JPH0326781B2 - - Google Patents
Info
- Publication number
- JPH0326781B2 JPH0326781B2 JP18636983A JP18636983A JPH0326781B2 JP H0326781 B2 JPH0326781 B2 JP H0326781B2 JP 18636983 A JP18636983 A JP 18636983A JP 18636983 A JP18636983 A JP 18636983A JP H0326781 B2 JPH0326781 B2 JP H0326781B2
- Authority
- JP
- Japan
- Prior art keywords
- reagent
- sample
- injection groove
- outlet
- carrier
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000003153 chemical reaction reagent Substances 0.000 claims description 69
- 238000002347 injection Methods 0.000 claims description 42
- 239000007924 injection Substances 0.000 claims description 42
- 238000000034 method Methods 0.000 claims description 6
- 238000007789 sealing Methods 0.000 claims 1
- 239000007788 liquid Substances 0.000 description 2
- 101100298222 Caenorhabditis elegans pot-1 gene Proteins 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1095—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers
- G01N35/1097—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers characterised by the valves
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Description
【発明の詳細な説明】
本発明は試料導入方法及び装置、就中プレラベ
ル用の試料導入方法及び装置に関するものであ
る。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method and apparatus for introducing a sample, particularly for a pre-label.
従来プレラベル化法に於てサンプルは試験管に
とり試薬を加えて反応させた後、分析装置にかけ
分析していた。このため分析には多量のサンプル
が必要となり微量では分析が出来なかつた。又高
価な試薬も多量に必要であつた。さらにその操作
も面倒であり殊に加温を要する場合には一層であ
つた。 In the conventional pre-labeling method, the sample was placed in a test tube, added with a reagent and reacted, and then placed in an analyzer for analysis. For this reason, a large amount of sample was required for analysis, and it was not possible to analyze a trace amount. Also, large amounts of expensive reagents were required. Furthermore, the operation is troublesome, especially when heating is required.
そこで本発明に於てはサンプルを直接分析装置
に注入出来る如く為しサンプルが微量でも分析で
きかつ試薬も小量で済み操作も極めて容易である
試料導入方法及び装置を提案せんとするもので、
方法については、試薬注入溝に試薬を満たしそこ
にサンプル注入口を介して直接サンプルを注入後
試薬注入溝を密封して反応させ次いで試薬注入溝
をサンプル分析系に連通させキヤリヤーにより導
入させることを特徴とし、装置については、コツ
クの固定部にその中心点の点対称位置に試薬導入
口と試薬導出口とをおよびキヤリヤー導入口とキ
ヤリヤー導出口とを設け、コツクの回動部に前記
導出入口と接続する試薬注入溝とキヤリヤー導通
溝とを設け、試薬注入溝が試薬導出入口に接続し
た際に試薬注入溝と連通するサンプル注入口を固
定部に設けたことを特徴とする。 Therefore, the present invention aims to propose a sample introduction method and device that allows the sample to be directly injected into the analyzer, allowing analysis of even a minute amount of sample, requiring only a small amount of reagents, and being extremely easy to operate.
The method is to fill the reagent injection groove with reagent, inject the sample directly into it through the sample injection port, seal the reagent injection groove and allow it to react, then connect the reagent injection groove to the sample analysis system and introduce it using a carrier. The apparatus is characterized in that the fixed part of the pot is provided with a reagent inlet and a reagent outlet, and a carrier inlet and a carrier outlet, at symmetrical positions with respect to the center point thereof, and the rotary part of the pot is provided with a reagent inlet and a carrier outlet. The present invention is characterized in that a reagent injection groove and a carrier conduction groove are provided, which are connected to the reagent injection groove, and a sample injection port is provided in the fixed part, which communicates with the reagent injection groove when the reagent injection groove is connected to the reagent outlet/outlet.
