JPH0370475B2 - - Google Patents
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- Publication number
- JPH0370475B2 JPH0370475B2 JP60032527A JP3252785A JPH0370475B2 JP H0370475 B2 JPH0370475 B2 JP H0370475B2 JP 60032527 A JP60032527 A JP 60032527A JP 3252785 A JP3252785 A JP 3252785A JP H0370475 B2 JPH0370475 B2 JP H0370475B2
- Authority
- JP
- Japan
- Prior art keywords
- oil
- extractant
- bacterial cells
- genus
- fermentation method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 230000001580 bacterial effect Effects 0.000 claims description 34
- 238000000855 fermentation Methods 0.000 claims description 28
- 230000004151 fermentation Effects 0.000 claims description 28
- 235000019198 oils Nutrition 0.000 claims description 21
- 239000003921 oil Substances 0.000 claims description 20
- 238000000034 method Methods 0.000 claims description 16
- 238000000605 extraction Methods 0.000 claims description 15
- 239000011324 bead Substances 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 10
- 239000002994 raw material Substances 0.000 claims description 9
- 239000003349 gelling agent Substances 0.000 claims description 6
- 239000004359 castor oil Substances 0.000 claims description 5
- 235000019438 castor oil Nutrition 0.000 claims description 5
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 claims description 5
- 241000894006 Bacteria Species 0.000 claims description 3
- 235000019484 Rapeseed oil Nutrition 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 239000004006 olive oil Substances 0.000 claims description 3
- 235000008390 olive oil Nutrition 0.000 claims description 3
- 239000003549 soybean oil Substances 0.000 claims description 3
- 235000012424 soybean oil Nutrition 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 2
- 241000193403 Clostridium Species 0.000 claims description 2
- 229910052799 carbon Inorganic materials 0.000 claims description 2
- 230000002503 metabolic effect Effects 0.000 claims description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 23
- 239000002207 metabolite Substances 0.000 description 15
- 230000000813 microbial effect Effects 0.000 description 6
- 238000005192 partition Methods 0.000 description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 5
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 5
- 230000007423 decrease Effects 0.000 description 5
- 239000008103 glucose Substances 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 3
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- 235000010413 sodium alginate Nutrition 0.000 description 3
- 239000000661 sodium alginate Substances 0.000 description 3
- 229940005550 sodium alginate Drugs 0.000 description 3
- 231100000419 toxicity Toxicity 0.000 description 3
- 230000001988 toxicity Effects 0.000 description 3
- CRBJBYGJVIBWIY-UHFFFAOYSA-N 2-isopropylphenol Chemical compound CC(C)C1=CC=CC=C1O CRBJBYGJVIBWIY-UHFFFAOYSA-N 0.000 description 2
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 2
