JPS638757B2 - - Google Patents
Info
- Publication number
- JPS638757B2 JPS638757B2 JP18946283A JP18946283A JPS638757B2 JP S638757 B2 JPS638757 B2 JP S638757B2 JP 18946283 A JP18946283 A JP 18946283A JP 18946283 A JP18946283 A JP 18946283A JP S638757 B2 JPS638757 B2 JP S638757B2
- Authority
- JP
- Japan
- Prior art keywords
- sorbitol
- glucose
- producing
- cryptococcus
- strain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims description 14
- 239000000600 sorbitol Substances 0.000 claims description 14
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 241001337994 Cryptococcus <scale insect> Species 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 2
- 150000002972 pentoses Chemical class 0.000 claims description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 1
- 229910052799 carbon Inorganic materials 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 238000000034 method Methods 0.000 description 5
- 244000005700 microbiome Species 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 239000007868 Raney catalyst Substances 0.000 description 1
- NPXOKRUENSOPAO-UHFFFAOYSA-N Raney nickel Chemical compound [Al].[Ni] NPXOKRUENSOPAO-UHFFFAOYSA-N 0.000 description 1
- 229910000564 Raney nickel Inorganic materials 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 238000010531 catalytic reduction reaction Methods 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000004880 explosion Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000006481 glucose medium Substances 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- PXHVJJICTQNCMI-UHFFFAOYSA-N nickel Substances [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
- 239000000606 toothpaste Substances 0.000 description 1
- 229940034610 toothpaste Drugs 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000000811 xylitol Substances 0.000 description 1
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 1
- 229960002675 xylitol Drugs 0.000 description 1
- 235000010447 xylitol Nutrition 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Description
本発明はブドウ糖から微生物法によりソルビト
ールを製造する方法に関し、より詳しくはクリプ
トコツカス属に属し、ソルビトール生産能を有す
る微生物を用いて、ソルビトールを効率よく製造
する方法に関するものである。
ソルビトールは特有の甘味を有すると共に反応
性に乏しく、無害であつて湿潤調節作用を有する
ため、そのまま食品、歯みがき、化粧品等に添加
物として使用される他、ビタミンＣ、界面活性剤
製造の中間原料として広く使用されている。
ソルビトールは現在、工業的にはブドウ糖をラ
ネーニツケル等のNi触媒を用いて接触還元して
製造されているが、このような方法によれば反応
条件が必然的に高温（160℃）、高圧（170Kg／cm2）
となりエネルギーを多く消費する他、耐圧容器を
必要とし、更に水素を取扱う関係上爆発の危険が
内在している。
本発明者らは上記方法とは発想を異にし、グル
コースを微生物学的に還元することにより緩和な
条件でソルビトールを製造する方法を試みた。ブ
ドウ糖をソルビトールに還元する機構は、動物細
胞系では羊の精のう中（H.G.Hers Biochem.
Biophys.Acta37（1960）127−138）あるいはレン
ズ中（M.Lou Biochem.Biophys.Acta 141
（1967）547−559）にその存在が知られているが、
微生物ではソルビトールを生産した例がなくわず
かに特公昭45−24834号及び46−23038号にキシロ
ースをキシリトールに好気的に発酵して還元する
方法が開示されているにすぎない。また、上記発
明に開示された菌株をグルコース培地中で培養し
てもソルビトールを得ることはできなかつた。
そこで、本発明者らは土壌などからソルビトー
ルの生産能を有する微生物を得る目的でスクリー
ニングを行つた
その結果、五炭糖中で培養増殖せしめたクリプ
トコツカス属の培養体、菌体、あるいは菌体抽出
物、凍結乾燥菌体が基質グルコースをソルビトー
ルに高収率に還元することを見出して本発明を完
成するに至つた。
本発明に用いられる好ましいクリプトコツカス
属菌としては、今回、新しく土壌中より分離した
Ｚ−24株が代表菌として例示されるが、本菌株の
菌学的性質は表１−１および表１−２に示すとお
りである。
The present invention relates to a method for producing sorbitol from glucose by a microbial method, and more specifically to a method for efficiently producing sorbitol using a microorganism belonging to the genus Cryptococcus and capable of producing sorbitol. Sorbitol has a unique sweet taste, has low reactivity, is harmless, and has a moisturizing effect, so it is used as an additive in foods, toothpaste, cosmetics, etc., and is also used as an intermediate raw material in the production of vitamin C and surfactants. It is widely used as Currently, sorbitol is industrially produced by catalytic reduction of glucose using a Ni catalyst such as Raney nickel, but this method requires high temperature (160°C) and high pressure (170 kg). / cm2 )
In addition to consuming a lot of energy, it also requires a pressure-resistant container, and there is an inherent danger of explosion due to the handling of hydrogen. The present inventors differed in concept from the above method and attempted a method for producing sorbitol under mild conditions by microbiologically reducing glucose. The mechanism for reducing glucose to sorbitol has been demonstrated in animal cell systems in the spermatozoa of sheep (HGHers Biochem.
