JPH0448202B2 - - Google Patents
Info
- Publication number
- JPH0448202B2 JPH0448202B2 JP7424584A JP7424584A JPH0448202B2 JP H0448202 B2 JPH0448202 B2 JP H0448202B2 JP 7424584 A JP7424584 A JP 7424584A JP 7424584 A JP7424584 A JP 7424584A JP H0448202 B2 JPH0448202 B2 JP H0448202B2
- Authority
- JP
- Japan
- Prior art keywords
- ring
- shaped
- objective lens
- lens
- refractive power
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 230000004907 flux Effects 0.000 claims description 9
- 230000003287 optical effect Effects 0.000 claims description 4
- 238000005286 illumination Methods 0.000 description 13
- 230000000694 effects Effects 0.000 description 4
- 230000004075 alteration Effects 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
- G02B21/08—Condensers
- G02B21/10—Condensers affording dark-field illumination
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Investigating Materials By The Use Of Optical Means Adapted For Particular Applications (AREA)
- Microscoopes, Condenser (AREA)
Description
【発明の詳細な説明】
(発明の技術分野)
本発明は、落射照明型顕微鏡における暗視野照
明装置、とくに所謂エピダーク用対物レンズに関
する。DETAILED DESCRIPTION OF THE INVENTION (Technical Field of the Invention) The present invention relates to a dark field illumination device for an epi-illumination type microscope, and particularly to a so-called epidark objective lens.
(発明の背景)
エピダーク照明とは、落射照明型顕微鏡におい
て対物レンズの周囲から対物レンズの光軸と同軸
のリング状光束を供給し、これを対物レンズの先
端付近に設けられたリング状集光部材によつて物
体面に導く暗視野照明である。最近、対物レンズ
の開口数及び作動距離が大きくなつてきており、
それに伴つて対物レンズのレンズ口径も大きくな
つてきている。このため従来のエピダーク照明系
では、対物レンズの周囲から十分な暗視野照明光
を供給することが難しくなつてきている。(Background of the invention) Epidark illumination is an epi-illuminated microscope in which a ring-shaped light beam coaxial with the optical axis of the objective lens is supplied from around the objective lens, and this is condensed into a ring-shaped light beam provided near the tip of the objective lens. This is dark field illumination that is guided to the object plane by a member. Recently, the numerical aperture and working distance of objective lenses have become larger.
Along with this, the lens aperture of the objective lens is also becoming larger. For this reason, in conventional epidark illumination systems, it has become difficult to supply sufficient dark-field illumination light from around the objective lens.
(発明の目的)
本発明の目的は、顕微鏡本体の構成をなんら変
更することなく、大きな口径を有する対物レンズ
においても十分なエピダーク照明を行うことが可
能で簡単な構成からなるエピダーク用対物レンズ
を提供することにある。(Objective of the Invention) The object of the present invention is to provide an objective lens for epidark which has a simple structure and can provide sufficient epidark illumination even with an objective lens having a large aperture without changing the structure of the microscope main body. It is about providing.
(発明の概要)
上記の目的を達成するために、本発明は、対物
レンズの先端付近に該対物レンズ光軸と同軸に配
置され、該対物レンズを包む如きリング状光束を
集光して物体面を証明するためのリング状集光部
材を有するエピダーク用対物レンズにおいて、前
記リング状集光部材と該対物レンズの鏡筒の入射
光側端との間に、前記リング状光束を外方へ屈折
させて前記リング状光束の径を拡大する負屈折力
のリング状中空レンズ部材を設けたものである。
このリング状レンズ部材は、対物レンズの入射光
側端に供給されるリング状光束をその負屈折力の
発散作用により外方へ屈折させてリング状光束の
リング径を拡大して、対物レンズの先端付近に設
けられたリング状集光部材に導く。従つて、対物
レンズの口径が大きなものであつてもリング状光
束を対物レンズの先端まで導くことができ、リン
グ状集光部材によつて物体面に十分な暗視野照明
光を供給することができる。(Summary of the Invention) In order to achieve the above object, the present invention is arranged near the tip of an objective lens coaxially with the optical axis of the objective lens, and condenses a ring-shaped light beam that wraps around the objective lens to create an object. In an epidark objective lens having a ring-shaped light condensing member for detecting a surface, the ring-shaped light beam is directed outward between the ring-shaped light condensing member and the incident light side end of the lens barrel of the objective lens. A ring-shaped hollow lens member having a negative refractive power is provided to enlarge the diameter of the ring-shaped light beam by refraction.
