JPH0117738B2 - - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION (Field of Industrial Application) The present invention relates to a novel deodorizer containing microorganisms as an active ingredient.

(Prior Art) Various deodorizing agents have been known in the past, including adsorption deodorizing agents such as activated carbon that physically adsorb malodorous components, and chemical deodorizers that capture malodorous components by chemically reacting with them. Deodorizers and biological deodorizers that utilize the assimilation effect of microorganisms on bad odors are known.

(Problem to be solved by the invention) Among conventional deodorizing agents, those that utilize the physical adsorption power of activated carbon, etc., cannot be expected to be any more effective once the adsorption of malodorous components is saturated, and a new deodorizing agent is required each time. It is necessary to use it. Further, although chemical deodorizing agents are quick-acting, they are temporary and are not effective for a long period of time, and the deodorizing agents used pose problems such as environmental pollution. Furthermore, biological deodorizers are expected to be slow-acting and permanent, but no microorganisms have yet been found that are selective to malodorous components and have sufficient effects.

Therefore, there is a strong demand for the development of a deodorizing agent that is highly efficient, does not pollute the surrounding area, and is durable, especially in industrial fields that generate large amounts of bad odors, such as livestock farming.

(Means for Solving the Problems) In response to the above-mentioned demands of the industrial world, the present inventor has conducted extensive research to solve the problem of bad odors that occur in large quantities especially in livestock industries such as pig farming and poultry farming. By discovering microorganisms that have the ability to effectively assimilate mercaptan compounds and volatile fatty acids, which are the main components of these bad odors, and using these microorganisms as the active ingredients of deodorizers, we have created highly effective deodorizers that are highly effective and can harm the surrounding environment. The present invention was completed based on the discovery that a deodorizing agent that does not cause staining and is durable can be obtained.

That is, the present invention is a microbial deodorizer containing Alcaligenes odorans and/or Serratia marsetuscens as an active ingredient.

To explain the present invention in detail, Alcaligenes odorans, which is the active ingredient of the deodorizer of the present invention, exhibits oxidase, motility, nitrite reduction, production of yellow pigment, growth on cetrimide agar, and growth on pine conch agar. development,
Lipase (twin 80), growth in acetate medium, DL
-Active (+) in the utilization of norleucine, OF test, nitrate reduction, pigment production of pyfoverdine, urease, phenylalanine deaminase, gelatinase, DNase, growth in penicillin (10u), utilization of pelagonate, It is inactive (-) in grapes (acid), lebulose (acid), maltose (acid), xylose (acid), and mannite (acid).

On the other hand, the other active ingredient, Serratia marcescens, promotes motility,
VP test, active (+) in citric acid, gelatase, lysine decarboxylase, ornithine decarboxylase, DNase, ONPG, carbohydrate (acid), glucose (acid), maltose, sucrose, mannitrate, hydrogen sulfide, phenyl It is inactive (-) in alanine deaminase, arginine dihydrolase, arabinose, lactose, raffinose, rhamnose, and dulsit.

The above-mentioned effective bacteria in the present invention can be obtained by screening as follows.

(1) Alcaligenes odorans: 10 g of sodium acetate, 1 g of dipotassium monohydrogen phosphate, 1 g of ammonium chloride, 200 mg of sodium sulfate, 10 mg of ferrous sulfate, 10 mg of calcium chloride, distilled water.
Prepare a medium consisting of 100 ml with a pH of 7.5,
100 ml of the medium was dispensed into a 300 ml Erlenmeyer flask, inoculated with 1 g of swine manure compost, cultured at 30°C for 4 to 6 days, 1 ml of the culture solution was transferred to 100 ml of the medium, and cultured again at 30°C. This transplant is 2
The bacteria were separated by dilution plate method in the third time (in this case, 15 g of agar was added to the medium).

(2) Serratia marsetuscens...glucose
0.5% (weight, same below), NH ₄ Cl0.1%, Mg
(NO ₃ ) ₂・6H ₂ O0.02%, CuCl ₂・2H ₂ O0.001%,
FeCl ₃ 6H ₂ O 0.001%, K ₂ HPO ₄ 0.1%,
Dispense 50 ml of a medium consisting of 0.05% KH ₂ PO ₄ and 0.1% methyl mercaptan into a 300 ml Erlenmeyer flask, and after autoclaving, add about 1 g of soil and culture at 30°C for 4 to 5 days. 1 ml of the culture solution was inoculated into the same volume of the same medium. This was repeated three times to collect bacteria.

A comparison of the ability of the two types of effective bacteria obtained as above to assimilate malodorous components revealed that Alcaligenes odorans was found to be more effective when 10 g of sodium acetate, 10 g of sodium propionate, and 10 g of sodium butyrate were added to the above medium. However, 73% of sodium acetate, 64% of sodium propionate, and 61% of sodium butyrate are utilized, and all other components are utilized.
100% assimilated, while Serratia marsetuscens had the ability to reduce 130 ppm methyl mercaptan to 15 ppm.

The deodorizer of the present invention contains the above-mentioned Alcaligenes odorans and/or Serratia marsetuscens as an active ingredient, and in a preferred embodiment, a medium containing these alone or in combination is treated with an adsorbent carrier such as zeolite, pearlite, or activated carbon. It is made into a solid deodorizer by adsorbing it on the water. The amount of effective bacteria adsorbed is preferably about 10 ⁶ to 10 ⁸ cells per gram of carrier.