以下図面に示す一実施例により本発明を詳細に
説明すれば、１はコツクで、固定部２と回動部３
より構成され、回動部３はハンドルによる手動或
はモーター駆動により自動的に回動するようにし
てある。コツク１の固定部２は、第２図Ａに示す
ように、回動部３を挟持する二段構成のもので
も、また、第２図Ｂに示すように、一段構成のも
のでもよい。４は試薬導入口、５は試薬導出口
で、固定部２の中心点の点対称位置に穿設し夫々
試薬導入管６、試薬導出管７を接続してある。８
はキヤリヤー導入口、９はキヤリヤー導出口で、
固定部２に試薬導出入口４，５と同様に穿設し、
且つ試薬導出入口４，５とキヤリヤー導出入口
８，９とが直交線上に存在するように配置してあ
る。この直交線上に配置することは、装置構成を
簡素にし製造を容易にする利点があるが、必ずし
も直交線上に配置する必要はなく、ある程度の角
度を有すればよい。キヤリヤー導入口８はキヤリ
ヤー管１０を介してポンプ１１液槽１２に接続
し、キヤリヤー導出口９はカラム１３を介して分
析装置１４に接続してある。１５はサンプル注入
口で通常シリンジ挿通口を為し開口或はセプタム
等設置何れでもよい。該サンプル注入口１５は、
試薬注入溝１６が試薬導出入口４，５と接続した
際に試薬注入溝１６と連通する位置の固定部２に
設置する。試薬注入溝１６は、回動部３に略弓形
に穿設して形成される。１７はキヤリヤー導通溝
で、回動部３周面を管で接続するか、第２図Ｂに
示すように、試薬注入溝１６と略直交する方向
に、深さを異にして穿設してもよい。１８はシリ
ンジである。 The present invention will be explained in detail below with reference to an embodiment shown in the drawings. Reference numeral 1 denotes a lock, a fixed part 2 and a rotating part 3.
The rotating part 3 is configured to be rotated manually by a handle or automatically by driving a motor. The fixing part 2 of the pot 1 may be of a two-stage structure that holds the rotating part 3, as shown in FIG. 2A, or may be a one-stage structure, as shown in FIG. 2B. Reference numeral 4 denotes a reagent inlet, and 5 a reagent outlet, which are bored at points symmetrical to the center point of the fixing part 2, and are connected to a reagent inlet tube 6 and a reagent outlet tube 7, respectively. 8
is the carrier inlet, 9 is the carrier outlet,
Drill holes in the fixing part 2 in the same manner as the reagent inlets and outlets 4 and 5,
In addition, the reagent inlet/outlet ports 4, 5 and the carrier inlet/outlet ports 8, 9 are arranged so as to be on perpendicular lines. Arranging on the orthogonal line has the advantage of simplifying the device configuration and facilitating manufacturing, but it is not necessarily necessary to arrange on the orthogonal line, and it is sufficient to have a certain angle. The carrier inlet 8 is connected to a pump 11 and a liquid tank 12 via a carrier pipe 10, and the carrier outlet 9 is connected via a column 13 to an analyzer 14. Reference numeral 15 denotes a sample injection port, which normally serves as a syringe insertion port, and may be either an opening or a septum. The sample inlet 15 is
It is installed in the fixed part 2 at a position that communicates with the reagent injection groove 16 when the reagent injection groove 16 is connected to the reagent introduction/outlet ports 4 and 5. The reagent injection groove 16 is formed by perforating the rotating portion 3 in a substantially arcuate shape. Reference numeral 17 denotes a carrier conduction groove, which is connected to the circumferential surface of the rotating part 3 with a tube, or is bored at different depths in a direction substantially perpendicular to the reagent injection groove 16, as shown in FIG. 2B. Good too. 18 is a syringe.
