JPS6142219B2 - - Google Patents
Info
- Publication number
- JPS6142219B2 JPS6142219B2 JP11656376A JP11656376A JPS6142219B2 JP S6142219 B2 JPS6142219 B2 JP S6142219B2 JP 11656376 A JP11656376 A JP 11656376A JP 11656376 A JP11656376 A JP 11656376A JP S6142219 B2 JPS6142219 B2 JP S6142219B2
- Authority
- JP
- Japan
- Prior art keywords
- absorption
- light
- detector
- sample
- wavelength corresponding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000010521 absorption reaction Methods 0.000 claims description 28
- 238000005259 measurement Methods 0.000 claims description 8
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 claims 1
- 238000004868 gas analysis Methods 0.000 claims 1
- 230000031700 light absorption Effects 0.000 claims 1
- 238000004611 spectroscopical analysis Methods 0.000 claims 1
- 230000002123 temporal effect Effects 0.000 claims 1
- 230000007102 metabolic function Effects 0.000 description 10
- 238000000034 method Methods 0.000 description 9
- 150000001875 compounds Chemical class 0.000 description 8
- 238000001727 in vivo Methods 0.000 description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 230000004060 metabolic process Effects 0.000 description 4
- 238000000862 absorption spectrum Methods 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 238000004949 mass spectrometry Methods 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 230000002285 radioactive effect Effects 0.000 description 2
- 206010016654 Fibrosis Diseases 0.000 description 1
- 101700004678 SLIT3 Proteins 0.000 description 1
- 102100027339 Slit homolog 3 protein Human genes 0.000 description 1
- 230000007882 cirrhosis Effects 0.000 description 1
- 208000019425 cirrhosis of liver Diseases 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 230000005976 liver dysfunction Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
Landscapes
- Investigating Or Analysing Materials By Optical Means (AREA)
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Description
【発明の詳細な説明】
本発明は吸光分析により高精度で生体代謝機能
を測定する方法に関するもので、特に13Cをラベ
ルした化合物を投与して生体内代謝により呼気中
に生ずる13CO2と12CO2の比を測定する生体代謝
機能の測定方法において、自然界存在比の13CO2
と12CO2の赤外吸収量が等しくなる波数にて両者
の吸収強度を測定し、その比を記録することを特
徴とする生体代謝機能の測定方法に関するもので
ある。[Detailed Description of the Invention] The present invention relates to a method for measuring biological metabolic functions with high precision using absorption analysis. In particular, by administering a 13 C-labeled compound, the 13 CO 2 and 13 CO 2 generated in exhaled air due to in vivo metabolism are In the measurement method of biological metabolic function that measures the ratio of 12 CO 2 , the natural abundance ratio of 13 CO 2
This invention relates to a method for measuring biological metabolic function, which is characterized by measuring the absorption intensity of both CO 2 and 12 CO 2 at a wave number at which their infrared absorption amounts are equal, and recording the ratio.
従来、放射性同位体である14Cをラベルした化
合物を用いてその化合物の生体内代謝をシンチレ
ーシヨンカウンターにより測定していたが、放射
性同位体の取り扱い、さらに生体への影響などに
より、他の測定法が望まれていた。このような情
勢の中、最近安定同位体の利用が注目され、13C
をラベルした化合物を投与して生体内代謝により
呼気中に生ずる13CO2と12CO2の比を質量分析に
より求め、これによつて代謝機能を測定すること
が行なわれるようになつたが、真空系を用いるこ
とによる装置の取り扱い、保守の困難性、あるい
は高価格という欠点により安易に使用できない問
題があつた。 Traditionally, compounds labeled with the radioactive isotope 14 C were used to measure the in vivo metabolism of the compound using a scintillation counter, but due to the handling of radioactive isotopes and their effects on living organisms, other methods of measurement have become necessary. Law was desired. Under these circumstances, the use of stable isotopes has recently attracted attention, and 13 C
The ratio of 13 CO 2 and 12 CO 2 produced in exhaled breath due to in vivo metabolism is determined by mass spectrometry by administering a labeled compound, and metabolic function is now measured using this method. There are problems in that it cannot be easily used due to the difficulty in handling and maintenance of the device due to the use of a vacuum system, and the high cost.
従つて本発明の目的はこのような問題を解消す
ることである。 It is therefore an object of the present invention to eliminate such problems.
