JPH021534B2 - - Google Patents

Description

[Detailed description of the invention]

The present invention relates to an ion exchange resin composition that allows anions and/or cations in a liquid to be analyzed quickly and sensitively by high performance liquid chromatography. Conventionally, as an ion exchange resin composition used in a method of analyzing anions or cations in a solution by high performance liquid chromatography, The Dow Co., Ltd.
Chemical Company's products are well known. This is an ion exchange composition (Japanese Patent Application Laid-Open No. 77290/1983) consisting of two types of ion exchange resins, an anion exchange resin and a cation exchange resin with different particle sizes, one of which is an anion exchange resin and the other a cation exchange resin. However, in this known ion exchange resin composition, when anions are analyzed, the large particles must be composed of a cation exchange resin and the small particles must be composed of an anion exchange resin. On the other hand, in order to analyze cations using the composition obtained in this manner, the measurement requires an extremely long time and cannot be practically used. On the other hand, when analyzing cations, the large particles must be made of an anion exchange resin and the small particles must be made of a cation exchange resin, and the resin composition obtained by constructing in this way has anion exchange resins. Even if an attempt is made to analyze this, the measurement would take an extremely long time in this case, making it practically unusable. Furthermore, conventional ion exchange resin compositions other than the above-mentioned compositions have the disadvantage that it takes an extremely long time for ion elution. As a way to solve these drawbacks, attempts have been made to convert the separation column to a smaller, smaller-volume one, but with this conversion, the separation pressure increases, and because of this pressure increase, ion chromatography The glass separation column, the ion exchange resin packed in the column, the Teflon tube, etc. in the GRAPHIE apparatus were often damaged. Therefore, when conventional ion exchange resins are used, it is not possible to replace the separation column with a smaller one, and the analysis is forced to take a long time. Therefore, in view of the current situation, the present inventors have discovered that high-performance liquid chromatography can quickly analyze anions and/or cations in a liquid, and can also be used in combinations depending on the type of ions. In order to obtain an ion exchange resin composition that does not require changing the particle size of the resin, we have conducted various studies and found that a microparticle insoluble synthetic resin matrix with hydrophilic groups on the effective surface and no ion exchange groups is essential. A composition in which this substrate is further mixed with an anion exchange resin and/or a cation exchange resin having approximately the same particle size as the substrate is used depending on the type of ion to be analyzed. It was discovered that anions and/or cations in a liquid can be analyzed quickly and with high sensitivity without changing the particle size of the liquid, and based on this discovery, the present invention was completed. That is, the present invention is made by mixing a fine particle insoluble synthetic resin matrix having an ion exchange group on its effective surface with a fine particle insoluble synthetic resin matrix having a hydrophilic group on its effective surface and no ion exchange group. It is an ion exchange resin composition. The present invention will be explained in detail below. The particulate insoluble synthetic resin matrix having ion exchange groups on its effective surface used in the present invention includes anion exchange resins and cation exchange resins. Examples of the anion exchange resin include known anion exchange resins having a particle size of 2 to 50 microns, preferably 5 to 25 microns, such as "strongly basic anion exchange resin MCI GEL" manufactured by Mitsubishi Chemical Industries, Ltd.
CA04S (base resin: styrene divinylbenzene copolymer, type of ion exchange group: −N ⁺ (CH ₃ ) ₃ , ion exchange capacity: 1.1 meq/ml, particle size: 11 μm, substituted form when used: OH ^- type , degree of crosslinking; 4%)", "same
MCI GEL CA06S (base resin: styrene divinylbenzene copolymer, type of ion exchange group: −N ⁺
(CH ₃ ) ₃ , ion exchange capacity; 1.2 meq/ml, particle size;
11μm, substituted form when used; OH ^-type , degree of crosslinking;
6%)", the company's "high porous strong basic anion exchange resin MCI GEL CDR-10 (base resin: styrene divinylbenzene copolymer, type of ion exchange group: -N ⁺ (CH ₃ ) ₃ , ion exchange Capacity: 0.3meq/
ml, particle size: 7 to 8 μm, substitution form when used: OH ^- type), Hitachi Gel #3011-N, manufactured by Hitachi, Ltd.
(Base resin: styrene divinylbenzene copolymer,
Type of ion exchange group: −N ⁺ (CH ₃ ) ₃ , ion exchange capacity: approximately 0.7 meq/ml, particle size: 10 μm, substituted form during use: OH ^- type), Toyo Soda Kogyo Co., Ltd. “Strong anion Exchange gel IEX-220SA (base resin: styrene divinylbenzene copolymer, type of ion exchange group: -N ⁺ (CH ₃ ) ₃ , ion exchange capacity: 3.7meq/ml
Particle size: 10 μm, substitution form when used: OH ^- type), silica-based strong anion exchange gel IEX manufactured by the company
-260SA SIL (base resin: porous silica, type of ion exchange group: -N ⁺ (CH ₃ ) ₂ CH ₂ C ₆ H ₅ , ion exchange capacity: 0.5 meq/g or more, particle size: 10 μm, replacement during use OH ^- type)” and Bio-Rad’s ion exchange resin Aminex A-29 (base resin: styrene divinylbenzene copolymer, type of ion exchange group: −N ⁺ (CH ₃ ) ₃ , ion exchange capacity;
1.4 meq/ml, particle size: 5 to 8 μm, substituted form when used: OH ^- type, degree of crosslinking: 8%). Next, as a cation exchange resin, the particle size is 2 to 50.
Known cation exchange resins having a particle size of 5 to 25 microns, preferably 5 to 25 microns, can be mentioned, for example, “Strongly acidic cation exchange resin MCI GEL” manufactured by Mitsubishi Chemical
CK08S (base resin: styrene divinylbenzene copolymer, type of ion exchange group: -SO ₃ ^- , ion exchange capacity: 1.5 meq/ml or more, particle size: 11 μm, substitution type when used: H ⁺ type, degree of crosslinking ; 8%)", Hitachi Gel #3011-S (base resin: styrene divinylbenzene copolymer, type of ion exchange group: -SO ₃ ^- , ion exchange capacity: approximately 4.0meq/
ml, particle size: 10μm, substitution form when used: H ⁺ type), Toyo Soda Kogyo Co., Ltd., "Strong cation exchange gel"
IEX-210SC (base resin; styrene divinylbenzene copolymer, type of ion exchange group; -SO ₃ ^- ,
Ion exchange capacity: 4.2meq/g or more, particle size 10μm,
Substituted form when used: H ⁺ type) and Bio-Rad's ion exchange resin Aminex A-8 (base resin: styrene divinylbenzene copolymer, type of ion exchange group: -SO ₃ ^- , ion exchange capacity;
2.0 meq/ml, particle size: 5 to 8 μm, substituted form when used: H ⁺ type, degree of crosslinking: 10%). In addition, fine particle insoluble synthetic resin substrates having hydrophilic groups and no ion exchange groups on their effective surfaces include:
on the effective surface of the polymer gel with a particle size of 2 to 50 microns, preferably 5 to 25 microns, such as OH groups.
Hydrophilic groups that are not ion exchange groups are introduced, such as Hitachi Gel #3011-O (base resin: styrene divinylbenzene copolymer, type of hydrophilic group: -CH ₂ OH, amount introduced: approximately 0.8 mmol/
ml, particle size: 11 to 13 μm) and Separation Resin TAQP-2 (base resin: acrylic resin, type of hydrophilic group: -OH, particle size: 10 μm) manufactured by Mitsubishi Chemical Industries, Ltd.