Biophys.Acta37 (1960) 127-138) or in the lens (M.Lou Biochem.Biophys.Acta 141
(1967) 547-559).
There are no examples of microorganisms producing sorbitol, and only Japanese Patent Publications No. 45-24834 and No. 46-23038 disclose a method of aerobically fermenting and reducing xylose to xylitol. Further, even if the strain disclosed in the above invention was cultured in a glucose medium, sorbitol could not be obtained. Therefore, the present inventors conducted a screening for the purpose of obtaining microorganisms capable of producing sorbitol from soil etc. As a result, cultures, cells, or microorganisms of the genus Cryptococcus grown in pentose were found. The inventors have completed the present invention by discovering that a lyophilized bacterial cell extract and freeze-dried bacterial cells reduce the substrate glucose to sorbitol in high yield. A representative example of Cryptococcus genus bacteria used in the present invention is strain Z-24, which was recently isolated from soil.The mycological properties of this strain are shown in Tables 1-1 and 1. -2.
【表】
胞子形成を認めず
[Table] No spore formation observed
Claims (1)
産能を有する菌株を五炭糖類を主炭素源とする培
地に培養し、次いで得られた培養体とブドウ糖を
接触せしめソルビトールを製造することを特徴と
するクリプトコツカス属菌によるソルビトールの
製造法。1. Cryptococcus, which is characterized by culturing a strain of the genus Cryptococcus and having sorbitol-producing ability in a medium containing pentose as the main carbon source, and then contacting the resulting culture with glucose to produce sorbitol. A method for producing sorbitol using Kotsucus bacteria.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP18946283A JPS6083590A (en) | 1983-10-11 | 1983-10-11 | Production of sorbitol by fungus of genus cryptococcus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP18946283A JPS6083590A (en) | 1983-10-11 | 1983-10-11 | Production of sorbitol by fungus of genus cryptococcus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6083590A JPS6083590A (en) | 1985-05-11 |
| JPS638757B2 true JPS638757B2 (en) | 1988-02-24 |
Family
ID=16241669
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP18946283A Granted JPS6083590A (en) | 1983-10-11 | 1983-10-11 | Production of sorbitol by fungus of genus cryptococcus |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6083590A (en) |
-
1983
- 1983-10-11 JP JP18946283A patent/JPS6083590A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6083590A (en) | 1985-05-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Saha et al. | Biotechnological production of mannitol and its applications | |
| EP0120369B1 (en) | Production of acetic acid by a continuous fermentation process | |
| Nigam | Investigation of some factors important for solid-state fermentation of sugar cane bagasse for animal feed production | |
| US3957587A (en) | Production of xylose (dextrose) isomerase enzyme preparations | |
| CN117778479B (en) | A method for preparing 1,2-propylene glycol by microbial fermentation | |
| JPS638757B2 (en) | ||
| JPS5943157B2 (en) | Method for producing sorbitol using Pichia bacteria | |
| JPS639829B2 (en) | ||
| KR101435580B1 (en) | Method for Producing Bacterial Cellulose Using Gluconacetobacter sp. KCG326 Strain Using Optimal Carbon Source and Nitrogen Source | |
| KR930001261B1 (en) | Method for producing citric acid using citrus peel | |
| GB1451020A (en) | Process for producing bacterial cells | |
| JPS639830B2 (en) | ||
| KR20010067882A (en) | Candida magnoliae producing mannitol and its fermentation method for producing mannitol | |
| JPS5934357B2 (en) | Method for producing sorbitol using microorganisms | |
| JPS5937075B2 (en) | Method for producing sorbitol using Hansenula bacteria | |
| US6238895B1 (en) | Production of aspartic and malic acids with microbacterium | |
| US6635455B1 (en) | Process for producing pyrrole-2-carboxylic acid | |
| JPS5930400B2 (en) | Method for producing sorbitol using microorganisms | |
| JPS57110192A (en) | Preparation of l-lactic acid | |
| US3042585A (en) | Fermentation process for producing l-glutamic acid | |
| JPS6035113B2 (en) | Method for producing sorbitol using microorganisms | |
| JPH0795959B2 (en) | Method for producing fructose by glucose-isomerase | |
| JPS5857155B2 (en) | Production method of coenzyme Q↓1↓0 by fermentation method | |
| JPH05137590A (en) | Purification of lactosyl fructoside by microorganism | |
| JPS5642589A (en) | Highly thermophile fumarase, its production and production of l-malic acid utilizing microorganism producing thermophile fumarase and the same |