次いで本方法及び装置の作動について説明する
と、回動部３を回動させ試薬注入溝１６を試薬導
入口４及び試薬導出口５に接続させる。続いて試
薬導入管６から試薬導入口４を介し試薬注入溝１
６に試薬を注入させる。この試薬注入の際に試薬
注入溝１６の形によつて再現性に影響を与えるこ
とがあり三ケ月型乃至弓形が有効である。斯して
試薬が試薬注入溝１６に充満したとき、シリンジ
１８によりサンプル注入口１５を介して試薬注入
溝１６にサンプルを注入する。この際のサンプル
としては0.1μ乃至10μ位がよく7.5μのルー
プを使用するとき0.5μ位が最適である。そこで
回動部３を回動させ試薬注入溝１６を試薬導入口
４、試薬導出口５及びサンプル注入口１５より離
間させる。この回動部３の回動には予め45゜の如
き回転角を設定しておくのが便利である。然ると
き試薬注入溝１６は密封され必要時所望時間放置
させ試薬とサンプルを反応させる。通常は室温で
反応させるが、場合により加熱することもある。
反応終了後、回動部３を回動させ試薬注入溝１６
をキヤリヤー導入口８及びキヤリヤー導出口９と
連通させる。この際ポンプ１１により液槽１２に
よりキヤリヤー管１０を介してキヤリヤーが挿入
され試薬注入溝１６中の試薬と反応したサンプル
がカラム１３を介して分析装置１４に挿入され分
析される。 Next, the operation of this method and apparatus will be described. The rotating part 3 is rotated to connect the reagent injection groove 16 to the reagent inlet 4 and the reagent outlet 5. Next, the reagent injection groove 1 is passed from the reagent introduction tube 6 through the reagent introduction port 4.
Step 6 injects the reagent. At the time of this reagent injection, the reproducibility may be affected by the shape of the reagent injection groove 16, so a crescent shape or an arcuate shape is effective. When the reagent injection groove 16 is filled with the reagent in this manner, the sample is injected into the reagent injection groove 16 through the sample injection port 15 using the syringe 18 . In this case, the sample should be around 0.1μ to 10μ, and when using a 7.5μ loop, around 0.5μ is optimal. Therefore, the rotating part 3 is rotated to separate the reagent injection groove 16 from the reagent inlet 4, the reagent outlet 5, and the sample inlet 15. It is convenient to set a rotation angle of 45 degrees in advance for the rotation of the rotating portion 3. At that time, the reagent injection groove 16 is sealed and allowed to stand for a desired time when necessary to allow the reagent and sample to react. The reaction is usually carried out at room temperature, but may be heated in some cases.
After the reaction is completed, rotate the rotating part 3 to fill the reagent injection groove 16.
is communicated with the carrier inlet 8 and the carrier outlet 9. At this time, a carrier is inserted through the carrier tube 10 by the liquid tank 12 by the pump 11, and the sample reacted with the reagent in the reagent injection groove 16 is inserted into the analyzer 14 through the column 13 and analyzed.
上記の如き本発明によれば、方法については、
試薬注入溝に試薬を満たしそこにサンプル注入口
を介して直接サンプルを注入後試薬注入溝を密封
して反応させ次いで試薬注入溝をサンプル分析系
に連通させキヤリヤーにより導入させることを特
徴とし、装置については、コツクの固定部にその
中心点の点対称位置に試薬導入口と試薬導出口と
をおよびキヤリヤー導入口とキヤリヤー導出口と
を設け、コツクの回動部に前記導出入口と接続す
る試薬注入溝とキヤリヤー導通溝とをに設け、試
薬注入溝が試薬導出入口に接続した際に試薬注入
溝と連通するサンプル注入口を固定部に設けたこ
とを特徴とするので、サンプルを直接注入して試
薬と反応かつ分析系に導入出来、サンプルが極微
量でも分析ができかつ試薬も微量で済む。従つて
従来不可能の分野の分析も可能となり然も操作は
極めて簡単であり、臨床化学での応用範囲は広い
等実用効果は著大である。 According to the present invention as described above, the method includes:
The apparatus is characterized in that a reagent injection groove is filled with a reagent, a sample is directly injected into the sample via a sample injection port, the reagent injection groove is sealed and reacted, and then the reagent injection groove is communicated with a sample analysis system and introduced by a carrier. For this, a reagent inlet and a reagent outlet, and a carrier inlet and a carrier outlet are provided in the fixed part of the cot at points symmetrical with respect to its center point, and a reagent connecting part to the said outlet and outlet is provided in the rotating part of the cot. The present invention is characterized in that an injection groove and a carrier conduction groove are provided in the fixed part, and a sample injection port that communicates with the reagent injection groove when the reagent injection groove is connected to the reagent outlet/outlet is provided in the fixed part, so that the sample can be directly injected. It can be reacted with reagents and introduced into the analysis system, allowing analysis to be performed even with extremely small amounts of samples, and requiring only a small amount of reagents. Therefore, it is possible to conduct analyzes in fields that were previously impossible, and the operation is extremely simple, and the practical effects are significant, with a wide range of applications in clinical chemistry.
