JPH0352027B2 - - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a washing device for washing beads and containers reacted with specimens and reagents with washing water in blood tests performed using antibody-coated beads (reaction balls). Regarding improvements.

The cleaning device used for the above-mentioned blood test is the first one.
As shown in the figure, a test tube-shaped reaction vessel 20 in which beads 1, a specimen, and a reagent are introduced and reacted.
A mounting table 3 is installed on the machine body a so as to mount the titration plates 2, which are arranged in multiple rows vertically and horizontally.
A dispensing head 4 is provided above, and dispensing nozzles 40 are installed on the lower surface of the dispensing head 4 in correspondence with the reaction vessels 20 of the titration plate 2.
Then, the washing liquid is dispensed into each reaction container 20..., and the dispensed washing liquid is passed through the suction nozzle 41, which is arranged vertically on the lower surface of the dispensing head 4 in parallel with the dispensing nozzle 40... Although it is designed to attract
In order to remove the washing water from the multiple reaction vessels 20 lined up simultaneously and quickly, multiple suction nozzles 41 installed vertically on the lower surface of the dispensing head 4 are installed. The proximal end side of the dispensing head 4 is connected to a cavity provided in the dispensing head 4, and the cavity is connected to the communication path 42.
A suction device P with a large displacement such as a suction blower is connected to the suction image via the drain liquid reservoir 43, and the suction pressure of the suction device P causes each suction nozzle 41...
The cleaning liquid is suctioned at the same time.

By the way, the washing device A that looks like this
In this case, washing water is supplied to each reaction vessel 2 from the dispensing nozzle 40.
0..., the suction nozzle 41...
During the cleaning process in which the suction nozzles 41 are performing suction operation, the tip openings of the suction nozzles 41 are connected to the reaction vessel 20.
The washing water is immersed in the washing water inside the reactor vessel 20, and the washing water is sucked out, and the washing water is supplied from the dispensing nozzles 40 to the reaction vessel 20 through a lid 44 provided on the suction nozzle 41. When the opening is covered, the negative pressure generated in the reaction container 20 by the suction of the suction nozzle 41 causes the amount of the suction removed by the suction nozzle 41 to be removed from the dispensing nozzle 40 from the reaction container 2.
The beads 1 and the reaction vessel 20 are washed by taking the washing water into the vacuum nozzle, so the operation is extremely efficient. When the cleaning water in each reaction container 20 is sucked out by suction by 41, the tip opening of the suction nozzle 41 is moved above the surface of the cleaning water due to the lowering of the cleaning water level in the reaction container. As air comes out and air is sucked in together, the suction pressure is insufficient to loosen and suck up the cleaning liquid on the underside of the beads 1 at the bottom of the reaction vessel 20, and a small amount of air is sucked at the bottom of the reaction vessel 20. There is a problem that cleaning solution may be left behind.

The present invention was made in order to solve this problem, and it is possible to use a small suction device that has been used in the past without converting the suction device to one with a larger capacity. The purpose of the present invention is to provide a new means by which the residual liquid of the cleaning water can be sucked out together with the air.

In the present invention, as a means to achieve this object, a sealed valve chamber is provided between the suction nozzles arranged in parallel on the lower surface of the dispensing head and the suction port of the suction device. The proximal ends of the multiple suction nozzles are connected to one end of the valve chamber individually or for each block, and the other end of the valve chamber is connected and communicated to the suction port of the suction device. In the valve chamber, all the suction nozzles in series are connected to the suction port side of the suction device at once, and each suction nozzle or each block is sequentially connected to the suction port side of the suction device. The present invention proposes a washing device for testing blood, etc., which is equipped with a switching valve for switching between two states.

Next, embodiments will be described in detail with reference to the drawings. Note that the same reference numerals in the drawings as in the previous means are used for components having the same effect.

FIG. 2 is a partially cutaway side view of a blood test cleaning device A in which the present invention can be implemented;
is the machine body, 3 is a mounting table provided on the machine body a to place the titration plate 2, 4 is a dispensing head arranged above the mounting table 3 and installed on the machine body a, 40...
are dispensing nozzles arranged in parallel on the lower surface of the dispensing head 4; The suction nozzles P are provided in series, P is a suction device that provides a suction operation to the suction nozzles 41, and 5 is a valve chamber 5a (Fig. 3) provided between the suction device P and the suction nozzles 41. It shows the valve box.

The mounting table 3 has a cam mechanism 3 operated by a motor M1 provided in the inner cavity of a box-shaped body a.
The dispensing head 4 is supported by an elevating table 31 that is moved up and down by a motor M1, and is moved up and down so as to vary the relative distance to the dispensing head 4 under the control of a motor M1. This mounting table 3 is fixedly mounted on the machine body a when the dispensing head 4 is mounted on the machine body a so as to be movable up and down.

