JPS6035399B2 - antibacterial detergent - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a novel detergent having excellent antibacterial properties such as bactericidal and antifungal properties.

Mold and bacteria, which can cause asthma, food poisoning, bad odors, and detract from aesthetics, breed in bathrooms, kitchens, refrigerators, carpets, etc., and living spaces tend to be more airtight. At present, sterilizing fungi such as mold and bacteria or suppressing their growth has become an important issue in terms of hygiene in the residential environment.

Various disinfectants and fungicides have been proposed to overcome this problem, but due to the relationship between the duration of antibacterial activity and the frequency of treatment, no results have been achieved, especially in the home. is the reality.

The inventors of the present invention have focused on the fact that it is best to perform bacterial sterilization and fungicidal treatments as frequently as possible, and have conducted extensive research to develop a detergent that can achieve this purpose at the same time as daily cleaning. As a result, the use of a combination of anionic or nonionic surfactants, 2-(4-thiazolyl)penzimidazole, and 2,4,4'-trichloro-2'-hydroxydiphenyl ether resulted in synergistic bactericidal and antibacterial effects. It was found that it has a fungal effect, and based on this knowledge, the present invention was made.

That is, the present invention provides anionic or nonionic surfactants, 2-(4-thiazolyl)penzimidazole and 2
, 4,4'-trichloro-2'-hydroxydiphenyl ether.

In the present invention, a water-alcohol mixture is used to dissolve the main ingredient, and examples of this alcohol include lower monohydric alcohols such as methanol, ethanol, propanol, and sopropanol, ethylene glycol, propylene glycol, and glycerin. , lower polyhydric alcohols such as low polymers of polyethylene glycol, or mixtures thereof, and ethanol is preferred.

This water-alcohol mixture usually contains 10 parts by weight of water,
It is used as an alcohol aqueous solution containing 25 to 10 parts by weight of alcohol. Examples of the anionic surfactant used in the present invention include sulfonic acid-based surfactants such as alkylbenzene sulfonates, Q-olefin sulfonates, and paraffin sulfonates, alkyl sulfates,
Examples include sulfate ester type surfactants such as alkyl ethoxy sulfates and alkylphenylethoxy sulfates.On the other hand, nonionic surfactants include polyoxyethylene alkyl ethers and polyoxyethylene surfactants. Examples include alkyl phenyl ethers and sorbitan fatty acid esters.

In the present invention, anionic surfactants or nonionic surfactants may be used alone or in combination of two or more, and the amount of the anionic surfactant or nonionic surfactant in the detergent is 0.1 to 2.
% by weight, preferably in the range of 0.5 to 5% by weight.
In the present invention, 2-(4-thiazolyl)penzimidazole and 2,4,4'tritric. It is necessary to use rho-2'-hydroxydiphenyl ether in combination, with a weight mixing ratio of 9:1 to 1:9, optimally 4:1 to 1:
The range is 4. When used in combination in this way, the antibacterial spectrum is expanded and a synergistic effect is exerted that maintains antibacterial action for a long time at low concentrations against a wide variety of molds and bacteria. The amount of this mixture is 0.1 based on the total amount of detergent.
~2% by weight is suitable. If the amount is less than 0.1% by weight, sufficient antibacterial lasting action cannot be obtained, and if it exceeds 2% by weight, it becomes difficult to prepare a homogeneous detergent. The antibacterial detergent of the present invention thus prepared can be used as is, or it can be sealed in a can together with a propellant and used as a spray.

The antibacterial detergent of the present invention can be used in the same manner as ordinary detergents, and exhibits excellent detergency, bactericidal, and antifungal power.

Therefore, according to the present invention, it is possible to perform sterilization and antifungal treatment on a wide variety of fungi with a long-lasting effect at the same time as cleaning with a simple operation without performing difficult or special operations. Next, the present invention will be explained in more detail with reference to Reference Examples and Examples.

Reference Example 1 2-4-thiazolyl)penzimidazole (TBZ) or 2,4,4'tric.

Rho 2'-hydroxydiphenyl ether (CHOP)
The minimum inhibitory concentration for bacteria or fungi was investigated when each was used alone, when it was used in combination as a mixture of equal amounts, or when this mixture was used in combination with long mirror alkylbenzene sulfonate (LAS). That is, first, the following bacterial and fungal media are prepared. (
Trypto-soy agar medium (for bacteria) [Medium 1000
Ingredients in the ground] Ta Tryptone 15 Soybeptone 5 Sodium chloride
5th evening agar 15th evening {0} Potato dextrose agar toji (for fungi) 0
[Ingredients in medium loo0] Potato infusion solution
200 tabdu sugar
20 evening agar 15 evening Next, a 5-bacterial saline suspension of various bacterial strains (a bacterial suspension for bacteria, a spore suspension for mold) was applied to each of the above medium using a platinum loop. However, in the case of bacteria, 30oo
In the case of mold, the bacteria were cultured for 4 hours at 25oC for 12 hours, and the presence or absence of bacterial growth at various concentrations of the antibacterial agent was examined.

