JPS6024075B2 - Method for manufacturing organic fertilizer ripening agent - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention involves producing a ripening agent using thermophilic fibrinolytic bacteria and purple non-sulfur bacteria, which play a major role in the ripening of organic fertilizers, and dispersing them. The present invention relates to a method for facilitating propagation and achieving rapid and reliable ripening of organic fertilizers.

After the war, the democratization of political and social systems and the transformation of the industrial structure leveraging technological innovation had a significant impact on agriculture and related production industries, leading to the spread of complex agricultural machinery, energy consumption, systemization, and chemical innovation. A simple farming method that focuses less on fertilizers and pesticides and aims to increase yields.

This is the so-called modern farming method, and the result is that today, after the third post-war king, agricultural crops and vegetables are attacked by pests and diseases, combined with natural phenomena such as abnormal weather, yields are decreasing, quality is deteriorating, and agricultural production is Most of Japan's cultivated soil, which is the foundation of rice cultivation, has been physically, chemically, and microbiologically destroyed and is becoming degraded soil or dilapidated paddy fields. A strong reflection on this reality has led to stronger promotion of the ``Skill-building Movement'' by the competent authorities and other related organizations. In addition, farmers themselves have devised various methods such as natural farming, organic farming, enzyme farming, algituto farming, and Hunza farming to increase soil fertility by applying organic fertilizers. But it hasn't achieved much success.

One of the major reasons for this is that farmers no longer have large livestock such as horses and cows, even if they are simply producing Thai or Kyu manure. Organic fertilizers are fertilizers with a carbon content of 50 to 100, such as rice straw, wheat straw, fallen leaves, bark, and sawdust.
It can be thought of as Thai fertilizer or cucumber fertilizer made mainly from the above plant remains.

As described above, the plant remains are piled up, usually aerated, and rotten at high temperatures.

``One of the main bacteria that plays an active role in the ripening of organic fertilizers is a thermophilic fibrinolytic bacterium, which generally lives in the soil, but is very often derived from large livestock feces.Therefore, the purpose of the present invention is to The aim is to cultivate this thermophilic fibrinolytic bacterium, spread it as a seed, and propagate it to more reliably and more promote the ripening of organic fertilizer. Immature, which has already been fertilized on the farmland by spraying ripening agents.
Another object of the present invention is to produce an extremely excellent ripening agent for organic fertilizers that can also promote the maturation of semi-ripened organic fertilizers. The composition of the present invention consists of a culture of thermophilic fibrinolytic bacteria and purple non-sulfur bacteria mixed with calcium carbonate mixed with an organic nitrogen source for microorganisms involved in the ripening of organic fertilizers and a nutrient source for growth factors. A method for producing a ripening agent for organic fertilizer, which is characterized in that it is made into granules using an excipient mainly consisting of powder.

In the present invention, thermophilic fibrinolytic bacteria and purple non-sulfur bacteria were selected and cultured, and these cultures were mixed at the same time.The reason is that thermophilic fibrinolytic bacteria are the main actors in the ripening of organic fertilizers. The purple non-sulfur bacteria actively assimilate harmful organic low-molecular compounds and hydrogen sulfide, which are the metabolic products of this thermophilic fibrinolytic bacterium, and furthermore, depending on the conditions, they can absorb Fixes nitrogen. The coexistence of these two types of bacteria clearly shows a tendency for cellulose decomposition and nitrogen fixation to increase. Therefore, the cooperative action of amphibious bacteria further ensures the rapid and natural ripening process of organic fertilizers (the mechanism of ripening of the fertilizer of the present invention is explained on pages 14 to 2).
(Details will be explained in detail by the swordsman). Hereinafter, the present invention will be explained in detail. 1 Culture of thermophilic fibrinolytic bacteria Microorganisms that decompose fibrillum include aerobic, anaerobic, and thermophilic, and are morphologically MiCro-CoC.
Cucumber, BaCilliS, BaCteri mountain
, Crostridium, PseMomonas,
There are bacteria such as Spimchaeta and actinomycetes Actinomycetes.
Many species belonging to the genus Inomyces have been discovered.

In addition, methane bacteria that physiologically decompose cellulose and generate particularly large amounts of methane gas, hydrogen bacteria that generate hydrogen, and those that have nitrate-reducing properties are also included in this category. Furthermore, in acidic and aerobic conditions, decomposition by filamentous fungi is also considered to occur. However, thermophilic bacteria play an important role in the decomposition and ripening of organic fertilizers due to their high ability to decompose fibre, and their wide range of breeding conditions.

Now, thermophile is described as "thermophile" in the academic glossary of the Ministry of Education, and the translated term is "heat-resistant bacteria," but it is true to its English name that it is thermophile. Bacteria.

Also, a similar term is thermophilic bacteria (heron cult).
ative ennophile), obligate thermophilic bacteria or obligate thermophilic bacteria (strict or obliga
thermophile), highly thermophilic bacteria (reme thennophile), and moderately thermophilic bacteria (mmerate thermophile), and there are various academic disagreements about their classification, but in the present invention, thermophilic bacteria The appropriate temperature is 55 ko 0 or 55
Bacteria that grow above qo are determined. Therefore, the thermophilic fibrinolytic bacterium employed in the present invention is Clostridium thermocellum
mm), Bacillus thermosellolithecus
Bacillus thermofibrincolus, Bacillus cellulolyticus, Bacillus cellulolyticus
solvens) type bacteria and their closely related strains and associated bacteria. Further, in the present invention, actinomycetes of the genus Thermoactinomyces are always included as accompanying bacteria. The optimum temperature for the growth of the bacteria used in the present invention is between 55:00 and 65:00, and the upper limit may exceed 75:00. The lower limit is usually 40 o'clock, and it will not grow below 40 o'clock. It makes good use of organic nitrogen, actively ferments cellulose as a carbon source, and completes the fermentation in 48 to 60 hours. Others ferment glycerin, xylose, arabinose, glucose, cellobiose, sucrose, lactose, maltose, mannitol, inulin, starch, and hemicellulose. The fermentation rate of cellulose varies depending on the bacterial strain, but it contains 60-85% of the cellulose tested, ethanol, acetic acid, lactic acid, formic acid, lactate, cellobiose, glucose, carbon dioxide, hydrogen, indole, hydrogen sulfide, etc. to produce.

The optimum pH for growth is 6.8-7.8, and the redox potential is Eh.
It is estimated to be between -200 and -35 skin V.

Thermophilic fibrinolytic bacteria are originally anaerobic bacteria, but
It always coexists with thermophilic aerobic bacteria as companion bacteria. However, the relationship between these two bacteria is still unclear.
Thermophilic fibrinolytic bacteria have been isolated, and no research has been found on pure bacteria. Furthermore, the above results are the results of laboratory research using glass equipment on pure cellulose such as paper leakage, pulp, and absorbent cotton, and the cellulose concentration tested was around 1%. Moreover, unless special measures are taken such as a continuous fermentation method in which fresh tower liquor and fermentation liquor are constantly exchanged, the overall decomposition of cellulose remains at less than 1%. in addition,
Thai manure has a remarkable decomposition power in the natural world, such as in the soil, which has cellulose as its main component, such as ash and wood chips, and it also has a good ability to actively decompose pure cellulose in paper leakage, absorbent cotton, etc. Pyrogenic fibrinolytic bacteria alone are incapable of decomposing natural fibrous substances at all, even in rigorous laboratory experiments. Thermophilic fibrinolytic bacteria were cultured using the medium of Vjlioen, Fred, Pete Gon [
1926]: 5 nights of bepton, excess calcium carbonate, 2 nights of sodium ammonium hydrogen phosphate, 1 night of potassium dihydrogen phosphate, 0.3 nights of magnesium sulfate, 1 night of calcium chloride, traces of ferric chloride, 15 nights of cellulose , well water 1000c
Use c.

Or a part thereof is replaced with a natural product and cultured at 60±5°C under anaerobic or semi-anaerobic conditions for 48 to 6 hours. 2. Cultivation of purple sulfur-free bacteria Photosynthetic bacteria are broadly divided into three families: purple sulfur bacteria, green sulfur bacteria, and purple sulfur-free bacteria.

The bacteria used in the present invention are purple non-sulfur bacteria (Athior
Rhodomonas (Rhodop)
seudomonas) and Rhodospirillum (Rhod
ospirjllmm) belongs to this category.

This bacterium is considered to be a family of facultative anaerobic bacteria, and grows under any of the following conditions: anaerobic/light, aerobic/light, and aerobic/straight. Organic low-molecular compounds such as lower fatty acids such as acetic acid, propionic acid, butyric acid, and lactic acid, ethanol, glycerol, mannitol, sorbitol, amino acids, and monosaccharides and oligosaccharides such as glucose and fructose are preferably used. In this case, in anaerobic and light conditions, the plants grow by using the energy of light to fix carbon dioxide and fix nitrogen using hydrogen sulfide, sulfides such as sodium thiosulfate, hydrogen, or organic compounds as hydrogen donors. Another characteristic of purple nonsulfur bacteria is that, like other photosynthetic bacteria, they have a photosynthetic organ chromatophore (
chromatophore).

It contains bacteriochlorophyll a and carotenoid pigments, and carries out photosynthesis. These pigments are easily separated and purified, and their presence brings favorable results not only to the growth of bacteria and other microorganisms, but also to the growth of higher plants. H blood r [
1946] medium: dipotassium hydrogen phosphate 0.05%,
Potassium dihydrogen phosphate 0.05%, diammonium hydrogen phosphate 0.08%, magnesium sulfate 0.02%, lactic acid 0.3%, acetic acid 0.1%, citric acid 0.1%, Fe20
0y%, Ca500y%, B5y%, Culy%, Mm
100y%, Zn200y%, Ga1y%, biotin 1
3.7 Buddha dinner, yeast autolysate 600/9, distilled water loo
0 is basically base material, and depending on the situation at the time, some parts are substituted with natural products.

Culture at 25±7° C. under aerobic or anaerobic, bright (light) or dim light conditions for 48 to 7 hours.

3 Addition of organic nitrogen sources and growth factors It has already been clarified by the research of the present inventors that organic nitrogen sources such as beptone are necessary for the growth of thermophilic fibrinolytic bacteria. However, subsequent research revealed that
Since it was found that these compounds are relatively low-grade nitrogen-containing compounds, 0.01 to 0.05% of animal protein decomposition products containing these compounds are added to the present invention.

In addition, both thermophilic fibrinolytic bacteria and purple nonsulfur bacteria require specific growth factors. If these are missing, continuous culture of thermophilic fibrinolytic bacteria becomes difficult, and growth of purple nonsulfur bacteria stops and abnormal fermentation occurs. Therefore, the growth factor for the former is UGF-Q, and the growth factor for the latter is UGF-8 (also known as Gloster). 4. Creation of chin grains using excipients Concentrated bacterial body fluid obtained by adding a suitable natural polymer flocculant to the above-mentioned cultures of thermophilic fibrinolytic bacteria and purple non-sulfur bacteria, an organic nitrogen source, The growth factors and the like are added to an excipient mainly composed of calcium carbonate powder, mixed well, and made into neck granules with a diameter of about 2 to 8 ribs using a verit machine.

An example of the combination of raw materials is as follows. Mixing ratio of raw materials (for 1000 terres of calcium carbonate powder)○
Concentrated bacterial body fluid of thermophilic fibrinolytic bacteria...0.1 evening○Dense bacterial body fluid of purple non-sulfur bacteria...0.4 evening○Organic nitrogen source solution...1.0 evening.

UGF-Q...3 of 50
OUGF 18 (also known as Gloucester)...10 female 0 Rutin...10 pairs 90 tunagi agent...50 yen Calcium carbonate powder...1000 ta In this way, 3
Over the years, the eerie advances in new chemical agriculture, such as chemical fertilizers and pesticides, have finally driven Shizuo into a world of death, a barren world with an imbalance of microorganisms, and moreover, farmers have almost no large livestock. At present, the following advantages can be listed as excellent effects of the ripening agent of the present invention. ■ The selection of thermophilic fibrinolytic bacteria and purple non-sulfur bacteria, and the skillful cooperative relationship between these two bacteria, is not simply a synergistic effect.