m)", and "TAQP-3 (base resin; acrylic resin, type of hydrophilic group: -OH, particle size: 10μ
m)" etc. The selection of anion exchange resin and/or cation exchange resin to be mixed with the above-mentioned insoluble synthetic resin that has hydrophilic groups on the effective surface and does not have ion exchange groups (hereinafter abbreviated as hydrophilic separation resin) is determined by analysis. It varies depending on the type of ion. Namely, fluorine ion (hereinafter abbreviated as F ^- ), chloride ion (Cl ^- ), phosphate ion (PO ^3- ₄ ), nitrate ion (NO ₃ ^- ), sulfate ion (SO ² ₄ ^- ), and thiosulfate ion. When attempting to analyze anions such as (S ₂ O ^2- ₃ ), an anion exchange resin is selected and used. On the contrary, sodium ions (Na ⁺ ), ammonium ions (NH ₄ ⁺ ), magnesium ions (Mg ²⁺ ), and calcium ions (Ca ²⁺ ), etc.
When attempting to analyze cations, select and use a cation exchange resin. With columns that use both cation exchange resins and anion exchange resins, anions or cations can be analyzed using the same column by changing the eluent. The mixing ratio of the anion exchange resin and/or cation exchange resin and the hydrophilic separation resin is preferably determined depending on the type of ion to be analyzed or the elution time (retention time).
Usually 1:0.1-200 in weight ratio, preferably 1:1
~120. In other words, the ratio of the latter to the former is
If the number is more than 200, the eluent will diffuse more, resulting in poor separation and lower accuracy. On the other hand, when it is less than 0.1, it has the disadvantage that the elution time becomes longer.
In order to overcome this drawback, it is necessary to change the separation column to a smaller one with a smaller volume and to increase the separation pressure, which is an inconvenience. Maintaining a high separation pressure carries the risk of damaging the separation column (made of glass), packing material, and Teflon tube. Preferred specific examples of the mixing ratio of anion exchange resin and/or cation exchange resin and hydrophilic separation resin include anions such as F ^- , PO ^3- ₄ , Cl ^- , SO ^2- ₄ In the case of 1:1 to 20 is particularly preferable,
In the case of an ion with _a very long elution time such as S ₂ O ^2-3 , a ratio of 1:80 to 120 is particularly preferable. Na ⁺ , K ⁺ ,
For cations such as NH ₄ ⁺ , Mg ²⁺ and Ca ²⁺ 1:1 to 10 is particularly preferred. When anions or cations are measured using the same column using different eluents, it is particularly preferable to mix each resin at a ratio of 1:1 to 5. The mixing method may be any conventional method for mixing an anion exchange resin and a hydrophilic separation resin, or for mixing a cation exchange resin and a hydrophilic separation resin.
For example, it is preferable to weigh each component, put it in a beaker, add water or an eluent, stir it with a glass rod, and further mix it uniformly with an ultrasonic cleaner. When mixing an anion exchange resin, a cation exchange resin, and a hydrophilic separation resin, first mix the anion exchange resin and the hydrophilic separation resin in the manner described above, and then mix the cation exchange resin and the hydrophilic separation resin. and a hydrophilic separation resin in the same manner, and finally, both mixtures are mixed using a dilute alkaline solution or eluent, stirred with a glass rod, and then mixed uniformly with an ultrasonic cleaner. In this way, the ion exchange resin composition of the present invention is obtained. In order to analyze anions and/or cations in a solution by high performance liquid chromatography using the ion exchange resin composition of the present invention, a commercially available liquid chromatography part as shown in FIG. An example is a device that combines a meter. That is, an eluent storage tank 1, a liquid feeding pump 2, a pressure gauge 3, a sampler 4, a separation column 5, a buffer removal column 6, and a fluid measurement flow cell 7 are connected in this order through a tube, and the flow cell is connected to an electrical conductor. An example is a device in which a meter 8 and a recorder 9 are associated. The buffer removal column 6 is a column that removes ions in the eluent having a charge opposite to that of the cations or anions to be analyzed. By using this buffer removal column 6, sample ions can be directly detected with extremely low background. Next, Table 1 shows specific examples of high performance liquid chromatography components that constitute this apparatus.