This ring-shaped lens member refracts the ring-shaped light flux supplied to the incident light side end of the objective lens outward by the diverging action of its negative refractive power, thereby expanding the ring diameter of the ring-shaped light flux, and The light is guided to a ring-shaped light condensing member provided near the tip. Therefore, even if the objective lens has a large aperture, the ring-shaped light beam can be guided to the tip of the objective lens, and the ring-shaped condensing member can supply sufficient dark-field illumination light to the object surface. can.
本発明においては、リング状光束の口径を拡大
するために、少なくとも負屈折力のリング状レン
ズ部材が必要であるが、この負屈折力リング状レ
ンズ部材と対物レンズ先端付近のリング状集光部
材との間に、さらに正屈折力のリング状レンズ部
材を設け、負屈折力リング状部材からの発散光束
を対物レンズ光軸と平行な平行光束に変換するこ
とが可能である。これによれば、対物レンズの周
囲のリング状光束の口径をむやみに拡大すること
が無く、対物レンズの長さが大きい場合にも、リ
ング状光束を収容するための対物レンズ全体の口
径を余り大きくする必要が無くなり、コンパクト
なエピダーク用対物レンズを構成することが可能
となる。 In the present invention, in order to expand the aperture of the ring-shaped light beam, at least a ring-shaped lens member with a negative refractive power is required, and this ring-shaped lens member with a negative refractive power and a ring-shaped condensing member near the tip of the objective lens are required. It is possible to further provide a ring-shaped lens member with a positive refractive power between the two and convert the diverging light beam from the ring-shaped member with a negative refractive power into a parallel light beam parallel to the optical axis of the objective lens. According to this, the aperture of the ring-shaped light beam around the objective lens is not unnecessarily expanded, and even when the length of the objective lens is large, the aperture of the entire objective lens to accommodate the ring-shaped light beam is not unnecessarily enlarged. There is no need to increase the size, and it becomes possible to construct a compact objective lens for epidark.
(実施例)
第1図は本発明によるエピダーク用対物レンズ
の第1実施例の構成を示す概略断面図である。図
示なき光源から供給されるリング状の照明光は、
顕微鏡本体内に斜設された孔開き反射鏡12によ
り反射され、対物レンズ10に向かう。そして、
対物レンズ10の入射光側端近傍にて対物レンズ
鏡筒11内に設けられた負屈折力のリング状レン
ズ部材1により発散され、対物レンズの先端付近
に設けられたリング状集光部材としてのリング状
凹面反射鏡2により、物体面Oに導かれる。負屈
折力リング状部材1は第2図の断面図Aと平面図
Bに示すごとく、中空のリング形状の負レンズで
あり、その内径は対物レンズの入射面の有効口径
よりやや大きめになつている。そして、負屈折力
リング状レンズ部材1に入射するリング状光束
は、ここでの発散作用を受けその口径が拡大され
てリング状凹面反射鏡2に達するため、リング状
凹面反射鏡の口径を大きくでき、対物レンズ10
の口径がかなり大きくても物体面Oには十分な暗
視野照明光を供給することが可能となる。(Example) FIG. 1 is a schematic sectional view showing the structure of a first example of an objective lens for epidark according to the present invention. Ring-shaped illumination light supplied from a light source (not shown) is
The light is reflected by an apertured reflecting mirror 12 provided obliquely within the microscope main body, and is directed toward the objective lens 10 . and,
The light is diverged by a ring-shaped lens member 1 with a negative refractive power provided in the objective lens barrel 11 near the end of the incident light side of the objective lens 10, and as a ring-shaped condensing member provided near the tip of the objective lens. It is guided to the object plane O by the ring-shaped concave reflecting mirror 2. As shown in the cross-sectional view A and the plan view B of FIG. 2, the negative refractive power ring-shaped member 1 is a hollow ring-shaped negative lens, and its inner diameter is slightly larger than the effective aperture of the entrance surface of the objective lens. There is. The ring-shaped light flux incident on the negative refractive power ring-shaped lens member 1 is subjected to a divergence effect here, and its aperture is enlarged to reach the ring-shaped concave reflector 2. Therefore, the aperture of the ring-shaped concave reflector is enlarged. Yes, objective lens 10
Even if the aperture is quite large, it is possible to supply sufficient dark field illumination light to the object plane O.
