JPS6364745B2 - - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a blood cell washing centrifuge, and more particularly to a blood cell washing centrifuge having a structure suitable for automatically discharging a supernatant.

A blood cell washing centrifuge is generally used to wash red blood cells when determining blood type at the time of blood transfusion, which is called the Coombs test. Put the serum for testing into a test tube, rotate the rotor, and forcefully inject saline with the test tube in a horizontal position to wash the serum (red blood cells) in the test tube, then continue to rotate the rotor. Rotate and centrifuge to sediment red blood cells. Next, discard the upper supernatant, leaving only the red blood cells. Repeat this operation several times to thoroughly wash the red blood cells.

FIG. 1 is a longitudinal sectional view of a conventional blood cell washing centrifuge. In FIG. 1, 1 is a motor, 2 is a drive shaft, and 3 is a rotor. The rotor 3 is connected to the motor 1 via the drive shaft 2, and is rotationally driven by the motor 1. Reference numeral 4 denotes a test tube holder for mounting test tubes 5 arranged in a circular manner on the rotor 3, and a plurality of test tube holders 4 are provided. Reference numeral 6 denotes a distributing element, which is configured to hold the test tube holder 4 so that it does not move beyond a certain angle when the test tube holder 4 moves from the vertical direction to the horizontal direction due to centrifugal force when the rotor 3 rotates. The distribution element 6 rotates together with the rotor 3, and saline water is supplied from the top of the distribution element, and this saline water moves uniformly within the distribution element 6 due to centrifugal force, and is transferred from the nozzle provided in the distribution element 6 to the distribution element 7. Therefore, the saline solution is vigorously injected into the test tube 5 containing the serum, which is attached to the test tube holder 4 held at the position indicated by the two-dot chain line in the figure. This saline solution is mixed with the serum in the test tube 5 to wash the red blood cells. When the rotor 3 continues to rotate in this state, the salt suspension is centrifuged, the red blood cells are centrifuged and sedimented, and a supernatant remains above. Next, the supernatant liquid in the test tube 5 must be discharged to the outside of the test tube 5. In this case, conventionally, the following procedure was used. That is, when the rotor 3 is stopped and the test tube holder 4 returns to the vertical position,
The electromagnetic coil 8 provided at the bottom of the test tube holder 4 is energized to excite the magnetic pole 9 that rotates together with the test tube holder 4, and the test tube holder 4 is attracted by the magnetic pole 9, so that the test tube holder 4 is placed in a vertical position. Try to keep it. In this state, the rotor 3 is rotated again. In this case, since the test tube holder 4 rotates while maintaining its vertical position, the supernatant liquid in the test tube 5 is discharged by centrifugal force and is received by the supernatant receiver 10.

However, in the conventional structure described above, since there is a magnetic circuit, the output of the motor 1 must be increased by that amount, and the electromagnetic coil 8 is required, making the structure complicated and expensive. It has the disadvantage of becoming.

The present invention has been made in view of the above, and its purpose is to hold the test tube holder vertically and release the supernatant liquid in the test tube when the rotor is rotated. To provide a blood cell washing centrifuge which is simple in structure and does not require any electric power.

A feature of the invention is that the means for freeing and restraining the test tube holders in a vertical position are provided by cut-out grooves in the outer periphery of the rotor that rotates integrally with the shaft of the rotor, in which the extensions of each test tube holder engage, respectively. a first disk provided with a shaft, which is rotatable around the shaft whose center of rotation coincides with the first disk, and when the first disk rotates forward, the first disk rotates together with the first disk; The first disk rotates to allow each of the test tube holders to swing, and engages with each of the notched grooves so that when the first disc rotates in the opposite direction, rotation is restrained and the test tube holders do not swing. The present invention is characterized in that the extension portion of each of the test tube holders is configured with a second disc provided with means for locking into each of the notched grooves.

