JPH0519656B2 - - Google Patents
Info
- Publication number
- JPH0519656B2 JPH0519656B2 JP59107163A JP10716384A JPH0519656B2 JP H0519656 B2 JPH0519656 B2 JP H0519656B2 JP 59107163 A JP59107163 A JP 59107163A JP 10716384 A JP10716384 A JP 10716384A JP H0519656 B2 JPH0519656 B2 JP H0519656B2
- Authority
- JP
- Japan
- Prior art keywords
- electrophoresis
- voltage
- electrode
- tube
- detector
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000001962 electrophoresis Methods 0.000 claims description 42
- 239000003792 electrolyte Substances 0.000 claims description 25
- 230000005012 migration Effects 0.000 claims description 16
- 238000013508 migration Methods 0.000 claims description 16
- 230000003189 isokinetic effect Effects 0.000 claims description 10
- 238000002347 injection Methods 0.000 claims description 5
- 239000007924 injection Substances 0.000 claims description 5
- 238000002218 isotachophoresis Methods 0.000 description 6
- 238000010586 diagram Methods 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000009413 insulation Methods 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000004452 microanalysis Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 239000013076 target substance Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44713—Particularly adapted electric power supply
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Power Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Electrochromic Elements, Electrophoresis, Or Variable Reflection Or Absorption Elements (AREA)
- Peptides Or Proteins (AREA)
Description
【発明の詳細な説明】
(イ) 産業上の利用分野
この発明は、等速電気泳動装置に関する。さら
に詳しくは、電気泳動中の両極間の泳動電圧を改
善させた等速電気泳動装置に関する。DETAILED DESCRIPTION OF THE INVENTION (a) Field of Industrial Application This invention relates to an isokinetic electrophoresis device. More specifically, the present invention relates to an isotachophoresis device that improves the electrophoresis voltage between the two electrodes during electrophoresis.
(ロ) 従来技術
等速電気泳動分析法は、泳動管内部にターミナ
ル電解液とリーデイング電解液とを充填し、この
両電解液の境界面に荷電しうる物質、例えばアミ
ノ酸類、ペプチド類、生体物質等の試料を注入し
て定電流下電気泳動を行ない、易動度の差によつ
て被検出物を単一ゾーンに分離し適宜検出器にて
定性や定量を行なう方法であり、上述のような試
料の微量分析に好適な方法である。(b) Prior art In the isotachophoresis analysis method, a terminal electrolyte and a leading electrolyte are filled inside a migration tube, and a substance that can be charged, such as amino acids, peptides, and living organisms, is added to the interface between the two electrolytes. This is a method in which a sample such as a substance is injected and subjected to constant current electrophoresis, and the target substance is separated into a single zone based on the difference in mobility, and qualitative and quantitative analysis is performed using a detector as appropriate. This method is suitable for microanalysis of such samples.
しかしながら、従来の等速電気泳動装置におい
てかような分析を意図して電気泳動を行なうと、
導電率の小さいターミナル電解液のゾーンが泳動
と共に両極間に増加するため両極間電圧がしばし
ば高い値（例えば20KV）を示す。そのため、泳
動用電源の容量を大きくしなければならず、さら
に、ベース電圧が高くなるので目的の試料のゾー
ンの分離検知が困難となりしかも系全体の絶縁性
を向上させる必要がある等の多くの問題点があつ
た。 However, when electrophoresis is performed with the intention of such analysis using a conventional isotachophoresis device,
The voltage between the electrodes often exhibits a high value (for example, 20 KV) because a zone of terminal electrolyte with low conductivity increases with migration between the electrodes. Therefore, the capacity of the power supply for electrophoresis must be increased, and the base voltage becomes high, making it difficult to separate and detect the target sample zone.In addition, there are many problems such as the need to improve the insulation of the entire system. There was a problem.
(ハ) 目的
この発明は、かような状況に鑑みなされたもの
であり、泳動電圧を低下しうる等速電気泳動装置
を提供しようとするものである。(c) Purpose This invention was made in view of the above situation, and aims to provide an isokinetic electrophoresis device that can reduce the electrophoresis voltage.
