JPH0481741B2 - - Google Patents
Info
- Publication number
- JPH0481741B2 JPH0481741B2 JP61163009A JP16300986A JPH0481741B2 JP H0481741 B2 JPH0481741 B2 JP H0481741B2 JP 61163009 A JP61163009 A JP 61163009A JP 16300986 A JP16300986 A JP 16300986A JP H0481741 B2 JPH0481741 B2 JP H0481741B2
- Authority
- JP
- Japan
- Prior art keywords
- sensor
- adhesive
- circuit board
- printed circuit
- chip
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000000853 adhesive Substances 0.000 claims description 25
- 230000001070 adhesive effect Effects 0.000 claims description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 239000004202 carbamide Substances 0.000 claims description 12
- 102000004190 Enzymes Human genes 0.000 claims description 9
- 108090000790 Enzymes Proteins 0.000 claims description 9
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 6
- YCKRFDGAMUMZLT-UHFFFAOYSA-N Fluorine atom Chemical compound [F] YCKRFDGAMUMZLT-UHFFFAOYSA-N 0.000 claims description 5
- 229910052731 fluorine Inorganic materials 0.000 claims description 5
- 239000011737 fluorine Substances 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 claims description 5
- 230000005669 field effect Effects 0.000 claims description 3
- 230000000873 masking effect Effects 0.000 claims 1
- 238000003825 pressing Methods 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 description 7
- 238000004382 potting Methods 0.000 description 6
- 239000000758 substrate Substances 0.000 description 5
- 239000008280 blood Substances 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 229910052594 sapphire Inorganic materials 0.000 description 3
- 239000010980 sapphire Substances 0.000 description 3
- 150000003672 ureas Chemical class 0.000 description 3
- 239000004593 Epoxy Substances 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000004375 Dextrin Substances 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 229920000084 Gum arabic Polymers 0.000 description 1
- 239000004642 Polyimide Substances 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- 241000978776 Senegalia senegal Species 0.000 description 1
- 235000010489 acacia gum Nutrition 0.000 description 1
- 239000000205 acacia gum Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 229920006332 epoxy adhesive Polymers 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229920001721 polyimide Polymers 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
Landscapes
- Examining Or Testing Airtightness (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は血液等の中に含まれる尿素を検出する
尿素センサの製造方法に関するものである。DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a method of manufacturing a urea sensor for detecting urea contained in blood or the like.
従来、尿素センサチツプは、第２図に示すよう
に、サフアイア基板１の片面にイオン感応性電界
効果型トランジスタ２を設け、このトランジスタ
２の表面に酵素の固定化膜３，４を設けており、
この固定化膜３，４の近傍がセンサ部５である。
Conventionally, a urea sensor chip, as shown in FIG. 2, has an ion-sensitive field effect transistor 2 provided on one side of a sapphire substrate 1, and enzyme immobilization films 3 and 4 provided on the surface of this transistor 2.
The sensor section 5 is located near the immobilized films 3 and 4.
この尿素センサは、第３図、第４図に示すよう
に、センサチツプ６をプリント基板７に接着剤９
によつて固着し、このセンサチツプ６とプリント
基板７との上の所望の配線パターン８，８′間を
ワイヤ１１のワイヤボンデイングによつて接続
し、この周囲をポツテイング剤１２によつて覆つ
ている。 As shown in FIGS. 3 and 4, this urea sensor is constructed by attaching a sensor chip 6 to a printed circuit board 7 using an adhesive 9.
The desired wiring patterns 8 and 8' on the sensor chip 6 and the printed circuit board 7 are connected by wire bonding with a wire 11, and the periphery thereof is covered with a potting agent 12. .
この尿素センサの使用に際しては、センサチツ
プを血液中に浸漬するか、またはセンサチツプに
数滴の血液を滴下し、その時の酵素の反応を失活
酵素との電位差によつて表わし、血液中の尿素を
検出するものである。なお、測定を行なつた使用
済みのセンサは消耗品として破棄することになる
ため、非常に安価なものを供給する必要がある。
また、センサチツプが正常に反応するためには酵
素が安定でなければならないが、この酵素を安定
に保つにはセンサチツプの部分を水中に浸漬する
のが最良である。この尿素センサを入手後に直ち
に使用するものであれば問題ないが、１〜２年間
もストツクする場合は水中に浸漬しておく必要が
ある。したがつて、使用接着剤やポツテイング剤
は長期間に亘つて耐水性が必要となる。 When using this urea sensor, the sensor chip is immersed in blood, or several drops of blood are dropped onto the sensor chip, and the reaction of the enzyme at that time is expressed by the potential difference with the inactivated enzyme, and the urea in the blood is measured. It is something to detect. Incidentally, since the used sensor that has been used for measurement will be discarded as a consumable item, it is necessary to supply a very inexpensive sensor.