即ち、本発明は吸光分析により生体内の代謝機
能を測定しようとするものであり、13Cをラベル
した化合物を投与して生体内代謝により呼気中に
生ずる13CO2と12CO2の比を測定する生体内代謝
機能の測定方法において、自然界存在比の13CO2
と12CO2の赤外吸収量が等しくなる波数にて両者
の吸収強度を測定し、その比を記録することを特
徴とする生体内代謝機能の測定方法を提供するも
のである。 That is, the present invention aims to measure the metabolic function in the living body by absorption analysis, and the ratio of 13 CO 2 and 12 CO 2 produced in exhaled breath by in vivo metabolism is determined by administering a 13 C-labeled compound. In the method of measuring in vivo metabolic function, the natural abundance ratio of 13 CO 2
The present invention provides a method for measuring metabolic function in a living body, which is characterized by measuring the absorption intensity of both CO 2 and 12 CO 2 at a wave number at which their infrared absorption amounts are equal, and recording the ratio.
本発明において測定波数を限定した理由は高精
度測定を可能とするためである。即ち、二酸化炭
素は赤外領域に吸収を持ち、第１図に示す如く
12CO2と13CO2では中心波数で約60cm-1の吸収帯
シフトが生ずるが、その測定波数を例えば12CO2
について2340cm-1、13CO2について2270cm-1とい
うように選択すると、13CO2と12CO2が自然界比
（13CO2／12CO2＝1.108％）で存在した場合第１
図の吸収スペクトルに示すように著しく13CO2が
小さくなつてしまい12CO2と13CO2の吸収強度比
をそのまゝ測定することはできないからである。
従つて、本発明においてはこのような問題を避け
るために13CO2と12CO2の吸収強度が等しくなる
ような波数、例えば13CO2については2270cm-1付
近、12CO2については2390cm-1及び668cm-1（図示
せず）付近の波数にその測定波数が限定される。 The reason why the measurement wave number is limited in the present invention is to enable high precision measurement. That is, carbon dioxide has absorption in the infrared region, as shown in Figure 1.
12 CO 2 and 13 CO 2 cause an absorption band shift of approximately 60 cm -1 at the center wavenumber, but if the measured wavenumber is changed to 12 CO 2 , for example,
If we choose 2340 cm -1 for 13 CO 2 and 2270 cm -1 for 13 CO 2 , if 13 CO 2 and 12 CO 2 exist at the natural ratio ( 13 CO 2 / 12 CO 2 = 1.108%), then the first
This is because, as shown in the absorption spectrum in the figure, 13 CO 2 becomes significantly smaller, making it impossible to directly measure the absorption intensity ratio of 12 CO 2 and 13 CO 2 .
Therefore, in the present invention, in order to avoid such problems, the wavenumber is set such that the absorption intensities of 13 CO 2 and 12 CO 2 are equal, for example, around 2270 cm -1 for 13 CO 2 and 2390 cm - for 12 CO 2 . The measurement wavenumber is limited to wavenumbers around 1 and 668cm -1 (not shown).
従つて、本発明の場合、13CO2をラベルした化
合物が投与されていない限り、13CO2と12CO2の
吸収強度比は１であり、投与された化合物が代謝
されるにつれ増加する吸収強度比が正確にとらえ
られる。 Therefore, in the case of the present invention, unless a 13 CO 2 labeled compound is administered, the absorption intensity ratio of 13 CO 2 to 12 CO 2 is 1, and the absorption intensity increases as the administered compound is metabolized. The intensity ratio can be accurately captured.
第２図は本発明の方法を実施するための装置の
光学系の一例を示すものであり、13Cをラベルし
た化合物を生体内で代謝させ、代謝された呼気が
吸収セル１内に導かれる。光源２からの光はこの
吸収セル１内を通過することにより12CO2及び
13CO2の吸収を受け、入口スリツト３を経て凹面
回折格子４に入射し、出口スリツト５，５′に集
光される。この波数走査は凹面回折格子によらず
平面回折格子を使用して行なうこともできる。出
口スリツト位置は前述のように例えば13CO2につ
いて2270cm-1付近、12CO2については2390cm-1付
近に設定するのが望ましい。出口スリツト５，
５′を通過した両光束はチヨツパー６により夫々
凹面鏡７，７′を経て交互に検知器８に導かれ
る。 FIG. 2 shows an example of an optical system of an apparatus for carrying out the method of the present invention, in which a 13 C-labeled compound is metabolized in a living body, and the metabolized exhaled air is guided into the absorption cell 1. . The light from the light source 2 passes through the absorption cell 1, resulting in 12 CO 2 and
13 CO 2 is absorbed, enters the concave diffraction grating 4 through the entrance slit 3, and is focused on the exit slits 5, 5'. This wave number scanning can also be performed using a plane diffraction grating instead of using a concave diffraction grating. As mentioned above, it is desirable to set the exit slit position, for example, around 2270 cm -1 for 13 CO 2 and around 2390 cm -1 for 12 CO 2 . Exit slit 5,
Both light fluxes passing through the detector 5' are alternately guided by a chopper 6 to a detector 8 via concave mirrors 7 and 7', respectively.