(ニ) 構成
かくしてこの発明によれば、ターミナル電解液
槽とリーデイング電解液槽とを泳動管で接続する
と共にこの泳動管に試料注入口及び電位勾配検出
器を設けてなる等速電気泳動装置において、
ターミナル電解液槽内に設定された電極を電位
勾配検出器に近接させうる電極移動手段又は泳動
管移動手段と、電気泳動中の泳動電圧をモニター
する電圧検知器を備え、泳動電圧が所定の値を越
えた時に上記移動手段を動作させて泳動電圧を上
昇防止又は低下を行ないうるよう構成してなる等
速電気泳動装置が提供される。(D) Configuration According to the present invention, in an isokinetic electrophoresis apparatus in which a terminal electrolyte tank and a leading electrolyte tank are connected by a migration tube, and the migration tube is provided with a sample injection port and a potential gradient detector. , Equipped with electrode moving means or electrophoresis tube moving means that can bring the electrode set in the terminal electrolyte tank close to the potential gradient detector, and a voltage detector that monitors the electrophoresis voltage during electrophoresis, and is equipped with a voltage detector that monitors the electrophoresis voltage during electrophoresis. Provided is an isokinetic electrophoresis apparatus which is configured to operate the moving means to prevent or reduce the electrophoresis voltage when the electrophoresis voltage exceeds this value.
上記泳動電圧の所定値とは、少なくとも電極又
は泳動管移動手段を動作させないで電気泳動分析
を行ないうる泳動電圧より低い電圧値を意味し、
電位勾配検出器の性能、電源の容量等が許容しう
る電圧内でできるだけ低い値を設定するのが適当
である。通常、電極や泳動管の移動距離を調整す
ることにより従来装置で要する最大極間電圧の70
〜80％をカツトした値に設定することが可能であ
る。 The predetermined value of the electrophoresis voltage means a voltage value lower than the electrophoresis voltage at which electrophoresis analysis can be performed without operating at least the electrode or the electrophoresis tube moving means,
It is appropriate to set the value as low as possible within the range of voltage that the performance of the potential gradient detector, the capacity of the power supply, etc. can allow. Normally, by adjusting the moving distance of the electrodes and migration tube, the maximum electrode-to-electrode voltage required by conventional equipment can be reduced by 70%.
It is possible to set the value to a value that cuts ~80%.
(ホ) 実施例
以下、この発明の等速電気泳動装置を実施例に
より詳説する。(e) Examples Hereinafter, the isotachophoresis apparatus of the present invention will be explained in detail with reference to examples.
第１図に示す１Ａは、この発明の等速電気泳動
装置の一実施例を示す構成説明図である。図にお
いて電気泳動装置１Ａは、ターミナル電解液槽２
とリーデイング電解液槽３とを泳動管４で接続し
てなり、泳動管のターミナル電解液槽側には試料
注入口４１が、リーデイング電解液槽側には電位
勾配検出器４２が設けられている。そして、ター
ミナル電解液槽２及びリーデイング電解液槽３内
にはそれぞれ電極２１，３１が装着されておりこ
れらは定電流直流電源５に接続されて電気泳動電
圧を印加できるように設定されているが、ターミ
ナル電解液槽２内の電極２１は、液密なパツキン
２３を介して泳動管４の軸方向に水平移動可能と
なつており、ラツクピニオン機構とステツピング
モーターを備えた電極移動部２２によつて支持さ
れている。また、５１は両極間の電圧をモニター
する電圧検知器を備えた制御部であり、この検知
電圧が所定値（例えば4KV）を越えた時に電極
移動部２２に信号を送り電極２１を電位勾配検出
器の方向に徐々に移動させて検知電圧が所定値を
越えないようにフイードバツク制御しうるプログ
ラムが内在されている。 1A shown in FIG. 1 is a configuration explanatory diagram showing an embodiment of the isokinetic electrophoresis apparatus of the present invention. In the figure, the electrophoresis device 1A is a terminal electrolyte tank 2.
and a leading electrolyte tank 3 are connected by a migration tube 4, and a sample injection port 41 is provided on the terminal electrolyte tank side of the migration tube, and a potential gradient detector 42 is provided on the leading electrolyte tank side. . Electrodes 21 and 31 are installed in the terminal electrolyte tank 2 and the leading electrolyte tank 3, respectively, and these are connected to a constant current DC power source 5 and set so that an electrophoresis voltage can be applied. The electrode 21 in the terminal electrolyte tank 2 can be moved horizontally in the axial direction of the migration tube 4 via a liquid-tight packing 23, and is connected to an electrode moving section 22 equipped with a rack and pinion mechanism and a stepping motor. It has been well supported. Further, 51 is a control unit equipped with a voltage detector that monitors the voltage between the two electrodes, and when this detected voltage exceeds a predetermined value (for example, 4KV), it sends a signal to the electrode moving unit 22 and detects the potential gradient of the electrode 21. There is a built-in program that can perform feedback control so that the detection voltage does not exceed a predetermined value by gradually moving it in the direction of the sensor.
かかる等速電気泳動装置の動作について以下説
明する。 The operation of such an isotachophoresis apparatus will be explained below.
まず、第４図は等速電気泳動における泳動管内
部のゾーンの状態の経時変化を示すものであり、
Ａは電気泳動開示時でありＴはターミナル電解
液、Ｓは注入試料、Ｌはリーデイング電解液を意
味する。電気泳動が進むにつれてゾーンはＢ，Ｃ
の状態を経てＤの状態となり例えば試料中の成分
SA，SBが分離され等速ゾーンとなつて電位勾配
検出器４２に移送されることとなる。この際（図
中、Ｄ）の電位曲線を第５図に示した。このよう
に、電位勾配はターミナル電解液が最も大（P0
→P2）でリーデイング電解液が最も小（P4→Ｐ）
であり、その間にSBとSAの勾配（P2→P3）（P3→
P4）が存在しているが、泳動が進むにつれてＡ
→Ｄのようにターミナル電解液Ｔが検出器４２の
方に移動するため結果的に泳動電圧は高いものと
なる（図中Ｐ→P0）。 First, Figure 4 shows the change in the state of the zone inside the electrophoresis tube over time during isokinetic electrophoresis.
A is the start of electrophoresis, T is the terminal electrolyte, S is the injection sample, and L is the leading electrolyte. As electrophoresis progresses, zones B and C
After passing through the state, it becomes state D, for example, the components in the sample
S A and S B are separated, become a constant velocity zone, and are transferred to the potential gradient detector 42 . The potential curve at this time (D in the figure) is shown in FIG. Thus, the potential gradient is largest in the terminal electrolyte (P 0
→P 2 ), the leading electrolyte is the smallest (P 4 →P)
, and between them the gradients of S B and S A (P 2 → P 3 ) (P 3 →
P 4 ), but as the migration progresses, A
As the terminal electrolyte T moves toward the detector 42 as shown in →D, the migration voltage becomes high as a result (P→P 0 in the figure).
しかしながら、前記実施例の装置においては、
第４図Ｅのごとくターミナル電解液の移動と共に
ある時点（例えば、電圧が4KVを越えた時点）
から電極自体が検出器４２方向に移動（第４図、
Ｅ参照）するため第５図におけるP1−P0間の電
圧がカツトされ、泳動電圧はＰ−P1となり無駄
な電圧消費を避けることができる。そして、成分
SA，SBの電位勾配についても不都合は生じない。 However, in the device of the above embodiment,
As shown in Figure 4E, there is a certain point when the terminal electrolyte moves (for example, when the voltage exceeds 4KV)
The electrode itself moves toward the detector 42 (Fig. 4,
(see E), the voltage between P 1 and P 0 in FIG. 5 is cut off, and the electrophoresis voltage becomes P-P 1 , thereby avoiding wasteful voltage consumption. And the ingredients
There is no problem with the potential gradients of S A and SB .
なお、前記実施例においては、電極を移動させ
る手段を備えたものについて説明したが、基本的
に電極と電位勾配検出器に近接させうる構成であ
ればよく、例えば、第２図に示すごとく泳動管４
とターミナル電解液槽２との接続部を液密状態で
摺動しうる二重管４３構造としかつ泳動管４を電
極２１に近接させうる泳動管移動ブロツク２４を
設けることにより、泳動管移動部２２′によつて
前記と同様に泳動電圧を制御しうるよう構成して
もよい。また、第３図に示すごとく、ターミナル
電極槽２における電極を主電極２１ａと補助電極
２１ｂから構成し、補助電極２１ｂを第１図の電
極２１と同様に移動可能に構成しかつ電源５の一
部の電圧をここへ印加しうるよう構成することも
できる。なお５１′は電圧供給部も含む制御部で
ある。 In addition, in the above embodiment, an explanation has been given of a device equipped with a means for moving the electrode, but basically any configuration that allows the electrode to be brought close to the potential gradient detector is sufficient. For example, as shown in FIG. tube 4
The connection between the electrophoresis tube 4 and the terminal electrolyte tank 2 has a double tube 43 structure that can slide in a liquid-tight state, and the migration tube movement block 24 that can bring the migration tube 4 close to the electrode 21 is provided. 22' may be configured to control the migration voltage in the same manner as described above. Further, as shown in FIG. 3, the electrodes in the terminal electrode tank 2 are composed of a main electrode 21a and an auxiliary electrode 21b, and the auxiliary electrode 21b is configured to be movable like the electrode 21 in FIG. It is also possible to apply a voltage to this part. Note that 51' is a control section that also includes a voltage supply section.
