JPH0260306B2 - - Google Patents

Description

[Detailed description of the invention]

TECHNICAL FIELD The present invention relates to a beverage that has the effect of lowering or suppressing the increase in blood cholesterol. Beverages referred to in the present invention include soy milk, milk, fruit juice, soft drinks,
Refers to nutritional drinks, carbonated drinks, etc. The effect of lowering blood cholesterol here refers to the effect of lowering blood cholesterol concentration in those whose blood cholesterol concentration is higher than normal, and the effect of suppressing the increase in blood cholesterol refers to the effect of reducing blood cholesterol concentration in those who have higher than normal blood cholesterol concentration. It has the effect of suppressing the constant rise in blood cholesterol even when ingested. Based on a large amount of conventional knowledge, it is well known that polyunsaturated fatty acids (such as linoleic acid) and plant sterols in vegetable oils have the effect of lowering or suppressing the increase in blood cholesterol in humans and other animals. ing. In recent years, this type of substance has been put into practical use as a supplemented and fortified health food in foods or drinks for the purpose of improving nutrition. The present inventors focused on the presence or absence of physiological effects of oil and fat components other than the above-mentioned substances that have been clarified in the past,
As a result of various studies, we discovered that monolinolein, a type of monoglyceride, has the same effect on lowering and suppressing blood cholesterol levels as conventional substances at 1/100th the amount, and completed the present invention. did. That is, the present invention is a beverage containing 0.1% to 30% by weight of monolinolein. Monolinolein as used herein refers to α-monolinolein and/or β-monolinolein. In particular, β-monolinolein has excellent effects. There are no particular restrictions on the method for preparing monolinolein used in the present invention, and in addition to concentration or fractionation of natural monoglycerides, direct esterification and transesterification of glycerin using an alkali or a catalyst such as P-toluenesulfonic acid or an enzyme. Known synthetic methods including partial hydrolysis of triglyceride and diglyceride can be used, and combinations of these methods may also be used. The monolinolein obtained in this way can be used as it is or dissolved or dispersed in water or fats and oils, and can be added to a wide range of beverages, and even a small amount of it can lower blood cholesterol. It also greatly contributes to improving your eating habits because it suppresses the increase. According to the present invention, monolinolein is added in an amount of 0.1% to 30%, preferably 1% to 5%, in a water-based solution.
By dissolving or dispersing it, it is possible to produce a health food that has the effect of lowering or suppressing the increase in blood cholesterol. At this time, glycerin fatty acid esters other than monolinolein,
It is also possible to use emulsifiers such as sucrose fatty acid ester, sorbitan fatty acid ester, and lecithin. The inventors' previous application (Japanese Patent Application Laid-Open No. 57-35517,
JP-A-57-36935, JP-A-57-36936, JP-A-Sho
In addition to monolinolein, the effective organisms contained in the compositions and edible fats and oils of the invention described in Japanese Patent Application Laid-Open No. 57-39736 and JP-A No. 57-42631 contain large amounts of plant sterols and diglycerides, which are semi-solid. Therefore, even if it is included in fats and oils, a beverage with a favorable taste and flavor cannot be obtained. The content of diglyceride or plant sterol is preferably 5 times or less by weight relative to monolinolein. The present invention will be specifically explained below using Examples. Example 1 (1) Method for preparing monolinolein - Preparation from corn oil - After adding 10 kg of methyl ethyl ketone to 1 kg of refined corn oil and stirring, the mixture was cooled at -70°C for 3 hours using a dry ice/methanol refrigerant. Thereafter, methyl ethyl ketone was distilled off in an evaporator from the methyl ethyl ketone solution in which the solids were removed by suction filtration to obtain a red colored liquid. Add 10 times the weight of methyl ethyl ketone to this and use the same method again to remove the low-temperature solidifying substances.The methyl ethyl ketone is then distilled off to form a red, highly viscous liquid.
Obtained 25g. Furthermore, 22 g of this substance was sequentially developed with the solvents listed in Table 1 through a glass column with an inner diameter of 70 mm packed with 1500 g of activated "Wako Gel C-200", and after collecting each section, the solvent was distilled off.
Four columns of fractionated material were obtained as shown in Table 2.

【table】

[Table] The classification obtained here was determined using high performance liquid chromatography equipped with a Hitachi Gel 3053 column.
Methanol:isooctane:water=500:10:20
A portion corresponding to the retention time of the monolinolein standard product was collected. What was collected was monoglyceride mainly consisting of linoleic acid. The fractionation was repeated and about 100 mg of monolinolein (purity 95%) was obtained from the 7.6 g portion. (2) Animal experiment After feeding 28-day-old Sprague-Dohle male rats on commercially available feed for 2 days, they were divided into sections, with 10 rats in each section.
The mice had an average weight of 98.9±2.3 g and were raised for 5 days with free access to the test feed shown in Table 3.
For feeding water, add 0.1% of the 4th category shown in Table 2 to 100g of distilled water, 0.1% of monolinolein, or
0.3% (w/w), 5 mg emulsifier (“Tween80”)
It was prepared on the day of use and allowed to be taken ad libitum by processing it for 5 minutes at 5000 rpm using a Waring blender. After the end of rearing, blood was collected under ether anesthesia after fasting for 16 hours, and serum was collected using a conventional method. Total cholesterol in serum was measured using the kit “cholesterol B-Test Wako” manufactured by Wako Pure Chemical Industries, Ltd. and serum β.
- Lipoprotein is also produced by the company's Kittu ``β-
Each was measured using "Lipoprotein-Test Wako". The breeding results and serum total cholesterol and serum β-lipoprotein values are shown in Table 4.

【table】

[Table] 〓
Make it %.
Chiyokora D drops 0.01%

[Table] From the above results, the addition of monolinolein had no effect on average weight gain, average food consumption, or feed efficiency, and no difference was observed in the autopsy findings from the control group. Serum total cholesterol level and serum β
-A significant effect of monolinolein on lipoprotein levels was observed. Example 2 Fruit juice was obtained by adding 0.2% monolinolein to commercially available apple juice (manufactured by Zennoh Co., Ltd.). This apple juice had the same effect of lowering or suppressing the increase in blood cholesterol as the drink of Example 1. Example 3 Dissolve each ingredient in the following formulation in 1000 parts of sterile water.
It was packed into a 200ml container and used as a nutritional drink. Sugars 150 parts Mixed amino acids 5 parts Ethanol 15 parts Monolinolein 3 parts Tocopherol acetate 1 part This nutritional drink had the same effect of lowering or suppressing the rise of blood cholesterol as the drink of Example 1. Example 4 Dissolve sugar to make a sugar solution, add acidulant,
A syrup was prepared by adding fragrance and other raw materials, and 0.2% monolinolein was added to the syrup. This was packed into 200ml bottles, filled with water injected with carbon dioxide gas, and capped to produce a carbonated drink. Example 5 After pulverizing 2 kg of dehulled soybeans, 10 times the amount of boiling water was added, dispersed in a dispersion mill, and then brought into direct contact with steam in a pipeline to be rapidly heated to 120°C.
and heated for 30 seconds. This was centrifuged to remove okara to obtain soy milk. Monolinolein in this soy milk
0.5% was added and dispersed. This soymilk had the same effect of lowering or suppressing the increase in blood cholesterol as the drink of Example 1.

Claims (1)

[Claims] 1. A beverage containing 0.1% to 30% by weight of monolinolein. 2. The beverage according to claim 1, wherein the monolinolein is β-monolinolein.