検知器８からは13CO2と12CO2の吸収強度に
夫々対応した出力が生じ、これらの信号が、第３
図に示す如く前置増巾器９、主増巾器１０を介し
てサンプルホールド回路１１に導かれる。このサ
ンプルホールド回路１１にはチヨツパー６の回転
と同期させこれらの信号を処理するための同期信
号が導かれている。この同期信号は同期号用ラン
プ１２と同期信号用検知器１３で検出し、その出
力を同期信号処理回路１４で処理することにより
得られる。この同期信号によりサンプルホールド
回路１１内で処理された12CO2の吸収強度に対応
する信号は誤差増巾器１５を介し主増巾器１０に
送られてその利得を調整するようになつており、
従つて12CO2の吸収強度に対応する入力信号が常
に一定値となる。従つて、サンプルホールド回路
１１からの13CO2の吸収強度に対応する信号は、
それ自体12CO2の吸収強度に対応する信号との比
を表わすことになり、フイルター１６を介し記録
計１７に導びかれこれを表示することになる。 Outputs corresponding to the absorption intensities of 13 CO 2 and 12 CO 2 are generated from the detector 8, and these signals are
As shown in the figure, the signal is guided to a sample and hold circuit 11 via a preamplifier 9 and a main amplifier 10. A synchronizing signal for processing these signals in synchronization with the rotation of the chopper 6 is led to the sample and hold circuit 11. This synchronization signal is detected by a synchronization signal lamp 12 and a synchronization signal detector 13, and its output is processed by a synchronization signal processing circuit 14. A signal corresponding to the absorption intensity of 12 CO 2 processed in the sample and hold circuit 11 by this synchronization signal is sent to the main amplifier 10 via the error amplifier 15 to adjust its gain. ,
Therefore, the input signal corresponding to the absorption intensity of 12 CO 2 is always a constant value. Therefore, the signal corresponding to the absorption intensity of 13 CO 2 from the sample and hold circuit 11 is:
The signal itself represents the ratio with the signal corresponding to the absorption intensity of 12 CO 2 , and is led to the recorder 17 via the filter 16 and displayed.
このように測定した測定結果の一例は第４図に
示されている。第４図はグルコースに13Cをラベ
ルして人体に投与した場合を示すもので、正常人
の場合呼気中に投与後直ちに13CO2の増加が認め
られ、肝臓によりグルコースが極めて効果的に代
謝されることが示されている（曲線）が、肝臓
機能障害のある人（曲線、この場合肝硬変）で
は曲線の立ち上りが鈍く、その減少もダラダラし
ており、従つて代謝機能が正常に営まれていない
ことがわかる。 An example of the measurement results measured in this manner is shown in FIG. Figure 4 shows the case in which glucose is labeled with 13 C and administered to the human body. In a normal person, an increase in 13 CO 2 is observed immediately after administration in exhaled breath, indicating that glucose is extremely effectively metabolized by the liver. However, in people with liver dysfunction (curve, in this case cirrhosis), the rise of the curve is slow and the decline is slow, indicating that metabolic function is not operating normally. It turns out that it is not.
以上の説明で明らかなように、本発明の方法
は、自然界存在比の13CO2と12CO2の赤外吸収量
が等しくなる波数にて両者の吸収強度を測定し、
これを記録するようにしているので、質量分析に
よるものよりも遥かに容易に生体代謝機能を測定
することができるものである。 As is clear from the above explanation, the method of the present invention measures the absorption intensity of 13 CO 2 and 12 CO 2 at a wave number where the infrared absorption amounts of both 13 CO 2 and 12 CO 2 are equal in abundance in nature,
Since this is recorded, biological metabolic functions can be measured much more easily than by mass spectrometry.
第１図は自然界存在比で13CO2と12CO2が存在
している場合の二酸化炭素の吸収スペクトルを示
す図であり、第２図及び第３図は夫々本発明方法
を実施するための装置の光学系と電気系を示す図
であり、第４図は代謝機能の測定結果の一例を示
す図である。
１……吸収セル、２……光源、３……入口スリ
ツト、４……凹面回折格子、５，５′……出口ス
リツト、６……チヨツパー、７，７′……凹面
鏡、８……検知器、９……前置増巾器、１０……
主増巾器、１１……サンプルホールド回路、１２
……同期信号用ランプ、１３……同期信号用検知
器、１４……同期信号処理回路、１５……誤差増
巾器、１６……フイルター、１７……記録計。
Figure 1 is a diagram showing the absorption spectrum of carbon dioxide when 13 CO 2 and 12 CO 2 are present in the natural abundance ratio, and Figures 2 and 3 are diagrams showing the absorption spectrum for carrying out the method of the present invention, respectively. FIG. 4 is a diagram showing an optical system and an electrical system of the apparatus, and FIG. 4 is a diagram showing an example of measurement results of metabolic function. 1... Absorption cell, 2... Light source, 3... Entrance slit, 4... Concave diffraction grating, 5, 5'... Exit slit, 6... Chopper, 7, 7'... Concave mirror, 8... Detection Container, 9... Preamplifier, 10...