The present invention will be explained in detail below using the embodiments shown in FIGS. 2 and 3. FIG. 2 is a cross-sectional front view of essential parts showing an embodiment of the blood cell washing centrifuge of the present invention.
Components that are the same as those in FIG. 1 are designated by the same reference numerals, and their explanation will be omitted here. In FIG. 2, a first disc 11 rotates integrally with the shaft of the rotor 3, and a second disc that can freely rotate around the shaft whose center of rotation coincides with the first disc 11. The second disc 12 is attached to the housing 13 of the motor 1 via a bearing 14, and its rotation is restrained by a sliding piece 15. FIG. 3 is a structural diagram of the first disc 11 and the second disc 12 in FIG.
The lower half shows a part of the second disk 12. As shown in FIG. 3, the first disc 11 is provided with cutout grooves 17 on its outer periphery in which the extensions 16 of the test tube holder 4 can freely engage. A fold 18 is provided to bring the second disc 12 in phase when the disc 11 rotates in the forward direction. and second disk 12
, the second disc 12 is folded back 18 to the first disc 12.
When the test tube holder 4 rotates in the same phase with the disk 11 in the positive direction (in the direction of arrow A in the figure), the test tube holder 4
The extension part 16 of the first disk 11 does not prevent the test tube holder 4 from moving from the vertical direction to the horizontal direction (referred to as swing in the present invention) by separating from the notch groove 17 of the first disk 11 due to centrifugal force; When the first disc 11 rotates in the opposite direction and the second disc 12 is restrained from rotating by the sliding piece 15 and rotates in the opposite direction with a phase shift from the first disc 11, The extension portion 16 is restrained by the notch groove 17 to prevent the test tube holder 4 from swinging (hereinafter referred to as decant).
A structural cutout groove 19 is provided on the outer periphery.

Therefore, according to the embodiment of the present invention described above, when the rotor 3 rotates forward and the test tube holder 4 also rotates forward, the test tube holder 4 can swing; When the rotor 3 is rotated in the opposite direction with the test tube holder 4 having stopped and returned to the vertical position with its extension 15 engaged in the cutout groove 17 of the first disk 11, the test tube holder 4 4 becomes unable to swing due to the action of the second disk 12, and is decanted in the vertical position, and the supernatant liquid centrifuged in the test tube 5 is released outside the test tube 5 by centrifugal force, and it is Kamisumi receiver 10
You can make it so that you receive it. Moreover, the first
Compared to the case shown in the figure, the structure is simple because it is composed of the first and second disks 11 and 12,
Moreover, since no electromagnetic coil is required, no electric power is required. The sliding piece 15 is provided to provide frictional resistance to the second disc 12, but if the frictional resistance of the bearing 14 itself is sufficient, the sliding piece 15 may not be provided.

As explained above, according to the present invention, when the rotor is rotated, the test tube holder can be held in a vertical position and the supernatant liquid in the test tube can be released, and the structure is simple. Since it does not require any electric power, it has the effect of being highly practical.

[Brief explanation of drawings]

Figure 1 is a longitudinal cross-sectional view of a conventional blood cell washing centrifuge.
FIG. 2 is a sectional front view of essential parts showing an embodiment of the centrifuge for washing blood cells according to the present invention, and FIG. 3 is a structural diagram of the first and second disks shown in FIG. 2. 1... Motor, 3... Rotor, 4... Test holder, 6... Distribution element, 7... Nozzle, 10...
Supernatant receiver, 11...first disc, 12...second disc, 14...bearing, 16...extension of test tube holder, 17, 19...notch groove, 18...
Turn around.

Claims (1)

[Scope of Claims] 1. A motor, a rotor that is connected to the motor and rotates, and a rotor that is arranged in a circular shape on the rotor and is rotatable, and when the rotor rotates, it rotates from the vertical direction to the horizontal direction due to centrifugal force. A device comprising a plurality of test tube holders and a dispensing element for injecting blood cell washing liquid into test tubes attached to each test tube holder when the rotor rotates, the device rotating integrally with the shaft of the rotor. a first disk whose outer periphery is provided with notched grooves in which the extensions of the test tube holders are respectively engaged; When the first disc rotates in the forward direction, it rotates together with the first disc to allow each of the test tube holders to swing;
means for locking the extensions of the test tube holders that are engaged with the notch grooves so that the test tube holders do not swing when the first disk rotates in the opposite direction; A centrifugal machine for washing blood cells, characterized in that it is equipped with a second disk. 2. The blood cell washing centrifuge according to claim 1, wherein the first disc is provided with means for rotating the second disc in the same phase when the first disc rotates in the forward direction.