第１図は本発明一実施例流路図でＡ〜Ｃの順に
操作工程を示す、第２図Ａ，Ｂは第１図Ａ要部Ａ
−Ａ従断面図である。
１……コツク、２……固定部、３……回動部、
１６……試薬注入溝。
Fig. 1 is a flow path diagram of one embodiment of the present invention, showing the operation steps in the order of A to C. Fig. 2 A and B are main parts A of Fig. 1 A.
-A is a secondary sectional view. 1...Kottoku, 2...Fixed part, 3...Rotating part,
16...Reagent injection groove.
Claims (1)
入口を介して直接サンプルを注入後試薬注入溝を
密封して反応させ次いで試薬注入溝をサンプル分
析系に連通させキヤリヤーにより導入させること
を特徴とする試料導入方法。 ２ コツクの固定部にその中心点の点対称位置に
試薬導入口と試薬導出口とをおよびキヤリヤー導
入口とキヤリヤー導出口とを設け、コツクの回動
部に前記導出入口と接続する試薬注入溝とキヤリ
ヤー導通溝とを設け、試薬注入溝が試薬導出入口
に接続した際に試薬注入溝と連通するサンプル注
入口を固定部に設けたことを特徴とする試料導入
装置。[Scope of Claims] 1. Filling the reagent injection groove with a reagent and injecting the sample directly into it through the sample injection port, then sealing the reagent injection groove and causing a reaction.Then, the reagent injection groove is communicated with the sample analysis system and introduced by a carrier. A sample introduction method characterized by: 2. A reagent inlet and a reagent outlet, and a carrier inlet and a carrier outlet, are provided in the fixed part of the pot at symmetrical positions with respect to its center point, and a reagent injection groove is provided in the rotating part of the pot, which connects with the outlet. and a carrier conduction groove, and a sample injection port that communicates with the reagent injection groove when the reagent injection groove is connected to the reagent introduction/outlet port is provided in the fixed part.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP18636983A JPS6078330A (en) | 1983-10-05 | 1983-10-05 | Sample introduction method and device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP18636983A JPS6078330A (en) | 1983-10-05 | 1983-10-05 | Sample introduction method and device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6078330A JPS6078330A (en) | 1985-05-04 |
| JPH0326781B2 true JPH0326781B2 (en) | 1991-04-11 |
Family
ID=16187170
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP18636983A Granted JPS6078330A (en) | 1983-10-05 | 1983-10-05 | Sample introduction method and device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6078330A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR200476735Y1 (en) * | 2013-12-12 | 2015-03-26 | 김유신 | lighting apparatus according to cline |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2001274864A1 (en) * | 2000-05-19 | 2001-12-03 | Cytomation, Inc. | A rapid multi-material sample input system |
-
1983
- 1983-10-05 JP JP18636983A patent/JPS6078330A/en active Granted
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR200476735Y1 (en) * | 2013-12-12 | 2015-03-26 | 김유신 | lighting apparatus according to cline |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6078330A (en) | 1985-05-04 |
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