This relationship will be explained from a microbial ecological standpoint. In addition, Example 1 demonstrated the excellent cooperation and synergistic effects of both bacteria in combination in fields such as paddy fields, fields, and orchards.
Further details are shown in Sections 5 to 5. In the case of microorganisms, ecologcal succession similar to that in plants is observed.

However, there are also fundamental differences between the two. In other words, in plants, the transition progresses in the direction of production and reaches the peak phase, but in the case of microorganisms, the transition progresses in the direction of reaction and decomposition. This phenomenon is due to the fundamentally different roles of the two in the ecosystem, with the former being a producer and the latter being a decomposer. The transition of microorganisms takes place during this decomposition process, and the peak phase for microorganisms is a state in which organic matter has been completely decomposed and microbial activity has completely ceased.

Organic matter that is decomposed by microorganisms is generally plant stems, leaves, trees, etc.
Such as tree bark.

Furthermore, there are differences in the decomposition process between woody plants and herbaceous plants, but in any case, components that are easily decomposed are used in the order of priority. In this way, during the transition of microorganisms, the types of microorganisms that emerge differ depending on the components of organic matter, and the types also change depending on changes in the environment.

Most of the organic matter in Thai manure and farmland comes from herbaceous plants. Therefore, the decomposition process of these organic substances can be considered to represent the transition of microorganisms that decompose herbaceous plant remains. Therefore, as an example for explaining the present invention, the decomposition of herbaceous plants in agricultural land will be described.

However, in agricultural land, humans have added cultivation, fertilizer, and fertilizer to the natural ecosystem.
They are special ecosystems whose stability is maintained by inputs of energy in the form of pesticides. In order to increase net production in this agro-ecosystem, fertilizers are constantly applied and tillage is applied to increase microbial activity.

The cycle of agricultural ecosystems is characterized by the constant movement of substances from producers to primary and secondary consumers, and the agricultural products that are producers are taken out of the system as harvests. Therefore, humans must continuously manage cultivated crops artificially. These are the following four technologies of modern agriculture: mechanization,
Fertilization, pesticides (chemical control of weeds and insects)
It has brought great progress to the world, but at the same time it has disrupted the complicated cycle of biological gardens. For example, the application of pesticides not only kills weeds and pests, but also allows the pesticides to penetrate into the soil. This permeated pesticide also kills or reduces microorganisms in the soil. In other words, even if you spray pesticides for the purpose of exterminating pests, it will not only kill the target pests, but also other insects and microorganisms.

Now, regarding the decomposition of herbaceous plant remains, which is the subject of this paper, a large number of microorganisms are at work.

However, these microorganisms do not work separately, but are closely related to each other, and microorganisms with various properties and functions appear and disappear in a regular manner. Also, certain groups of microorganisms appear one after another. When the activity of the microorganisms of the previous period begins to decline, the microorganisms of the next period begin to be active. Microbial activity in one period sets the stage for microbial activity in the next period. In this way, how microorganisms take turns,
The appearance of microbial succession will be described according to changes in plant components.
The main organic component of herbaceous plant remains is cellulose, which accounts for 25-35%
The carbon content is around 50 to 70, followed by lignin, protein, bran, and others in that order.

When such organic materials are piled up and decomposed and ripened, the microorganisms that appear are broadly classified into the following three stages, and decomposition by microorganisms proceeds in this order. 1st stage Decomposition of sugars and proteins (decomposition of pectin, hemicellulose) Bacteria, filamentous fungi (actinomycetes) 2nd stage Decomposition of fibrillum Thermophilic fibrinolytic bacteria, aerobic and anaerobic bacteria, actinomycetes, filamentous fungi 3 Stages Lignin Decomposition Fungi (Mushrooms) Stage 1: The microorganisms that emerge are mainly bacteria and filamentous fungi, which rapidly decompose easily assimilated sugars, inulin, starch, amino acids, proteins, etc.

They multiply rapidly and break down these nutritional substances. As soon as it is exhausted, it disappears. Mau.

At the same time, as the environment changes, they form countless spores, create durable bodies, and go dormant.