第3図は、本発明による第2実施例の概略構成
を示す断面図であり、第1図と同等の作用を有す
る部材には同一の図番を付した。この第2実施例
は負屈折力リング状レンズ部材3とリング状集光
部材としてのリング状凹面反射鏡5との間に、正
屈折力のリング状レンズ部材4を設けたものであ
る。ここで負屈折力リング状レンズ部材3及び正
屈折力リング状レンズ部材4はそれぞれ第4図
A,B及び第5図A,Bに示した如き形状を有し
ている。各図のAは断面図、Bは平面図である。
図示のごとく、正屈折力リング状レンズ部材4
は、負屈折力リング状レンズ部材3よりも大きな
口径を有しており、負屈折力リング状レンズ部材
3によつて発散されたリング状光束を受けて平行
光束に変換する。このため、このような第2実施
例の構成においては、正屈折力リング状レンズ部
材4からリング状集光部材としてのリング状凹面
反射部材5までのリング状光束の断面形状は一定
半径のリングとなつており、対物レンズの全体的
口径をこの間で一定に維持することが可能で、全
体としてコンパクトな構成とすることができる。
また、本実施例の如く負屈折力と正屈折力とのリ
ング状レンズ部材を組み合わせることによつて、
照明光束についての収差補正、特に色収差の補正
が可能となり、より良好な暗視野照明光束を供給
することが可能となる。 FIG. 3 is a sectional view showing a schematic configuration of a second embodiment of the present invention, and members having the same functions as those in FIG. 1 are given the same reference numbers. In this second embodiment, a ring-shaped lens member 4 having a positive refractive power is provided between a ring-shaped lens member 3 having a negative refractive power and a ring-shaped concave reflecting mirror 5 serving as a ring-shaped condensing member. Here, the negative refractive power ring-shaped lens member 3 and the positive refractive power ring-shaped lens member 4 have shapes as shown in FIGS. 4A, B and 5A, B, respectively. In each figure, A is a sectional view, and B is a plan view.
As shown, a positive refractive power ring-shaped lens member 4
has a larger aperture than the negative refractive power ring-shaped lens member 3, and receives the ring-shaped light beam diverged by the negative refractive power ring-shaped lens member 3 and converts it into a parallel light beam. Therefore, in the configuration of the second embodiment, the cross-sectional shape of the ring-shaped light beam from the ring-shaped lens member 4 with positive refractive power to the ring-shaped concave reflective member 5 as the ring-shaped condensing member is a ring with a constant radius. Therefore, the overall aperture of the objective lens can be kept constant during this period, and the overall configuration can be made compact.
Furthermore, by combining ring-shaped lens members with negative refractive power and positive refractive power as in this example,
It becomes possible to correct aberrations in the illumination light flux, particularly chromatic aberration, and to supply a better dark-field illumination light flux.
上記の各実施例に用いた負屈折力及び正屈折力
のリング状レンズ部材1,3及び4はそれぞれ第
2図、第4図及び第5図に示したごとく通常の負
レンズや正レンズの中心部を刳り貫いたものであ
るため、精度の高いものを比較的簡単に製作する
ことができるという利点を有している。尚、上記
の実施例では、リング状集光部材としてリング状
凹面反射部材を用いたが、これに限らずリング状
の中空正レンズを用いることも可能である。ま
た、リング状集光部材は必ずしも収斂作用を持つ
必要がなく、リング状光束を物体面へ集める作用
を有すればよく、照明実視野の大きさにより、例
えば対物レンズが低倍率の場合には円錐面の反射
鏡や発散作用を持つ凸面のリング状反射面とする
こともできる。 The ring-shaped lens members 1, 3, and 4 with negative refractive power and positive refractive power used in each of the above embodiments are similar to those of ordinary negative lenses and positive lenses, as shown in FIGS. 2, 4, and 5, respectively. Since the center is hollowed out, it has the advantage of being relatively easy to manufacture with high precision. In the above embodiment, a ring-shaped concave reflective member is used as the ring-shaped condensing member, but the present invention is not limited to this, and a ring-shaped hollow positive lens can also be used. Furthermore, the ring-shaped condensing member does not necessarily have to have a converging effect, but only has the function of concentrating the ring-shaped light beam onto the object plane. It can also be a conical reflecting mirror or a convex ring-shaped reflecting surface with a diverging effect.
(発明の効果)
以上の如く、本発明によれば、大口径の対物レ
ンズに対しても顕微鏡本体に何等変更を加えるこ
となく、対物レンズの周囲に供給されるリング状
光束を効率良く物体面に導くことが可能であり、
明るい暗視野照明を行うことができる。しかも、
負屈折力のリング状レンズ部材を用いているた
め、リング状光束の幅が大きくなり実視野の広い
低倍率対物レンズの場合に有利である。また、対
物レンズの先端部におけるリング状光束の口径
は、従来のものよりも大きくできるため、作動距
離を長くするためにも有利である。(Effects of the Invention) As described above, according to the present invention, even for a large-diameter objective lens, the ring-shaped light flux supplied around the objective lens can be efficiently transferred to the object surface without making any changes to the microscope body. It is possible to lead to
Bright darkfield illumination is possible. Moreover,
Since a ring-shaped lens member with negative refractive power is used, the width of the ring-shaped light beam becomes large, which is advantageous in the case of a low-magnification objective lens with a wide actual field of view. Furthermore, the aperture of the ring-shaped light beam at the tip of the objective lens can be made larger than that of the conventional one, which is advantageous for increasing the working distance.
第1図は本発明による第1実施例の概略構成を
示す断面図、第2図A,Bは第1実施例に用いら
れる負屈折力リング状レンズ部材の断面図及び平
面図、第3図は本発明による第2実施例の構成を
示す概略断面図、第4図A,B及び第5図A,B
はそれぞれ第2実施例に用いられる負屈折力リン
グ状レンズ部材及び正屈折力リング状レンズ部材
の断面図と平面図である。
〔主要部分の符号の説明〕、1,3……負屈折
力リング状レンズ部材、2,5……リング状集光
部材、4……正屈折力リング状レンズ部材、10
……対物レンズ、O……物体面。
FIG. 1 is a sectional view showing a schematic configuration of a first embodiment according to the present invention, FIGS. 2A and B are a sectional view and a plan view of a negative refractive power ring-shaped lens member used in the first embodiment, and FIG. 3 4A, B and 5A, B are schematic cross-sectional views showing the configuration of the second embodiment according to the present invention.
These are a sectional view and a plan view, respectively, of a negative refractive power ring-shaped lens member and a positive refractive power ring-shaped lens member used in the second embodiment. [Description of symbols of main parts], 1, 3...Negative refractive power ring-shaped lens member, 2, 5... Ring-shaped condensing member, 4... Positive refractive power ring-shaped lens member, 10
...Objective lens, O...object surface.
Claims (1)
同軸に配置され、該対物レンズを包む如きリング
状光束を集光して物体面を照明するためのリング
状集光部材を有するエピダーク用対物レンズにお
いて、 前記リング状集光部材と該対物レンズの鏡筒の
入射光側端との間に、前記リング状光束を外方へ
屈折させて前記リング状光束の径を拡大する負屈
折力のリング状中空レンズ部材を設けたことを特
徴とするエピダーク用対物レンズ。[Scope of Claims] 1. A ring-shaped condensing member disposed near the tip of the objective lens coaxially with the optical axis of the objective lens, for condensing a ring-shaped light flux that wraps around the objective lens and illuminating the object surface. In the objective lens for epidark, the diameter of the ring-shaped light flux is expanded by refracting the ring-shaped light flux outward between the ring-shaped condensing member and the end of the incident light side of the lens barrel of the objective lens. An objective lens for epidark, characterized in that it is provided with a ring-shaped hollow lens member having a negative refractive power.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7424584A JPS60217325A (en) | 1984-04-13 | 1984-04-13 | Objective lens for epidark |
| US06/721,403 US4626079A (en) | 1984-04-13 | 1985-04-09 | Dark field illumination apparatus for epi-illumination system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7424584A JPS60217325A (en) | 1984-04-13 | 1984-04-13 | Objective lens for epidark |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS60217325A JPS60217325A (en) | 1985-10-30 |
| JPH0448202B2 true JPH0448202B2 (en) | 1992-08-06 |
Family
ID=13541580
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP7424584A Granted JPS60217325A (en) | 1984-04-13 | 1984-04-13 | Objective lens for epidark |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS60217325A (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63133045A (en) * | 1986-11-25 | 1988-06-04 | Matsushita Electric Works Ltd | Lighting fixture for inspection |
| DE102004034958A1 (en) * | 2004-07-16 | 2006-02-09 | Carl Zeiss Jena Gmbh | Arrangement for microscopic observation and / or detection in a light scanning microscope with linear scanning and use |
| DE102007031244B3 (en) * | 2007-07-05 | 2009-01-02 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Apparatus and method for performing static and dynamic scattered light measurements in small volumes |
| JP5671241B2 (en) * | 2010-02-21 | 2015-02-18 | 株式会社シード | Mold for annular medical device and annular medical device molded using this mold |
| LU92505B1 (en) * | 2014-07-22 | 2016-01-25 | Leica Microsystems | METHOD AND DEVICE FOR MICROSCOPICALLY EXAMINING A SAMPLE |
| JP7465669B2 (en) * | 2020-02-18 | 2024-04-11 | 株式会社エビデント | Darkfield Condenser |
-
1984
- 1984-04-13 JP JP7424584A patent/JPS60217325A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS60217325A (en) | 1985-10-30 |
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