JPS6259085B2 - - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Field of Application] The present invention relates to a fair skin cosmetic containing an extract of animal organs.

[Conventional technology]

BACKGROUND ART Various skin-lightening cosmetics have been provided or researched and developed since ancient times in order to remove spots such as spots and freckles that appear on the skin and to bring about a whitening effect on the entire skin.

In the past, peroxides such as hydrogen peroxide, zinc peroxide, magnesium peroxide, sodium peroxide, and zinc perborate were incorporated into cosmetics, but since these peroxides are extremely unstable substances, It has problems in storage stability and incorporation into cosmetics, and its skin whitening effect is not sufficient. After that, cosmetics containing vitamin C, cysteine, colloidal sulfur, etc., came into use, but they were still not fully satisfactory.

Recently, kojic acid, which is obtained by culturing bacteria such as Aspergillus aspergillus, is effective in whitening the skin as a whitening ingredient in these skin-lightening cosmetics, and skin-whitening cosmetics using kojic acid are known. For example, the
No. 18569, Japanese Patent Application Laid-open No. 53-3538. Further, derivatives of kojic acid have been studied, and cosmetics containing kojic acid derivatives are also known. For example, JP-A-56-7710
Cosmetics containing pyrone compounds, chromone compounds, and flavonol compounds are also known. For example, JP-A-53-3538, JP-A-55-92305, JP-A-55-111410.
No. 55-111411, No. 55-143908, No. 55-
No. 157580, No. 57-14517, No. 57-35506, No. 58
- Publication No. 131911, etc.

Cosmetics containing placenta extracts have also been disclosed. For example, Japanese Patent Publication No. 35-15399.

[Problem that the invention seeks to solve]

Among the various skin-lightening cosmetics disclosed above, cosmetics that have a skin-whitening effect greater than that of conventional skin-lightening cosmetics are created by combining various animal organ components in order to further enhance the skin-whitening effect of placenta extract, which is an extract of animal organs. The purpose of this is to provide a fee.

[Means for solving problems]

The present invention is a cosmetic that exhibits an extremely excellent skin-whitening effect by containing a placenta extract and an animal liver extract.

The placenta extract used in the present invention is obtained by washing the placenta of a human or an animal such as a cow, removing blood, crushing and freezing it, extracting the water-soluble part with water, and then removing impurities by filtration or dialysis. It is an aqueous solution.

The placenta extract used in the present invention is generally commercially available as a placenta extract and is mainly used as a raw material for cosmetics, and may be of human origin,
Although any extract derived from animals such as cows may be used, a placenta extract derived from humans is preferred.

The animal liver extract used in the present invention is obtained by grinding and extracting the liver of an animal, and is obtained by washing the liver of mammals such as cows, pigs, sheep, and goats, and birds such as chickens with water to remove blood, and then grinding the liver. The mixture is made into a porridge, and 2 to 3 times the amount of an extraction solvent such as water is added thereto. After stirring for several hours, a protein removal operation is performed, and an extract is obtained through a filtration process. In this case, water is suitable as the extraction solvent.

Next, an example of producing a liver extract used in the present invention will be given.

Production example 1 Grind 1 kg of pig liver, add water from Step 2, stir and extract for 3 hours, then add lactic acid to adjust the pH to 4.5.
The temperature was adjusted back and forth, and the temperature was heated to 80°C or higher to denature the protein, which was filtered together with the tissue layer. The filtrate was further filtered using diatomaceous earth to obtain extract 2.5. The obtained extract has a specific gravity of 1.003-1.008 and a pH of 4.5-
5.5, evaporation residue 2.0g/100ml or less, total nitrogen 90~
150mg/100ml, nitroprusside reaction and diazo reaction are both positive.

Production Example 2 Add 20 kg of purified water to 10 kg of chicken liver, crush the tissue with a mixer, etc., and stir and extract at 80 to 90°C for 3 hours. Next, adjust the pH to 4.5 with 10% sulfuric acid, and remove proteins and tissue fragments by filtering with cloth. Furthermore, the microparticles are centrifuged or filtered through Celite or the like to obtain an extract.

Next, adjust the pH to 2.0 and warm it to around 50℃, then gradually add 20g of cuprous oxide and stir gently for 2 to 3 hours. Copper salts such as amino acids will precipitate, so centrifuge the mixture. and collect it. This copper salt is washed several times with cold purified water, and hydrogen sulfide gas is blown into the suspension in the purified water in step 5 to precipitate copper as copper sulfide. Excess hydrogen sulfide can be removed by concentrating the liquid filtered through a filter paper under reduced pressure until the volume is reduced to about 1/5. This extract is usually adjusted to a final concentration of 20% and used in cosmetics.

The proportion of placenta extract and liver extract mentioned above is 10 parts placenta extract (by weight) to 5 parts liver extract.
0.01 (weight) is suitable.

The proportion of these active ingredients in cosmetics can be sufficiently effective by containing 0.1 to 10% (by weight) of placenta extract and 0.01 to 5% (by weight) of liver extract. .

The cosmetics of the present invention mainly include lotions, creams,
These are skin cosmetics such as milky lotions and packs, and are made by adding placenta extract and liver extract to cosmetic bases, auxiliaries, etc. that are normally used in these cosmetics.