0 The results are shown in Table 1.

Incidentally, as CHOP, Irgasan DP300 (trade name, manufactured by Ciba-Geigi Co., Ltd.) was used. Table 1 *I T/○; Equivalent mixture of TBZ/OHDP *2 L
AS-1; LAS O. T/0 added to 1 weight purple solution *3 LAS-2; LAS T/0 added to 2 weight purple solution
0 added ■ Stahylococcus aureus (
Staphylo-coccus aureus)IA
MIOII■ Escherichia coli (Escherichia coli)
hia coli) IAM1239■ Proteus. vulgaris (Proteus vulga-ris) 1A
M 1203■ Bacillus subtilis (Baeill
us subtilis) INM 1213 ■ Aspergillns nig
er) ATO0 6275 ■ Penicillium cit.rinm 1 rainbow M 7316 ■ Trichoderma viride (Tricho
derma viride) IAM 5141■ Fusarium moniliforme (Fusanummonilif)
orme) IF06349 As is clear from this Table 1, 2-(4-thiazolyl)penzimidazole (TBZ
) and 2,4,4-trichloro-2'-hydroxydiphenyl ether (CHDP) show a remarkable expansion of the antibacterial spectrum and a synergistic effect of antibacterial action.

Reference Example 2 A pile mold resistance test was conducted in accordance with JIS-Z-2911.

That is, ethanol solutions of various concentrations are prepared using TBZ or CHDP alone or a mixture of equal amounts (T/C) of these, and then this solution is mixed with Toyo Paper (No. 2 diameter 3 After soaking the culture medium, it was left indoors for 2 hours, and then it was placed in the center of the culture surface of a designated culture medium in a petri dish, and the test mold spore suspension LCC was evenly sprinkled on top of it. After that, put a lid on the beetle dish and
800 for 14 days, and the state of mold growth was observed and evaluated. The composition of the culture medium used at this time was 100% water and 100% water.
On desk, glucose 409, beptone 10, and agar 25. In addition, the following criteria were used to evaluate the state of mold growth. −; No mold growth observed.

10: Mold growth is observed in 1/3 or less of the area.

Mold growth was observed on an area exceeding 10:1'3.

Furthermore, the mold species in the test mold spore suspension are as follows.

{11 Asbergillus niger (, Aspergi
llusniger) ATCC9642{21 Benicillium luteum (Penicmi mountain mlute dish m
) ATCC 9644 (3 guns Trichoderma T-1 (
Trichoderma T-Table 2 As is clear from this Table 2, 2-(4-thiazolyl)penzimidazole (TBZ) and 2,4,4-trichloro-2-hydro.

(Xydiphenyl ether)CHDP) (T/C
), it has a synergistic effect and exhibits antifungal power for a long time at low concentrations. , *Example 1 Detergents of the present invention were prepared with various blending ratios, and an antifungal property test was conducted in the same manner as in Reference Example 2.

However, the test period was 7 days. The results are shown in Table 3. Note that Table 3 also shows the results of tests conducted for comparison. Table 3 As is clear from Table 3, the detergent of the present invention has excellent anti-fungal properties, and no mold growth was observed even after one week had passed.

Example 2 Freon 12 (Freon 12) was added to each of the four detergent solutions used in Example 1.
An aerosol was prepared by adding a propellant (propellant) at a ratio of 25 m/100 m, and this was sprayed on areas in the home where mold was likely to grow, and the mold growth status was investigated over a period of 6 months. .

The results are shown in Table 4. In this table, 10 indicates that mold is observed, and - indicates that no mold is observed.
Table 4 *○: No treatment From Table 4, it can be seen that the detergent of the present invention can prevent mold growth for a long period of time.

Example 3 To Composition 9.9' of Example 1-A, 0.1 of a bacterial suspension in sterile physiological saline was added, and the change over time in the number of viable bacteria was determined to examine the bactericidal power.

At this time, the number of viable bacteria was measured by the plate pour method using a trypto-soy agar medium, and the determination was made after culturing at 3000 for 48 hours. The results are shown in Table 5.

Table 5■ Shootmonas aeruginosa (Pseudo-
monaS aeruglnosa) IF○ 3447
@ Staphylokochus awareus (Staphylo)
1C0CCus aureus)lAM roll■Escherichia coli
lAM IO97 As can be seen from Table 5, the detergent of the present invention has an excellent bactericidal action that kills bacteria in a short period of time.

Example 4 The wall inside the refrigerator was divided into 8 cm long and 8 horizontal sections, and each section was treated with [1-no treatment, [2] water wiping treatment, {3' wiping treatment with kitchen cleaning liquid at a normal usage concentration, After spraying the detergent of Example 2-A and wiping with gauze, each section was covered with sterile gauze (8 x 8 arcs, 6 layers) that had been soaked in 10 times of sterile physiological saline and preserved. Collect bacteria by wiping, then soak the gauze again in sterile physiological saline to suspend it, use this suspension as a test solution to measure the number of viable bacteria by the plate tear method, and record the measured value. As the number of bacteria remaining in each section, the remaining bacteria rate was calculated for the untreated portion (average value of 10 measurements, 2.0 × 1 pi (cells/c certainty): 100).

The results are shown in Table 6.

Table 6 As can be seen from this table, the number of remaining bacteria in the compartments treated with the detergent of the present invention was extremely small, and the bacteria were almost completely sterilized and cleaned.

Claims (1)

[Claims] 1 Anionic or nonionic surfactant, 2-(4-
An antibacterial detergent characterized by containing thiazolyl)benzimidazole and 2,4,4'-trichloro-2'-hydroxydiphenyl ether.