JPS6229016B2 - - Google Patents
Info
- Publication number
- JPS6229016B2 JPS6229016B2 JP5025380A JP5025380A JPS6229016B2 JP S6229016 B2 JPS6229016 B2 JP S6229016B2 JP 5025380 A JP5025380 A JP 5025380A JP 5025380 A JP5025380 A JP 5025380A JP S6229016 B2 JPS6229016 B2 JP S6229016B2
- Authority
- JP
- Japan
- Prior art keywords
- wavelengths
- sample
- absorption
- absorbance
- difference
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000010521 absorption reaction Methods 0.000 claims description 30
- 230000005856 abnormality Effects 0.000 claims description 16
- 238000002835 absorbance Methods 0.000 claims description 16
- 239000007788 liquid Substances 0.000 claims description 13
- 238000005259 measurement Methods 0.000 claims description 13
- 230000031700 light absorption Effects 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 10
- 238000006243 chemical reaction Methods 0.000 claims description 9
- 238000004040 coloring Methods 0.000 claims description 9
- 210000002966 serum Anatomy 0.000 claims description 7
- 230000002159 abnormal effect Effects 0.000 claims description 6
- 206010018910 Haemolysis Diseases 0.000 claims description 3
- 206010023126 Jaundice Diseases 0.000 claims description 3
- 210000001268 chyle Anatomy 0.000 claims description 3
- 230000008588 hemolysis Effects 0.000 claims description 3
- 210000002381 plasma Anatomy 0.000 claims description 2
- 238000004611 spectroscopical analysis Methods 0.000 claims description 2
- 238000011088 calibration curve Methods 0.000 description 9
- 230000035945 sensitivity Effects 0.000 description 6
- 238000004445 quantitative analysis Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000012742 biochemical analysis Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000000691 measurement method Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/314—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths
Landscapes
- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Description
【発明の詳細な説明】
本発明は比色計を使用する生化学自動分析にお
ける濃度変換方法に関するものである。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a concentration conversion method in automatic biochemical analysis using a colorimeter.
従来被検物質の目的成分を正確に定量分析する
ためには血清盲検をとるか又は除蛋白を行う等の
方法があつたが、その後簡単な２波長測定法が開
発された。しかし２波長のみを用いる測定法では
試料の吸収異常の要因である乳び、黄疸、溶血等
のため試料の目的成分以外の吸収、例えば血清の
色、濁り等の影響を除去する効果が不充分であ
り、しかも感度不足等の問題が生じる。従つてこ
れを解決するために特開昭54−84781号公報に記
載されているような多波長による吸光光度分析法
が開発された。 Conventionally, in order to accurately quantitatively analyze the target component of a test substance, methods such as serum blind testing or protein removal were used, but later a simple two-wavelength measurement method was developed. However, measurement methods that use only two wavelengths are insufficiently effective in removing absorption of components other than the target components of the sample, such as serum color and turbidity, due to chyle, jaundice, hemolysis, etc., which are causes of sample absorption abnormalities. Moreover, problems such as insufficient sensitivity arise. Therefore, in order to solve this problem, a spectrophotometric analysis method using multiple wavelengths was developed as described in Japanese Patent Application Laid-open No. 84781/1983.
しかしこの場合には、上記吸収異常の要因はほ
ぼ除去されるが多波長測定例えば少くとも４波長
測定による演算で多数の測定値を得るようにして
いる関係上、多波長測定のデータが増大すればす
るほど、演算を行うためのＡ−Ｄ変換過程で導入
される分解能の最小単位の誤差が増大する。 However, in this case, although the causes of the above-mentioned absorption abnormalities are almost eliminated, since a large number of measured values are obtained by calculations using multi-wavelength measurements, for example, at least four wavelength measurements, the data of multi-wavelength measurements increases. As the number of pixels increases, the error in the minimum unit of resolution introduced in the A-D conversion process for performing calculations increases.