Furthermore, in order for the sensor chip to react normally, the enzyme must be stable, and the best way to keep this enzyme stable is to immerse the sensor chip in water. There is no problem if this urea sensor is used immediately after obtaining it, but if it is to be stored for 1 to 2 years, it must be immersed in water. Therefore, the adhesives and potting agents used need to be water resistant for a long period of time.
接着剤については数多く選定し加速試験の結
果、耐水性の極めて良好なものを入手することが
できた。 After selecting many adhesives and conducting accelerated tests, we were able to obtain one with extremely good water resistance.
しかしながら、ポツテイング剤の場合長期に亘
つて耐水性の極めて良好なものは殆んどない。
However, there are almost no potting agents that have extremely good water resistance over a long period of time.
本発明の目的はこの様な欠点を無くすために、
従来のポツテイング剤の代りに前記の耐水性の極
めて良好な接着剤でセンサチツプのセンサ部以外
の部分およびヤイヤ近傍をふつ素系ゴム棒を用い
て被覆するものであり、品質が良く、信頼性を向
上させた尿素センサを提供することにある。 The purpose of the present invention is to eliminate such drawbacks,
Instead of the conventional potting agent, the above-mentioned highly water-resistant adhesive is used to cover the parts of the sensor chip other than the sensor part and the vicinity of the wire using a fluorine-based rubber rod, resulting in high quality and reliability. An object of the present invention is to provide an improved urea sensor.
イオン感応性電界効果トランジスタの表面に酵
素の固定化膜を設けたセンサチツプをプリント基
板に実装した尿素センサの製造方法において、前
記チツプと前記プリント基板を位置決め後、前記
センサ部をふつ素系ゴム棒で抑え付けてマスキン
グをした後に、全体を耐水性の接着剤で覆い、前
記接着剤が固化する前、もしくは固化後にゴム棒
を取外した事を特徴とする。
In a method for manufacturing a urea sensor in which a sensor chip having an enzyme immobilized film provided on the surface of an ion-sensitive field effect transistor is mounted on a printed circuit board, after positioning the chip and the printed circuit board, the sensor part is attached to a fluorine-based rubber rod. After being pressed down and masked, the entire body is covered with a water-resistant adhesive, and the rubber rod is removed before or after the adhesive hardens.
第１図ａ，ｂ，ｃは本発明方法の一実施例を説
明するための工程を示した実装断面図、第２図は
第１図のサフアイア基板で、その上にトランジス
タ２を形成し、その上に失活した酵素固定化膜３
と酵素固定膜４とを形成し、それらをセンサ部５
とし、全体でセンサチツプ６を構成している。
1a, b, and c are mounting cross-sectional views showing steps for explaining an embodiment of the method of the present invention, and FIG. 2 is the sapphire substrate of FIG. 1, on which a transistor 2 is formed, Deactivated enzyme-immobilized membrane 3 on top of it
and an enzyme-immobilized membrane 4, and connect them to the sensor section 5.
The sensor chip 6 is constructed as a whole.