The titration plate 2 placed on the top surface of the mounting table 3 has a reaction vessel 20 formed in the shape of a test tube.
They were arranged vertically and horizontally so that there were four in the vertical direction (the longitudinal direction of the fuselage a, and the left-right direction in FIG. 2) and five in the horizontal direction, and they were connected together with a connecting member 2120 It is a regular titration plate with holes. (Fig. 4) Furthermore, the dispensing nozzles 40 and the suction nozzles 41 vertically installed on the lower surface of the dispensing head 4 are used for each reaction of the titration plate 2 set on the upper surface of the mounting table 3. They are arranged to correspond to the containers 20... Each of the dispensing nozzles 40 communicates with a communication chamber (not shown) formed inside the dispensing head 4 at its base end, and connects to the cleaning liquid tank via piping connected to the communication chamber. (not shown).

In addition, the suction nozzles 41... have their proximal ends penetrating the dispensing head 4 in the vertical direction.
Connection pipes 51 are provided on the upper wall 50 of the valve box 5, with tubes 45 protruding from the upper surface side and connected to each of the protruding ends 41a arranged in an arcuate row as shown in FIG. ..., and these connecting pipes 51... communicate with the upper end side, which is one end side of the valve chamber 5a formed in the valve box 5, so that the valve chamber 5
It communicates with the suction port side of the suction device P via a.

The suction nozzles 41 communicate with the valve chamber 5a as shown in FIG.
Inside, five communication chambers 4a are formed, corresponding to each row, in which four of the suction nozzles 41 arranged vertically and horizontally are brought together and communicated with each other. and five connecting pipes 4 each communicating with the five communication chambers 4a...
five connecting pipes 5, each of which is provided protruding from the upper surface of the dispensing head 4, and the connecting pipes 4b are provided on the upper surface of the valve box 5 in communication with the valve chamber 5a.
1 through the tubes 45, the multiple suction nozzles 41 are divided into a plurality of blocks w in which a desired number of suction nozzles 41 are gathered together by the communication chamber 4a, and each block w is Valve chamber 5
In some cases, it may be communicated with a.

As shown in FIGS. 3 and 4, the valve box 5 has a disk-shaped valve chamber 5a formed therein, and a switching valve is installed in the valve chamber 5a. A disk-shaped valve body 6 is rotatably mounted on a rotating shaft 60 provided in the center, and the upper surface of the valve body 6 and the ceiling surface of the valve chamber 5a are in watertight sliding contact, so that the valve chamber Connecting pipe 51 connected to the upper end side of 5a
... and the connecting pipe 70 connected to the lower end side of the valve chamber 5a is blocked. Then, the valve body 6 is connected to the rotating shaft 6.
By rotating the valve body 6 around 0, the notch 61 formed in the valve body 6 faces the opening at the inner end of the connecting pipe 51 which opens on the ceiling surface of the valve chamber 5a, thereby communicating with the opening. The notches 61 formed in the valve body 6 are connected to the connecting pipes 51 arranged in a row on the ceiling of the valve chamber 5a, as shown in FIG. A notch 61 is formed in the shape of a long hole in the rotational direction of the valve body 6 so as to create a communication state.
a, and the connecting pipes 51 arranged in parallel in an arc-shaped row.
A small-diameter notch 61b that communicates with one of the holes, and a gap longer than the length of the elongated notch 61a between them in the rotational direction.
The valve body 6 is formed at a position corresponding to the distance between the inner end opening of the connecting pipe 51 and the rotating shaft 60 on the peripheral edge of the valve body 6, so that the valve body 6 can be rotated about the rotating shaft 60. The long hole-shaped notch 61a connects the connecting pipe 51.
..., all of the suction nozzles 41... are in a state of communication with the suction device P, and the small diameter notches 61b are arranged in parallel with each inner end opening of the connecting pipes 51... By facing each pipe one by one, the connecting pipe 5
Suction nozzle 41 or suction nozzle 4 communicating with 1
The blocks w of 1... are sequentially switched to the suction device P.
It is arranged so that it is in communication with the and,
The rotation of the valve body 6 is performed by driving and rotating the rotating shaft 60 by a desired amount under the control of the operation of a motor M2 assembled on the lower surface side of the valve box 5.

The suction device P is a large displacement vacuum pump such as a turbo fan, and its suction port communicates with the drain reservoir 43 via a communication pipe 71.
3 communicates with the lower end of the aforementioned valve chamber 5a via a connecting pipe 70, thereby communicating with the valve chamber 5a.

Note that 61c is a notch formed in the same shape as the small-diameter notch 61b and provided at the rear of the notch 61b in the rotational direction, so that two suction operations are applied to each connecting pipe 51... be. Moreover, 44 is a lid provided on each suction nozzle 41 to close each opening of the reaction container 20 of the titration plate 2.

