JPH0233096B2 - - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for testing abnormal blood by optically detecting abnormal blood based on the amount of light emitted.

It is well known that blood tests are a major factor in determining a person's health. BACKGROUND ART Conventionally, various methods for blood testing have been put into practical use. However, this type of test generally uses reagents and the like, and the method is complicated and it takes time to obtain results. Furthermore, it goes without saying that the need for blood transfusions has increased rapidly in recent years. At this time, it is dangerous to transfuse abnormal blood, and it is necessary to conduct a test in advance.
If it is discovered that there is some kind of abnormality in the blood before the blood is collected, it is extremely advantageous because there is no need to place an extra burden on the blood donor. However, it must be said that there has never been a method for easily and quickly determining abnormal blood.

This invention was made based on the above circumstances, and it measures the amount of extremely weak light emitted by the blood to be tested, and determines the correlation between the amount of light emitted by normal blood as a reference and detects an abnormality in a small amount of blood. It is an object of the present invention to provide a method for testing abnormal blood based on the amount of luminescence, which allows blood to be determined easily and quickly.

It is well known that many substances such as foods and polymer compounds emit extremely weak chemiluminescence as they deteriorate. It has been confirmed that human blood also emits extremely weak light. Furthermore, it was confirmed that there is a large difference in the amount of luminescence between the blood of a normal person and the blood of a diseased person. Therefore, it has been found that abnormal blood can be tested based on the correlation between the amount of luminescence of normal blood serving as a reference and the amount of luminescence of blood to be tested.

An embodiment of the present invention will be described below with reference to the drawings.

In FIG. 1, 11 is a container in which blood to be tested is stored. A photomultiplier tube 12 is provided opposite to the container 11 to detect weak light (visible light) generated from the blood to be tested. A shutter 13 is provided at an intermediate portion between the photomultiplier tube 12 and the container 11. These container 11, photomultiplier tube 12, and shutter 13 are housed in a dark box 14. The output signal of the photomultiplier tube 12 is converted into a pulse signal by a waveform shaping circuit 15 and supplied to a measuring section 16 . This measuring section 16 is composed of a counter or the like, and the pulse signal is counted by this counter. This counting output signal is supplied to a recording section 17, which is composed of, for example, a digital printer or a pen recorder, and is recorded therein.

In the above configuration, the container 11 contains approximately 2 ml of blood to be tested. This blood is actually kept at the same temperature as body temperature by a thermostat. In this state, the shutter 13 is opened for about 10 seconds, for example, and the photomultiplier tube 12 detects weak light generated from the blood to be tested. This detection signal is supplied to the measuring section 16 via the waveform shaping circuit 15, and the amount of light emission is counted. This counting output signal is
7 and recorded.

The luminescence amount of blood recorded in this way is compared with the luminescence amount of normal human blood measured in advance by the same method as described above, and abnormal blood is tested.

Figure 2 shows an example of measurement results using the above method, where A is a normal person, B is a diabetic patient, and C is a diabetic patient.
is the luminescence amount of the blood of a cancer patient and D is a leukemia patient. Each point differs depending on age and gender, but in the case of normal person A, the amount of luminescence is approximately 40 counts/10 seconds or less, and for each other affected person B, C, and D.
It is recognized that the amount of light emitted by is distributed over a range larger than that. Therefore, although it is difficult to determine the name of the disease, if there is any abnormality in the blood, the amount of luminescence will be higher than in a normal person, so abnormal blood can be tested.

In addition, since the photomultiplier tube 12 changes depending on the ambient temperature, etc., it is practically effective to perform the test by calculating the ratio of the dark count of the device, the count of normal human blood, and the count of the blood to be tested. be. According to this method, an average count value over 10 to 100 seconds is sufficient, and one test requires only about 1 to 2 minutes, allowing for rapid testing.

According to the above embodiment, abnormal blood is tested based on the amount of light emitted from the blood. Therefore, the time required for inspection can be significantly shortened compared to the conventional method.

Further, since the amount of blood to be tested is about 2 ml, less blood is required for the test, and the burden on the person collecting the blood can be reduced.

It should be noted that the present invention is not limited to the above-mentioned embodiments. For example, the dark count and the blood count of a normal person may be stored in advance in, for example, a microcomputer, and the microcomputer may be used to perform the test. The test time can be further shortened by determining the ratio between the blood count and the normal person's count, and outputting a normal signal if the ratio is less than a predetermined value, and an abnormal signal if it is more than that.

It goes without saying that various other modifications can be made without departing from the gist of the invention.

As detailed above, according to the present invention, the amount of extremely weak light emitted by the blood to be tested is measured,
It is possible to provide a method for testing blood based on the amount of light emitted by which abnormal blood can be tested simply and quickly using a small amount of blood based on the correlation with the amount of light emitted by normal blood as a reference.

[Brief explanation of drawings]

FIG. 1 is a block diagram showing an embodiment of a blood testing method based on luminescence intensity according to the present invention, and FIG. 2 is a diagram showing an example of measured values by this testing method. 11... Container, 12... Photomultiplier tube, 13...
...Shutter, 14...Dark box, 15...Waveform shaping circuit, 16...Measuring section, 17...Recording section.

Claims (1)

[Claims] 1 Abnormal blood is detected by measuring the amount of light emitted from blood to be tested contained in a test container, and detecting abnormal blood based on the correlation between this amount of light and the amount of light emitted from normal blood as a reference. How to test blood.