There are countless microorganisms in nature that are involved in this first step. Because there are so many varieties, there is competition among farmers. intense.

Pectin and hemicellulose are free In this state, sugars, proteins, etc. It disassembles quickly in the same way, but What binds to lurose and gunin 4 Decomposition is slightly delayed.

Second stage: Next, thermophilic fibrinolytic bacteria appear.

During the pre-process, bacteria and filamentous fungi grow extremely quickly and become active, consuming oxygen and generating a lot of heat.

This fever gradually accumulates until 60~ Because the temperature is close to 70 qo, Anaerobic thermophilic fibrinolytic bacteria work The conditions are in place.

On the other hand, bacteria and filamentous substances that assimilate basal substances and proteins Bacteria grow due to their own heat. growth is suppressed, and growth is suppressed. Sugars, which are nutrients, also consume protein. After being exhausted, growth stopped, and finally Giving territory to thermophilic fibrinolytic bacteria That's why I give it to you.

Looking at examples of environmental changes as factors that cause transitions Can be done.

However, this second stage process Even if thermophilic fibrinolytic bacteria act alone, It's not due to wood.

Decomposes cellulose aerobically even for thermophilic microorganisms Decomposes cellulose anaerobically with bacteria that do not The thermophilic cellulose selected in the present invention There is decomposition, but the organic matter in the pile is Both bacteria are closely related to decomposition. Ru.

First, aerobic thermophilic bacteria and Streptomyces work.

These bacteria generally do not have the ability to decompose fibrin, but
Decomposes hemicellulose and pectin.

The cellulose that makes up plant cell walls is bound together by a shell of hemicellulose, pectin, lignin, etc., so unless some of these shells collapse, the cellulose present inside will not be decomposed.

Therefore, this kind of cooperation by aerobic thermophilic bacteria,
If lime nitrogen or quicklime is added to dissolve part of the outer shell of hemicellulose and lignin, the decomposition of cellulose will proceed smoothly.

In this way, aerobic thermophilic bacteria help decompose fibers by stripping them of cellulose, and at the same time actively consume oxygen, creating an oxygen-deficient environment around themselves.

Anaerobic thermophilic fibrinolytic bacteria are active there. This is how we start.

Third stage: After the peak of activity of thermophilic fibrinolytic bacteria passes, the temperature of the tumbler begins to drop.

Actinomycetes often emerge from around this time.

The remaining cellulose is removed relatively slowly, but by mesophilic Anaerobic or aerobic fibrinolysis Continues to decompose together with bacteria.

Next, microorganisms decompose lignin. The transition advances to the third stage.

Lignin is a hemiacetic compound in plant cell walls. Exists in combination with ululose, cellulose, etc. present and easily degraded by microorganisms Not possible.

When hemicellulose and cellulose are decomposed and released from their bonds, lignin undergoes some decomposition, albeit very slowly, to become altered lignin.

This altered lignin is derived from microbial cells. Combines with proteins to form a black complex Become.

Has the ability to break down lignin Mainly fungi (mushrooms) ).

Therefore, if mushrooms start to grow, it can be concluded that the decomposition of organic matter has progressed to almost the final stage.

Decomposition of lignin through the action of mushrooms The organic matter that should be assimilated after being It's almost gone, leaving a black residue. Only the investigation remains.

This residue is called rot.

However, septic is not only the residue, but also the soil Small animal artifacts and microorganisms mentioned above Complexes combined with protein are also serious. So, these things are intricately connected. It is something.

As mentioned above, the method of decomposing plant remains is to break down the components that are easier to decompose in the order of sugar and proteolytic bacteria → (thermophilic) fibrinolytic bacteria → lignin-degrading bacteria.

In other words, there is a transition from high productivity to low productivity, and eventually there is no more organic matter to be utilized and microbial activity stops. Therefore, I would like to reiterate my point that in modern agriculture, where chemical fertilizers and pesticides are overemphasized and the microbial ecosystem is out of balance, the application of this ripening agent is important because it is important for the growth of various microorganisms in the chain of succession. It requires cooperation, but
I think you can clearly see that the key is to introduce thermophilic fibrinolytic bacteria, which plays an important role as a central point.

Next, regarding the function of purple non-sulfur bacteria, they first assimilate low-molecular organic compounds such as bran and amino acids and participate in the first transition stage.

In addition, its proliferation supplies microscopic growth factors to many related useful microorganisms, eliminates Escherichia coli, Salmonella typhi, and other pathogenic bacteria, inhibits the growth of harmful filamentous fungi, and facilitates a smooth decomposition process. naturally and quickly. In the second transition stage, harmful substances produced by thermophilic fibrinolytic bacteria (organic acids such as acetic acid and butyric acid, hydrogen sulfide, etc.) are actively decomposed and purified under favorable conditions. , to effectively continue the cellulose decomposition ability.

Moreover, the effect of using purple non-sulfur bacteria in combination with this ripening agent is
This is particularly noticeable when snails such as rice straw and wheat straw are applied directly to fields and paddy fields.

Unlike rice manure, this type of manure is fresh coarse organic matter, so its decomposition causes nitrogen starvation and the production of organic acids, leading to growth problems in the early stages of paddy rice, root rot due to soil reduction, delayed growth, and reduced yields. This causes various problems.
Although these obstacles cannot be eliminated by the sole application of thermophilic fibrinolytic bacteria, the purpose can only be achieved by the combined use of purple non-sulfur bacteria. The reason is that thermophilic fibrinolytic bacteria produce organic acids, ethanol, and glucose by decomposing fibrin, increasing the decomposition rate.
The organic trophic bacteria in Shizhou utilize these products, so the protein synthesis rate increases locally. Therefore, an increase in the fibrinolysis rate results in a shortage of available nitrogen. Therefore, in such cases, the nitrogen-fixing effects of purple non-sulfur bacteria will become evident. In fact, the coexistence of thermophilic fibrinolytic bacteria and purple nonsulfur bacteria clearly shows a tendency to increase fibrillolytic activity and nitrogen fixation activity.
In addition, the fact that purple non-sulfur bacteria grow in paddy soil in coexistence with other organic trophic bacteria, and that they are involved in the decomposition process of organic acids and hydrogen sulfide, has a major role in nitrogen fixation and removal of harmful substances. It is already known that it has the same function.

Other advantages of the present invention include the following. ■ The granular ripening agent created according to the present invention is
The ripening agent retains its effectiveness for several years in locations with relatively good storage conditions, such as low humidity and berths.

■ Calcium carbonate, which is the main ingredient in molding, not only serves as a nutrient source for thermophilic fibrinolytic bacteria and purple non-sulfur bacteria, but also creates favorable environmental conditions such as hydrogen ion concentration, soil aggregate structure, and other conditions. useful for.

In addition, other additives are nutrients necessary for both species,
Since they are all natural products, they are not harmful to agricultural crops, vegetables, etc. (2) Since the ripening agent according to the present invention is a solid substance in the form of granules, it is convenient to spray, store, etc.

Example 1 This example is an experiment for producing Thai fertilizer.

50 kg of the granular ripening agent of the present invention and water are added to 1000 kg of rice wine and allowed to accumulate for about 2 weeks.

Next, ammonium sulfate and lime nitrogen equivalent to 1.0 to 1.5 kg of nitrogen are added, and water is sprinkled moderately to make it into a main pile while lightly basting. Make a switch once on the way. It ferments well and completes in 45 days. The rice straw was easily torn off and showed a carbon content of 21.5. A control sample, which was carried out in parallel with the addition of calcium carbonate granules 50k9 instead of the granular ripening agent of the present invention in the same manner as above, remained half-ripe even after 3 months.

Although the same machine was used in this example and each of the following examples, the granular ripening agent used was prepared according to the blending ratio described on page 13 of this specification. Example 2 Spraying test in paddy fields.

For paddy fields, 80% of the granular ripening agent of the present invention is used as fertilizer per heap.
K9 is mixed with the three elements and sprinkled, plowed in with rice straw, and attached to the rear shield.