第１図ａにおいて、７はプリント基板であり、
一端にセンサチツプ５との配線パターン８、他端
に計測器と接続するためのパターン８′を有して
いる。なお、このプリント基板７についてはフレ
キシブルなものでも良好であり、また材質につい
てもポリイミド、エポキシ等いづれでも良い。ま
た、９は接着剤であり、耐水性についてはエポキ
シ系の耐水性用接着剤〔例えば「クイツク30」，
「クイツク５」（小西六(株)製）〕が極めて良好であ
る。他の接着剤としてはニカワ、デキストリン、
アラビアゴム、ポリビニールアルコール、ポリ酢
酸エマルジヨンがある。また量産性を考慮すると
デイスペンサを利用することが必要であり、その
ためには粘度の高いものが良好である。なお、セ
ンサチツプ６とパターン８間はワイヤボンデイン
グが出来易いように位置決めを行ない、基板７に
接着剤９によつて強固に固着する。１０はワイヤ
でありボンデイングした状態を示しており、セン
サチツプ６とパターン８の所望間で接続されてい
る。 In FIG. 1a, 7 is a printed circuit board,
It has a wiring pattern 8 for connecting to the sensor chip 5 at one end, and a pattern 8' for connecting to a measuring device at the other end. The printed circuit board 7 may be flexible, and may be made of polyimide, epoxy, or the like. In addition, 9 is an adhesive, and for water resistance, an epoxy-based water-resistant adhesive [for example, "Quick 30",
``Quitsuku 5'' (manufactured by Konishiroku Co., Ltd.) is extremely good. Other adhesives include glue, dextrin,
There are gum arabic, polyvinyl alcohol, and polyacetic acid emulsion. Further, considering mass production, it is necessary to use a dispenser, and for that purpose, a dispenser with high viscosity is preferable. The sensor chip 6 and the pattern 8 are positioned so as to facilitate wire bonding, and are firmly fixed to the substrate 7 with an adhesive 9. A wire 10 is shown in a bonded state, and is connected between the sensor chip 6 and the pattern 8 at desired points.
このワイヤ１０のワイヤボンデイングに際して
は、加熱型のAuワイヤであると、熱によつて酵
素に悪影響を及ぼすため、常温型のAlワイヤ超
音波ボンデイングの方が良好である。また、１３
は接着剤であり、接着剤９と全く同じものであ
る。すなわち、センサチツプ６を基板７に固着
後、にワイヤ１０の保護を行うと共にワイヤ１０
間のリークを防止することを目的としている。こ
れは従来のボツテイング剤１１の代りに用いてい
るものである。接着剤１３の盛上げに際してはセ
ンサ部５の位置にフツソ系ゴム棒１２を押し付け
た状態で全体的に覆う。ゴム棒１２の引き抜きに
際しては接着剤が固化後に行うと第１図ｂの様に
なり、接着剤が固化前に引き抜きを行うと第１図
ｃのように少し流れ出すが、いずれにあいても使
用上問題ない。また、ゴム棒１２の押し付に際し
てはゴム系であるためによく密着が行われ、接着
剤１３が浸透することはない。また、ゴム棒１２
の引き抜きに際してはこのゴム棒１２がふつ素系
であるため接着されずに簡単に抜くことができ
る。なお、この接着剤１３については耐水性の良
好なものは存在するが、ポツテイング剤と比較し
て問題になるのは接着剤固化時の収縮率である。
接着剤固化時に収縮率が大きいとワイヤを切断す
るおそれがある。しかし、我々が選定した耐水性
エポキシ接着剤は体績収率が殆んどなく、これを
利用すると同時にポツトライフの長いものを利用
したものであり、実験によればワイヤの切断は生
じなかつた。 When wire bonding the wire 10, if a heated Au wire is used, the heat will have a negative effect on the enzyme, so room temperature Al wire ultrasonic bonding is better. Also, 13
is an adhesive and is exactly the same as adhesive 9. That is, after fixing the sensor chip 6 to the substrate 7, the wire 10 is protected and the wire 10 is
The purpose is to prevent leaks between This is used in place of the conventional botting agent 11. When applying the adhesive 13, the sensor portion 5 is entirely covered with the fused rubber rod 12 pressed against it. If the rubber rod 12 is pulled out after the adhesive has solidified, the result will be as shown in Figure 1b, and if it is pulled out before the adhesive has solidified, it will flow out a little as shown in Figure 1c, but in either case it will not be used. There is no problem. Furthermore, when the rubber rod 12 is pressed, since it is made of rubber, good adhesion is achieved and the adhesive 13 does not penetrate. In addition, the rubber rod 12
Since the rubber rod 12 is made of fluorine, it can be easily removed without being bonded. Although there are adhesives 13 that have good water resistance, what poses a problem compared to potting agents is the shrinkage rate when the adhesive hardens.
If the shrinkage rate is large when the adhesive hardens, there is a risk of cutting the wire. However, the water-resistant epoxy adhesive we selected had almost no yield, and at the same time we used a material with a long pot life, and experiments showed that no wire breakage occurred.
