JPH0225151B2 - - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to an apparatus for sequentially testing various properties of blood.

Conventionally, testing of blood samples involves setting up multiple test tubes containing each sample on a stand, etc., and measuring the number of red blood cells or white blood cells for each sample while transporting the stand. In some cases, a multi-item test was performed, first measuring the platelet count and thirdly testing the classification of white blood cells. In this case, when performing each test, the specimens were mixed up or the stands were mixed up, resulting in a discrepancy between the specimen number and the test data. Also,
Even if some of the test data regarding red blood cells is interrelated, it can be discovered later, but since the measurement of white blood cell count and the measurement of platelet count are independent measurements, it is important to It was impossible to discover the mistake afterwards. In addition, the white blood cell count is measured after hemolyzing the red blood cells, but if the hemolysis is insufficient, the red blood cells will be added, resulting in multiple measurement results.Furthermore, when measuring the platelet count, some types of specimen When an anticoagulant is added as a pretreatment for measurement, it causes aggregation, resulting in low measurement results even if the platelet count is normal.
It was difficult to discover these measurement errors later. The above-mentioned test errors have been increasing with the increase in the number of specimens due to the spread of blood tests and the automation of measuring devices, and have had a significant impact on patient diagnosis.

SUMMARY OF THE INVENTION An object of the present invention is to provide a blood test device that can determine whether white blood cells and red blood cells have been measured correctly.

The present invention will be explained below based on one embodiment shown in the drawings. Reference numeral 1 denotes a blood cell analyzer, which includes a sample processing section 3, a red blood cell detection section 5, a colorimetric measurement section 7, a white blood cell detection section 9, and a detection signal processing section 11. The sample processing unit 3 is
A predetermined sample is diluted or a hemolytic agent is added, and the red blood cell detection unit 5 and colorimetric measurement unit 7
and distributed to the white blood cell detection section 9. Red blood cell detection section 5, colorimetric measurement section 7, and white blood cell detection section 9
measures the distributed samples and generates a detection signal of 1 for each sample.
3, 15, and 17. The detection signal processing unit 11 calculates the number of red blood cells, hematocrit value, hemoglobin value, MCV, MCH, MCHC, and white blood cell count based on the detection signals 13, 15, and 17, and outputs them as information paired with the sample number. After performing such measurements on each sample contained in the test tube rack, each sample is sent to the platelet counter 19. Note that although this blood cell analyzer 1 is an automatic device, it does not necessarily need to be an automatic device.

The platelet counter 19 counts the number of platelets from a specimen from which red blood cells and white blood cells have been removed, and outputs the information in pairs with the specimen number.
This measurement is also performed on each sample contained in the test tube rack, and then each sample is transferred to the white blood cell sorting device 21.
sent to.

The leukocyte classification device 21 has a monitor 22 for visually observing a smear sample of a specimen that has been smeared onto a slide glass and subjected to treatments such as staining.
Classification of white blood cells is performed while visually observing the monitor 22.
This is done by pressing a classification button (not shown) provided on the classification counter 24 corresponding to the component of white blood cells or a classification button 33 similarly provided on the input section 23. This classification is also performed for each specimen contained in the test tube rack. When the classification is performed by the classification counter 24, the classification value is sent to the input unit 23 as information paired with the specimen number.

As shown in FIG. 2, the input section 23 includes a data transfer button 25, a platelet abundance determination button 27 consisting of three buttons: "high", "medium", and "low", a platelet aggregation determination button 29,
It consists of a white blood cell count determination button 31 consisting of three buttons "high", "medium", and "low", a white blood cell classification button 33, a specimen number input button 35, and a data processing section 37. The data processing section 37 stores signals obtained by pressing other buttons. Quantity judgment button 27, 3
1. Platelet aggregation determination button 29, white blood cell classification button 33
is pressed based on the visual result on the monitor 22. In other words, when visually observing a sample on a monitor, if it is determined that the platelet count is high,
Press the "high" button of the platelet count determination button 27. Then, a signal indicating that 400,000 platelets/cm ³ or more are present is stored in the data processing unit 37. Similarly, if you press the "low" button based on the visual result, 100,000 pieces/cm ³
A signal indicating that the following platelets are present is stored. When the "medium" button is pressed, a signal indicating the presence of approximately 100,000 ^{to 400,000 platelets/cm 3 is} stored. The platelet aggregation determination button 29 is pressed when platelets are visually observed to aggregate, and a platelet aggregation signal is stored in the data processing unit 37.

