JPH0225460B2 - - Google Patents
Info
- Publication number
- JPH0225460B2 JPH0225460B2 JP57140527A JP14052782A JPH0225460B2 JP H0225460 B2 JPH0225460 B2 JP H0225460B2 JP 57140527 A JP57140527 A JP 57140527A JP 14052782 A JP14052782 A JP 14052782A JP H0225460 B2 JPH0225460 B2 JP H0225460B2
- Authority
- JP
- Japan
- Prior art keywords
- gas
- electrode
- diaphragm
- tube
- permeable membrane
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000012528 membrane Substances 0.000 claims description 20
- 239000003779 heat-resistant material Substances 0.000 claims 1
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 20
- 229910002092 carbon dioxide Inorganic materials 0.000 description 10
- 239000001569 carbon dioxide Substances 0.000 description 10
- 239000011521 glass Substances 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 238000000855 fermentation Methods 0.000 description 4
- 230000004151 fermentation Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- WABPQHHGFIMREM-UHFFFAOYSA-N lead(0) Chemical compound [Pb] WABPQHHGFIMREM-UHFFFAOYSA-N 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000004745 nonwoven fabric Substances 0.000 description 2
- -1 polytetrafluoroethylene Polymers 0.000 description 2
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 2
- 239000004810 polytetrafluoroethylene Substances 0.000 description 2
- 229920002379 silicone rubber Polymers 0.000 description 2
- 239000004945 silicone rubber Substances 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 238000010025 steaming Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- IDCBOTIENDVCBQ-UHFFFAOYSA-N TEPP Chemical compound CCOP(=O)(OCC)OP(=O)(OCC)OCC IDCBOTIENDVCBQ-UHFFFAOYSA-N 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000010030 laminating Methods 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 229920002994 synthetic fiber Polymers 0.000 description 1
- 239000012209 synthetic fiber Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/403—Cells and electrode assemblies
- G01N27/404—Cells with anode, cathode and cell electrolyte on the same side of a permeable membrane which separates them from the sample fluid, e.g. Clark-type oxygen sensors
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Sampling And Sample Adjustment (AREA)
Description
【発明の詳細な説明】
本発明は、発酵工業等において、発酵槽内、お
よびガス排気管路内等のガス濃度の測定に使用さ
れる隔膜式ガス電極の支持装置に係る。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a support device for a diaphragm-type gas electrode used in the fermentation industry and the like to measure gas concentrations in fermenters, gas exhaust pipes, and the like.
一般に発酵工業において、培養中の発酵槽内の
培養液中に溶存する炭酸ガスと、排気ガス中の炭
酸ガスの濃度を測定することは、培養時における
状態の観察上、重要である。 Generally, in the fermentation industry, it is important to measure the concentration of carbon dioxide gas dissolved in the culture solution in the fermenter during cultivation and the concentration of carbon dioxide gas in the exhaust gas in order to observe the state during cultivation.
従来、培養中の発酵槽、排気ガスの炭酸ガス濃
度の測定には、非分散赤外線式炭酸ガス濃度計が
多く使用されてきたが、装置が高価であること、
排気ガス中に含まれる水蒸気が赤外線を吸収する
ため、その影響補正等の対策が必要であること、
ならびに測定セルが汚れ易く、その保守に手数を
要するほかこの方式は、原理的に発酵槽内の培養
液中の溶存炭酸ガス濃度を直接測定することがで
きないなどの問題点があつた。 Conventionally, non-dispersive infrared carbon dioxide concentration meters have often been used to measure the carbon dioxide concentration of fermenters and exhaust gas during culture, but the devices are expensive;
Since water vapor contained in exhaust gas absorbs infrared rays, measures such as correcting the effects of this are necessary;
In addition, the measurement cell is easily soiled and requires a lot of effort to maintain, and in principle, this method has problems such as the inability to directly measure the concentration of dissolved carbon dioxide in the culture solution in the fermenter.
隔膜式電極法による発酵工程における排気ガス
中の炭酸ガス濃度、あるいは培養液中の溶存炭酸
ガス濃度の測定は従来からも種々試みられていた
が、工程中の蒸煮滅菌操作で電極が高温に曝され
る結果、検出部のガラス電極の膜ガラスから溶出
するアルカリによつて、電極内部のガス吸収液が
アルカリ性に変質するばかりでなく、検出用ガラ
ス電極や比較電極の劣化が促進されるため、測定
は容易でなく、精度も良くない等、充分な成果を
収めることができなかつた。 Various attempts have been made to measure the carbon dioxide concentration in the exhaust gas or the dissolved carbon dioxide concentration in the culture solution during the fermentation process using the diaphragm electrode method, but the electrodes were exposed to high temperatures during the steam sterilization process during the process. As a result, the alkali eluted from the membrane glass of the glass electrode of the detection part not only changes the gas absorption liquid inside the electrode to alkalinity, but also accelerates the deterioration of the detection glass electrode and reference electrode. Measurement was not easy and the accuracy was not good, so it was not possible to achieve sufficient results.
