JPS6230375B2 - - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to an electrophoresis device used in an automatic analyzer for serum proteins, etc.

Normally, in an analyzer for analyzing serum proteins using electrophoresis, a sample (serum) is applied to a support such as a cellulose acetate membrane impregnated with a buffer solution, and then electrophoresis is performed in an electrophoresis tank. The invention provides an apparatus that automates such electrophoresis.

Hereinafter, the present invention will be explained based on the embodiments shown in the drawings. Reference numeral 1 denotes an underframe, and outer tanks 2 and 3 are provided on the underframe 1, and inside thereof are tanks that are movable vertically and horizontally. A tank body 5 and a vertically movable tank body 6 are provided, and a through hole 2 for overflowing water is provided at the side of the tank bodies 5 and 6.
At the same time, electrodes 4 and 4' are respectively arranged in the tank, and papers 7 and 8 are attached to the inner edges 5' and 6', respectively. A presser bar 10 that is vertically and horizontally movable and whose upper ends are mounted on stay plates 33 and 34, and a vertically movable presser bar 1
1 at a required interval, and by lifting and moving laterally, the support 14 is held down by the presser bars 10, 11.
It is configured so that it can be pressed against and pulled against. Further, bearing plates 12, 13 are provided on the outside of the tank bodies 5, 6 to fix the above-mentioned stay plates 33, 34, while cut grooves 41, 42 and 41', 4 provided in the upper part thereof are provided.
Presser rods 10, 11 are bridged between 2', further supporting rollers 15, 15' and 16 for feeding of the support body 14 are supported, and the belt 1 is connected to the rollers 15, 15'.
7 and 17', and also to a driving worm gear 18 bearing on the underframe 1. moreover,
Overflow pipes 31 and 32 are provided at the bottoms of the outer tanks 2 and 3, and a receiving tank 19 for the buffer liquid flowing out through the overflow pipes 31 and 32 is provided below the overflow pipes 31 and 32. is returned to the storage tank via the

On the other hand, protrusions 20 and 21 with wavy slopes are formed on the other sides of the tank bodies 5 and 6, and a vertical edge 22 is formed on one side of the tank body 6, and the stay plate 33 and 34 are provided with cut receiving edges 23 for making the tank body 6 vertically movable.
will be established. Further, the protrusions 20, 2 of the tank bodies 5, 6
Operating plates 26, 26 having substantially similar wave-shaped slopes 24, 25 are mounted on the bearing plates 12, 13 so as to be slidable in the lateral direction. The drive shaft 28 is provided with a protrusion 27 that engages with each of the actuating plates 26, 26'.

Furthermore, a removable lid 9 is attached to the upper side of the migration tank including the outer tanks 2 and 3, and the recirculated buffer liquid is supplied to the tank body 5 and 6 on the mountain-shaped sloped roof. In order to inject into the body, injection pipes 35 and 36 are provided which are connected to the buffer liquid storage tank by a pipe 37 via an infusion device such as a pump,
In addition, the lid body 9 has an opening 38 for applying a sample in the approximate center thereof, and while the opening 38 is normally biased to a closed state by a spring 39, the opening 38 is not affected by the operation of the sample application device. A shutter 40 is provided which is brought into an open state by interlocking engagement pieces 43.

Based on the above configuration, the operation will be explained next. Now, prior to analysis, the tank bodies 5 and 6 are
As shown in the figure, it is in a lowered position above the outer tanks 2, 3, and the inner edges 5', 6' of the outer tanks 5, 6 and the stay plates 33, 3
A required gap is formed between the presser bars 10 and 11 held by the rollers 15 and 1.
The support 14 can be easily fed into the electrophoresis tank from the side through 5' and 16. next,
When the protrusion 27 rotates together with the drive shaft 28 and the actuating plates 26, 26' move in the horizontal direction of the arrow, the protrusions 20, 21 opposite to the slopes 24, 25 respond, and the side that engages with the receiving edge 23 responds. The tank body 6 with the edge 22 rises in the vertical direction of the arrow, but following the rise, the tank body 5 moves in the horizontal direction of the arrow as shown in FIG. The supports 14 placed on the edges 5', 6' can be pressed against the presser bars 10, 11 with appropriate pressure and at the same time be pulled left and right to create tension.

By configuring the present invention as described above, when electrophoresis is performed on the serum on the support 14 by applying current to the electrodes 4 and 4', the tank bodies 5 and 6 are
upper support 1 due to the rise and lateral movement of
By pulling 4 and pressing it against the presser bars 10 and 11, the adhesion to the papers 7 and 8 is improved, and good analysis results can be obtained. In addition, the tank body 5,
The buffer liquid in the tank 6 enters the receiving tank 19 through the overflowing outer tanks 2 and 3, is mixed in one storage tank, and the liquid is transferred to the tank 5, 6 again by the infusion device.
6, it is possible to equalize the ions in both tanks, and it is also possible to prevent air bubbles from adhering to the electrodes 4, 4', etc., so there is no need to replace the electrodes as in the conventional method. The current flow is always good and a stable electrophoresis effect can be obtained.
Furthermore, the heat generated during electrophoresis can be radiated by the liquid in the injection pipe of the infusion device, and the temperature can also be lowered.

In addition, since the lid 9 attached to the upper side of the migration tank is shaped like a mountain with a slope, even if dew drops form on the lid 9, they will flow into the tank along the slope. , it is possible to prevent water droplets from falling onto the support 14, and further, by disposing the shutter 40 on the lid 9, the support 14 can be prevented from falling onto the support 14.
During electrophoresis after coating the sample through the opening 38 on the sample 4, the shutter 40 closes the opening 38 to prevent the support 14 from drying out and to prevent dust and other particles from entering the tank. It can be prevented.

[Brief explanation of the drawing]

FIG. 1 is a cutaway side view of a main part of an electrophoresis device showing an embodiment of the present invention, FIG. 2 is an exploded perspective view of a main part of a tank moving device, and FIG. 3 is a plan view of a main part of the electrophoresis device. FIG. 4 is a perspective view of the main part of the electrophoresis apparatus. In the figure, 1 is the underframe, 2 and 3 are outer tanks, 4 and 4' are electrodes, 5 and 6 are tank bodies, 7 and 8 are paper, and 15 and 1
5', 16 are rollers, 20, 21 are protrusions, 26,
26' is the operating plate.

Claims (1)

[Scope of Claims] 1. Two tanks are provided on the left and right sides, in which electrodes are arranged and filled with a buffer solution, and a filter paper with one end soaked in the buffer solution is arranged on one side of the tank, and each tank is In an apparatus that performs electrophoresis by placing a support on each of the filter papers located at the center of the body and energizing the electrodes, a protrusion with a slope is formed on the outer side of the tank body, and a protrusion is provided on the protrusion. An electrophoresis device characterized in that operating plates having opposing slopes are slidably disposed, and the tank body is moved vertically and laterally by driving the operating plates laterally. . 2 A stay plate is bridged above the tank body, a cut groove is provided in the stay plate, and a presser bar is placed in the cut groove so as to be positioned above the filter paper arranged on the - side of the tank body. An electrophoresis device according to claim 1.