JPS6235620B2 - - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION This invention relates to liquid chromatographs. More specifically, the first system includes a first electrode tank, a sample introduction part, an electrophoresis detector, and a second electrode tank connected in this order by a capillary tube, a mobile phase solvent supply part, a separation column, and a liquid chromatography system. A second system is connected to a graph detector in this order, and consists of a switching valve and a sampling pipe, and when the sampling pipe is in the first position of the switching valve, it is connected between the first system's detector and the second electrode tank. The first system, the second system, the switching valve, and the sampling tube are integrally connected so that the first system, the second system, the switching valve, and the sampling tube are inserted between the mobile phase solvent supply section of the second system and the separation column at the second position. Regarding liquid chromatograph.

In liquid chromatography, when analyzing complex mixtures such as biological samples and natural product samples, it is necessary to remove unnecessary components from the sample.
It is common to perform some preprocessing.

One method for this pretreatment is to prepare the sample for two
There is a method of performing electrophoresis between different electrode tanks and separating only the portion containing the target component, and an apparatus for this purpose has been proposed by the inventors of the present invention (Japanese Patent Laid-Open No. 56-49952 61-2903]).

However, in the past, pretreatment by electrophoresis and analysis by liquid chromatography were completely different processes, and separate devices had to be operated for each.
It was very troublesome to operate.

The present invention solves the above-mentioned problems and provides a liquid chromatograph that can perform pretreatment of a sample by electrophoresis and analysis by liquid chromatography in succession by simply switching a valve.

Hereinafter, the present invention will be explained in detail based on embodiments shown in the drawings.

〓〓〓〓
1 is an embodiment of a liquid chromatograph of the present invention, which includes a first system 2 that pre-processes a sample by electrophoresis, a second system 3 that performs analysis by liquid chromatography, a switching valve 4, It consists of a sampling tube 5.

The first system 2 includes a terminal electrode tank 6, a sample introduction part 7, an electrophoresis detector 9, and a leading electrode tank 1.
0 are sequentially connected by a capillary reach tube 11, and the detector 9 and the leading electrode tank 10
The switching valve 4 is interposed in the flow path 11a between the two. By switching the switching valve 4, this flow path 11a is directly connected, or this flow path 11a is
The sampling tube 5 is inserted into a. 12 is a terminal electrolyte tank, 13 and 14 are stop valves, 15 is a leading electrolyte tank, and 16 is a constant current high voltage power source.

The second system 3 includes a mobile phase solvent supply section 19 consisting of a mobile phase solvent tank 17 and a liquid feeding pump 18, a separation column 20, and a liquid chromatography detector 21 connected in this order. The switching valve 4 is interposed in the flow path 22 between the mobile phase solvent supply section 19 and the separation column 20. By switching the switching valve 4, this flow path 22 is directly connected, or the sampling tube 5 is inserted into this flow path 22. 23 is waste liquid.

The switching valve 4 is a hexagonal valve with connection ports A to F on six sides, and has a structure that can take two positions.The first position is between A, F, B, C, and DE (the solid line ) is conductive, and in the second position A,
Only B, C, D, and EF (dotted line) are electrically connected. The flow path 11a of the first system is connected to connection ports A and B, the flow path 22 of the second system is connected to connection ports D and E, and the sampling tube 5 is connected to connection ports C and F. . Therefore, as described above, the sampling tube 5 is inserted into the flow path 11a of the first system at the first position, and is inserted into the flow path 22 of the second system at the second position.

Next, to explain the operation of this apparatus 1, prior to analysis, the switching valve 4 is set to the first position, the flow path from the terminal electrolyte tank 12 to the sample introduction part 7 is filled with terminal electrolyte, and the sample is The channel from the introduction part 7 to the leading electrolyte tank 15 is filled with the leading electrolyte. Needless to say,
At this time, the sampling tube is included in the latter flow path. On the other hand, in the second system 3, the mobile phase solvent is transferred from the mobile phase solvent tank 17 to the detector 2 by the liquid feeding pump 18.
Let it flow toward 1.

In this state, insert the sample from the sample injection port 8 into the introduction section 7.
A constant current is passed between the terminal electrode cell 6 and the leading electrolytic cell 10 by a constant current power supply 16.

Then, the ion components in the sample are sandwiched between the leading ion and the terminal ion and migrate to the leading electrolytic cell 10, and each component is gradually separated, so while monitoring this with the detector 9,
When the target component enters the sampling tube 5, the switching valve 4 is switched to the second position.

By this switching, the sampling tube 5 is incorporated into the second system 3, but in other words, only the target component in the sample is subjected to liquid chromatography.

As explained above, according to the liquid chromatograph of the present invention, the pretreatment for extracting the target component from a sample containing a complex mixture of components and the analysis of the target component can be easily performed online, making it extremely convenient for operation. preferable.

Furthermore, since the pretreatment step and the analysis step can be performed substantially continuously, there are fewer chances for errors to be introduced, and the analysis time can also be shortened.

It should be noted that the present invention is not limited to the above-mentioned embodiments, and may be modified as desired, for example, by configuring the switching valve with an electromagnetic valve.

[Brief explanation of the drawing]

FIG. 1 is an explanatory diagram of the configuration of an embodiment of the liquid chromatograph of the present invention. 1...Liquid chromatograph, 2...1st system, 3
...Second system, 4...Switching valve, 5...Sampling tube, 6...Terminal electrode tank, 7...Sample introduction section, 9...Detector for electrophoresis, 10...Reading electrode tank, 11... …Capillar Reach Youtube,
19... Mobile phase solvent supply section, 20... Separation column, 21... Liquid chromatography detector. 〓〓〓〓

Claims (1)

[Claims] 1. A first system in which the first electrode tank, the sample introduction part, the electrophoresis detector, and the second electrode tank are connected in this order by a capillary tube, the transfer solvent supply part, the separation column, and the detection for liquid chromatography. a second system connected to the detector in this order, consisting of a switching valve and a sampling pipe, the sampling pipe being inserted between the detector of the first system and the second electrode tank in the first position of the switching valve; The first system and the second system are interposed between the mobile phase solvent supply section of the second system and the separation column to form a liquid chromatography channel at a second position. A liquid chromatograph consisting of a switching valve, a sampling tube, and a sampling tube connected together.