JPH0376884B2 - - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Field of Application] The present invention relates to a method for culturing Japanese cypress tree.
More specifically, it relates to a so-called primary culture method for logs from inoculation of logs with inoculum to the time when the first mushrooms are observed.

The method for cultivating edible mushrooms according to the present invention is optimal as a method for culturing edible mushrooms for producing mushrooms such as reishi mushrooms, shiitake mushrooms, oyster mushrooms, and maitake mushrooms.

[Technical background of the invention and conventional technology]

As the so-called primary culture method of sagebrush, the sterile culture method and the aseptic culture method are known. The sterile culture method is a method that does not take into account bacterial contamination.
This refers to a method of inoculating raw wood with inoculum and culturing it statically. In addition, the aseptic cultivation method refers to a method in which the logs are sterilized with moist heat and then inoculated with inoculum to prevent bacterial contamination of the logs, and then the logs are placed in a sterile space and cultured. However, until a few years ago, only the sterile culture method was used, and the period from inoculating the logs with the inoculum to seeing the first mushroom growth was aseptic. The previous method of culturing sagebrush had never been used. The aseptic culturing method was first adopted by the present inventor in the method for cultivating Sagittarius trees for the generation of Cinderaceae (Japanese Patent Publication No. 62-27769).

By the way, the above-mentioned aseptic culture method and aseptic culture method each have advantages and disadvantages. The bactericidal cultivation method has the advantage of allowing free aeration and water supply to the log base, but has the disadvantage that the logs are not sterilized with moist heat, making them less digestible, and that they float in the air. This method has the disadvantage that the survival rate of the inoculum is low because the log layer is easily contaminated by bacteria. In contrast, the above-mentioned patent application filed in 1983
According to the aseptic culture method adopted in No. 27769, the logs are sterilized with moist heat, making them highly digestible, and since the entire log is kept sterile, the log base will not be contaminated with bacteria. This has advantages, but on the other hand, it has the disadvantage that the logs are stored in a container, which tends to impede ventilation and water supply.

In view of the above-mentioned prior art, the present inventor has finally completed the present invention as a result of various research into culture methods that take advantage of the advantages of each of the sterile culture method and the aseptic culture method.

[Means for Solving the Problems] The most important cultivation method according to the present invention is to inoculate the upper end 2 of the log 1 which has been sterilized with moist heat, and then inoculate it with a plastic film 3 having flexibility and airtightness.
Cover the upper surface of the log 1 and the upper half of the circumference of the log 1, tie the plastic film 3 above the circumferential surface of the log 1 with a string 4, and immerse the lower half of the log 1 in the water storage part 6. It is characterized by being statically cultured for a certain period of time under such conditions.

The log 1 in the present invention may be a log used in the cypress culture method. For example, in the case of the Reishi mushroom cultivation method, logs obtained by cutting oak, oak, or other trees with a diameter of about 10 cm into rounds of about 10 to 20 cm in length may be used. This log is sterilized with moist heat according to conventional methods to protect it from bacterial contamination. The raw wood 1 that has been sterilized with moist heat in this manner is covered with a plastic film 3 to be described later, but in this case, it may be covered one by one or many pieces at once. The inoculum is inoculated into the top end 2 of the log 1 which has been sterilized by moist heat. 1 log to be covered with 3 plastic films
In the case of a book, the inoculum is directly inoculated into the upper edge 2 of the log 1. Also, if there are many logs 1,
Although the inoculum may be individually inoculated on the upper end 2 of each log 1, a thin medium layer 5 of a size that covers the upper end of all the logs is formed on the upper end 2 of the log 1, and then It is preferable to inoculate the inoculum. This is because by simply inoculating the seed fungus at an appropriate location on the thin medium layer 5, the upper end 2 of all logs 1 can be used as the seed fungus and the inoculation surface. The thin layer of the medium may be made of any material that can be ingested by the seed hyphae, such as paper or a thin plate. In addition, if necessary, nutrients preferred by mycelia may be absorbed or coated on them.

The plastic film 3 used in the present invention is required to have flexibility and airtightness. The plastic film 3 is required to have flexibility because
This is because it is necessary to cover the natural log 1, which has a wide variety of shapes, so as to approximately follow its outer shape. Also,
The reason why the plastic film 3 is required to be airtight is that it is necessary to prevent germs from entering through the plastic film 3 surface. Therefore, examples of the plastic film 3 used in the present invention include polyethylene film and polypropylene film. This plastic film 3 may be shaped like a wrapping cloth or a bag. This plastic film 3 covers the upper surface of the log 1 and the upper half of the circumference of the log 1. Here, the upper half of log 1 means log 1.
, it means the part above the part immersed in the water storage part 6, which will be described later. It is preferable that the hem of the skirt portion formed when the plastic film 3 is placed over the log 1 be as long as possible. This is because the air passage formed between the skirt part of the plastic film 3 and the skin of the log becomes longer, allowing clean air to be sent to the upper end of the log 1, which is the seed inoculation surface. It is from.

Next, the string-like material 4 used in the present invention will be explained. The material of this string-like object 4 is not particularly limited, and for example, wire or plastic string may be used. By tying the log 1 with such a string-like material 4, the upper end 2 of the log 1 inoculated with the inoculum can be held aseptically. In addition, it has the following effects. When there is only one log 1 covered with the plastic film 3, the inoculum inoculated onto the top end 2 of the log 1 can be prevented from falling by tying it up with a string 4. Furthermore, when there are a large number of logs 1 to be covered with the plastic film 3, by kneading them with the string-like material 4, the multiple logs can be handled as one, which improves work efficiency. be able to.

The culture method in the present invention is a static culture method.
In this case, the lower half of the log 1 is immersed in the water storage section 6 for static culture. Here, the lower half of the log 1 means the portion below the hem of the skirt portion of the plastic film 3 mentioned above.

Next, one embodiment of the present invention will be described based on the drawings. A water storage sheet 8 is placed on the bottom of the basket-like container 7,
A water storage portion 6 is formed by storing water in that portion. Then, a plurality of logs 1 each having a length of about 15 cm are set up in this water storage part 6. Top end 2 of each log 1
A thin medium layer 5 made of paper is placed to cover. A plastic film 3 is placed on top of it, and this is temporarily tied with a string-like material 4 above the circumferential surface of the log 1, and the whole is subjected to moist heat sterilization. After waiting for the cooling, the string-like material 4 is untied and the plastic film 3 is removed.
is removed once, and the inoculum is inoculated onto the thin medium layer 5. Thereafter, the plastic film 3 is quickly returned to its original position, tied again with the string 4, and left to stand for about 4 hours for static culture.

[Effect]

Since the present invention adopts the above configuration, the following effects are achieved.

In the method for cultivating Japanese cypress wood according to the present invention, the log 1 is sterilized by moist heat, so the digestibility of the log 1 is good.

In addition, the upper end of the log 1 is covered with a plastic film 3, and the circumferential surface of the log 1 is protected by skin, which prevents contamination by airborne bacteria, which is the main cause of contamination. be done.

Furthermore, since the upper part of the plastic film 3 that covers the upper half of the circumferential surface of the log 1 is tied with a string-like material 4, the following is formed between the skirt portion of the plastic film 3 and the skin of the log 1. As a result, the distance from the vent to the top end 2 of the log 1, which is the inoculum inoculation surface, becomes longer, and clean air can be sent to the inoculum inoculation surface.

Furthermore, since the lower half of the log 1 is statically cultured while being immersed in the water storage section 6, an optimal water supply can be obtained over time due to the capillary action of the log tissue.

〔effect〕

Since the present invention adopts the above configuration, the following effects can be obtained.

That is, the method for cultivating Japanese turmeric according to the present invention has the advantages of a sterile culture method in that the log has good digestibility and the log medium is not contaminated with bacteria, and the aeration and water supply to the log medium are not inhibited. It also has the advantages of the sterile culture method.

Therefore, the present invention is a method for cultivating Japanese staghorn that has high industrial value.

[Brief explanation of drawings]

FIG. 1 is a perspective view showing one embodiment of the method for cultivating sagebrush according to the present invention, FIG. 2 is a perspective view showing another embodiment of the method for cultivating sagebrush according to the present invention, and FIG. FIG. 3 is an exploded perspective view of FIG. 2; In the figure, 1...Log wood, 2...Top end, 3...Plastic film, 4...String-like material, 6...Water storage part.

Claims (1)

[Claims] 1 After inoculating the seed fungus into the top end 2 of the log 1 that has been sterilized by moist heat, cover the top surface of the log 1 and the upper half of the circumference of the log 1 with a flexible and airtight plastic film 3, and This method for cultivating Japanese cypress wood is characterized by tying a plastic film 3 with a string-like material 4 above the circumferential surface of a log 1, and statically culturing the log 1 for a certain period with the lower half of the log 1 immersed in a water storage part 6.