[Table] The ion exchange resin composition obtained according to the present invention contains anions such as F ^- , Cl ^- , PO ^3- ₄ , NO ^- ₃ , SO ^2- ₄ and S ₂ O ^2- ₃ and/or Na ⁺ , K ⁺ , NH ₄ ⁺ ,
Cations such as Mg ²⁺ and Ca ²⁺ can be separated very sensitively and rapidly. Furthermore, the ion exchange resin composition of the present invention has a feature that there is no need to change the particle size of the combined resin each time depending on the type of ion to be analyzed.
Furthermore, by using the ion exchange resin composition of the present invention, the pressure of the eluent pump may be lowered or
Alternatively, the separation column can be changed to a smaller, smaller capacity separation column. Hereinafter, the present invention will be explained in more detail by showing examples and application examples. Example 1 60 mg of anion exchange resin (MCI GEL CDR-10, manufactured by Mitsubishi Chemical Industries, Ltd.) and a fine particle insoluble synthetic resin substrate (Hitachi Gel #3011-) that has hydrophilic groups on its effective surface and no ion exchange groups. 0, manufactured by Hitachi, Ltd.), put them in a beaker, add water, stir with a glass rod, and mix uniformly with an ultrasonic cleaner to prepare the ion exchange resin composition of the present invention. Obtained. Example 2 60 mg of anion exchange resin (MCI GEL CA04S, manufactured by Mitsubishi Chemical Industries, Ltd.) and a fine particle insoluble synthetic resin substrate (Hitachi Gel #3011-0, which has hydrophilic groups on the effective surface and no ion exchange groups) Manufactured by Hitachi, Ltd.)
600 mg of each was weighed out, placed in a beaker, water was added, stirred with a glass rod, and mixed uniformly using an ultrasonic washer to obtain the ion exchange resin composition of the present invention. Example 3 150 mg of a cation exchange resin (MCI GEL CK08S, manufactured by Mitsubishi Chemical Industries, Ltd.) and a fine particle insoluble synthetic resin substrate (Hitachi Gel #3011-0, which has a hydrophilic group on its effective surface and no ion exchange group) (manufactured by Hitachi, Ltd.) were weighed out, placed in a beaker, added with water, stirred with a glass rod, and mixed uniformly in an ultrasonic cleaner to obtain the ion exchange resin composition of the present invention. . Example 4 150 mg of anion exchange resin (MCI GEL CA04G, manufactured by Mitsubishi Chemical Industries, Ltd.) and a fine particle insoluble synthetic resin (Hitachi GEL #3011-0, Hitachi GEL #3011-0, which has hydrophilic groups on the effective surface and no ion exchange groups) Manufactured by Seisakushosha)
750 mg of each was weighed out, placed in a beaker, water was added, stirred with a glass rod, and mixed uniformly with an ultrasonic cleaner. In addition, cation exchange resin (MCI GEL CK08S,
Weighed 150 mg (manufactured by Mitsubishi Chemical Industries, Ltd.) and 750 mg of a fine particle insoluble synthetic resin (Hitachi Gel #3011-0, manufactured by Hitachi, Ltd.) that has hydrophilic groups on its effective surface and does not have ion exchange groups, and placed them in a beaker. After adding water, the mixture was stirred with a glass rod and mixed uniformly using an ultrasonic cleaner. Next, both mixtures were placed in a beaker, a small amount of 0.1N sodium hydroxide was added thereto, and after stirring with a glass rod, the mixture was uniformly mixed using an ultrasonic washer to obtain the ion exchange resin composition of the present invention. Application Example 1 In the high performance liquid chromatography apparatus shown in FIG. 1, each part was installed as described in Experimental apparatus 1 in Table 1, and the ion exchange resin composition prepared in Example 1 was placed in the separation column 5. Buffer removal column 6 contains a cation exchange resin (Amberlite).