本発明の目的は、生化学分析において試料が高
脂血清、高ビリルビン血清、溶血血清等の測定妨
害要因を含む場合においても試料の測定正確度を
高めるようにした濃度変換方法を提供せんとする
にある。 An object of the present invention is to provide a concentration conversion method that increases the accuracy of sample measurement in biochemical analysis even when the sample contains measurement-interfering factors such as high-lipid serum, high-bilirubin serum, and hemolyzed serum. It is in.
本発明の他の目的は多波長測定により得られた
データを基とし、これから上記吸収異常の要因に
よる影響をできるだけ少くし得るように適宜選択
した２波長を用いて測定を行い、未知試料の濃度
変換を行う濃度変換方法を提供せんとするにあ
る。 Another object of the present invention is to conduct measurements based on data obtained by multi-wavelength measurements using two wavelengths appropriately selected to minimize the effects of the above-mentioned absorption abnormalities, and to determine the concentration of unknown samples. The object of the present invention is to provide a concentration conversion method for performing conversion.
本発明濃度変換方法は比色計を用いて濃度が未
知の血清、血しよう試料を測定するに当り、先ず
呈色液の光吸収特性および上記試料の吸収異常の
要因（乳び、黄疸、溶血）及びその程度の多波長
で分光分析して測定し、これら多波長測定結果を
基とし、多波長のうちから、吸収異常の上記試料
の場合は、波長λa，λbにおける呈色液の吸光度
の差をＥ1〓a-〓b、吸収異常の上記試料の吸光度
の差をＥ2〓a-〓b及び波長λa，λcにおける呈色
液の吸光度の差をＥ1〓a-〓c、吸収異常の上記試
料の吸光度の差をＥ2〓a-〓cとしたときに
Ｅ２〓ａ−〓ｂ／Ｅ１〓ａ−〓ｂ＜Ｅ２〓ａ−
〓ｃ／Ｅ１〓ａ−〓ｃ
（λa＜λb＜λc）
の関係を満足する場合は、波長λa，λbを選択
し、
Ｅ２〓ａ−〓ｂ／Ｅ１〓ａ−〓ｂ＞Ｅ２〓ａ−
〓ｃ／Ｅ１〓ａ−〓ｃ
（λa＜λb＜λc）
の関係を満足する場合は、波長λa，λcを選択
し、吸収が正常な上記試料の場合には、波長λ
a，λcを選択して使用し、未知の上記試料の濃度
変換を行うようにしたことを特徴とする。 In the concentration conversion method of the present invention, when measuring a serum or blood plasma sample of unknown concentration using a colorimeter, we first check the light absorption characteristics of the coloring liquid and the causes of abnormal absorption of the sample (chyle, jaundice, hemolysis). ) and multiple wavelengths of that degree, and based on these multiple wavelength measurement results, among the multiple wavelengths, in the case of the above sample with absorption abnormality, the coloring liquid at wavelengths λ a and λ b is determined. The difference in absorbance is E 1 〓 a- 〓 b , the difference in absorbance of the above sample with absorption abnormality is E 2 〓 a- 〓 b, and the difference in the absorbance of the colored liquid at wavelengths λ a and λ c is E 1 〓 a- 〓 c , and when the difference in absorbance of the above sample with absorption abnormality is E 2 〓 a- 〓 c , E 2 〓 a- 〓 b /E 1 〓 a- 〓 b <E 2 〓 a-
If the relationship 〓 c /E 1 〓 a- 〓 c (λ a < λ b < λ c ) is satisfied, wavelengths λ a and λ b are selected, and E 2 〓 a- 〓 b /E 1 〓 a - 〓 b > E 2 〓 a-
If the relationship 〓 c /E 1 〓 a - 〓 c (λ a < λ b < λ c ) is satisfied, wavelengths λ a and λ c are selected, and in the case of the above sample with normal absorption, the wavelength is λ
The present invention is characterized in that a and λ c are selected and used to convert the concentration of the unknown sample.
図面につき本発明を説明する。 The invention will be explained with reference to the drawings.