For top dressing, etc., add 10k9 of the neck granular ripening agent of the present invention once per melon.
Mix it with chemical fertilizer and spread it. When water management and other aspects were carried out as usual, the yield of brown rice was definitely more than 20% higher than in previous years. The grains of rice are round, full, and have a weight when measured, and are delicious.

In addition, the plant is long and durable. It divides well, has the largest ears, and has many grains, averaging 130 to 145 grains.
Example 3 This is a fruit tree test for mandarin oranges.

Apply the jaw granular ripening agent 50k9 of the present invention to the February starter fertilizer by mixing a small amount of three elements, mainly ammonium sulfate per melon, and plow in together with weeds, rice straw, etc.

After the flowers fall, topdressing and other management should be carried out as usual. The test results were as follows: (1) The fruit had a good color and gloss, and the sweetness was 2 to 3 degrees higher on average. ■Although the grains were not very large, the results were good, resulting in a 25% increase in sales compared to the average year. ■
A rejuvenating phenomenon has been discovered in old crucian carp. Example 4 This is a test for greenhouse cultivation.

100k9 of the similar granular ripening agent of the present invention per IQ before making
It is applied by mixing it with the base organic fertilizer (three months' worth of soil, mainly consisting of wild flowers and fallen leaves) and blended fertilizer.

Disinfection and other fertilizer management were carried out as usual, but the bacterial wilt and board rot of the previous year did not occur at all.
Harvested tomato fruits are fresh and have good color, and 40
The yield increased by more than %, indicating that continuous cropping is possible by using the granular ripening agent of the present invention. Example 5 This is a test mainly for the cooperative relationship between thermophilic fibrinolytic bacteria and purple non-sulfur bacteria by plowing in raw straw and cultivating wheat.

1 Cultivation overview Variety name Azuma Golden Sowing period November 12th Seed amount 25k9 per melon Sowing method Broadcast herbicide November 15th Saturn Baaro emulsion 1000cc
/1 go step pressure Conducted twice on January 10th and February 5th 2. Exam guests
(per 10a) (Note) '1' ward area, 1 ward system■ Paddy harvest October 29th, starter fertilizer, plowing material applied November 5th, plowing November 10th ■ Top dressing March 15th {5 } Is it N for all Motohi districts? ．． 5kg, P2047.5k
9. K207.5kg Top dressing No.1 in each area. 6k9, P2
041.3k9, K200.9k9'6} The raw materials and blending ratio of the ripening agent used in this test are as described on page 13 of the specification.

3. Test results (per 00a) In terms of plant height, number, and length at the time of growth survey, plot E, to which a ripening agent was applied, was the best, followed by plot F.

4 Dynamics of both bacterial species used together as a ripening agent The following table shows the kinetics of thermophilic fibrinolytic bacteria and purple non-sulfur bacteria in this test.

Note) The number of thermophilic fibrinolytic bacteria '1' was measured using the most probable value method (
most probable is r method
)by. Culture temperature 6500. [21 In addition, purple non-sulfur bacteria are measured by dilution double plate method under sunlight.

The abundance of both bacterial species was highest in E area where the ripening agent was applied.
Considering the wheat quality and yield results mentioned in the previous section, it has been proven that both bacterial species grow in a close cooperative relationship without inhibiting each other.

Of course, this test was conducted on the cultivated wheat crop, one area, and one performance, and the measurement of bacterial counts was limited to thermophilic fibrinolytic bacteria and purple non-sulfur bacteria, before the application of this ripening agent and before harvest. This is the average value of the two measurements immediately after.

Claims (1)

[Claims] 1. The optimum temperature for growth is 55° to 65°C, and anaerobic or facultative anaerobic bacteria that do not grow below 40°C include Clostridium thermocellum, Bacillus thermocellulolitex, Bacillus thermofibrincorus, and Bacillus cellulose desol. A culture of one or more types of thermophilic fibrinolytic bacteria selected from Bens-type bacteria and a culture of purple non-sulfur bacteria are combined with a trace amount of organic nitrogen sources of these microorganisms that are involved in the ripening of organic fertilizers. 1. A method for producing a ripening agent for organic fertilizer, which comprises making it into granules using an excipient mainly composed of calcium carbonate powder mixed with nutrients such as growth factors.