(ヘ) 効果
以上述べたごとくこの発明の等速電気泳動装置
は、高電圧が必要とされていた泳動電圧を低下さ
せることができる。従つて、泳動用電源の容量こ
とに最大電圧を低くすることができる、試料のア
イソレーシヨン技術が簡単となる、系の絶縁性を
向上させる対策が特に不要である、取り扱い上の
安全性を向上できる、等の種々の利点を備え有用
なものである。(F) Effects As described above, the isotachophoresis apparatus of the present invention can reduce the electrophoresis voltage, which previously required a high voltage. Therefore, the capacity and maximum voltage of the power source for electrophoresis can be lowered, the isolation technique for the sample is simplified, there is no need to take special measures to improve the insulation of the system, and handling safety is improved. It is useful because it has various advantages such as improved performance.
第１図は、この発明の等速電気泳動装置の一実
施例を示す構成説明図、第２図及び第３図は同じ
く他の実施例をそれぞれ示す構成説明図、第４図
Ａ〜Ｅはこの発明の電気泳動分析装置による使用
状態を説明するための模式図、第５図は同じく使
用時の各ゾーンと電位との関係を説明するための
グラフ図である。
１Ａ，１Ｂ，１Ｃ……等速電気泳動装置、２…
…ターミナル電解液槽、３……リーデイング電解
液槽、４……泳動管、５……定電流直流電源、２
１，３１……電極、２１ａ……主電極、２１ｂ…
…補助電極、２２……電極移動部、２２′……泳
動管移動部、２３……パツキン、２４……移動ブ
ロツク、４１……試料注入口、４２……電位勾配
検出器、４３……二重管、５１，５１′……制御
部。
FIG. 1 is a structural explanatory diagram showing one embodiment of the isokinetic electrophoresis apparatus of the present invention, FIGS. 2 and 3 are structural explanatory diagrams showing other embodiments, respectively, and FIGS. 4 A to E are FIG. 5 is a schematic diagram for explaining the state of use of the electrophoretic analyzer of the present invention, and a graph diagram for explaining the relationship between each zone and the potential during use. 1A, 1B, 1C...Isokinetic electrophoresis device, 2...
...Terminal electrolyte tank, 3...Leading electrolyte tank, 4...Migration tube, 5...Constant current DC power supply, 2
1, 31...electrode, 21a...main electrode, 21b...
...Auxiliary electrode, 22... Electrode moving section, 22'... Electrophoresis tube moving section, 23... Packing, 24... Moving block, 41... Sample injection port, 42... Potential gradient detector, 43... Two Heavy pipe, 51, 51'... control section.
Claims (1)
とを泳動管で接続すると共にこの泳動管に試料注
入口及び電位勾配検出器を設けてなる等速電気泳
動装置において、 ターミナル電解液槽内に設定された電極を電位
勾配検出器に近接させうる電極移動手段又は泳動
管移動手段と、電気泳動中の泳動電圧をモニター
する電圧検出器を備え、泳動電圧が所定の値を越
えた時に上記移動手段を動作させて泳動電圧の上
昇防止又は低下を行ないうるよう構成してなる等
速電気泳動装置。[Scope of Claims] 1. In an isokinetic electrophoresis apparatus in which a terminal electrolyte tank and a leading electrolyte tank are connected by a migration tube, and the migration tube is provided with a sample injection port and a potential gradient detector, Equipped with an electrode moving means or an electrophoresis tube moving means that allows the electrode set in the tank to be brought close to the potential gradient detector, and a voltage detector that monitors the electrophoresis voltage during electrophoresis, and when the electrophoresis voltage exceeds a predetermined value. An isokinetic electrophoresis device configured such that the moving means can be operated at times to prevent an increase or decrease in electrophoresis voltage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59107163A JPS60250243A (en) | 1984-05-25 | 1984-05-25 | Isokinetic electrophoresis device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59107163A JPS60250243A (en) | 1984-05-25 | 1984-05-25 | Isokinetic electrophoresis device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS60250243A JPS60250243A (en) | 1985-12-10 |
| JPH0519656B2 true JPH0519656B2 (en) | 1993-03-17 |
Family
ID=14452083
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP59107163A Granted JPS60250243A (en) | 1984-05-25 | 1984-05-25 | Isokinetic electrophoresis device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS60250243A (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5950429B2 (en) * | 2011-05-13 | 2016-07-13 | シャープ株式会社 | Electrophoresis method and electrophoresis apparatus |
-
1984
- 1984-05-25 JP JP59107163A patent/JPS60250243A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS60250243A (en) | 1985-12-10 |
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