Main amplifier, 11...Sample and hold circuit, 12
... Synchronization signal lamp, 13 ... Synchronization signal detector, 14 ... Synchronization signal processing circuit, 15 ... Error amplifier, 16 ... Filter, 17 ... Recorder.
Claims (1)
の吸収を測定する試料セルと、該試料セルを透過
した光源からの光を分光する手段と、分光された
光の強度を検出する検出器とを備えたガス分析装
置において、 13CO2と12CO2の赤外波長領域での吸収が等し
くなるような異なる波長に、試料セルを透過した
光を分光手段により分光し、分光した光を検出器
にて検出し、12CO2と13CO2の吸収の比を測定記
録することを特徴とする重炭酸ガス分析測定装
置。 ２ 分光手段により分光された12CO2の吸収に対
応する波長の光と、13CO2の吸収に対応する波長
の光とをチヨツパーにより交互に断続させて検出
器に導入し、12CO2の吸収に対応する波長の光に
よる検出器からの出力を一定値に制御することに
より、13CO2の吸収の時間的変化を測定すること
を特徴とする特許請求の範囲第１項記載の装置。[Scope of Claims] 1. A sample cell into which a measurement sample is introduced and the absorption of light from a light source of the sample is measured; a means for dispersing the light from the light source transmitted through the sample cell; and a means for dispersing the light from the light source that has passed through the sample cell; In a gas analyzer equipped with a detector that detects the intensity of A bicarbonate gas analysis and measurement device characterized by performing spectroscopy, detecting the spectroscopic light with a detector, and measuring and recording the ratio of absorption of 12 CO 2 and 13 CO 2 . 2 Light with a wavelength corresponding to the absorption of 12 CO 2 separated by the spectroscopic means and light with a wavelength corresponding to the absorption of 13 CO 2 are alternately interrupted by a chopper and introduced into the detector, and the light with a wavelength corresponding to the absorption of 12 CO 2 is introduced into the detector. 2. The apparatus according to claim 1, characterized in that the temporal change in the absorption of 13 CO 2 is measured by controlling the output from the detector using light of a wavelength corresponding to the absorption to a constant value.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11656376A JPS5342889A (en) | 1976-09-30 | 1976-09-30 | Measuring method of methabolism function of organ |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11656376A JPS5342889A (en) | 1976-09-30 | 1976-09-30 | Measuring method of methabolism function of organ |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5342889A JPS5342889A (en) | 1978-04-18 |
| JPS6142219B2 true JPS6142219B2 (en) | 1986-09-19 |
Family
ID=14690195
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP11656376A Granted JPS5342889A (en) | 1976-09-30 | 1976-09-30 | Measuring method of methabolism function of organ |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5342889A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01132323U (en) * | 1988-03-04 | 1989-09-08 |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07117544B2 (en) * | 1991-06-28 | 1995-12-18 | 株式会社生体科学研究所 | Method and device for analyzing kinetics of drug or substrate |
| JPH05296922A (en) * | 1992-04-16 | 1993-11-12 | Japan Radio Co Ltd | Carbon isotope analyzing instrument |
| JP2775558B2 (en) * | 1992-10-16 | 1998-07-16 | 日本無線株式会社 | Isotope ratio correction method and isotope ratio correction device |
| JPH0769328B2 (en) * | 1992-10-30 | 1995-07-26 | 株式会社生体科学研究所 | Metabolism analysis method |
| JP2686698B2 (en) * | 1992-11-05 | 1997-12-08 | 日本無線株式会社 | Isotope ratio analysis method and device |
| FI107194B (en) * | 1996-03-14 | 2001-06-15 | Instrumentarium Oy | Analysis of gas mixtures by infrared method |
| WO2018173348A1 (en) | 2017-03-22 | 2018-09-27 | 株式会社Ihi | Low-temperature tank and method for manufacturing same |
-
1976
- 1976-09-30 JP JP11656376A patent/JPS5342889A/en active Granted
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01132323U (en) * | 1988-03-04 | 1989-09-08 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5342889A (en) | 1978-04-18 |
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