JPS6367656B2 - - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a plastic container for blood testing.
Specifically, the present invention relates to a so-called spittoon, a plastic tubular container with a bottom used for separating serum and plasma from a whole blood sample of a subject by centrifugation. In recent years, coupled with the remarkable progress in inspection technology,
Serum tests such as serum biochemistry tests, serum immunology tests, and hematology tests have become widely used and have greatly contributed to disease prevention and early diagnosis. Serological tests are the main body of blood testing, and the serum required for testing is usually obtained by coagulating a whole blood sample collected in a spittoon and then separating it from blood clots with different specific gravities by centrifugation. Traditionally, spitz made of glass have been used, but glass spitz are heavy and
In recent years, spits made of synthetic resins such as polystyrene, polymethyl methacrylate, and polyethylene have been widely used because they have drawbacks such as being easily damaged during storage and transportation of whole blood samples and during centrifugation operations. This type of synthetic resin spittoon is difficult to damage,
Although it has the advantage of being easy to incinerate, it takes a significantly longer time for blood to coagulate compared to glass spittoons, so after blood is collected from a subject,
Serum could not be obtained quickly. Furthermore, when a synthetic resin spittoon is used, the contractility of the blood clot components produced is poor, and as a result, a large amount of serum is incorporated into the blood clot, resulting in a small yield of serum.
There was a problem that blood clots were often mixed into the collected serum. In order to solve the above problem, the present inventors conducted intensive research on the influence of the Spitz's inner wall surface on blood coagulation, and as a result, they decided to make unsaturated fatty acid amide compounds and silicon oxide fine particles exist on the inner wall surface. Therefore, the inventors have discovered that it is possible to shorten the time required for blood coagulation and also to satisfactorily separate serum components and blood clot components, leading to the completion of the present invention. That is, the gist of the present invention is that an unsaturated fatty acid amide compound and fine particles of silicon oxide with an average particle size of 100μ or less are present on the inner wall surface, and the amount used is determined based on the material used for forming the inner wall surface. hand,
0.05-1.0% by weight of unsaturated fatty acid amide compounds,
A plastic container for blood testing characterized by containing 0.01 to 1.0% by weight of silicon oxide. In the present invention, any of thermoplastic synthetic resins, thermosetting synthetic resins, and modified natural resins can be used as the material resin for the container, that is, the spitz. Examples of thermoplastic resins include polystyrene, polymethyl methacrylate, polyvinyl chloride, polyethylene, polypropylene, poly-4-methylpentene-1, polybutene, polyethylene terephthalate, polybutylene terephthalate, styrene-acrylonitrile copolymer, and styrene-anhydride. Maleic acid copolymer, styrene-acrylic acid copolymer, styrene-methyl methacrylate copolymer,
Thermosetting resins such as ethylene-propylene copolymer, polyvinyl alcohol acetal, polyvinyl alcohol butyral, etc. include unsaturated polyester, epoxy resin, epoxy-acrylate resin, etc. Modified natural resins include cellulose acetate, cellulose propionate,
Cellulose acetate butyrate, ethyl cellulose, ethyl chitin, etc. are used, and among them, polyolefins such as polyethylene, polypropylene, ethylene-propylene copolymer, polybutene, and poly-4-methylpentene-1 are preferably used. In the present invention, by using the above-mentioned polyolefin as the spitz material resin, a particularly excellent effect of shortening the blood coagulation time and improving the separability of serum components and blood clot components can be obtained. Further, as the unsaturated fatty acid amide compound in the present invention, any unsaturated fatty acid amide compound can be used as long as it does not have a harmful effect on serum or its test. For example, undecylenic acid amide, olefinic acid amide, elaidic acid amide, cetoleic acid amide, erucic acid amide, etc. are used as the unsaturated fatty acid amide. Specific examples of the silicon oxide in the present invention include silicon dioxide obtained by hydrolyzing silicon tetrachloride in an oxyhydrogen flame, and silicic acid obtained from soluble silicates such as sodium silicate. As the above-mentioned fine particles of silicon oxide, fine particles having an average particle diameter of 100 μm or less, preferably 50 μm or less are used. Furthermore, in the present invention, the presence of the unsaturated fatty acid amide compound and the silicon oxide fine particles on the inner wall surface of the container refers to the case where the unsaturated fatty acid amide compound and the silicon oxide