JPH0211299B2 - - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION [Field of Application of the Invention] The present invention relates to a reaction method and apparatus using immobilized enzymes, and particularly to a reaction method and apparatus suitable for particulate immobilized enzymes.

[Prior art]

Packed columns, fluidized beds, stirring tanks, and the like have conventionally been used as reaction apparatuses using particulate immobilized enzymes. The characteristics and problems of each of these types are, firstly, packed columns have a large reaction area, but have a large pressure loss, are prone to clogging, and are difficult to remove gas and heat. There is a problem with this. Next, fluidized beds and stirred tanks are superior to packed columns in terms of problems such as pressure loss and clogging, as well as gas removal and heat removal.
There is a problem that the reaction area is inferior to a packed column.

[Purpose of the invention]

An object of the present invention is to provide an efficient immobilized enzyme reaction method and apparatus by solving the problems of the packed column, fluidized bed, etc.

[Summary of the invention]

In the present invention, a reaction solution is supplied to a reaction tank divided into multiple stages and filled with particulate immobilized enzyme with a space provided, a pulsation is applied to the reaction solution by a method of aseptically isolating the reaction solution from the outside, and the particles are By suspending the immobilized enzyme in each stage and taking out the reaction product, the contact efficiency between the immobilized enzyme particles and the reaction solution is improved, and
The interior of the reaction apparatus is divided into multiple stages of small chambers using perforated plates to prevent pressure build-up due to the weight of the immobilized enzyme, and
By providing a void in each chamber, the immobilized enzyme is suspended and the reaction is made uniform.

〔Example〕

An embodiment of the present invention will be described below with reference to FIG. In Fig. 1, 1 is the main body of the reaction device, the interior of which is divided into multiple stages of small chambers 20 to 24 by perforated plate 2, and 3 is particulate immobilized enzyme filled in each of the small chambers 20 to 24. In order to fluidize and suspend the enzyme particles, the container is filled with an appropriate amount of air at the top. 4 and 5 are reaction liquid inlet nozzles and reaction product outlet nozzles provided at the lower and upper parts of the main body 1; 6 is a pulse generator consisting of a diaphragm 7 and a reciprocating device 8 that operates it;
It is connected to the lower end of the main body 1 via a conduit 9.

Thus, the reaction liquid is supplied into the main body 1 from the inlet nozzle 4, and each small chamber 20 to 2 divided by the perforated plate 2
The immobilized enzyme 3 is sequentially passed through the packed bed of No. 4 to react, and is taken out from the outlet nozzle 5 as a reaction product.

In this case, by applying pulsations to the reaction liquid by the pulse generator 6, the immobilized enzyme 3 in each of the small chambers 20 to 24 is fluidized and suspended, and the pulsation is repeated, so that the reaction liquid in the main body 1 flows. becomes turbulent,
The contact surface of the immobilized enzyme 3 particles with the reaction solution is vigorously renewed, and the enzyme reaction efficiency is significantly improved.

In addition, since the packed bed of immobilized enzyme 3 is divided into multiple stages of small chambers 20 to 24 by perforated plate 2,
The flow of the filled particles in the main body 1 can be made uniform, and compaction can be prevented by freely adjusting the thickness of the packed layer of the filled particles in each chamber.
The reaction efficiency can be further improved.

Furthermore, by pulsating the immobilized enzyme 3, it becomes easy to remove air bubbles attached to the immobilized enzyme 3, and it is possible to prevent a decrease in efficiency due to channeling, etc., which is a problem in the case of a packed tower.

In this embodiment, a diaphragm is used as a device for applying pulsation, and this diaphragm isolates the outside and prevents deactivation of the enzyme or deterioration of performance due to biological contamination by external germs.

In the above example, a diaphragm and a reciprocating device were shown as the pulse generator, but it can be used in the same way as above as long as it gives pulsation to the reaction solution and can be isolated from the outside in a sterile manner. effect can be obtained.

FIG. 2 shows another embodiment of the present invention,
A heat exchanger 10 provided with a cooling water inlet nozzle 11 and an outlet nozzle 12 at both ends is disposed within the main body 1 to improve the ability to remove reaction heat.

〔Effect of the invention〕

As described above, the present invention supplies a reaction solution to a reaction tank divided into multiple stages and filled with particulate immobilized enzyme with a space provided, and the reaction solution is pulsated by a method of aseptically isolating the reaction solution from the outside. Since the particulate immobilized enzyme is suspended in each stage and the reaction product is taken out, the renewal efficiency of the contact surface between the immobilized enzyme particles and the reaction solution is improved, and the immobilization In addition to being able to improve the enzyme reaction efficiency, the inside of the reaction device is divided into multiple stages of small chambers using perforated plates, and each chamber is filled with particulate immobilized enzyme, leaving an empty space at the top. , preventing compaction of the immobilized enzyme particles in the reaction device and suspending the immobilized enzyme;
The flow can be made uniform, and the reaction efficiency can be further improved. Furthermore, since the pulse generation mechanism is attached via a diaphragm, it can be biologically and chemically isolated from the outside, thereby preventing enzyme deactivation and performance degradation due to biological and chemical contamination.

[Brief explanation of drawings]

FIG. 1 is a longitudinal sectional view showing one embodiment of the immobilized enzyme reaction device according to the present invention, and FIG. 2 is a longitudinal sectional view showing another embodiment. 1... Main body, 2... Perforated plate, 3... Immobilized enzyme,
4, 11... Inlet nozzle, 5, 12... Outlet nozzle, 6... Pulse generator, 7... Diaphragm, 8... Reciprocating device, 9... Conduit, 10... Heat exchanger, 20-24 ...Komuro.

Claims (1)

[Claims] 1. A reaction solution is supplied to a reaction tank divided into multiple stages and filled with particulate immobilized enzyme with a space provided, and pulsation is applied to the reaction solution by a method of aseptically isolating the reaction solution from the outside. ,
An immobilized enzyme reaction method characterized in that the particulate immobilized enzyme is suspended in each stage and the reaction product is taken out. 2. Divide the inside of the main body of the reaction apparatus, which has a reaction liquid inlet at the bottom and a reaction product outlet at the top, into multiple stages using perforated plates, and leave an empty space at the top of each stage to store the particulate immobilized enzyme. The immobilized enzyme is multi-layered by filling the main body with a pulse generator provided at the bottom of the main body to generate pulsations in the reaction solution inside the main body by means of aseptic isolation from the outside, and particulate immobilized enzyme is placed in each stage. An immobilized enzyme reaction device characterized by being configured to float. 3. The immobilized enzyme reaction device according to claim 2, wherein a heat exchanger is disposed within the main body.