JPH045139B2 - - Google Patents

Description

[Detailed Description of the Invention] [Industrial Application Field] This invention supplies a test sample to a biochemical analyzer, specifically an analysis slide equipped with an analysis element impregnated with a reaction reagent, and This invention relates to a device for chemically analyzing changes in color density by measuring changes in color density due to reactions.

[Prior Art] In general, when determining whether or not a specific component is contained in blood, its content, etc., an analysis slide equipped with an analytical element impregnated with a reaction reagent is used. The test sample to be tested is supplied dropwise and reacted with the test sample in a thermostatic reaction chamber, and the progress or result of the reaction is measured, for example, the change in color density due to the reaction, using an optical concentration analyzer. Devices for measuring and analyzing by means of methods and other means have been known.

However, in the above measurements, serum is used as the test sample, and the serum must be separated from whole blood using a centrifugal separator and supplied individually to the analysis slides with a pipette, which is cumbersome to handle. It was hot.

[Purpose of the invention]

This invention is intended to solve the above problem. By simply injecting whole blood before serum separation, serum can be separated from whole blood and automatically supplied to the analysis element of an analysis slide. The purpose is to provide chemical analysis equipment.

[Structure of the invention]

In order to achieve the above object, the present invention provides a disk in which holders capable of holding an analysis slide at the bottom and a blood container at the top are installed on the same circle, and intermittently rotates the disk at each installation angle of the holder. The blood container is connected to a driving means that can achieve a speed that allows centrifugal separation of serum from blood in the blood container, and continuous rotation at a speed that exceeds the speed, and the lower surface of the blood container is maintained in a closed state at the centrifugation speed, and centrifugation is performed. A serum dispensing port is provided with a liquid passage blocking means that moves in the opening direction when the speed is increased to a speed exceeding the above speed. It is equipped with a measuring means for measuring changes, etc.

〔Example〕

Next, the present invention will be described based on an embodiment shown in the accompanying drawings.

Reference numeral 1 denotes a disk, and holders 4 holding an analysis slide 2 on the bottom and a blood container 3 on the top are removably attached at equal positions on the disk 1. The disk 1 is connected to the upper end of the output shaft 51 of the drive means 5. The driving means 5 is adapted to be rotated intermittently depending on the angle at which the holder 4 is arranged, and is driven at a speed necessary to centrifuge serum from blood in the blood container 3, and at a speed exceeding that speed. It is now possible to increase the speed.

The disk 1 is surrounded by housings 9 and 9' which constitute a thermostatic chamber equipped with a heater (not shown) so that the blood in the blood container is maintained at around 37°C.

The holder 4 is configured such that protrusions 43a, 44a provided on its front and rear walls 43, 44 can be engaged with locking grooves 12, 13 formed on the outer and inner end surfaces of the recess 11 provided on the upper surface of the disk 1. There is (second
(see figure). In this case, the locking groove 1 on the outer end surface
2 is made shallow, the locking groove 13 on the inner end surface is made deep, and the length of the protrusion corresponds to the depth, then the difference in the insertion amount of the protrusion of the holder 4 can be used as shown in the fourth figure. The holder 4 can be easily attached and detached, and since the holder 4 after engagement is moved in the direction in which the centrifugal force is exerted when the disk 1 rotates at high speed, there is no danger of detachment due to the centrifugal force. Of course, the means for attaching the holder 4 is not limited to the above configuration. In the figure, reference numeral 14 denotes a through hole provided in the inner bottom surface of the recess 11, into which the photometric head 6 is inserted.

The analysis slide 2 is an analytical element impregnated with a specific reaction reagent according to the purpose of analysis (for example,
No. 21677, JP-A-57-101761, JP-A-57-
21 is sandwiched between the upper and lower mounts 22 and 23, and the upper mount 22 has a serum supply window 22.
a, each of the lower mounts 23 is provided with a photometric window 23a. The analysis slide holding frame 41 constructed in the holder 4 is a flat box-shaped frame with one side open, and the analysis slide 2 is mounted on the upper and lower surfaces of the frame.
U-shaped notches 41a, 41 for preventing the serum supply window 22a and the photometry window 23a from being blocked.
b is provided.

The blood container 3 has an oblong cylindrical container main body 31 made of glass or plastic, etc., and has a sock-like shape with a blood injection port 32 on the top surface of the base, and serum separated by centrifugal force is placed on the bottom surface of the container body 31 during high-speed rotation. A serum dispensing port 33 is provided for taking out the serum and dispensing it onto the analysis slide side. The dispensing port 33 is provided with a liquid passage blocking means 34 that opens when a centrifugal force greater than that necessary for centrifugal separation is applied, and closes when the centrifugal force disappears. The above-mentioned action of the liquid passage blocking means 34 can be achieved, for example, by using a band member 36 that fits the front and rear sides of the movable body 34' into grooves 35 provided in the circumferential direction on the upper surface of the container body 31, as shown in FIGS. 5 and 6.
This can be done by attaching it to the hook portions at both ends via four elastic bodies 37 and selecting the elastic force or return force of the elastic bodies 37. The liquid passage blocking means 34
can also be replaced by suitable means other than the above-mentioned examples.

Blood container holding means 4 configured in the holder 4
2 is a mounting member 42a attached to the upper surface of the analysis slide holding frame 41 of the holder 4 as shown in the third figure, and an opening 42 for exposing the blood injection port 32 to the outside.
b, and a lid 42c having a
c is hinged at its base end to the upper end of the rear wall of the holder 4, and its free end can be click-connected to the upper end of the front wall. Therefore, open the lid 42c and place the blood container 3 on the mounting member 42a,
When the lid 42c is closed, the blood container 3 is held with the serum dispensing port 33 on the bottom facing the serum supply window 22a of the analysis slide, as shown in the fourth figure.

Note that the holder 4 is not limited to the above structure as long as the analysis slide 2 and blood container 3 can be easily exchanged and a sufficient holding force can be obtained during high-speed rotation.

Next, the operation of the above embodiment will be explained.

First, the holder 4 holding the analysis slide 2 and blood container 3 is attached to a predetermined location on the disk 1, and then an appropriate amount of filtered whole blood to be analyzed is injected into the blood container 3. Of course, the blood container 3 into which blood has been injected in advance may be held in the holder 4 attached to the disk 1. After that, disk 1
When driven at high speed, the serum injected into the blood container 3 is centrifuged toward the base of the blood container 3 due to the difference in specific gravity. The time required for this centrifugation is about 10 to 15 minutes at a disk rotation speed of 3000 rpm. After that, accelerate the disk to about 5000 rpm. As a result, the liquid passage blocking means 34, which had been blocking the serum dispensing port 33 provided on the bottom of the blood container 3, operates as shown by the two-dot chain line in FIG. 6 due to the increased centrifugal force, and opens the extraction port. Thereafter, when the rotation is loosened, the liquid passage blocking means 34 opens, but during this time a part of the centrifuged serum leaks out and is supplied to the analysis element 21 through the window 22a of the analysis slide 2. The time required for this serum supply to be completed is about 10 seconds from the time when the disk 1 is accelerated, and the drive is stopped after this time has elapsed.

Thereafter, the disk 1 is driven intermittently by the drive means 5 to wait for the reaction time between the serum and the reaction reagent.
At the stop position, the disk 1 is moved downward by a vertical moving means (not shown) to bring the measuring element 21 of the analysis slide 2 into close contact with the photometric head 6, and measurements are sequentially performed. In the measurement using the photometric head 6, a photometric beam of a desired wavelength obtained from a light source such as a halogen lamp is passed through a lens and a filter, and the analytical element 21 is irradiated with the reflected light. This is generally done by transmitting the current to a current, converting it into a current, calculating it with a microcomputer, and displaying the value on a suitable display means.

〔Effect of the invention〕

As described above, the biochemical analyzer according to the present invention is provided with a disk in which holders capable of holding an analysis slide at the bottom and a blood container at the top are installed on the same circle, and the disks are attached to the holder intermittently at each installation angle. The blood container is connected to a driving means capable of rotation and continuous rotation at a speed at which serum can be centrifuged from blood in the blood container, and at a speed exceeding the speed, and the lower surface of the blood container is maintained in a closed state at the centrifugation speed. A serum dispensing port equipped with a liquid passage blocking means that moves in the opening direction when the speed is increased to a speed exceeding the centrifugation speed is provided, and the reaction of the analytical element that receives the dispensed serum is provided on the lower side of the disk. Since it is equipped with a measuring means to measure color changes, etc., just by injecting whole blood before serum separation into the blood container on the disk, the serum will be separated and the separated serum will be transferred to the analysis element on the analysis slide. It becomes possible to automatically supply the Therefore, there is no need to prepare a separate centrifugal separator as in the past, and the excellent effect is achieved in that handling of the test sample and analysis work can be made smoother and more efficient.

[Brief explanation of drawings]

The figures show an embodiment of the present invention; FIG. 1 is a perspective view of the main parts, FIG. 2 is a perspective view showing the means for attaching the holder to the disk, FIG. 3 is an exploded perspective view of the holder, and FIG. 4 is a perspective view of the main parts. is a cross-sectional view of the holder holding the analysis slide and blood container attached to the disk;
FIG. 5 is a sectional view of the blood container, and FIG. 6 is a bottom view showing the operation of the liquid passage blocking means of the serum dispensing port. 1...disk, 2...analysis slide, 3...
Blood container, 4... Holder, 5... Drive means, 6
...Photometering head, 31...Container body, 32...
Blood injection port, 33...Serum extraction port, 34...Liquid passage blocking means.

Claims (1)

[Claims] 1. A disk is provided in which holders capable of holding an analysis slide at the bottom and a blood container at the top are placed on the same circle. The blood container is connected to a driving means capable of continuous rotation at a speed capable of centrifugation and a speed exceeding the centrifugation speed, and the lower surface of the blood container is maintained in a closed state at the centrifugation speed, and the speed is increased to a speed exceeding the centrifugation speed. A serum dispensing port is provided with a liquid passage blocking means that sometimes moves in the direction of the opening, and a measuring means is provided on the underside of the disk for measuring color changes due to reactions of the analytical element that receives the dispensed serum. A biochemical analyzer characterized by: