JPH0466537B2 - - Google Patents

Description

[Detailed Description of the Invention] <Industrial Application Field> The present invention provides an anti-obesity food per se, an anti-obesity food additive, obtained from wheat containing digestive enzyme inhibitors by the most suitable and inexpensive method. The present invention relates to an anti-obesity wheat food obtained by mixing the same, as well as a mixed food with pectin, which has a high effect of inhibiting fat digestion and absorption.

<Prior art> Since the existence of a protein fraction in wheat that inhibits α-amylase was reported in the 1940s, analysis of this fraction (for example, three major groups of inhibitors were identified), Analysis of the protein structure and its applications have been studied.

Regarding applications, development of anti-diabetic agents and anti-obesity agents was considered. However, the first challenge was the toxicity of this fraction of wheat, such as diarrhea. This is due to the discovery of phytohemaeglutinin,
This analysis led to a breakthrough. Thus, a method for extracting a partially purified wheat fraction was devised.
The application of so-called starch Brodsker as an anti-obesity agent to humans ended in failure. Currently, the company's main focus is on the development of antidiabetic agents.

<Problems to be Solved by the Invention> The causes of the Shuger-Brotzker failure described above are still unknown, but (a) they did not restrict dicarbons and monosaccharides (glucose, fructose, etc.);
(b) Anti-obesity cannot be achieved simply by inhibiting the digestion and absorption of starch, and (c) there may be an adaptation of pancreatic α-amylase secretion.

<Means for Solving the Problems> The present inventor has given consideration not only to α-amylase inhibitors contained in wheat, but also to trypsin inhibitors, lipase inhibitors, and fat absorption inhibitors, and has determined the appropriateness of these substances. , we have been conducting research on appropriate mixing ratios. As a result of many years of efforts, we have succeeded in producing and developing anti-obesity wheat foods and food additives that are inexpensive and highly usable. That is, according to the present invention, a liquid composition obtained by further removing low molecular weight substances from a fraction obtained by removing phytohemagglutinin by heating from an aqueous extract of wheat flour or wheat bran, or evaporating this to dryness. anti-obesity foods, food additives, and various anti-obesity foods, food additives, and food additives, which are made of powder obtained from wheat bran, liquid compositions or powder mixtures from the flour sources and wheat bran sources, and compositions obtained by adding pectin thereto. Obesity mixed food is provided.

<Example> The present invention will be specifically described below with reference to production examples and usage examples (effect comparison examples) of the wheat food (additive) of the present invention.

Example 1 (Production method of water extract of wheat flour) Wheat flour was dissolved in 4 to 5 times the amount of water by weight, stirred at a low temperature (for example, 4° C.) for 4 to 5 hours, and then left at a low temperature overnight. Remove the supernatant and heat at 80°C for 30 minutes. After quenching this solution, it was allowed to stand overnight at a low temperature, and the supernatant was dried by an appropriate method (eg, spray dry method).

This powder consists of 18% protein by weight, 65% carbohydrate by weight, and the rest is ash and water, and it was revealed that it contains inhibitors of saliva, α-amylase, pancreatic juice α-amylase, and trypsin for protein resolution. .

However, it was found that the taste was bitter and the absorbency was extremely high, making it unsuitable for use.

Therefore, a membrane filter was used to remove inorganic substances, particularly magnesium and sulfate groups, and also to remove low molecular weight compounds with a molecular weight of 10,000 or less. After various trials and errors, a satisfactory result was reached.

Example 2 (Wheat bran water extraction method) Wheat bran is ground into fine particles. The particle size is fine. This was extracted with water according to the method of Example 1, and inorganic components and low molecular weight compounds with a molecular weight of 10,000 or less were removed using a membrane filter.

This extract has a mild α-
It showed amylase inhibitory effect and strong lipase inhibitory effect.