The embodiment device constructed in this manner operates as follows.

When washing the beads 1 that have been introduced into each reaction container 20 of the titration plate 2 placed on the mounting table 3 together with the sample and reagent and reacted, the valve chamber 5a is used.
The valve body 6, which is a switching valve, is provided inside the valve chamber 5a so that the elongated hole-shaped notch 61a is aligned with all the inner end openings of the connecting pipes 51 that are parallel to the ceiling surface of the valve chamber 5a. Rotate it to the opposing state position. As a result, the multiple suction nozzles 41 vertically disposed on the lower side of the dispensing head 4 are connected to the reaction vessels 20, respectively.
The washing water that is dispensed from the dispensing nozzle 40 into each reaction vessel 20 while the water is injected into the reactor is
The liquid is suctioned out all at once and collected in the drainage reservoir 43.

Next, when a predetermined amount of washing water is supplied and a predetermined washing is completed, the supply of washing water from the dispensing nozzle 40 is stopped, and the washing water remaining in each reaction vessel 20 is sucked out. When performing exclusion, motor M2
is actuated to rotate the valve body 6 in the valve chamber 5a to cut off the connection between the elongated notch 61a and each connecting pipe 51..., and to open a valve for each of the connecting pipes 51... Small diameter notch 61 provided in body 6
b are switched sequentially to establish communication. As a result, each suction nozzle 41 provided on the dispensing head 4...
are connected to the suction device P individually and block by block, so that each suction nozzle 41 or the suction nozzles 41 in the block are limited to the suction device P, and the suction nozzle 4
1, the suction pressure of the suction device P acting on the suction device P is increased several times to several orders of magnitude.

As explained above, the blood test cleaning device according to the present invention has a sealed valve chamber between the suction nozzles arranged in parallel on the lower surface of the dispensing head and the suction port of the suction device. The proximal ends of the suction nozzles in the series are connected to one end of the valve chamber individually or in blocks of a plurality of suction nozzles, and the other end of the valve chamber is connected and communicated with the suction port of the suction device. In the valve chamber, all of the multiple suction nozzles are communicated to the suction port side of the suction device at once, and each suction nozzle or each block is sequentially communicated to the suction port side of the suction device. Since the structure is equipped with a switching valve that switches between the states of The suction device performs suction operation all at once using the suction pressure of the suction device, and the suction nozzles installed in a series communicate with the suction device individually or in blocks of multiple suction devices. Since the suction pressure is concentrated and strongly acts on the suction nozzle, the cleaning process can be carried out efficiently, and the residual liquid in the reaction vessel can be removed by suction after the cleaning process is completed. But the suction device, it's capacity (capacity)
It is now possible to use a small suction device with a strong suction pressure to prevent washing water from remaining in the reaction vessel, without having to replace it with a larger one.

[Brief explanation of drawings]

FIG. 1 is a partially cutaway side view of a conventional blood test washing device, FIG. 2 is a partially cutaway side view of a blood test washing device in which the present invention can be implemented, and FIG. 3 is a partially cutaway side view of a conventional blood test washing device. FIG. 4 is a perspective view of the main part, and FIG. 5 is a plan view of the main part. Explanation of drawing symbols, A... Washing device, a... Body, P... Suction device, 1... Beads, 2... Titration plate, 20... Reaction container, 21... Connecting member, 3... Mounting table , 30... cam mechanism, 31...
Elevating table, 4...Dispensing head, 4a...Communication chamber, 4
b... Connection pipe, 40... Dispensing nozzle, 41... Suction nozzle, 41a... Projecting end, 42... Communication path,
43...Drainage reservoir, 44...Lid, 45...Tube, 5...Valve box, 5a...Valve chamber, 50...Top wall,
51... Connection pipe, 6... Valve body (switching valve), 60...
... Rotating shaft, 61, 61a, 61b, 61c ... Notch, 70 ... Connection pipe, 71 ... Communication pipe, M1,
M2...motor, w...block.

Claims (1)

[Claims] 1. A sealed valve chamber is provided between the suction nozzles arranged in parallel on the lower surface of the dispensing head and the suction port of the suction device, and the proximal ends of the multiple suction nozzles are provided at one end of the valve chamber. The other end of the valve chamber is continuously communicated with the suction port of the suction device, and all of the multiple suction nozzles are connected to each other individually or in blocks that are assembled together. The present invention is characterized by being equipped with a switching valve that switches between a state where all the suction nozzles or blocks are communicated with the suction port side of the suction device and a state where each suction nozzle or each block is sequentially communicated with the suction port side of the suction device. A cleaning device for testing blood, etc.