fine particles are present only on the inner wall surface; This refers to both the case where these are present not only on the surface of the inner wall but also in the inner layer of the wall. The plastic container for blood tests of the present invention can be manufactured by various methods. The first method is to uniformly mix an unsaturated fatty acid amide compound and silicon oxide fine particles into a resin as a molding material in advance, and then apply the mixture to injection molding, blow molding, compression molding, transfer molding, vacuum forming, etc. It is molded by an appropriate molding method such as cast molding. According to this method, the unsaturated fatty acid amide compound and silicon oxide fine particles are dispersed throughout the wall of the container not only on the surface but also in the thickness direction. In the case of the above method, the molding material used is one containing an unsaturated fatty acid amide compound in a range of 0.05 to 1.0% by weight and a silicon oxide fine particle in a range of 0.01 to 1.0% by weight. The second method is to form a thin layer or film containing the above-mentioned unsaturated fatty acid amide compound and silicon oxide fine particles on the inner wall surface of the container. This thin layer or film can be formed by applying fine particles of the unsaturated fatty acid amide compound and silicon oxide to the inner wall surface of the container together with a suitable adhesive or solvent. According to this method, when forming a thin layer, the thin layer or film contains an unsaturated fatty acid amide compound in a range of 0.05 to 1.0% by weight and silicon oxide fine particles in a range of 0.01 to 1.0% by weight. Adjust the thin layer or film-forming material to As mentioned above, the plastic container of the present invention has the following features:
Since an unsaturated fatty acid amide compound and fine particles of silicon oxide are present on the inner wall surface of the container, the whole blood sample injected into the container quickly loses blood coagulation factors by contact with the silicon oxide. activated, and the time required for blood clotting is significantly reduced. Furthermore, the presence of unsaturated fatty acid amide compounds on the inner wall surface of the container effectively suppresses the adhesion of cellular components in the blood such as platelets and red blood cells, and formed substances such as fibrin to the inner wall surface. result,
After blood coagulation and centrifugation, the serum supernatant component and blood clot component are completely separated, the blood clot component no longer mixes with the serum component, and the contraction of the blood clot component progresses sufficiently, and the serum The yield increases significantly. Therefore, the container of the present invention can be used for blood test blood collection tubes,
It can be suitably used for blood collection syringes, serum separation containers, etc. that also have the purpose of blood separation. The present invention will be explained below using examples. Examples 1 to 3 and Comparative Examples 1 to 3 Molding materials having the compositions shown in Table 1 were injection molded to obtain bottomed spittoons with an outer diameter of 17 mm, an inner diameter of 15 mm, and a height of 110 mm. For comparison, a composition system that does not contain an unsaturated fatty acid amide compound (referred to as Comparative Example 1) and a composition system that does not contain silicon oxide fine particles (referred to as Comparative Example 2)
were also injection molded in the same manner as above, and a bottomed spittoon with the same dimensions was prepared. Furthermore, for comparison, a commercially available polyethylene spittoon having the same dimensions as above was prepared (referred to as Comparative Example 3). After injecting 5 c.c. of fresh human blood into each spittoon and leaving it at 25℃, the blood clotting time is the time required for the blood clot components separated from the serum components to sufficiently contract and the blood to stop flowing at all. and blood coagulation was evaluated. Next, the mixture was centrifuged at 3000 rpm for 5 minutes, and the state of serum separation was observed, and the supernatant serum was collected with a pipette, and the collected amount was defined as the serum yield. As shown in Table 1, the results are shown in Table 1 together with the results for commercially available polyethylene spitz, the spitz of the present invention has faster blood coagulation and better serum separation compared to commercially available polyethylene spitz. it is obvious. 【table】

Claims (1)

[Claims] 1 An unsaturated fatty acid amide compound and fine particles of silicon oxide with an average particle size of 100μ or less are present on the inner wall surface, and the amount of unsaturated fatty acid amide compound used is determined based on the material used for forming the inner wall surface. Compound: 0.05~1.0% by weight, silicon oxide: 0.01~
A plastic container for blood testing characterized by a concentration of 1.0% by weight.