- 125000004432 carbon atom Chemical group C* 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- MWKFXSUHUHTGQN-UHFFFAOYSA-N decan-1-ol Chemical compound CCCCCCCCCCO MWKFXSUHUHTGQN-UHFFFAOYSA-N 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 239000008101 lactose Substances 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- 235000005985 organic acids Nutrition 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- WJQOZHYUIDYNHM-UHFFFAOYSA-N 2-tert-Butylphenol Chemical compound CC(C)(C)C1=CC=CC=C1O WJQOZHYUIDYNHM-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 241000235649 Kluyveromyces Species 0.000 description 1
- 241000588901 Zymomonas Species 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 239000003240 coconut oil Substances 0.000 description 1
- 235000019864 coconut oil Nutrition 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000008041 oiling agent Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000007218 ym medium Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Description
（産業上の利用分野）
本発明は原料と菌体とを接触させて発酵させ、
発酵液中に抽剤を添加してアルコール、有機酸
類、アセトン等の代謝産物を抽剤相に抽出して回
収する抽出発酵法の改良に関するものである。
（従来の技術）
グルコース等の原料を乳糖発酵性酵母、アルコ
ール発酵性酵母等の菌体と接触させて発酵させ、
エタノール等の代謝産物を工業的に得る工程にお
いては、発酵液中の代謝産物濃度が高まると菌体
の活性が低下し、エタノール等の生産性が阻害さ
れることが知られている。そこで生成された代謝
産物を発酵槽内から速やかに除去するために発酵
液中に抽剤を添加し、代謝産物を抽剤相に抽出さ
せることにより発酵槽内の代謝産物濃度を常に低
く保ち、菌体の活性低下を防ぐ抽出発酵法が発明
され、例えば特公昭58−3677号として提案されて
いる。ところが抽出効率の大きい抽剤、即ち抽剤
中の代謝産物濃度（Kg／Kg）／発酵液中の代謝産
物濃度（Kg／Kg）として定義される分配系数ｍの
大きい抽剤は菌体に対する毒性が強く菌体の生産
性を著しく低下させるため、止むを得ず分配係数
ｍが小さく菌体に対する毒性も小さいノルマル−
デシルアルコール（ｍ＝0.39）、オイレルアルコ
ール（ｍ＝0.24）、ノルマル−ドデシルアルコー
ル（ｍ＝0.37）等が抽剤として使用されており、
この場合には抽出効率が悪いために抽剤からの代
謝産物の回収に大きいエネルギを必要とするとい
う問題が残されていた。
（発明が解決しようとする問題点）
本発明はこのような従来の問題点を解決し、菌
体の生産性を阻害することなく分配係数の大きい
抽剤を使用することができ、高効率で代謝産物を
得ることができる抽出発酵法を目的として完成さ
れたものである。
（問題点を解決するための手段）
本発明は、発酵槽内で原料と菌体とを接触さ
せ、得られた発酵液に抽剤を添加し代謝産物であ
る有機物を抽剤相に抽出して回収する抽出発酵法
において、菌体と炭素数18以上の油との混合液を
ゲル化剤を用いて菌体固定化ビーズとし、原料、
発酵液及び抽剤と菌体との接触を菌体固定化ビー
ズ中において油の存在下で行わせることを特徴と
するものである。
本発明において用いられる菌体としては、代表
的なアルコール発酵性酵母であるサツカロミセ
ス・セレビシエ（Saccharomyces cerevisiae）
協会７号等の菌体を応く用いることができ、この
ほかのサツカロミセス属やシゾサツカロミセス
属、クルイベロミセス属等の酵母や、ザイモモナ
ス属、クロストリデイウム属等の細菌を用いるこ
ともできる。これらの菌体は菌体濃度２％（体積
％以下同じ）以上、好ましくは10％以上の菌体培
養液とされ例えばゲル化剤であるアルギン酸ナト
リウム２％、酸化アルミナ10％の水溶液に油とと
もに混合されエマルジヨン化される。
本発明において用いられる油は溶剤の毒性から
菌体を保護する目的で用いられるもので、それ自
体が菌体に対して毒性を持たないこと、非水溶性
であること、常温において液状であること等の条
件を満足するものであればよいが、更に炭素数が
18以上であること、不飽和結合を持たないこと、
天然油であることが望ましい。炭素数が18未満の
油は菌体に対して毒性を示し、不飽和結合を持つ
油は使用中に不飽和部位が酸化されて物性に変化
を生ずる虞れがあるためである。これらの条件を
満足する油としては、例えばひまし油、オリーブ
油、大豆油、ヤシ油、なたね油等の植物油を挙げ
ることができ、このような油は固定化用溶液に対
して２％以上、好ましくは５％以上が添加され
る。次に菌体培養液と油とゲル化剤とのエマルジ
ヨンは例えばCaCl・2H2Oの２％水溶液である固
定化液中に滴下され、粒径が１〜２mm程度の寒天
状の菌体固定化ビーズとされる。
このようにして得られた菌体固定化ビーズは、
油の粒子と菌体とが至近距離にあるよう担体中に
固定されたものであり、例えば第１図に示される
発酵槽１１又は第２図に示される発酵槽２１中に
充填される。第１図に示される発酵法においては
グルコース等の原料が原料供給管１２から発酵槽
１１内へ供給されるとともに抽出タンク１３から
抽剤が抽剤供給管１４を経て発酵槽１１中に供給
され、発酵液と抽剤との混合液は排出管１５によ
り分離槽１６へ送られて発酵液と抽剤に分離され
る。この間に代謝産物であるエタノールは抽剤中
に抽出され、抽出タンク１３において代謝産物が
抽剤と分離されて管１７から取出される。また、
第２図に示される発酵法においては菌体固定化ビ
ーズは発酵槽２１の下部に充填され、抽出タンク
