JPS6333658B2 - - Google Patents

Description

Detailed Description of the Invention Recently, automatic biochemical analyzers that automatically analyze the content of biochemical substances in blood or their activity values using two-wavelength spectrometry have become popular. The majority of methods for obtaining this are those using interference filters. In general, using an interference filter makes it easy to create an optical system for each inspection item, but as the number of inspection items increases, the number of optical elements increases proportionally, making it difficult to integrate the optical system all at once, resulting in drawbacks. There are also quite a few. In addition to this, there are many other problems such as the need to prepare as many interference filters as the number of wavelengths required for measurement, the wavelength accuracy and wavelength purity of the interference filters, and the transmittance.

The present invention relates to an optical system necessary for two-wavelength spectrometry, and by introducing light from the spectral plane and using a spectrometer generally used to select two wavelengths in the opposite direction, each optical element can be efficiently adjusted. In addition to increasing the freedom of wavelength selection, optical
This greatly improves the signal-to-noise ratio. That is, in the normal usage of a spectrometer, if the light incidence point is placed at the desired wavelength position on the spectrum surface and light is incident, light of the desired wavelength will come out from the input slit position of the spectrometer. By sequentially opening the light incident points on the surface, it is possible to sequentially extract light of the target wavelength, and all of the extracted light of each wavelength passes through the same optical path. Since two wavelength spectroscopy requires light of two different wavelengths for each analysis item, the group of light of one wavelength for each item is set as group A, and the group of light of the other wavelength is set as group B. The present invention splits the luminous flux of a white light source into two, guides one luminous flux to a light incidence point placed at the wavelength position of the light of group A on the spectral plane of a spectrometer, and directs the other luminous flux to the wavelength position of group B of light on the same spectral plane. the two beams are alternately and intermittently emitted,
The image of each light incident point on the spectral plane is formed on the line of the incident slit in the normal use of a spectrometer, and behind each of these images a sample cell corresponding to the analysis item is placed. The present invention provides a two-wavelength multi-item automatic analyzer in which light of different wavelengths is alternately taken out and made incident on a sample cell. The present invention will be explained below with reference to Examples.

Figure 1a shows a light source device, where 1 is a self-color light source or other light source with an appropriate spectrum, 2 and 2' are concave mirrors arranged symmetrically with respect to the device center line X-X, and reflect the light from light source 1. The central ray forms two parallel light beams F and F'. 4 is Chiyotsupa and axis X
It rotates around -X and alternately interrupts the light beams F and F'. 5, 5' are optical fibers, 3,
3' is a lens system that converges the light beams F and F' into the end face of the optical fiber as a light beam of uniform brightness.

FIG. 1b shows the input and output parts of the spectrometer. In this figure, the direction of light dispersion is perpendicular to the plane of the figure. 11 is a wavelength selection plate, the position in the X direction corresponds to the analysis item, and the Y direction is the wavelength direction. Small holes 11A and 11B are bored at two desired wavelength positions for each analysis item, and the optical fiber 5 shown in FIG.
The other end of the optical fiber 5' is divided into 6', 7', etc., which are inserted and fixed into the small hole 11A, and the other end of the optical fiber 5' is divided into 6', 7', and so on, which are inserted and fixed into the small hole 11B. 10 is a diffraction grating, 8 is a camera mirror in the normal use of a spectrometer (hereinafter, it will not be stated that each spectrometer is used in a normal use), and the other end of the optical fiber 5, 5' is 6, 7... and 6', The beams emitted from the beams 7' are reflected, and each beam is made into a parallel beam and incident on the diffraction grating 10. The light diffracted by the diffraction grating 10 is directed to the slit 1 by the collimator mirror which is hidden behind the camera mirror 8 in FIG. 1b.
2 on a straight line. At this time, the light emitted from the optical fiber ends 6, 6' located at the left end of the wavelength selection plate 11 is imaged alternately on the right end α position on the slit 12, and the wavelength selection plate 11
The light emitted from the light entrance holes 11A' and 11B' at the right end of the optical fiber 1 (of course, the divided ends of the optical fibers 5 and 5' are inserted and fixed, and is emitted from the end face thereof) is transmitted to the left end of the slit 12. The images are formed alternately. 13 is a self-occurring lens (imaging optical fiber) placed at the image position of the small hole corresponding to each analysis item on the wavelength selection plate 11; 14 is a flow cell for each analysis item placed thereafter; 15 is a photodetector.