For example, in lotions, moisturizers such as glycerin and propylene glycol, skin nutrients, etc. are dissolved in purified water, preservatives, fragrances, etc. are dissolved in alcohol, and the two are mixed and solubilized at room temperature. In the production of a lotion, placenta extract and liver extract, which are the active ingredients of the present invention, are added to the water-soluble part to prepare a lotion.

In creams, hydrophilic ingredients such as humectants such as glycerin and sorbitol are added to purified water to form the aqueous phase, and the oil phase consists of beeswax, paraffin, etc.
Microcrystalline wax, ceresin, higher fatty acids, solid oils such as hydrogenated oils, semi-solid oils such as vaseline, lanolin, glyceride, liquid oils such as squalane, liquid paraffin, various ester oils, preservatives, surfactants, etc. Prepare by adding oily ingredients. In the production of general cream, the aqueous phase obtained in this way is heated, and while gently stirring, the oil phase heated to the same temperature is gradually added and emulsified to form cream. Placenta extract and liver extract, which are the active ingredients of the present invention, are added to the aqueous phase to form a cream.

For emulsions, moisturizers such as glycerin, acidic or alkaline PH adjusters, etc. are added to purified water, heated and mixed, ethanol is added to form the aqueous phase, solid oils such as beeswax and paraffin, and semi-containing substances such as vaseline and lanolin are added. Oily components such as preservatives and surfactants are added to liquid oils such as solid oils, squalane, liquid paraffin, and various ester oils, mixed and heated to form an oil phase, and the oil phase is added to the water phase. In the production of general emulsions, in which a protective colloid such as carboxyvinyl polymer or carboxymethyl cellulose is added to the preliminary emulsification and then homogeneously emulsified using a homomixer, the active ingredient of the present invention is added to the aqueous phase. Placenta extract and liver extract are added to the aqueous phase to form a milky lotion.

In Packaging, moisturizers such as glycerin, film agents such as polyvinyl alcohol, and Veegum are added to purified water to swell it, and if necessary, powders such as kaolin, talc, and zinc oxide are added, and fragrances and preservatives are added. In the production of general packs, in which ethanol in which eg.

Next, examples of the present invention will be given.

Example 1 <Cream> Liver extract 1.0% Placenta extract 5.0% Polyoxyethylene stearyl ether 2.09% Polyoxyethylene cetyl ether 2.91% Beeswax 4.0% Setanol 3.0% Isopropyl palmitate 2.0% Liquid paraffin 15.0% Polyethylene glycol monostearate 0.5 % Purified water 64.4% Methyl paraoxybenzoate 0.1% Example 2 <Emulsion> Liver extract 2.0% Placenta extract 4.0% Self-emulsifying glycerol monostearate 1.11% Polyoxyethylene cetyl ether 1.89% MC stearic acid 2.0% Setanol 1.0% Isopropyl myristate 2.0% Purified water 85.90% Paraoxybenzoic acid 0.1% Fragrance Trace example 3 <Pack> Liver extract 0.6% Placenta extract 6.0% Ethanol 3.0% Methyl paraoxybenzoate 0.1% Carboxyvinyl polymer 1.0% Calcium carbonate 0.3% Titanium dioxide 0.02% Purified water 88.98% Fragrance Trace amount example 4 <Lotion> Liver extract 3.0% Placenta extract 10.0% Ethanol 10.0% Methyl paraoxybenzoate 0.1% Citric acid 0.2% Fragrance Trace amount purified water 76.7% [Invention Effect] The skin whitening effect obtained by using the placenta extract and liver extract, which are the active ingredients of the present invention, is synergistically superior to the same effect obtained by using each ingredient alone.

The above effects will be clarified through the following experiment.

Experimental example: In a test tube, 1 ml of L-dopa solution (10mM), 0.9 ml of Matsukulbane's buffer solution (PH6.8), and sample 1.
After adding 0.1 ml of tyrosinase solution (11000G supernatant of B16 mouse melanoma) and stirring well, immediately set it on a spectrophotometer and measure the absorbance at 475 nm over time. was measured.

The samples are placenta extract, liver extract, placenta extract, a liquid containing 1/2% by weight of liver extract, and water as a control. Note that the inhibition rate is the percentage of inhibition of dopachrome production after 8 minutes when the control is set as 100.

The above measurement results are shown in the attached drawings. As is clear from the drawing, the combination of liver extract and placenta extract is
It can be seen that tyrosinase activity is inhibited more synergistically than each alone.

Although it is known that the liver extract and placenta extract, which are the active ingredients of the present invention, have the effect of inhibiting melanin production even when used alone, the present inventor's research revealed that the B16 cells used in this experiment As a result of analyzing the mechanism of inhibiting melanin production in a cell system using In contrast, the placenta extract did not show chelation of copper ions, demonstrating that the mechanism of melanin production suppression by the placenta extract is different from that of the liver extract. As a result, it is thought that a synergistic effect of the liver extract and placenta extract can be obtained due to their different melanin production inhibiting effects.

[Brief explanation of the drawing]

The figure shows the dopachrome production of the active ingredient of the invention.

Claims (1)

[Scope of Claims] 1. A fair-skinned cosmetic characterized by containing a placenta extract and a liver extract. 2. A fair-skinned cosmetic according to claim 1, wherein the placenta extract is a human placenta extract. 3. The fair skin cosmetic according to claim 1, wherein the liver extract is an aqueous liver extract of a mammal or bird.