本発明では先ず最初多波長分光を行う光電比色
計を有する生化学自特分析装置により、測定１項
目に対し多波長で数種類の試料及び呈色液の光吸
収特性を夫々個別に測定する。斯る測定結果を第
１図に、呈色液の光吸収特性１、吸収異常のある
試料の光吸収特性２、吸収が正常な試料の光吸収
特性３として夫々示す。 In the present invention, first, the light absorption characteristics of several types of samples and coloring liquids are individually measured at multiple wavelengths for one measurement item using a biochemical characteristic analyzer having a photoelectric colorimeter that performs multi-wavelength spectroscopy. The measurement results are shown in FIG. 1 as light absorption characteristic 1 of the coloring liquid, light absorption characteristic 2 of the sample with abnormal absorption, and light absorption characteristic 3 of the sample with normal absorption.
本発明の１例では、波長λa及びλbの２波長を
使用した場合に対する検査線Ａと、波長λa及び
λcの２波長を使用した場合に対する検量線Ｂと
を夫々作成し、これを第２図に示す。即ち、検量
線Ａは波長λa及びλbの夫々の吸光度の差に対応
すると共に検量線Ｂは波長λa及びλcの夫々の吸
光度の差に対応するものである。 In one example of the present invention, a test line A for the case where two wavelengths λ a and λ b are used and a calibration curve B for the case when two wavelengths λ a and λ c are used are created, respectively. is shown in Figure 2. That is, the calibration curve A corresponds to the difference in absorbance between wavelengths λ a and λ b , and the calibration curve B corresponds to the difference in absorbance between wavelengths λ a and λ c .
吸収異常のある試料を用いて定量分析を行う場
合には例えば第１図に示す例では種々の波長のう
ちから波長としてλa及びλbの２波長を選択して
使用し、この際次式
Ｅ２〓ａ−〓ｂ／Ｅ１〓ａ−〓ｂ＜Ｅ２〓ａ−
〓ｃ／Ｅ１〓ａ−〓ｃ
で示される関係が満足される条件を設定すれば、
波長λa及びλcの２波長を選択したときに比べ、
試料の吸収による影響を少なくすることができ
る。 When performing quantitative analysis using a sample with an absorption abnormality, for example, in the example shown in Figure 1, two wavelengths, λ a and λ b , are selected from various wavelengths and used, and in this case, the following equation is used. E 2 〓 a- 〓 b /E 1 〓 a- 〓 b <E 2 〓 a-
〓 c /E 1 〓 a- 〓 If we set the condition that the relationship shown by c is satisfied,
Compared to selecting two wavelengths, λ a and λ c ,
The influence of sample absorption can be reduced.
又、吸収異常のある試料を用いて定量分析を行
う際、上記関係が逆の場合、即ち
Ｅ２〓ａ−〓ｂ／Ｅ１〓ａ−〓ｂ＞Ｅ２〓ａ−
〓ｃ／Ｅ１〓ａ−〓ｃ
の関係が成立する場合にはλa及びλcの２波長を
選択する。 Moreover, when performing quantitative analysis using a sample with absorption abnormality, if the above relationship is reversed, that is, E 2 〓 a- 〓 b /E 1 〓 a- 〓 b >E 2 〓 a-
If the relationship 〓 c /E 1 〓 a- 〓 c holds true, two wavelengths λ a and λ c are selected.
しかし第２図の検量線から明らかなように波長
λa及びλbの２波長を選択して用いる場合には吸
光度／濃度で表わされる感度は波長λa及びλcの
２波長を選択して用いる場合の感度よりも小さく
なり従つて吸光度の誤差は濃度に大きく影響する
ようになる。これがため、吸収が正常な試料を用
いて定量分析を行う場合にはその吸収が少ないた
め波長としてλa及びλcの２波長を選択して使用
し、吸光度／濃度で表わされる感度を大きくとり
得るようにする。 However, as is clear from the calibration curve in Figure 2, when two wavelengths λ a and λ b are selected and used, the sensitivity expressed as absorbance/concentration is determined by selecting two wavelengths λ a and λ c . The sensitivity will be lower than that in use, and therefore, errors in absorbance will greatly affect the concentration. For this reason, when performing quantitative analysis using a sample with normal absorption, two wavelengths, λ a and λ c , are selected and used because the absorption is low, and the sensitivity expressed as absorbance/concentration is increased. Try to get it.