Example 3 (Effect of water extract of wheat flour on rats) A total of 10 male Wistar rats, 5 each of SPF, were used. Group 1 (control group) was fed powder chow (CleaC
-2), the administration group was fed the same diet containing 10% wheat flour water extract for 12 days. In order to show the results, the weight gain curve and food intake are shown in the attached Figure 1. It is clear that the weight gain of the treated group was significantly suppressed, even though there was no change in the amount of food intake.

Blood biochemical findings showed that there was no change in renal function or pancreatic function, but there was a tendency for liver function, especially GOT, to improve.

Example 6 (Effect of wheat flour aqueous extract on humans) 3 g of wheat flour aqueous extract was administered with each meal for one month to five male adults who were diagnosed as severely obese based on measurements of skin fat content, body weight, and height. A decrease in body weight of 7 to 2.5 kg was observed.

Blood biochemical findings suggested that there were no changes in renal or pancreatic function, that high blood sugar levels were reduced, and that liver function was normalized. However, neutral fat showed a tendency to increase.

Example 7 (Administration effects of wheat flour water extract (A) and wheat bran water extract (B)) A total of 10 Wistar male rats, 5 each of SPF, were used.
Each group of 5 animals received powdered feed (CleaC-2);
The animals were fed a powdered diet (CleaC-2) containing 10% of a 1:1 mixture of A:B. The breeding period was 15 days.

This time as well, weight gain in the administered group was significantly suppressed (P<0.01). Blood biochemical findings indicate renal function,
No impairment of pancreatic function was observed. Remarkable changes were a decrease in triglycerides, a decrease in total cholesterol (both significant at P<0.05), a tendency toward a decrease in GTP, and a clear decrease in GOT.

Example 8 (Effect of administration of wheat flour water extract (A) and pectin (B)) The same procedure as in Example 7 was carried out using feed containing powder at a mixing ratio of A:B=1:1 at a concentration of 10%. I conducted an experiment.

Although the experimental results were not as remarkable as in Example 7,
showed similar trends.

Example 9 (Wheat flour water extract for humans (A')
2.5 ml each of Concentrated A' and Concentrated B', a total of 5 ml, was taken with every meal, and the effects of administration were examined for one month. Adult males (3 subjects) and females (3 subjects) with moderate obesity were used. Weight decreased by an average of 5 kg for boys and 5.6 kg for women.

Blood biochemical findings showed no renal or pancreatic dysfunction. An improvement in liver function and a clear (significant at P<0.05) decrease in triglyceride levels were observed. Total cholesterol also showed a decreasing trend. Blood sugar levels generally showed a decreasing trend.

Example 10 Effect of a large amount of A'+B' mixed solution The effect of taking 5 ml each of A' and B' (total 10 ml) with each meal was investigated using five severely obese adult females.

After continuing to take the drug for one month, I lost an average of 6.5 kg.
Blood biochemical findings were the same as in Example 9.

[Brief explanation of the drawing]

FIG. 1 is a graph showing the results of a rat rearing test using a wheat flour water extract-containing feed.

Claims (1)

[Claims] 1. A liquid composition obtained by removing substances with a molecular weight of 10,000 or less from a fraction obtained by removing phytohemagglutinin from a water extract of wheat flour by heating, or further evaporating this to dryness. An anti-obesity wheat food and food additive consisting of powder obtained by 2. The anti-obesity wheat food and food additive according to claim 1, wherein an aqueous extract of wheat bran is used instead of the aqueous extract of wheat flour. 3. A liquid composition obtained by mixing the water extracts according to claims 1 and 2 at a weight ratio of 1:10 to 10:1, or a powder obtained by further evaporating this to dryness. An anti-obesity wheat food and food additive consisting of: 4. An anti-obesity product comprising a liquid obtained by mixing the liquid composition according to claim 1 and pectin at a weight ratio of 1:10 to 10:1, or a powder obtained by further evaporating this to dryness. wheat food and food additives.