２２から抽剤供給管２３により供給される抽剤は
発酵槽２１の上部を流れつつ発酵により生じた代
謝産物を抽出し、抽出タンク２２へ戻る。いずれ
の方法による場合にも原料及び抽剤は菌体固定化
ビーズ中に浸透して菌体と接触することとなる
が、本発明においてはこの接触が油の存在下にお
いて行われ、油が菌体に対する抽剤の毒性を著し
く低下させるためにオルト−イソ−プロピルフエ
ノール（以下、OIPPと記す）やオルト−ターシ
ヤリー−ブチルフエノール（以下、OTBPと記
す）のような分配係数の高い抽剤を用いても菌体
の生産性低下をごくわずかにとどめることができ
る。従つて本発明によれば菌体の生産性を阻害す
ることなく分配係数の大きい抽剤が使用できるこ
ととなるが、その詳細な実験データは次の実施例
に示す。なお、上記の例ではゲル化剤としてアル
ギン酸ナトリウム、酸化アルミナ、塩化カルシウ
ムを使用したが、本発明の効果は固定化担体の種
類に依存しないので、その他の公知のゲル化剤を
自由に使用することができる。
実施例
菌体として前述のサツカロミセス・セレビシエ
協会７号を用い、この菌株をYM培地（グルコー
ス10Kg／m3）で培養して菌体懸濁液を得た。この
菌体懸濁液10％に対して10％のてんぷら油（大豆
油＋なたね油）を添加したもの、同量のオリーブ
油を添加したもの、同量のひまし油を添加したも
のの計３種類の混合液を調製し、各混合液をそれ
ぞれアルギン酸ナトリウム２％、酸化アルミナ10
％と混合したうえ、２％CaCl2・2H2O水溶液中
に滴下して菌体固定化ビーズとした。これら３種
類の菌体固定化ビーズをYM培養（ラクトース又
はグルコース50Kg／m3、CaCl25Kg／m3）で２〜
３日間活性化したのち、第１図に示す発酵槽１１
に入れ、10Kg／m3のラクトースと10Kg／m3のグル
コース原料を供給して24〜29時間発酵を行わせ
た。一方、抽剤としてはOIPP（ｍ＝1.5）と
OTBP（ｍ＝1.6）を用い、エタノール生産量を測
定した。この結果を第１表に示す。
第１表に示されるように、菌体としてサツカロ
ミセス・セレビシエ協会７号を使用したとき、抽
剤としてOIPPを用いると、油を用いない従来法
においてはエタノール生産量が4.4から0.05まで
低下するが、油を用いた本発明法においてはエタ
ノール生産量が0.5〜0.9まで回復することが分か
る。また、抽剤としてOTBPを用いると、従来
法においてはエタノール生産量が4.4から0.1まで
低下するが、本発明によれば3.7〜4.2まで回復す
ることが分かる。
次に油の含有率と本発明との関係を明らかにす
るため、菌体固定化ビーズ中のひまし油の含有量
を変化させて同様の試験を行つた。その結果を第
２表に示す。第２表から明らかなように、ひまし
油の含有率が５％を越えると、エタノール生産量
の顕著な回復が認められる。
(Industrial Application Field) The present invention involves fermenting raw materials by bringing them into contact with bacterial cells,
The present invention relates to an improvement in an extractive fermentation method in which an extractant is added to the fermentation liquid to extract and recover metabolites such as alcohol, organic acids, and acetone into the extractant phase. (Prior art) Raw materials such as glucose are brought into contact with bacterial cells of lactose-fermenting yeast, alcohol-fermenting yeast, etc., and fermented.
In the process of industrially obtaining metabolites such as ethanol, it is known that when the concentration of metabolites in the fermentation liquid increases, the activity of bacterial cells decreases and the productivity of ethanol etc. is inhibited. In order to quickly remove the metabolites produced in the fermenter from within the fermenter, an extractant is added to the fermentation solution, and by extracting the metabolites into the extract phase, the concentration of metabolites in the fermenter is always kept low. An extraction fermentation method that prevents a decrease in the activity of bacterial cells has been invented, and has been proposed, for example, in Japanese Patent Publication No. 58-3677. However, extractants with high extraction efficiency, that is, extractants with a large distribution system number m defined as the concentration of metabolites in the extract (Kg/Kg)/concentration of metabolites in the fermentation liquid (Kg/Kg), are toxic to bacterial cells. is strong and significantly reduces the productivity of bacterial cells, so it is unavoidable to use normal-type materials with a small partition coefficient m and low toxicity to bacterial cells.
Decyl alcohol (m = 0.39), eurel alcohol (m = 0.24), normal-dodecyl alcohol (m = 0.37), etc. are used as extractants.
In this case, the problem remains that a large amount of energy is required to recover metabolites from the extractant due to poor extraction efficiency. (Problems to be Solved by the Invention) The present invention solves these conventional problems, allows the use of an extractant with a large partition coefficient without inhibiting the productivity of bacterial cells, and provides a highly efficient extractant. It was completed with the aim of being an extractive fermentation method that can obtain metabolites. (Means for Solving the Problems) The present invention involves bringing raw materials into contact with bacterial cells in a fermenter, adding an extractant to the resulting fermentation liquid, and extracting organic substances, which are metabolic products, into the extract phase. In the extraction fermentation method, a mixture of bacterial cells and oil with a carbon number of 18 or more is made into beads with immobilized bacterial cells using a gelling agent, and raw materials,
This method is characterized in that the fermentation solution and extractant are brought into contact with the microbial cells in the presence of oil in microbial cell-immobilized beads. The bacterial cells used in the present invention include Saccharomyces cerevisiae, a typical alcohol-fermenting yeast.
In addition, yeasts such as Satucharomyces, Schizosatucharomyces, and Kluyveromyces, and bacteria such as Zymomonas and Clostridium can be used. You can also do that. These bacteria are grown in an aqueous solution containing 2% sodium alginate and 10% alumina oxide, which are gelling agents, together with oil, in a bacterial culture solution with a bacterial cell concentration of 2% or more (same below volume %), preferably 10% or more. Mixed and emulsified. The oil used in the present invention is used for the purpose of protecting bacterial cells from the toxicity of solvents, and must be non-toxic to bacterial cells, non-water soluble, and liquid at room temperature. It is fine as long as it satisfies the following conditions, but if the number of carbon atoms is
Must be 18 or more, have no unsaturated bonds,
Preferably natural oil. This is because oils with less than 18 carbon atoms are toxic to bacterial cells, and oils with unsaturated bonds may undergo oxidation of unsaturated sites during use, causing changes in physical properties. Examples of oils that satisfy these conditions include vegetable oils such as castor oil, olive oil, soybean oil, coconut oil, and rapeseed oil. % or more is added. Next, the emulsion of the bacterial cell culture solution, oil, and gelling agent is dropped into a fixing solution, such as a 2 % aqueous solution of CaCl. It is said to be a chemical bead. The bacterial cell-immobilized beads obtained in this way are
The oil particles and the bacterial cells are fixed in a carrier so as to be in close proximity, and are filled into, for example, the fermenter 11 shown in FIG. 1 or the fermenter 21 shown in FIG. 2. In the fermentation method shown in FIG. 1, a raw material such as glucose is supplied into the fermenter 11 from a raw material supply pipe 12, and an extractant is supplied from an extraction tank 13 through an extractant supply pipe 14 into the fermenter 11. The mixed liquid of the fermentation liquid and the extraction agent is sent to the separation tank 16 through the discharge pipe 15 and is separated into the fermentation liquid and the extraction agent. During this time, the metabolite ethanol is extracted into the extractant, and the metabolite is separated from the extractant in the extraction tank 13 and taken out from the tube 17. Also,
In the fermentation method shown in FIG. 2, the bacterial cell-immobilized beads are filled in the lower part of the fermenter 21, and the extract supplied from the extraction tank 22 through the extract supply pipe 23 flows through the upper part of the fermenter 21 and ferments. The metabolites generated are extracted and returned to the extraction tank 22. In either method, the raw materials and extraction agent penetrate into the microbial cell-immobilized beads and come into contact with the microbial cells, but in the present invention, this contact is carried out in the presence of oil, and the oil In order to significantly reduce the toxicity of extractants to the body, extractants with high partition coefficients such as ortho-iso-propylphenol (hereinafter referred to as OIPP) and ortho-tertiary-butylphenol (hereinafter referred to as OTBP) are used. However, the decrease in bacterial productivity can be kept to a very small extent. Therefore, according to the present invention, an extractant having a large partition coefficient can be used without inhibiting the productivity of bacterial cells, and detailed experimental data will be shown in the following example. In the above example, sodium alginate, alumina oxide, and calcium chloride were used as gelling agents, but since the effects of the present invention do not depend on the type of immobilization carrier, other known gelling agents may be freely used. be able to. Example The above-mentioned Satucharomyces cerevisiae Society No. 7 was used as a bacterial cell, and this bacterial strain was cultured in YM medium (glucose 10 Kg/m 3 ) to obtain a bacterial cell suspension. To this 10% bacterial suspension, there were three types of mixed solutions: one containing 10% tempura oil (soybean oil + rapeseed oil), one containing the same amount of olive oil, and one containing the same amount of castor oil. Prepare each mixture with 2% sodium alginate and 10% alumina oxide.
