JPH0436661B2 - - Google Patents

Description

[Detailed description of the invention]

[Industrial Field of Application] The present invention relates to a feed additive that can simultaneously supplement lysine and methionine, which are amino acids that are often lacking in common feeds. [Prior Art] Lysine is currently widely used as a feed additive. Lysine is mainly used in the form of hydrochloride crystals, but methods have also been developed to use it in the form of free crystals or free aqueous solutions. On the other hand, 2-hydroxy-4-methylthiobutyric acid (hereinafter referred to as "MHA") is also called a methionine hydroxy analog, and is a compound having a hydroxyl group instead of the amino group of methionine. This substance can easily be converted into methionine in the body, so it is added to feed as a methionine substitute. It is difficult to obtain MHA as a crystal, and it is commercially available in the form of a concentrated aqueous solution or calcium salt. This equimolar crystal of lysine and MHA has already been developed and a patent application has been filed (Japanese Patent Application No. 59-24846;
(Japanese Patent Publication No. 169457/1983). [Problems to be Solved by the Invention] Since the hydrochloric acid moiety of lysine hydrochloride is essentially an unnecessary moiety as a feed additive, it is preferable to omit its use in terms of reducing costs. However, free crystals are difficult to crystallize, and the obtained crystals also tend to absorb carbon dioxide gas. Although there is a method to overcome these drawbacks (Japanese Unexamined Patent Publication No. 52-31020), it is expensive and undesirable as a feed manufacturing method. An aqueous solution in free form is undesirable because it becomes quite alkaline. On the other hand, when MHA is also added as a calcium salt, this calcium part is essentially unnecessary as a feed additive, so it is preferable to omit it if possible. In addition, the aqueous solution is strongly acidic with a pH of less than 1.0, so care must be taken when handling it.
Furthermore, it has a strong odor characteristic of sulfur-containing amino acids. [Means for Solving the Problems] As a result of various studies to solve the problems of early lysine and to develop a method of supplying cheaper lysine as a feed additive, the present inventors found that by combining it with MHA, The inventors have completed the present invention by discovering that the addition of both salts does not impair the feed effect in any way, and also solves both of the above-mentioned problems at once. That is, the present invention relates to a feed additive containing lysine MHA salt. Lysine and raw materials for producing lysine MHA salt
Both MHAs are used in free form. Lysine is L-
MHA may be L-form, DL-form, or DL-form.
It may be either D-form or DL-form. The molar ratio of lysine and MHA is not limited and can be arbitrarily set according to the requirements of the feed to be added. Generally, this molar ratio is within the range of 1/10 to 10/1, and in particular, is often used within the range of 1/4 to 4/1. The feed additive may be in the form of a solid, slurry, or aqueous solution. In the case of a solid, it may be in powder form, but from the viewpoint of hygroscopicity it is preferable to granulate it, and it is further preferable to crystallize it. In that case, lysine
Since the molar ratio of MHA crystals is 1:1, if the molar ratio required for feed additives is not 1:1, it is necessary to supplement the deficiency. Lysine may be supplemented in the form of hydrochloride crystals or free crystals, and MHA may be supplemented in the form of calcium salt crystals. In the case of an aqueous solution, usually an aqueous solution of free lysine and an aqueous solution of free MHA are mixed in a required molar ratio and concentrated if necessary. The pH of the aqueous solution can be set arbitrarily, but from the viewpoint of operability, a pH of about 4 to 9 is best, and it is usually within this range just by mixing. If necessary, add hydrochloric acid, sodium hydroxide, etc. to adjust the pH. The feed additive of the present invention can be commercialized by further adding various vitamins, minerals, etc., if necessary. [Action and Effect of the Invention] The feed additive of the present invention can supplement lysine and methionine, which are most important as limiting amino acids, all at once. In addition, hydrochloric acid added to lysine and calcium hydroxide added to MHA are no longer needed, and in addition to the cost of these themselves, the work burden of transporting and feeding them can be eliminated, reducing costs. can. The feed additive of the present invention is easy to manufacture, the product is easy to handle, and there is no problem with the odor peculiar to sulfur-containing amino acids associated with HMA. [Examples] Example 1 9,000 g of an 88% MHA aqueous solution ("ALIMET", manufactured by Monsanto) was mixed with 5,300 g of a 60% L-lysine aqueous solution to prepare 14,300 g of a mixed solution with a pH of 5.8. 100g of this solution contains 55g of MHA and 22g of L-lysine.
is included. 600 day-old broiler chicks were divided into 3 test plots, one of which was designated as the basic feed plot and fed a compound feed mainly consisting of corn and soybean meal. In addition, another group of the present invention was fed with the above-mentioned mixed feed to which the above-mentioned MHA-L-lysine mixed solution was added. Furthermore, the remaining one is a target area in which DL-methionine and L-methionine are added to the same above-mentioned mixed feed.
- A 7-week breeding test was conducted by feeding the mice with lysine hydrochloride crystals added to the mice so that the content of methionine and lysine was equivalent to that of the invention group. The composition of the basic feed is shown in Table 1, and the results are shown in Table 2.

【table】

[Table] As shown in Table 2 above, the chickens in the test group fed the feed according to the present invention had better weight gain and feed efficiency than those in the basic feed group, and the same performance as the control group. showed that. Example 2 MHA-L produced in the same manner as Example 1
- 1000 g of the lysine mixed solution was concentrated to obtain 850 g of a slurry with a pH of 5.8 and a slurry concentration of 30%. This slurry liquid
100g contains 65g MHA and 26g L-lysine. 600 day-old broiler chicks were divided into three test plots, one of which was designated as the basic feed plot, and fed with the formulated feed shown in Table 1, which mainly consisted of corn and soybean meal. Another group of the present invention was fed with the above-mentioned compounded feed to which the above-mentioned slurry liquid was further added. Furthermore, the remaining one was fed as a target group with the same above-mentioned mixed feed to which crystals of DL-methionine and L-lysine hydrochloride were added so that the content of methionine and lysine was equivalent to that of the invention group, and was fed for 7 weeks. A breeding test was conducted. The results are shown in Table 3.

[Table] As shown in Table 3 above, chickens in the test plot fed the feed according to the present invention had better weight gain and feed efficiency than those in the basic feed group, and were equivalent to those in the target group. Showed the results. Example 3 678 g of L-lysine was added to 1000 g of an 88% MHA aqueous solution ("ALIMET", manufactured by Monsanto). This corresponds to a molar ratio of MHA:L-lysine=1.3:1. The solution was concentrated 1.5 times and further heated to 20°C from 60°C.
The mixture was cooled to ℃, crystallized, and separated to obtain 640 g of crude crystals. Re-dissolve 64g of it in 300ml of water,
After adding 4 g of activated carbon and stirring at 60°C for 1 hour, the filtrate was concentrated 10 times, cooled from 60°C to 20°C for crystallization, and L-lysine 2-hydroxy-4- 6.0 g of methylthiobutyric acid 1:1 molar salt was obtained. The physical properties of this product are as follows. Elemental analysis:

[Table] Molecular weight: 296.43 (from elemental analysis C ₁₁ H ₂₄ O ₅ N ₂ S
Melting point: 169.5℃ Solubility in solvents: Easily soluble in water, slightly soluble in methanol/ethanol Crystal form: Needle crystals 100g of the above crude crystals contain L-lysine hydrochloride crystals
62.5 g was added to prepare a mixture of L-lysine and MHA in a molar ratio of 2:1. 600 day-old broiler chicks were divided into three test plots, one of which was designated as the basic feed plot, and fed with the formulated feed shown in Table 1, which mainly consisted of corn and soybean meal. Another group of the present invention was fed with the above-mentioned compounded feed to which the above-mentioned mixture was further added. Furthermore, the remaining one was prepared by mixing 2-hydroxy-4-methylthiobutyric acid calcium salt and L-lysine hydrochloride with the same above-mentioned compounded feed as a target group, so that the content of methionine and lysine was equivalent to that of the invention group. A 7-week breeding test was conducted by feeding the rats with the following ingredients: The results are shown in Table 4.

[Table] As can be seen in Table 4 above, chickens in the test plot fed the feed according to the present invention had better weight gain and feed efficiency than those in the basic feed group, and were equivalent to those in the target group. Showed the results.

Claims (1)

[Scope of Claims] 1. A feed additive containing lysine 2-hydroxy-4-methylthiobutyrate. 2. The feed additive according to claim 1, which is in the form of an aqueous solution. 3. The feed additive according to claim 1, which is in the form of powder or granules.