以上説明したように、本考案によれば、耐水性
接着剤が完全に水をシヤツトアウトするため、長
期間に亘つて尿素センサを水中に浸漬しても酵素
を失活させることなく、センサとしての機能を満
足させることができ、信頼性が極めて高くなる。
もちろんチツプの接着とワイヤの被覆は別工程で
あるが同じ接着剤、同じデイスペンサを用いるこ
とができるなど、極めて有利であり、且つふつ素
系ゴム棒１２を利用することによつて接着剤によ
る全体への被覆がセンサ部を気にせず行える等に
よつて極めて良好なセンサを提供することができ
る。
As explained above, according to the present invention, the water-resistant adhesive completely shuts out water, so even if the urea sensor is immersed in water for a long period of time, the enzyme will not be deactivated, and the sensor will remain functional. Functionality can be satisfied and reliability is extremely high.
Of course, adhesion of the chip and coating of the wire are separate processes, but it is extremely advantageous that the same adhesive and the same dispenser can be used. An extremely good sensor can be provided because the coating can be done without worrying about the sensor part.
第１図は本考案の一実施例を示す実装断面図
で、ａ，ｂ，ｃはその工程図、第２図は第１図の
チツプの一例の断面図、第３図は従来のセンサチ
ツプとプリント基板との実装断面図、第４図は全
体の構成を示す斜視図である。
１……サフアイア基板、２……トランジスタ、
３……失活した酵素固定化膜、４…酵素固定化
膜、５……センサ部、６……センサチツプ、７…
…プリント基板、８，８′……配線パターン、９
……接着剤、１０……ワイヤ、１１……ポツテイ
ング剤、１２……フツ素系ゴム棒、１３……接着
剤。
Fig. 1 is a cross-sectional view of an implementation of an embodiment of the present invention, a, b, and c are its process diagrams, Fig. 2 is a cross-sectional view of an example of the chip shown in Fig. 1, and Fig. 3 is a cross-sectional view of a conventional sensor chip. FIG. 4 is a cross-sectional view of the mounting on a printed circuit board, and a perspective view showing the overall configuration. 1...Sapphire substrate, 2...Transistor,
3... Deactivated enzyme-immobilized membrane, 4... Enzyme-immobilized membrane, 5... Sensor part, 6... Sensor chip, 7...
...Printed circuit board, 8, 8'...Wiring pattern, 9
... Adhesive, 10 ... Wire, 11 ... Potting agent, 12 ... Fluorine rubber rod, 13 ... Adhesive.
Claims (1)
に酵素の固定化膜を設けたセンサチツプをプリン
ト基板に実装した尿素センサの製造方法におい
て、前記チツプと前記プリント基板を位置決め
後、前記センサ部の上部をふつ素系ゴム棒で前記
プリント基板に抑え付けてマスキングをした後
に、全体を耐水性の接着剤で覆い、前記接着剤が
固化する前、もしくは固化後に前記ふつ素系ゴム
棒を取外した事を特徴とする尿素センサの製造方
法。1. In a method for manufacturing a urea sensor in which a sensor chip having an enzyme immobilized film provided on the surface of an ion-sensitive field effect transistor is mounted on a printed circuit board, after positioning the chip and the printed circuit board, the upper part of the sensor section is opened. A feature is that after masking the printed circuit board by pressing it with a bare rubber rod, the entire surface is covered with a water-resistant adhesive, and the fluorine rubber rod is removed before or after the adhesive hardens. A method for manufacturing a urea sensor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61163009A JPS6318260A (en) | 1986-07-10 | 1986-07-10 | Manufacture of urea sensor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61163009A JPS6318260A (en) | 1986-07-10 | 1986-07-10 | Manufacture of urea sensor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6318260A JPS6318260A (en) | 1988-01-26 |
| JPH0481741B2 true JPH0481741B2 (en) | 1992-12-24 |
Family
ID=15765459
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61163009A Granted JPS6318260A (en) | 1986-07-10 | 1986-07-10 | Manufacture of urea sensor |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6318260A (en) |
-
1986
- 1986-07-10 JP JP61163009A patent/JPS6318260A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6318260A (en) | 1988-01-26 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |