JPS6217700B2 - - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a migration diffusion device used for protein analysis by immunoelectrophoresis using agar gel in the field of clinical testing. The purpose of the present invention is to provide a migration diffusion device that can perform the following.

Conventionally, in immunoelectrophoresis, etc., an agarose gel-like material is used as a support for electrophoresis, and this support is placed between two electrically insulated tanks containing a buffer solution using a sponge or the like. Generally employed is a method in which the support is arranged in a bridging manner and electrophoresis is performed by applying electricity to the support. Alternatively, instead of a sponge, one end of a piece of filter paper or agarose is brought into contact with the support.
A method of electrically connecting the other end by dipping it in a buffer solution is also used. The support is usually prepared by dissolving agarose powder, pouring it onto a glass plate or plastic film, and solidifying it into a gel-like state. This support must be provided with a sample injection hole and an antiserum injection groove, but the sample injection hole and antiserum injection groove are prepared by the analyst or the like before the operation. Therefore, the conventional immunoelectrophoresis method described above has various drawbacks as described below.

(1) Skill is required to prepare the support, holes for sample injection, and grooves for antiserum injection, and it is difficult to always prepare a uniform support.

(2) Migration unevenness tends to occur due to poor contact when electricity is applied to the support.

(3) It is difficult to adopt a cooling method to prevent heat generation and drying during electrophoresis.

(4) The support easily peels off from the glass plate or plastic film and penetrates into the gap through the sample injection hole or antiserum injection groove, which may prevent accurate electrophoresis and immunodiffusion.

The present invention was made in view of the above points, and consists of a migration diffusion tank filled with a gel-like support such as agar or agarose, and a connecting tank filled with a gel-like support extending downward at both ends of the migration diffusion tank. , a buffer solution tank equipped with an electrode provided on the outside of this connection tank, a specimen injection hole and an antiserum injection groove are provided in the flat part of the support, and a buffer solution tank is provided between the buffer solution tank and the connection tank. By providing a communication hole for electrically connecting the two tanks and further providing a ventilation hole at the top of the buffer tank to construct a migration diffusion device, the above-mentioned drawbacks were solved, i.e., uniformity was maintained at all times. It is possible to create a support, prevent uneven migration due to poor contact during energization, and prevent drying of the support, which allows accurate migration and diffusion. The purpose is to provide a migration diffusion device.

Hereinafter, the configuration of the present invention will be explained based on embodiments shown in the drawings. Reference numeral 1 denotes a flat box-shaped electrophoretic diffusion tank with an open top, and leg-shaped connecting tanks 2 are connected to the bottom of both ends of the electrophoretic diffusion tank 1 so that the insides thereof communicate with each other. The electrophoretic diffusion tank 1 and the connection tank 2 are filled with a gel-like support 3 for electrophoretic diffusion made of agar gel such as agar or agarose. The electrophoretic diffusion tank 1 is preferably made of transparent synthetic resin for optical measurement after operation. The thickness of the support in the migration diffusion tank 1 varies depending on the object to be measured, but is approximately several hundred microns to several millimeters.
A uniform thickness is formed between these values. A plurality of specimen injection holes 4 and antiserum injection grooves 5 are provided in the support in the electrophoretic diffusion tank 1 . One or more antiserum injection grooves 5 are provided in the current direction, that is, from one connecting tank to the other connecting tank. Furthermore, a buffer solution tank 7 equipped with an electrode 6 is connected to the outside of the connection tank 2, and a communication hole 8 is provided between the buffer solution tank 7 and the connection tank 2 to electrically connect the two tanks. It is being Further, a vent hole 9 is provided in the upper part of the buffer solution tank 7 to vent gas during electrophoresis. 10 is a buffer solution filled in a buffer solution tank. Note that the surface of the support 3 and the ventilation holes 9 are
It is preferable to cover it with a thin plastic film before use, and remove it immediately before use to prevent moisture from evaporating.

To perform immunoelectrophoresis using the electrophoretic diffusion device of the present invention configured as described above, first, a sample is injected into the sample injection hole 4, and when a voltage is applied from the outside through the electrode 6, the buffer solution tank 7 is connected. A voltage is applied to the support 3 through the hole 8 to perform migration. After the electrophoresis is completed, when antiserum is injected into the antiserum injection groove 5, the antibody and antigen react due to immunodiffusion, and a sedimentation line is observed. This can be traced using an optical device, or stained and photographed for analysis. The type and content of protein are determined. The electrophoretic diffusion device of the present invention can be used alone, or can be installed in an automatic analyzer to perform automatic dispensing and automatic optical measurement and analysis.
Furthermore, even during long-term storage, since the buffer solution is constantly replenished into the support, it is possible to prevent the inclusion of air bubbles due to drying of the support and the formation of gaps between the support and the wall of the electrophoretic diffusion tank. Furthermore, since we can always supply products of constant quality, we can always obtain stable and highly accurate analysis results. Note that when the electrophoretic diffusion tank is formed of a transparent material, optical measurements and photography can be easily performed.

Since the present invention is constructed as described above, the device can be used as a standalone device or by being set in a cassette type in an automatic analyzer, and highly accurate analysis can be performed without requiring any skill. Furthermore, no pretreatment is required, and measurements can always be performed under the same conditions. Furthermore, it has the effect of being stable for long-term storage.

[Brief explanation of the drawing]

FIG. 1 is an explanatory longitudinal cross-sectional view showing one embodiment of the electrophoretic diffusion device of the present invention, and FIG. 2 is a partially enlarged cross-sectional view of the support. DESCRIPTION OF SYMBOLS 1... Electrophoresis diffusion tank, 2... Connection tank, 3... Support, 4... Sample injection hole, 5... Antiserum injection groove, 6... Electrode, 7... Buffer solution tank, 8... ...Communication hole, 9...Vent hole, 10...Buffer solution.

Claims (1)

[Claims] 1 Connecting leg-shaped tanks are connected to the bottom of both ends of a flat box-shaped migration diffusion tank with an open top so that the insides communicate with each other, and agar, agarose, etc. are placed inside the migration diffusion tank and the connection tank. A gel-like support for electrophoresis diffusion is filled, a sample injection hole and an antiserum injection groove are provided in the support in the electrophoresis diffusion tank, and a buffer tank equipped with an electrode is provided outside the connection tank. A communication hole is provided between the buffer solution tank and the connection tank to electrically connect the two tanks, and a ventilation hole is provided in the upper part of the buffer solution tank. Diffusion device.