CG-120Type (manufactured by Rohm and Haas) were each filled using a slurry method. Eluent (solution containing 0.003M _NaHCO3 and _{0.0024MNa2CO3 )}
was stored in the eluent storage tank 1, and the liquid was fed by the liquid feeding pump 2 at a flow rate of 1.88 ml/min. The room temperature is 17-23℃,
The conductivity was measured at 10μ and the recorder at 1V. Next, F ^- , Cl ^- , PO ^3- ₄ and
A sample containing SO ^2-4 was prepared, 30μ of this was sampled with sampler 4, and injected into separation column 5. A chromatogram in which each ion was separated was obtained, and as a result, the result shown in FIG _. 3 was obtained. F ^- : 2.17μg/30μ Cl ^- : 5.07〃 PO ^3- ₄ : 12.95〃 SO ^2- ₄ : 16.38〃 From these results, the composition of the present invention has F ^- , Cl ^- ,
It can be seen that anions such as PO ^3- ₄ and SO ^2- ₄ can be well separated and detected. Next, a calibration curve was created to determine the sensitivity of this device to fluorine ions (F ^- ). Special grade NaF was used as a standard material. 2.5 mg of NaF dried in an air dryer was dissolved in distilled water to make 50 ml and used as a standard solution. Collect a certain amount of this standard solution with a microsyringe, inject it into the separation column 5, record the output with a recorder, and calculate the peak height (cm) and injection amount (μg).
A calibration curve was obtained from The calibration curve for this fluorine ion was as shown in FIG. 2, and a straight line passing through the origin was obtained. This result shows that the ion exchange resin composition of the present invention can quantitatively and sensitively analyze fluorine ions in a solution. In addition, in order to determine the sensitivity to Cl ^- , PO ^3- ₄ , NO ₃ ^- and SO ^2- ₄ , NaCl 58.6 mg/50 ml was used as a standard solution in the same manner as in the case of F ^- above.
(distilled water), NaH ₂ PO ₄・2H ₂ O 156.0mg/50ml
(〃), NaNO ₃ 84.9mg/50ml (〃) and
Prepare 142.0 mg/50 ml (〃) of Na ₂ SO ₄ , collect a certain amount of this standard solution with a microsyringe, inject into separation column 5, record the output with a recorder, and calculate the peak height (cm) and injection. A calibration curve was created from the amount (μg). As a result, as in the case of F ^- , Cl ^- ,
The calibration curves for PO ^3- ₄ , NO ₃ ^- and SO ^2- ₄ were also straight lines passing through the origin, indicating that the ion exchange resin composition of the present invention can quantitatively and sensitively analyze various anions in a solution. I understand. Application Example 2 In the high performance liquid chromatography apparatus shown in FIG. 1, each part was installed as described in Experimental Apparatus 2 in Table 1, and the ion exchange resin composition prepared in Example 2 was placed in the separation column 5. Buffer removal column 6 contains a cation exchange resin (Amberlite).
CG-120Type (manufactured by Rohm and Haas) were each filled using a slurry method. Eluent (solution containing 0.003MNaHCO3 and 0.0024MNa ₂ CO ₃ )
was stored in the eluent storage tank 1, and the liquid was fed by the liquid feeding pump 2 at a flow rate of 1.92 ml/min. The room temperature is 17-23℃,
The electrical conductivity was measured at 15 μ and a Kiyoku meter at 5 mV. Next, fluorine ions (F ^- ), chloride ions (Cl ^- ), phosphate ions (PO ^3- ₄ ), nitrate ions (NO ₃ ^- ), and sulfate ions (SO ^2- ₄ ) are added as follows. A sample was prepared, 40μ of it was collected using a sampler 4, and the sample was injected into a separation column 5. A chromatogram in which each ion was separated was obtained, and as a result, the result shown in FIG. 4 was obtained. Note F ^- : 1.56μg/40μ Cl ^- : 3.64〃 PO ^3- ₄ : 17.4〃 NO ₃ ^- : 12.7〃 SO ^2- ₄ : 17.6 〃 Application example 3 In the high performance liquid chromatography apparatus shown in Figure 1, each part were prepared as described in Experimental apparatus 4 in Table 1, the separation column 5 contained the ion exchange resin composition prepared in Example 3, and the buffer removal column 6 contained the anion exchange resin (Amberlite).
CG-400Type (manufactured by Rohm and Haas) were each filled using a slurry method. Then, the eluent (0.005MHNO ₃ solution) was stored in the eluent storage tank 1, and the liquid was fed by the liquid feeding pump 2 at a flow rate of 2.49 ml/min. Set the room temperature to 17-23℃ and measure the electrical conductivity meter at 10μ.
, and the recorder was measured at 1V. Next, sodium ions (Na ⁺ ),
A sample containing potassium ions (K ⁺ ) and ammonium ions (NH ₄ ⁺ ) is prepared, 50μ of this is collected with a sampler and injected into a separation column.
When a chromatogram in which each ion was separated was obtained, the results shown in FIG. 5 were obtained. Na ⁺ : 9.2 μg/50 μ K ⁺ : 23.4〃 NH ₄ ⁺ : 9.0〃 From these results, it can be seen that the composition of the present invention can well separate and detect cations such as Na ⁺ , K ⁺ and NH ₄ ⁺ . . Next, a calibration curve was created to determine the sensitivity of this device to Na ⁺ , K ⁺ and NH ₄ ⁺ . Na ₂ SO ₄ 142.04 mg/50 ml (distilled water) as standard solutions, respectively.
Prepare K ₂ SO ₄ 174.27 mg/50 ml (〃) and (NH ₄ ) ₂ SO ₄ 132.14 mg/50 ml (〃), collect a certain amount of this standard solution with a microsyringe,
Injected into a separation column, recorded the output with a recorder, and created a calibration curve from the peak height and injection volume.
A straight line passing through the origin was obtained. From these results, the composition of the present invention shows that
It can be seen that Na ⁺ , K ⁺ and NH ₄ ⁺ can be quantitatively analyzed with good sensitivity. Application Example 4 In the high performance liquid chromatography apparatus shown in Figure 1, each part was installed as described in Experimental Apparatus 4 in Table 1, and the ion exchange resin composition prepared in Example 3 was removed from the separation column. The column contains anion exchange resin (Amberlite CG-400TyPe,
(manufactured by Rohm and Haas) by a slurry method. and eluent [0.005MHNO ₃ ,
0.001M para-phenylenediamine sulfate (P-
phenylene-diamine sulphate)] was stored in the elution storage tank 1, and the liquid was fed by the liquid feeding pump 2 at a flow rate of 1.54 ml/min. Set the room temperature to 17 to 23℃ and measure the electrical conductivity.
10μ, and the recorder was measured at 1V. Next, a sample containing Mg ²⁺ and Ca ²⁺ was prepared as described below, 50μ of this was sampled with a sampler, and injected into separation column 5, and a chromatogram in which each ion was separated was obtained. The results shown in the figure were obtained. Note: Mg ²⁺ : 9.7 μg/50 μ Ca ²⁺ : 24.0 Next, a calibration curve was created to determine the sensitivity of this device to Ca ²⁺ and Mg ²⁺ . CaCl ₂ 110.0 mg/50 ml (distilled water) and
120.3 mg/50 ml of MgSO ₄ was prepared, a certain amount of this standard solution was collected with a microsyringe, injected into a separation column, the output was recorded with a recorder, and a calibration curve was created from the peak height and injection volume. , a straight line passing through the origin was obtained. From these results, the composition of the present invention has a
It can be seen that Ca ²⁺ and Mg ²⁺ can be quantitatively analyzed with good sensitivity. Application Example 5 In the high performance liquid chromatography apparatus shown in FIG. Removal column 6 contains cation exchange resin (Amberlite CG
-120Type, manufactured by Rohm and Haas) were each filled using the slurry method. The eluent (a solution containing 0.003 MNaHCO ₃ and 0.0024 MNa ₂ CO ₃ ) was stored in the eluent storage tank 1, and the liquid was fed by the liquid feed pump 2 at a flow rate of 2.52 ml/min. The room temperature is 17-23℃, the electrical conductivity is 15μ, and the meter is 5mV.
