tank for culturing or preparing food such as bacteria, yeast, actinomycetes, algae, and molds that protozoa and micrometazoa feed on, and A culture device for protozoa and micrometazoans, characterized in that a bait atomization tank is provided between a tank for breeding metazoa. 2. The culture device according to claim 1, wherein the bait atomization tank is a tank equipped with an ultrasonic generator or a mechanical stirring device.