The white blood cell count determination button 31 is similarly pressed based on the visual result, and when the "high" button is pressed, a signal indicating that the white blood cell count is 12,000 cells/cm ³ or more is stored in the data processing unit 37, and " When the “low” button is pressed,
A signal indicating that the white blood cell count is less than 3000 cells/cm ³ is stored. When the "medium" button is pressed, a signal indicating that the white blood cell count is about 3000 cells/cm ³ to about 12000 cells/cm ³ is stored. The white blood cell classification button 33 is pressed to classify white blood cells when the white blood cells are not classified by the white blood cell classification counter 22. The sample number input button 35 is
This is for inputting the number of the specimen for which the above-described determination has been made based on the visual inspection results.
The data transfer button 25 is used to transfer information stored in the data processing section 37 to the arithmetic processing section 39.

Note that even if the data transfer button 25 is pressed before the quantity determination buttons 27 and 31 are pressed, various signals are not transferred.

The arithmetic processing unit 39 includes a memory 43, a platelet count determination device 45, and a white blood cell count determination device 47. The memory 43 receives information regarding red blood cells and white blood cells from the detection signal processing unit 11, the platelet count from the platelet counter 19, and white blood cell classification information from the input unit 23, and manages average values and calculates standard deviations for each input value. Processing such as statistical accuracy control is performed. Furthermore, the platelet aggregation signal is stored in the memory 43 at the input section 2.
3 is memorized. The platelet count determining device 45 receives the platelet count input from the memory 43 from the input unit 23.
Determine whether the platelet count matches the approximate value of the platelet count obtained from the same sample number. White blood cell count determination device 47
determines whether the white blood cell count input from the memory 43 matches the approximate value of the white blood cell count for the same specimen number obtained from the input section 23.

The printing device 41 outputs information from the memory 43 that has undergone statistical accuracy control and the platelet count determination device 45.
and the determination results of the white blood cell count determining device 47 are printed at the same time. In addition, since platelets have aggregation, memory 4
If the platelet aggregation signal is stored in No. 3, the determination result of the platelet count determining device 45 will be inconsistent. In this case, only the cohesion is printed, or both the cohesion and the mismatch are printed.

In this device, the white blood cell count obtained from the detection signal processing unit 11 is compared with the rough visual white blood cell count of the same specimen number obtained from the input unit 23 to determine whether they match. Therefore, if there is a discrepancy, it can be quickly determined that the hemolysis of the red blood cells is insufficient or that the sample has been mixed up in the sample processing section 3 or the leukocyte classification device 21, and measurement errors can be prevented. Further, the platelet count obtained from the platelet counter 19 is compared with the approximate platelet count obtained by visual observation of the same sample number obtained from the input section 23 to determine whether they match. Therefore, in the case of a mismatch, it can be quickly determined that the sample in the leukocyte classifier 21 and the sample in the platelet counter 19 are mistaken, and measurement errors can be prevented. Further, even if platelets are aggregated, it can be determined that the aggregation has occurred based on the visual result obtained from the input unit 23, so it is possible to prevent normal platelets from being judged as abnormal.

[Brief explanation of drawings]

FIG. 1 is a block diagram of a blood test apparatus embodying the present invention, and FIG. 2 is a block diagram of the input section of this apparatus. {9...white blood cell detection unit, 11...detection signal processing unit, 19...platelet count counter} counting device, 21...
...White blood cell classification device (visual inspection device), 23...Input unit, 41...Printing device, 43...Memory (storage device), {45...Platelet count determination device, 47...White blood cell count determination device} Comparator .

Claims (1)

[Claims] 1. A counting device that counts the number of at least one of white blood cells and platelets for the blood samples transferred in sequence, a storage device that stores the counted value, and another test that involves visual inspection at a location different from the counting device. It has a visual inspection device for performing a visual inspection on a specimen that is considered to be the same as the above-mentioned specimen, and a key for inputting the approximate number of at least one of the above-mentioned white blood cells and platelets incidentally found by the visual inspection in this visual inspection device. an input device that converts the approximate number into a signal together with information on the test results in the counting device; and a comparator that compares the signal representing the approximate number from the input device with the counted value stored in the storage device. and a device for printing information on the test results of the visual inspection device, the counted value, and the comparison result of the comparator.