JPS633591B2 - - Google Patents

Description

BACKGROUND OF THE INVENTION Technical Field The present invention relates to a lid for a culture device. More specifically, the present invention relates to a lid of a culture device for sealing or anaerobically culturing microorganisms or cells.

Prior Art and its Problems There are two types of microorganism or cell culture: culture conducted in an open system and culture conducted in a closed system as a hermetically sealed or anaerobic culture.

Conventionally, when culturing in a sealed system, a lid is placed over the main body of a shear dish with culture recesses or a multi-hole plate with multiple culture recesses, and adhesive tape is applied to the outer periphery of the side surface to seal the gap. It is done by sealing it with tape to make it airtight, or by putting the main body in another sealable container to make it airtight.

However, in the former case, if the culture medium stored in the recess is liquid, the culture medium will spill out if the main body is not held horizontally, so it is difficult to apply adhesive tape to the outer periphery of the side surface, which requires skill. There is. Moreover, in the latter case, the work is not easy.

On the other hand, especially when using a multi-well plate as the main body, an adhesive sheet is directly adhered onto the culture recesses or culture holes called wells to seal the top of each well.

However, in this case, since the adhesive sheet is located above the well and the adhesive faces the culture medium, there is a risk that the culture will be affected by the adhesive. Additionally, if the top surface of the well wall is wet or the width of the top surface is narrow, it will be difficult to completely seal the well, and since culture is performed under high humidity conditions, some of the adhesive sheet may peel off during culture. .

OBJECTS OF THE INVENTION The present invention was made in view of the above-mentioned circumstances, and its main purpose is to provide a lid for a culture device that eliminates the above-described drawbacks and inconveniences. More specifically, it is an object of the present invention to provide a lid for a culture device that has a simple structure and can be sealed extremely easily and reliably.

The present inventor has completed the present invention as a result of repeated studies regarding such an objective.

That is, the present invention has a main body having at least one recess for culturing microorganisms or cells, and a lid covering the main body, and an elastic body is provided at a position of the lid in contact with the upper periphery of the recess of the main body. Further, an elastic ring is provided on the outer side surface of the main body or the inner side surface of the lid, so that the main body and the lid are airtightly engaged via the elastic ring, The culture device is characterized in that the culture device is configured such that the upper part of the recess can be sealed when the lid is engaged with the lid.

Embodiments of the present invention relate to specific structures that more effectively achieve the above objects, and are as follows.

The lid of the culture device of the present invention, wherein the elastic body is plate-shaped and has a shape and size corresponding to the upper periphery of the recess of the main body.

A lid for a culture device according to the present invention, wherein the elastic body has a ring shape corresponding to the upper periphery of the recess of the main body.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described in detail below with reference to embodiments shown in FIGS. 1 to 3 and 4.

As shown in FIGS. 3 and 4, the lid 1 of the culture device of the present invention covers a main body 5 having at least one recess 6 for culturing microorganisms or cells.

In this case, the main body 5 may be in the shape of a shear dish having a culture recess, or, as shown in the figure, may be in the form of a multi-hole plate having a large number of culture recesses 6, ie, wells. And its structure, shape,
Various materials can be used.

An elastic body is provided on the inner surface 15 of the lid 1 that comes into contact with the upper peripheral edge of each recess of the main body 5.

There are various ways to provide the elastic body on the inner surface 15 of the lid 1, and the plate-shaped elastic body may be provided over almost the entire inner surface. As shown in the figure, more preferably, a plate-shaped elastic body 25 having a shape corresponding to the upper periphery of the recess 6 of the main body 5 and dimensions slightly larger than the upper periphery of the recess 6 is provided. It is preferable to provide it corresponding to the recess 6. At this time, the upper part of each corresponding recess 6 connects the lid 1 to the main body 5.
When it is placed over the body, it is sealed by the plate-like elastic body 25, as shown in FIG.

Further, as shown in FIGS. 1 to 3, the elastic body is preferably a ring-shaped elastic body 21 having a shape corresponding to the upper peripheral edge of the corresponding recess 6, and the outer edge thereof A more favorable result can be obtained if it is made larger than the circumferential edge and its inner edge is made slightly smaller than the upper circumferential edge of the corresponding recess 6.