本発明は、上記のような隔膜式ガス電極を、発
酵槽やそのガス排気管の通路に取付けて使用しう
るようにした電極支持装置を供することを目的と
する。 SUMMARY OF THE INVENTION An object of the present invention is to provide an electrode support device that can be used by attaching a diaphragm-type gas electrode as described above to a passage of a fermenter or its gas exhaust pipe.
本発明はこの目的を達するために、被検ガスを
透過し耐熱耐圧性を有するガス透過膜で一端側の
開口が閉じられ、他端側の開口から内部に隔膜式
ガス電極を挿入し隔膜式ガス電極の一端面に設け
た隔膜が前記ガス透過膜に接近した状態に隔膜式
ガス電極を保持しうるように形成した耐熱耐圧性
材料の筒からなる隔膜式ガス電極支持装置を構成
したものである。 In order to achieve this objective, the present invention has an opening at one end closed with a gas permeable membrane that permeates the gas to be detected and has heat and pressure resistance, and a diaphragm type gas electrode is inserted into the interior through the opening at the other end. A diaphragm-type gas electrode support device consisting of a tube made of a heat-resistant and pressure-resistant material formed so that the diaphragm-type gas electrode can be held in a state where the diaphragm provided on one end surface of the gas electrode is close to the gas permeable membrane. be.
図面に示した一実施例について本発明装置を説
明する。 The apparatus of the present invention will be described with reference to an embodiment shown in the drawings.
１はステンレス鋼管等の耐熱耐圧性材料からな
る両端が開口した筒である。筒１の一端側の開口
は、多孔質ポリ四フツ化エチレン樹脂膜、シリコ
ーンゴム膜のような被検ガスの透過度の大きい膜
と、合成繊維の不織布などを交互に積層して形成
された被検ガスを透過し耐熱耐圧性を有するガス
透過膜２によつて閉じられている。この膜２は例
えばパツング等を介し袋ナツト３で筒１に取付け
るか、周縁を筒状に形成し、バンド等によつて筒
１に取付けられる。 Reference numeral 1 denotes a cylinder made of a heat-resistant and pressure-resistant material such as a stainless steel pipe and opened at both ends. The opening at one end of the tube 1 was formed by alternately laminating membranes with high permeability to the gas to be detected, such as porous polytetrafluoroethylene resin membranes and silicone rubber membranes, and nonwoven fabrics made of synthetic fibers. It is closed by a gas permeable membrane 2 that transmits the gas to be detected and has heat and pressure resistance. This membrane 2 is attached to the tube 1 with a cap nut 3 through a padding or the like, or the membrane 2 is formed into a tube shape at the periphery and attached to the tube 1 with a band or the like.
４は隔膜式ガス電極で、一端面に設けた隔膜５
がガス透過膜２の内面に接近するように、例えば
電極他端のキヤツプ１０を筒１の他端内側に設け
た段１３に接し、袋ナツト１１をキヤツプ１０の
外側からキヤツプ１０を囲んで筒１に締めつける
ことにより、隔膜式ガス電極４を筒１内に保持で
きるようにしてある。１２は隔膜式ガス電極のリ
ード線でキヤツプ１０から引き出したもの、６は
隔膜式ガス電極４の外筒、７は内部ガラス電極、
８は外筒６内に注入したガス吸収液、９は同じく
比較電極である。 4 is a diaphragm type gas electrode, with a diaphragm 5 provided on one end surface.
For example, the cap 10 at the other end of the electrode is brought into contact with the step 13 provided inside the other end of the tube 1, and the cap nut 11 is inserted from the outside of the cap 10 around the cap 10 so that the cap 10 approaches the inner surface of the gas permeable membrane 2. 1, the diaphragm gas electrode 4 can be held within the cylinder 1. 12 is a lead wire of a diaphragm type gas electrode drawn out from the cap 10, 6 is an outer cylinder of the diaphragm type gas electrode 4, 7 is an internal glass electrode,
8 is a gas absorption liquid injected into the outer cylinder 6, and 9 is a reference electrode.
この装置は発酵槽あるいはガス排気管の壁１４
に設けた孔１５に、筒１をガス透過膜２が内部に
位置するように途中まで挿入し、筒１の外面中央
部に設けたフランジ１７等を袋ナツト１６等によ
つて壁１４に取付けて使用するものである。 This device consists of a wall 14 of the fermenter or gas exhaust pipe.
Insert the tube 1 halfway into the hole 15 provided in the hole 15 so that the gas permeable membrane 2 is located inside, and attach the flange 17 etc. provided at the center of the outer surface of the tube 1 to the wall 14 with a cap nut 16 etc. It is used for
そして発酵槽の蒸煮滅菌操作時には、隔膜式ガ
ス電極４を筒１より取外し、筒１は装着したまゝ
で行なう。このときガス透過膜２は発酵槽内部等
の高温、高圧に十分耐えうると共に、測定対象ガ
ス以外の物質、菌類等を遮断し、透１内への侵入
を防ぎ、筒１内から発酵槽内等への外部物質、雑
菌等の侵入も防ぐ。 When the fermenter is sterilized by steaming, the diaphragm gas electrode 4 is removed from the tube 1, and the tube 1 is left attached. At this time, the gas permeable membrane 2 can sufficiently withstand the high temperature and high pressure inside the fermenter, etc., and also blocks substances other than the gas to be measured, such as fungi, and prevents them from entering the membrane 1. It also prevents the intrusion of external substances and bacteria.
発酵槽での発酵操作時には、隔膜式ガス電極４
を筒１内に前述のように取付て測定を行なう。 During fermentation operation in a fermenter, the diaphragm gas electrode 4
was installed in the cylinder 1 as described above and measurements were taken.
なお、蒸煮滅菌操作中、筒１の他端側の開孔に
は、盲蓋を被せておけば、万一ガス透過膜２が破
損した場合の事故防止に役立ち、また筒１内に電
導度検出電極を装置しておけば、異常を検知する
ことができる。 During the steam sterilization operation, if the opening at the other end of the tube 1 is covered with a blind cover, it will help prevent accidents in the event that the gas permeable membrane 2 is damaged, and it will also help prevent electrical conductivity inside the tube 1. If a detection electrode is installed, abnormalities can be detected.
発明者の実験によれば、
(1) 隔膜式ガス電極４として、シリコーンゴム膜
を隔膜５とする一般用炭酸ガス電極を用いて、
CO2標準ガス中に隔膜式ガス電極４のみを置い
たときの指示応答特性をみると、第２図のよう
になる。すなわち、隔膜式ガス電極直接では被
検ガスに電極受感部である隔膜５を被曝後約２
分で安定した指示応答し、被検ガス中より電極
を取り出した場合、同じく２分程度で指示は復
帰することを示している。 According to the inventor's experiments, (1) Using a general carbon dioxide gas electrode with a silicone rubber membrane as the diaphragm 5 as the diaphragm gas electrode 4,
The indication response characteristics when only the diaphragm gas electrode 4 is placed in CO 2 standard gas are shown in FIG. 2. In other words, when using a diaphragm type gas electrode directly, the diaphragm 5, which is the electrode sensing part, is exposed to the gas to be detected about 2 times after being exposed to it.
It is shown that if the electrode responds stably within minutes and the electrode is removed from the sample gas, the indication will return in about 2 minutes.
(2) 次に、この電極を、多孔性四フツ化エチレン
多孔質膜（厚み100μm）３枚、100μm厚テトロ
ン不織布２枚より成る積層膜（膜の厚さは５
Kg／cm2、140℃の耐圧、耐温度を有するように
設計した）を用意し、これを前述の筒１（ステ
ンレス鋼管製）に装着して、120℃にて１時間
蒸気加熱した後、筒１内に挿入装置して、(1)と
同じくCO2標準ガス中に筒１を被曝したときの
指示応答特性をみたところ、第３図のようにな
つた。その応答度は隔膜式ガス電極直接の場合
に比べて、若干の遅れがあるものの、実用上の
問題とならない程度であり、測定精度も良好で
あることが判明した。(2) Next, this electrode was attached to a laminated film consisting of three porous polytetrafluoroethylene porous membranes (thickness 100 μm) and two sheets of 100 μm thick Tetron nonwoven fabric (the membrane thickness was 5 μm).
Kg/cm 2 , designed to withstand pressure and temperature of 140°C) was prepared, installed in the aforementioned tube 1 (made of stainless steel pipe), heated with steam at 120°C for 1 hour, and then When the device was inserted into the cylinder 1 and the cylinder 1 was exposed to the CO 2 standard gas as in (1), the response characteristics were observed, as shown in Figure 3. Although there is a slight delay in response compared to the case of using a diaphragm type gas electrode directly, it was found that this was not a practical problem and the measurement accuracy was also good.
本装置の使用により、発酵槽内のガス濃度測定
の如く、電極が高温、高圧下に曝され、且つ電極
の装脱により槽内に夾雑物、雑菌等を持ち込むお
それがあつて、電極を装着後動かし得ない場合に
おける従来の問題点は解決され、ガス電極自体を
蒸煮滅菌操作する必要がないことから、その寿命
は著しく延長され、良好な精度で測定することが
できる等多大の利点を有する。 When using this device, the electrodes will be exposed to high temperatures and pressures, such as when measuring gas concentration in a fermenter, and there is a risk that removing the electrodes will introduce contaminants and germs into the tank. The conventional problems in cases where it cannot be moved later have been solved, and since there is no need to sterilize the gas electrode itself by steaming, its lifespan is significantly extended, and it has many advantages such as being able to measure with good accuracy. .
以上は発酵槽における炭酸ガス濃度の測定の場
合について述べたが、他の同種ガス電極について
も、適用し得ることは云うまでもない。 Although the above description has been made regarding the measurement of carbon dioxide concentration in a fermenter, it goes without saying that the present invention can also be applied to other similar gas electrodes.
第１図は本発明による電極支持装置の一実施例
の縦断面図であつて、隔膜式ガス電極を保持さ
せ、発酵槽内に突出するよう取付けた状態を示し
た図、第２図は隔膜式炭酸ガス電極（一般用）の
CO2標準ガス中における指示応答特性の実験例を
示す図、第３図は第２図に使用した電極を、本発
明による電極支持装置に装置して同じくCO2標準
ガスに対する指示応答特性を実験した例を示す図
である。
１…筒、２…ガス透過膜、３…袋ナツト、４…
隔膜式ガス電極、５…隔膜、６…外筒、７…内部
ガラス電極、８…ガス吸収液、９…比較電極、１
０…キヤツプ、１１…袋ナツト、１２…リード
線、１３…段、１４…壁、１５…孔、１６…袋ナ
ツト、１７…フランジ。
FIG. 1 is a longitudinal cross-sectional view of one embodiment of the electrode support device according to the present invention, showing a state in which a diaphragm-type gas electrode is held and installed so as to protrude into a fermenter, and FIG. Type carbon dioxide electrode (general use)
Figure 3 is a diagram showing an experimental example of indication response characteristics in CO 2 standard gas. The electrode used in Figure 2 was installed in an electrode support device according to the present invention, and the indication response characteristics in CO 2 standard gas were similarly tested. FIG. 1... cylinder, 2... gas permeable membrane, 3... bag nut, 4...
Diaphragm type gas electrode, 5...Diaphragm, 6...Outer tube, 7...Inner glass electrode, 8...Gas absorption liquid, 9...Comparison electrode, 1
0... Cap, 11... Cap nut, 12... Lead wire, 13... Step, 14... Wall, 15... Hole, 16... Cap nut, 17... Flange.
Claims (1)
透過膜で一端側の開口が閉じられ、他端側の開口
から内部に隔膜式ガス電極を挿入し隔膜式ガス電
極の一端面に形成された隔膜が前記ガス透過膜に
接近した状態に隔膜式ガス電極を保持しうるよう
に形成した耐圧、耐熱性材料の筒からなる隔膜式
ガス電極支持装置。1 The opening at one end is closed with a gas permeable membrane that allows the gas to be detected to pass through and has heat resistance and pressure resistance, and a diaphragm gas electrode is inserted inside through the opening at the other end, forming a diaphragm gas electrode on one end surface. A diaphragm-type gas electrode support device comprising a cylinder made of a pressure-resistant and heat-resistant material formed so that the diaphragm can hold the diaphragm-type gas electrode in a state where the diaphragm is close to the gas permeable membrane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57140527A JPS5930056A (en) | 1982-08-13 | 1982-08-13 | Diaphragm type gas electrode support apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57140527A JPS5930056A (en) | 1982-08-13 | 1982-08-13 | Diaphragm type gas electrode support apparatus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5930056A JPS5930056A (en) | 1984-02-17 |
| JPH0225460B2 true JPH0225460B2 (en) | 1990-06-04 |
Family
ID=15270735
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP57140527A Granted JPS5930056A (en) | 1982-08-13 | 1982-08-13 | Diaphragm type gas electrode support apparatus |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5930056A (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1154695B1 (en) | 1999-02-24 | 2005-12-21 | Danisco USA, Inc. | Betaine and bakery products |
-
1982
- 1982-08-13 JP JP57140527A patent/JPS5930056A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5930056A (en) | 1984-02-17 |
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