(Action/Effect) The microbial deodorizer of the present invention obtained as described above effectively utilizes volatile fatty acids and mercaptan compounds, which are the main malodorous substances of various malodor sources, and has a deodorizing effect. has persistence, e.g.
When sprayed on pig farms or poultry farms, the active ingredients in the deodorizer multiply while assimilating the malodorous components, so it can provide excellent deodorizing effects over a long period of time. Therefore, unlike conventional physical and chemical deodorizers, it does not contaminate the surrounding area and exhibits excellent deodorizing effects over a long period of time, so it can be used not only as a general source of bad odors, but also in industrial environments such as pig farms and poultry farms. It is also useful for various purposes.

Next, the present invention will be specifically explained with reference to Examples. In the text, parts or percentages are based on weight unless otherwise specified.

Example 1 A culture solution (8.5×
10 ⁸ cells/g) was fixed on 100 parts of activated carbon while stirring with a mixer to obtain the microbial deodorizer of the present invention (1.5×10 ⁸ cells/g). Using this, harmful gases from human feces in urinary facilities can be removed with a liquid volume of 2.6 and a liquid depth of 1.380±5 [mmH].
(Cylindrical glass tube 50φ x 1500H), aeration amount 0.6 + 0.05
[/min], aeration intensity of 13.8 [m ³ /m ³ Hr], and 1000 ppm of the above deodorizing agent was added for the experiment. Raw odor gas concentration from human waste facility: ammonia 50ppm, lower fatty acids 10.2ppm, hydrogen sulfide 23.85ppm, methyl mercaptan 3.41ppm, dimethyl sulfide
6.07 ppm, odor concentration 31000 (odor bag method) was passed through a glass cylindrical tube under the above conditions while degassing. After 5 days, we measured the concentration of foul-smelling gas that was generated and found that it was less than 0.1 ppm of ammonia and hydrogen sulfide.
0.02ppm or less, lower fatty acids 0.1ppm or less, methyl mercaptan 0.17ppm, dimethyl sulfide
The odor concentration was 2.06 ppm and 880.

When a blank sample without deodorizing agent was tested under the same conditions for 5 days, the odor was strong and the odor concentration was 12,300. Ammonia was detected using a Gastetsu detector tube, and hydrogen sulfide, lower fatty acids, methyl mercaptan, and dimethyl sulfide were detected using a gas chromatograph (Shimadzu GC-7A model). Significant sensory effects were also obtained.

Example 2 Culture solution of Serratia marsetuscens screened and cultured from the soil (7.6×10 ⁸ cells/
g) Add 20 parts of perlite while stirring with a mixer.
The microbial deodorizer of the present invention was obtained by fixing 100 parts (1.3×10 ⁸ cells/g). Using this product, the same original odor gas concentration was treated under the same conditions as in Example 1, and the concentration of the treated odor gas was measured after 5 days.
0.02 ppm, methyl mercaptan 0.13 ppm, and dimethyl sulfide 1.03 ppm. Odor concentration is 900
It was hot. Significant sensory effects were also obtained.

Example 3 A culture solution of Serratia marcescens screened and cultured from the soil (7.6×10 ⁸ cells/
g) 10 parts of Alcaligenes odorans culture solution (8.5 x 10 ⁸ cells/g) screened and cultured from the pig manure compost was fixed on 100 parts of activated carbon while stirring with a mixer to produce the microorganism of the present invention. A deodorizer was obtained (1.1×10 ⁸ Cell/g). Using this,
The same original odor gas concentration was treated under the same conditions as in Example 1, and the concentration of the treated odor gas was measured after 5 days.
The concentrations were less than 0.1 ppm, hydrogen sulfide 0.01 ppm, methyl mercaptan 0.09 ppm, and dimethyl sulfide 0.03 ppm. The odor concentration was 730. Significant sensory effects were also obtained.

Example 4 A culture solution (7.6×
10 ⁸ cell/g) was fixed to 100 parts of pearlite in the same manner as in Example 1. A microbial deodorizer of the present invention was obtained by filling 5 g each of this with a polyvinyl alcohol resin film while heat-sealing with an automatic filling machine. Collect 20 to 30g of this into the toilet for the first time.
After one week, the odor intensity in the toilet decreased from 5 to 2 to 1 in the pre-injection sensory test.

Example 5 10 parts of the above-mentioned Alcaligenes odolans culture solution (8.5 x 10 ⁸ cells/g) and 10 parts of the above-mentioned Serratia marsetuscens culture solution (7.6 x 10 ⁸ cells/g) were mixed with 80 parts of water. Add 1 ton of chicken manure to the chicken manure dumping site.
When 2 kg of the mixed solution was sprayed and mixed, 5
After a few days, the fatty acid, sulfur, and ammonia odors characteristic of chicken manure were significantly improved.

Example 6 The present invention had the same effects as in Example 1 except that Alcaligenes odorans screened and cultured from sewage sludge was used instead of Alcaligenes odorans screened and cultured from the pig manure compost. A microbial deodorizer was obtained.

Example 7 Instead of the Serratia marsetuscens screened and cultured from the soil, Serratia marsetuscens screened and cultured from the sludge of a factory septic tank was used.
A microbial deodorizer of the present invention having the same effects as in Example 1 was obtained except for using marcetuscens.

Claims (1)

[Scope of Claims] 1. A microbial deodorizer comprising Alcaligenes odorans and/or Serratia marsetuscens as an active ingredient. 2. The microbial deodorizer according to claim 1, further comprising an adsorbent material such as zeolite, perlite, or activated carbon as a carrier.