At this time, the sealing of each recess 6 is ensured, and the interior of the recess 6 can be observed from the outside by making the lid 1 transparent. In such a case, by covering the main body 5 with the lid 1, each corresponding recess 6 is sealed by the ring-shaped elastic body 21 and the inner surface 15 of the lid.

Note that this preferably plate-shaped, ring-shaped, etc. elastic body can be made of natural or synthetic rubber, silicone rubber, etc., and among these, silicone rubber is most suitable.

Further, its thickness may be approximately 0.5 to 2 mm.

In order to provide such an elastic body on the inner surface of the lid 1, it can be bonded with an adhesive or the like, or the lid 1 itself can be used as an elastic body and a ring-shaped or plate-shaped elastic body 21,
25 may be integrally molded with the lid body 1.

As shown in FIGS. 3 and 4, such a lid body 1 is placed over the main body 5 and fixed together, and the upper part of each recess 6 is sealed with an elastic body.

In this case, for example, as shown in FIG.
The elastic ring 7 is attached to the outer side surface of the main body 5 or the lid 1.
It is preferable that the main body and the lid be airtightly engaged with each other via the elastic ring 7. In this way, no separate fixing device is required.

In such a case, the elastic ring 7 may be provided on at least one of the outer side surface of the main body 5 and the inner side surface of the lid 1.

To provide the elastic ring on the outer side surface of the main body or the inner side surface of the lid body, for example, as shown in FIG. 3, a groove is formed on the surface where the elastic ring is to be provided, and the elastic ring is Manufacturing is easy if the parts are fitted.

Note that such an elastic ring may also be made of the same material as described above.

Specific Effects of the Invention The lid 1 of the culture device of the present invention is used by storing a culture medium in the recess 6 and placing it over the main body 5 containing microorganisms or cells, thereby fixing and sealing both.

At this time, the corresponding recesses 6 are sealed by the elastic bodies 21 and 25, and the sealing is performed reliably and well, and the lid 1 and the main body 5 are connected to the inner surface of the side of the lid or the outer surface of the side of the main body. The elastic ring 7 provided provides an airtight engagement. In this case, unlike when using an adhesive sheet, the seal will not be broken during culture. In addition, since the upper part of the recess 6 is sealed,
When fixing the lid 1 and the main body 6, even if they are tilted, the culture medium will not spill or mix with other culture media.

Furthermore, unlike when using adhesive tape, there is no adverse effect on culture.

Such an effect is achieved by the elastic bodies 21 and 25.
This can be achieved with an extremely simple configuration, in which the device is provided on the inner surface of the lid body 1.

Further, the engagement and removal of the lid 1 and the main body 5 via the elastic ring 7 is extremely easy, and the operability is excellent.

Furthermore, the structure is simple, and the product has excellent durability and productivity. Furthermore, since the elastic ring 7 is used, there are no restrictions on the shape of the main body.

In this case, according to the above embodiment, if the elastic body is made into a plate shape corresponding to the upper peripheral edge of the recess 6,
The reliability of sealing is improved.

Further, according to the above-described embodiment, if the tube is formed into a ring shape, sealing reliability is improved and observation during culturing is facilitated.

[Brief explanation of the drawing]

Figures 1 to 3 are diagrams for explaining an embodiment of the present invention, of which Figure 1 is a perspective view and Figure 2 is a perspective view.
The figure is a cross-sectional view, and FIG. 3 is a cross-sectional view showing the assembled state with the main body. FIG. 4 is a sectional view showing another embodiment of the present invention assembled with the main body. DESCRIPTION OF SYMBOLS 1... Lid body, 15... Inner surface, 21... Ring-shaped elastic body, 25... Plate-shaped elastic body, 5... Main body, 6...
... recess, 7... elastic ring.

Claims (1)

[Scope of Claims] 1. A main body having at least one recess for culturing microorganisms or cells, and a lid covering the main body, the lid at a position in contact with the upper periphery of the recess of the main body. An elastic body is provided, and an elastic ring is provided on the outer side surface of the main body or the inner side surface of the lid body, and the main body and the lid body are configured to be airtightly engaged with each other via the elastic ring, A culture device characterized in that the top of the recess can be sealed when the main body and the lid are engaged. 2. Claim 1, wherein the elastic body is plate-shaped and has a shape and dimensions corresponding to the upper periphery of the recess of the main body.
Culture equipment described in section. 3. The culture device according to claim 1, wherein the elastic body has a ring shape corresponding to the upper peripheral edge of the recess of the main body.