% and added dropwise to a 2% CaCl 2 .2H 2 O aqueous solution to obtain microbial cell-immobilized beads. These three types of bacterial cell-immobilized beads were cultured in YM (lactose or glucose 50Kg/m 3 , CaCl 2 5Kg/m 3 ) for 2 to 30 minutes.
After 3 days of activation, the fermenter 11 shown in FIG.
10 Kg/m 3 of lactose and 10 Kg/m 3 of glucose raw materials were supplied and fermentation was carried out for 24 to 29 hours. On the other hand, OIPP (m=1.5) is used as an extractant.
Ethanol production was measured using OTBP (m=1.6). The results are shown in Table 1. As shown in Table 1, when Satucharomyces cerevisiae association No. 7 is used as the bacterial cell and OIPP is used as the extractant, the ethanol production decreases from 4.4 to 0.05 in the conventional method that does not use oil. It can be seen that in the method of the present invention using oil, the ethanol production amount was recovered to 0.5 to 0.9. Furthermore, when OTBP is used as the extraction agent, the ethanol production decreases from 4.4 to 0.1 in the conventional method, but it is found that it recovers to 3.7 to 4.2 according to the present invention. Next, in order to clarify the relationship between the oil content and the present invention, similar tests were conducted by varying the castor oil content in the microbial cell-immobilized beads. The results are shown in Table 2. As is clear from Table 2, when the content of castor oil exceeds 5%, a remarkable recovery in ethanol production is observed.
【表】【table】
【表】【table】
【表】
（発明の効果）
本発明は以上の説明から明らかなように、菌体
を油とともに担体中に固定して発酵液及び抽剤と
菌体との接触を油の存在下で行わせることにより
OIPPやOTBPのような分配係数の大きい抽剤が
菌体に及ぼす毒性を緩和し、高効率でエタノール
等の代謝産物の生産を行わせることに成功したも
のである。よつて、本発明によれば分配係数が大
で代謝産物の回収が容易な抽剤を用いた抽出発酵
プロセスが実現でき、産業の発展に寄与するとこ
ろは極めて大きいものがある。
なお、本発明はエタノール等のアルコール発酵
のみならず、抽出発酵が有効であることが知られ
ているアセトン発酵や有機酸類の発酵にもそのま
ま適用することができることはいうまでもない。[Table] (Effects of the Invention) As is clear from the above description, the present invention allows bacterial cells to be immobilized in a carrier together with oil, and contact between the fermentation liquid and extractant and the bacterial cells is carried out in the presence of oil. possibly
This method succeeded in reducing the toxicity of extractants with large partition coefficients, such as OIPP and OTBP, on bacterial cells and allowing them to produce metabolites such as ethanol with high efficiency. Therefore, according to the present invention, an extractive fermentation process using an extractant with a large partition coefficient and easy recovery of metabolites can be realized, which greatly contributes to the development of industry. It goes without saying that the present invention can be applied not only to alcohol fermentation such as ethanol, but also to acetone fermentation and fermentation of organic acids, for which extractive fermentation is known to be effective.
第１図及び第２図は本発明の抽出発酵法を示す
フローシートである。
１１，２１：発酵槽、１３，２２：抽出タン
ク。
FIG. 1 and FIG. 2 are flow sheets showing the extraction fermentation method of the present invention. 11, 21: Fermentation tank, 13, 22: Extraction tank.