FIG. 2 is a plan view of the above-mentioned spectrometer, in which the arrow x in the wavelength selection plate 11 is in the y direction in FIG. 9 is a collimator mirror.

FIG. 3 shows another embodiment of the light source device, in which the chopper 4 is a sector mirror, and the light from the light source 1 is time-divisionally divided into two beams F and F'. Other parts corresponding to those in Figure 1a are given the same reference numerals, so
Each explanation will be omitted.

Figure 4 shows an example of the arrangement of the flow cell 14. The inner diameter of the cell is 2 mm, the cell interval is 5 mm, and if there are 10 analysis items, the total length of the cell arrangement is 45 mm, and if there are 20 items, the total length of the cell arrangement is 95 mm. It is long enough to be covered with one spectrometer. Rather than transmitting light diametrically, this cell transmits light axially, with an optical path length of 10 mm.

The present invention has the above-mentioned configuration and is capable of handling light of various wavelengths at the same time with one spectrometer, and has the feature that the more wavelengths there are, the more effective it becomes. It is more efficient than the conventional device using , and has the advantage that the number of optical elements does not increase even if there are many types of wavelengths. In addition, the light incident points placed on the spectral plane of the spectrometer are arranged two-dimensionally, but since each incident point is guided by an optical fiber from the light source, the light from the light source is arranged on a two-dimensional surface. It has a simpler structure, has a higher light utilization rate, and can easily change the position of the light incident point, compared to the case where the light is irradiated uniformly.

[Brief explanation of the drawing]

FIG. 1a is a plan view of a light source section in one embodiment of the present invention, FIG. 1b is a side view of the spectrometer section, FIG. 2 is a plan view of the spectrometer, and FIG. 3 is another embodiment. FIG. 4 is a plan view of the light source section of FIG. 4, and FIG. 4 is a front view of an example of the arrangement of flow cells. In the figure, 1... light source, 2, 2'... condensing mirror,
3,3'...Lens, 4...Chiyotsupa, 5,
5'... A collection of optical fibers of λ and λ' pairs, 6, 6', 7, 7'... An example of a pair of fibers each selecting two wavelengths, 8 is a camera mirror, 9... Collimator Mirror, 10... grating, 11... wavelength selection plate, 12... slit,
13... Self-cleanse or fiber, 14... Flow cell, 15... Detector.

Claims (1)

[Claims] 1. If an output slit is placed at the input slit position in the normal use of a spectrometer, and light is incident from the output slit, the spectral plane formed will have a direction perpendicular to the dispersion of the light. (in the height direction), determine the height positions corresponding to the analysis items, set the light incidence points at the wavelength positions of the two types of light used in the corresponding analysis items at these height positions, and place these on the above-mentioned output slit. a spectroscope configured to form an image of the light incident point; one end of the spectrometer is positioned at each point corresponding to the light of one wavelength used for each analysis item among the light incident points; One end of the optical fiber bundle 5 gathered in one place is located at each point corresponding to the light of the other wavelength used in each analysis item among the light incident points, and each other end of the optical fiber bundle 5 is located at each point corresponding to the light of the other wavelength used in each analysis item. The optical fiber bundle 5' converges in one place apart from the optical fiber bundle 5', and the light from the light source is divided into two beams, and these two beams are alternately interrupted, and one of the two beams is transmitted to the optical fiber bundle 5'. a light source device that makes the light of the other light beam enter the converging end of the optical fiber bundle 5', and a height above the output slit corresponding to each analysis item on the spectral plane; A two-wavelength multi-item spectroscopic analysis device comprising an optical system that guides light arriving at a point conjugate to a position to a sample cell for a corresponding analysis item.