斯様に本発明では試料の吸収を別途に測定し、
その異常の要因及びその程度を考慮し、斯る吸収
異常の要因による影響が最も少なくなるような２
波長を選択して使用し、これにより未知試料に呈
色液を反応させて選択した２波長による吸光度を
測定し、その差の値から濃度の変換を行うように
する。 In this way, in the present invention, the absorption of the sample is measured separately,
Considering the cause of the abnormality and its degree, select the two methods that will minimize the influence of the abnormality
A wavelength is selected and used, the unknown sample is reacted with a coloring liquid, the absorbance at the two selected wavelengths is measured, and the concentration is converted from the value of the difference.
上述した第１図に示す例では吸収が正常な試料
に対しては使用する２波長の波長間隔を広くする
ことにより充分な感度が得られるようにすると共
に吸収異常の試料に対しては使用する２波長の波
長間隔を狭くすることにより精度を低下させても
正確度を向上せしめるようにすることができる。 In the example shown in Figure 1 mentioned above, sufficient sensitivity is obtained by widening the wavelength interval between the two wavelengths used for samples with normal absorption, and at the same time, sufficient sensitivity is obtained for samples with abnormal absorption. By narrowing the wavelength interval between two wavelengths, accuracy can be improved even if accuracy is reduced.
上述した例では測定する２波長を選択決定した
後、この２波長によつて呈色液と反応させた試料
の吸光度を夫々測定し、その差に対する検量線を
用いて濃度変換を行つたが、本発明はこれに限定
されるものではなく、検量線を用いる代りに、上
記選択２波長によつて測定した未知試料の吸光度
を、予め濃度の変換係数が記憶されているコンピ
ユータに導入することによつても濃度変換を行う
ことができる。 In the above example, after selecting two wavelengths to be measured, the absorbance of the sample reacted with the coloring liquid was measured using these two wavelengths, and the concentration was converted using a calibration curve for the difference. The present invention is not limited to this, but instead of using a calibration curve, the absorbance of an unknown sample measured at the two selected wavelengths is introduced into a computer in which concentration conversion coefficients are stored in advance. Concentration conversion can be performed even if the image is distorted.
上述したように本発明によれば呈色液及び種々
の試料を個別に多波長で測定して試料の吸収異常
の要因及びその程度を測定しこれら多波長による
測定結果から吸収異常の試料及び吸収正常の試料
に対し前記試料の吸収異常の要因による影響が最
も少くなるような２波長を選択し、この選択２波
長により未知の試料の濃度変換を行うようにする
ため、多波長による測定よりも構成が簡単とな
り、操作が簡単となり、しかも作業能率が向上す
る利点を有する。 As described above, according to the present invention, the coloring liquid and various samples are individually measured at multiple wavelengths to determine the cause and degree of the absorption abnormality of the sample, and from the measurement results at these multiple wavelengths, the sample with the absorption abnormality and the absorption abnormality can be determined. In order to select two wavelengths that will minimize the influence of the abnormal absorption factor of the sample on a normal sample, and convert the concentration of an unknown sample using these two selected wavelengths, this method is more effective than measurement using multiple wavelengths. It has the advantages of simple configuration, easy operation, and improved work efficiency.
第１図は呈色液の光吸収特性及び試料の光吸収
特性を示す特性図、第２図は選択した２波長を使
用することにより作成した検量線を示す特性図で
ある。
１……呈色液の光吸収特性、２……吸収異常の
ある試料の光吸収特性、３……吸収正常な試料の
光吸収特性、λa，λb，λc……測定波長、Ａ…
…波長λa及びλbを選択した場合に対する検量
線、Ｂ……波長λa及びλcを選択した場合に対す
る検量線。
FIG. 1 is a characteristic diagram showing the light absorption characteristics of the coloring liquid and the light absorption characteristics of the sample, and FIG. 2 is a characteristic diagram showing a calibration curve created by using two selected wavelengths. 1...Light absorption characteristics of the colored liquid, 2...Light absorption characteristics of a sample with absorption abnormality, 3...Light absorption characteristics of a sample with normal absorption, λ a , λ b , λ c ... Measurement wavelength, A …
...Calibration curve for the case where wavelengths λ a and λ b are selected, B... Calibration curve for the case where wavelengths λ a and λ c are selected.