Claims (1)
た発酵液に抽剤を添加し代謝産物である有機物を
抽剤相に抽出して回収する抽出発酵法において、
菌体と炭素数18以上の油との混合液をゲル化剤を
用いて菌体固定化ビーズとし、原料、発酵液及び
抽剤と菌体との接触を菌体固定化ビーズ中におい
て油の存在下で行わせることを特徴とする抽出発
酵法。 ２ 油がひまし油、オリーブ油、大豆油、なたね
油等の天然油である特許請求の範囲第１項記載の
抽出発酵法。 ３ 菌体がサツカロミセス・セレビシエ協会７
号、サツカロミセス属、シゾサツカロミセス属、
クルイザイモモナス属、クロストリデイウム属か
ら選択されたものである特許請求の範囲第１項記
載の抽出発酵法。[Claims] 1. In an extractive fermentation method in which raw materials and bacterial cells are brought into contact in a fermenter, an extractant is added to the resulting fermentation liquid, and organic substances as metabolic products are extracted and recovered in the extract phase. ,
A mixture of bacteria and an oil having a carbon number of 18 or more is made into bacteria-immobilized beads using a gelling agent. An extraction fermentation method characterized by being carried out in the presence of 2. The extractive fermentation method according to claim 1, wherein the oil is a natural oil such as castor oil, olive oil, soybean oil, or rapeseed oil. 3 The bacterial body is Satucharomyces cerevisiae association 7
No., Satucharomyces genus, Schizosatucharomyces genus,
The extractive fermentation method according to claim 1, wherein the extract is selected from the genus Kluyzaimomonas and the genus Clostridium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60032527A JPS61192291A (en) | 1985-02-20 | 1985-02-20 | Method of extractive fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60032527A JPS61192291A (en) | 1985-02-20 | 1985-02-20 | Method of extractive fermentation |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2227854A Division JPH03108488A (en) | 1990-08-28 | 1990-08-28 | Production of alcohol by extractive fermentation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS61192291A JPS61192291A (en) | 1986-08-26 |
| JPH0370475B2 true JPH0370475B2 (en) | 1991-11-07 |
Family
ID=12361417
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP60032527A Granted JPS61192291A (en) | 1985-02-20 | 1985-02-20 | Method of extractive fermentation |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS61192291A (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2830689B2 (en) * | 1993-04-28 | 1998-12-02 | 東陶機器株式会社 | Water faucet |
| PT1239022E (en) * | 2001-03-09 | 2009-06-05 | Nestle Sa | OIL CONTAINING ONE OR MORE LONG-STRANDED POLYESATURATED FATTY (S) FROM BIOMASS, PREPARATION PROCESS, FOOD, NUTRITIONAL, COSMETIC OR PHARMACEUTICAL COMPOSITION CONTAINING THE SAME |
| US9303225B2 (en) | 2005-10-26 | 2016-04-05 | Butamax Advanced Biofuels Llc | Method for the production of isobutanol by recombinant yeast |
| CN101307337B (en) | 2008-05-09 | 2012-05-30 | 浙江工业大学 | A method for preparing coenzyme Q10 by coupling fermentation and extraction of Sphingomonas |
| US8828695B2 (en) | 2008-06-04 | 2014-09-09 | Butamax Advanced Biofuels Llc | Method for producing butanol using two-phase extractive fermentation |
| US9040263B2 (en) | 2010-07-28 | 2015-05-26 | Butamax Advanced Biofuels Llc | Production of alcohol esters and in situ product removal during alcohol fermentation |
| BR112012032172A2 (en) | 2010-06-18 | 2015-11-24 | Butamax Tm Advanced Biofuels | method to produce an alcohol and composition |
| US9605281B2 (en) | 2012-09-12 | 2017-03-28 | Butamax Advanced Biofuels Llc | Processes and systems for the fermentative production of alcohols |
| US9109196B2 (en) | 2012-09-12 | 2015-08-18 | Butamax Advanced Biofuels Llc | Processes and systems for the production of fermentation products |
| US9469584B2 (en) | 2013-03-15 | 2016-10-18 | Butamax Advanced Biofuels Llc | Method for producing butanol using extractive fermentation |
-
1985
- 1985-02-20 JP JP60032527A patent/JPS61192291A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS61192291A (en) | 1986-08-26 |
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