It was measured with Then, in the following manner, F ^- , Cl ^- , PO ^3- ₄ , NO ₃ ^-
Prepare a sample containing SO ^2- ₄ , collect 50μ with a sampler and inject it into a separation column,
When chromatograms were obtained in which each of the components was separated, the results shown in FIG. 7 were obtained. Note F ^- : 1.95μg/50μ Cl ^- : 4.54〃 PO ^3- ₄ : 21.8〃 NO ₃ ^- : 15.9〃 SO ^2- ₄ : 22.0〃 Next, in this application example 5, an anion exchange resin ( Amber light CG-
400Type, manufactured by Rohm and Haas), the eluent was a solution containing 0.005MHNO ₃ and 0.001M para-phenylenediamine sulfate, the flow rate of the liquid pump was 2.61 ml/min, and an electrical conductivity meter was used. The test was carried out under the same equipment conditions as Application Example 5 (the resin packed in the separation column was not changed), except that the electrical conductivity was set to 10 μ using CD-A manufactured by Chromatec, and the recorder was used for measurement at 1 V. Then, prepare a sample containing Na ⁺ , K ⁺ and NH ₄ ⁺ as described below, and add 50μ of this using a sampler.
When the sample was collected and injected into a separation column, and a chromatogram in which each ion was separated was obtained, the results shown in FIG. 8 were obtained. Na ⁺ : 9.2 μg/50 μ K ⁺ : 23.4〃 NH ₄ ⁺ : 9.0〃 From the above two test results, it can be concluded that the ion exchange resin composition of the present invention can be used for the analysis of not only anions but also cations. I understand.

[Brief explanation of drawings]

Figure 1 is a schematic illustration of a high-performance liquid chromatography apparatus used in an application example of the present invention, Figure 2 is a diagram showing a calibration curve for fluorine ion (F ^- ), and Figure 3 is a diagram showing a calibration curve for fluorine ions (F - ).
Figures 8 to 8 are diagrams showing ion chromatograms showing separation patterns of various ions. 1...Eluent storage tank, 2...Liquid pump, 3...
... Pressure gauge, 4 ... Sampler, 5 ... Separation column, 6 ... Buffer removal column, 7 ... Flow cell, 8 ... Electrical conductivity meter, 9 ... Recorder. stomach…
...F ^- , B...Cl ^- , H...PO ₄ ^3- , Ni...NO ₃ ^- ,
Ho...SO ₄ ^2- , He...Na ⁺ , To...NH ₄ ⁺ , Chi...
…K ⁺ , Li…Mg ²⁺ , Nu…Ca ²⁺ .

Claims (1)

[Scope of Claims] 1. A fine particle insoluble synthetic resin matrix having an ion exchange group on its effective surface and a fine particle insoluble synthetic resin matrix having a hydrophilic group on its effective surface and no ion exchange group are mixed. An ion exchange resin composition. 2. The composition according to claim 1, wherein the fine particle insoluble synthetic resin matrix having ion exchange groups on its effective surface is at least one selected from anion exchange resins and cation exchange resins. 3 The mixing ratio of the fine particle insoluble synthetic resin substrate having an ion exchange group on the effective surface and the fine particle insoluble synthetic resin substrate having a hydrophilic group on the effective surface and no ion exchange group is 1:0.1 to 200. A composition according to claim 1.