Claims (1)
試料を測定するに当り、先ず呈色液の光吸収特性
および上記試料の吸収異常の要因（乳び、黄疸、
溶血）及びその程度を多波長で分光分析して測定
し、これら多波長測定結果を基とし、多波長のう
ちから、吸収異常の上記試料の場合は、波長λ
a，λbにおける呈色液の吸光度の差をＥ1〓a-〓
ｂ、吸収異常の上記試料の吸光度の差をＥ2〓a-
〓ｂ及び波長λa，λcにおける呈色液の吸光度の
差をＥ1〓a-〓b、吸収異常の上記試料の吸光度の
差をＥ2〓a-〓cとしたときに Ｅ２〓ａ−〓ｂ／Ｅ１〓ａ−〓ｂ＜Ｅ２〓ａ−
〓ｃ／Ｅ１〓ａ−〓ｃ （λa＜λb＜λc） の関係を満足する場合は、波長λa、λbを選択
し、 Ｅ２〓ａ−〓ｂ／Ｅ１〓ａ−〓ｂ＞Ｅ２〓ａ−
〓ｃ／Ｅ１〓ａ−〓ｃ （λa＜λb＜λc） の関係を満足する場合は、波長λa，λcを選択
し、吸収が正常な上記試料の場合は、波長λa，
λcを選択して使用し、未知の上記試料の濃度変
換を行うようにしたことを特徴とする濃度変換方
法。[Claims] 1. When measuring a serum or blood plasma sample of unknown concentration using a colorimeter, first the light absorption characteristics of the coloring liquid and the causes of abnormal absorption of the sample (chyle, jaundice,
Hemolysis) and its degree are measured by spectroscopic analysis at multiple wavelengths, and based on these multiple wavelength measurement results, from among the multiple wavelengths, in the case of the above sample with abnormal absorption, select the wavelength λ.
The difference in absorbance of the colored liquid at a and λ b is E 1 〓 a- 〓
b , the difference in absorbance of the above sample with absorption abnormality is E 2 〓 a-
〓 b , and the difference in the absorbance of the colored liquid at wavelengths λ a and λ c is E 1 〓 a- 〓 b , and the difference in the absorbance of the above sample with absorption abnormality is E 2 〓 a- 〓 c , then E 2 〓 a- 〓 b /E 1 〓 a- 〓 b <E 2 〓 a-
If the relationship 〓 c /E 1 〓 a- 〓 c (λ a < λ b < λ c ) is satisfied, wavelengths λ a and λ b are selected, and E 2 〓 a- 〓 b /E 1 〓 a - 〓 b > E 2 〓 a-
If the following relationship is satisfied: 〓 c /E 1 〓 a - 〓 c (λ a < λ b < λ c ), wavelengths λ a and λ c are selected, and in the case of the above sample with normal absorption, the wavelength λ is selected. a ,
A concentration conversion method characterized in that λ c is selected and used to convert the concentration of the unknown sample.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5025380A JPS56147043A (en) | 1980-04-18 | 1980-04-18 | Method for conversion of concentration |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5025380A JPS56147043A (en) | 1980-04-18 | 1980-04-18 | Method for conversion of concentration |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS56147043A JPS56147043A (en) | 1981-11-14 |
| JPS6229016B2 true JPS6229016B2 (en) | 1987-06-24 |
Family
ID=12853811
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5025380A Granted JPS56147043A (en) | 1980-04-18 | 1980-04-18 | Method for conversion of concentration |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS56147043A (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61237040A (en) * | 1985-04-15 | 1986-10-22 | Iiosu:Kk | Optimum wavelength selection system for component analyzer |
| JP4881855B2 (en) * | 2005-03-29 | 2012-02-22 | シスメックス株式会社 | Sample analysis method and sample analyzer |
-
1980
- 1980-04-18 JP JP5025380A patent/JPS56147043A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS56147043A (en) | 1981-11-14 |
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