JPH0524455B2 - - Google Patents
Info
- Publication number
- JPH0524455B2 JPH0524455B2 JP58250168A JP25016883A JPH0524455B2 JP H0524455 B2 JPH0524455 B2 JP H0524455B2 JP 58250168 A JP58250168 A JP 58250168A JP 25016883 A JP25016883 A JP 25016883A JP H0524455 B2 JPH0524455 B2 JP H0524455B2
- Authority
- JP
- Japan
- Prior art keywords
- terminal
- liquid
- leading
- terminal liquid
- analysis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000007788 liquid Substances 0.000 claims description 61
- 238000004458 analytical method Methods 0.000 claims description 23
- 150000002500 ions Chemical class 0.000 claims description 18
- 238000001962 electrophoresis Methods 0.000 claims description 10
- 238000002218 isotachophoresis Methods 0.000 claims description 9
- 239000000243 solution Substances 0.000 claims description 9
- 239000008151 electrolyte solution Substances 0.000 claims description 5
- 230000003189 isokinetic effect Effects 0.000 claims description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 6
- SXGZJKUKBWWHRA-UHFFFAOYSA-N 2-(N-morpholiniumyl)ethanesulfonate Chemical compound [O-]S(=O)(=O)CC[NH+]1CCOCC1 SXGZJKUKBWWHRA-UHFFFAOYSA-N 0.000 description 5
- 239000003792 electrolyte Substances 0.000 description 5
- 150000007524 organic acids Chemical class 0.000 description 5
- UCMIRNVEIXFBKS-UHFFFAOYSA-N beta-alanine Chemical compound NCCC(O)=O UCMIRNVEIXFBKS-UHFFFAOYSA-N 0.000 description 4
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 4
- 230000005012 migration Effects 0.000 description 4
- 238000013508 migration Methods 0.000 description 4
- JXKPEJDQGNYQSM-UHFFFAOYSA-M sodium propionate Chemical compound [Na+].CCC([O-])=O JXKPEJDQGNYQSM-UHFFFAOYSA-M 0.000 description 4
- 239000004324 sodium propionate Substances 0.000 description 4
- 235000010334 sodium propionate Nutrition 0.000 description 4
- 229960003212 sodium propionate Drugs 0.000 description 4
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 3
- 239000000460 chlorine Substances 0.000 description 3
- 229910052801 chlorine Inorganic materials 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 235000014113 dietary fatty acids Nutrition 0.000 description 3
- 229930195729 fatty acid Natural products 0.000 description 3
- 239000000194 fatty acid Substances 0.000 description 3
- 150000004665 fatty acids Chemical class 0.000 description 3
- 235000005985 organic acids Nutrition 0.000 description 3
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 2
- 229920004890 Triton X-100 Polymers 0.000 description 2
- 239000013504 Triton X-100 Substances 0.000 description 2
- 235000011054 acetic acid Nutrition 0.000 description 2
- 229940000635 beta-alanine Drugs 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- FUZZWVXGSFPDMH-UHFFFAOYSA-N hexanoic acid Chemical compound CCCCCC(O)=O FUZZWVXGSFPDMH-UHFFFAOYSA-N 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- -1 polyoxyethylene Polymers 0.000 description 2
- 235000019260 propionic acid Nutrition 0.000 description 2
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- NQPDZGIKBAWPEJ-UHFFFAOYSA-N valeric acid Chemical compound CCCCC(O)=O NQPDZGIKBAWPEJ-UHFFFAOYSA-N 0.000 description 2
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 1
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 description 1
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 1
- OFOBLEOULBTSOW-UHFFFAOYSA-N Propanedioic acid Natural products OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-N Succinic acid Natural products OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 1
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 description 1
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 1
- 150000001450 anions Chemical group 0.000 description 1
- KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 description 1
- 235000015165 citric acid Nutrition 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
- 230000020169 heat generation Effects 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 1
- 239000011976 maleic acid Substances 0.000 description 1
- 239000001630 malic acid Substances 0.000 description 1
- 235000011090 malic acid Nutrition 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000010287 polarization Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000011975 tartaric acid Substances 0.000 description 1
- 235000002906 tartaric acid Nutrition 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
- 229940005605 valeric acid Drugs 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44747—Composition of gel or of carrier mixture
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Dispersion Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Description
(イ) 産業上の利用分野
この発明は等速電気泳動分析法に関し、特にタ
ーミナル液の利用技術の改良に関する。
(ロ) 従来技術
等速電気泳動分析法は、泳動細管内のリーデイ
ング液とターミナル液との間に試料成分を注入
し、定電流下で泳動させると、試料成分が、それ
ぞれの成分に対応する移動度の順にならんで分離
されるることを利用する分析法であるが、この等
速電気泳動分析法におけるリーデイング液とター
ミナル液との組み合せに最適条件である。例え
ば、試料成分が陰イオンの場合の分析では、リー
デイング液とターミナル液との最適の組み合せと
して第１表のものがよく用いられている。この表
において、リーデイング液のPHが3.6のとき、タ
ーミナル液にモルホリノエタンスルホン酸
（MES）を使用すると、ターミナル液の移動度が
小さくなり、通電性が悪く、分析操作上好ましく
ない。このため、リーデイング液のPHを変更する
か、ターミナル液を変更する必要がある。
(a) Field of Industrial Application This invention relates to isotachophoretic analysis, and in particular to improvements in techniques for utilizing terminal liquid. (b) Prior art In the isotachophoresis analysis method, sample components are injected between the leading liquid and the terminal liquid in the electrophoresis tube, and when the sample components are electrophoresed under a constant current, the sample components correspond to each other. This is an analysis method that utilizes separation in the order of mobility, and this is the optimal condition for the combination of a leading liquid and a terminal liquid in this isotachophoresis analysis method. For example, in the analysis when the sample component is an anion, the optimal combination of leading liquid and terminal liquid shown in Table 1 is often used. In this table, when the PH of the leading liquid is 3.6, if morpholinoethanesulfonic acid (MES) is used as the terminal liquid, the mobility of the terminal liquid will be low and the conductivity will be poor, which is not preferable for analytical operations. Therefore, it is necessary to change the PH of the leading liquid or change the terminal liquid.
【表】【table】
【表】
しかしこのような分析条件の変更は分析操作上
煩雑である。
(ハ) 目的
この発明はこれらの事情に鑑みなされたもの
で、その主要な目的の一つは、リーデイング液の
PHや組成が変つても、もとのターミナル液を変え
ないで、操作性よく両液の最適条件で分析できる
方法を提供することにある。
(ニ) 構成
この発明は等速電気泳動分析法において、移動
度がリーデイングイオンとターミナルイオンの間
で、しかも試料成分よりも低い移動度のイオンを
含む電解液を、第２ターミナル液として、リーデ
イング液とターミナル液との間にターミナル液を
押しのけて導入し、リーデイング液と第２ターミ
ナル液との間に試料成分溶液を注入して等速電気
泳動させることを特徴とする等速電気泳動分析法
である。
この発明において、第２ターミナル液は、移動
度がリーデイングイオンとターミナルイオンの間
で、しかも試料成分よりも低いイオンを含む電解
液であり、具体例としては移動度が第１表に示す
ターミナル液中のターミナルイオンより大きく、
試料成分イオンよりも小さいイオンを含む電解液
である。そしてこの第２ターミナル液はリーデイ
ング液とターミナル液との間にターミナル液をタ
ーミナル電極槽側へ押しのけて導入される。導入
される第２ターミナル液の量はもとのターミナル
液が細い泳動管内とは比較にならない程大容量の
ターミナル電極槽へ押しやられ泳動管に残らない
程度が好ましい（例えば50μ、なお、ターミナ
ル液全量変更するには20〜50ml必要）。
このように第２ターミナル液の導入によつて、
もとのターミナル液（第１ターミナル液）には不
純物が含まれていて通常ターミナル液としては使
用しにくいものも使用できるということになる。
一般には移動度の小さいターミナル液が第１ター
ミナル液として好適である。等速電気泳動法によ
れば、各成分イオンが移動度の大きさの順に並ん
で泳動管を泳動する。
一方、小さい移動度のターミナル液は大きい移
動度のものに比して電気抵抗が大きい。
従つて移動度の（最も）小さいターミナルイオ
ンは、泳動管に泳動されないことになるので、泳
動管内の電気抵抗の上昇が少なく、泳動管内に高
電圧が印加された場合、通電性が良好で、温度上
昇が少ない。その結果、気泡の発生やその発熱と
気泡発生にともなう検出器における分極が防止で
き、最適電解液条件での分析が可能になる。
(ホ) 実施例
(i) まずこの発明に係る等速電気泳動分析法を実
施するための装置例を第１図に基づいて説明す
る。
等速電気泳動分析装置１は、リーデイング電
極槽２と、ターミナル電極槽３と、両電極槽を
結び、試料注入口４、検出器５及び第２ターミ
ナル液導入口６を具備した泳動細管７と、両電
極槽の泳動電源８とから主として構成されてい
る。
(ii) ギ酸、酢酸、プロピオン酸、酪酸、吉草酸、
カプロン酸の６種の脂肪酸を分析するために
は、リーデイング液として10ｍＭ塩酸＋Ｌヒス
チジン（PH6.2）・0.1％トリトンＸ−100〔登録
商標、和光純薬工業(株)製ポリオキシエチレン〕
と、ターミナル液として10ｍＭ−MES＋トリ
ス（ヒドロキシメチル）アミノメタン（PH7.0）
とが最適条件であり、第２ターミナル液の導入
は不要である（第２図参照）。
(iii) 次にマレイン酸、酒石酸、クエン酸、リンゴ
酸、乳酸、コハク酸、酢酸の７種の有機酸を分
析する場合に、第２ターミナル液を導入すれ
ば、(ii)のターミナル液を変更することなくその
まま残して最適条件で分析できる。
すなわち、上記有機酸の最適分析条件は、リ
ーデイング液として５ｍＭ塩酸＋β−アラニン
（PH3.0）・0.1％トリトンＸ−100と、第２ター
ミナル液として10ｍＭプロピオン酸ナトリウム
である。そこでリーデイング液を(ii)のものから
上記５ｍＭ塩酸＋β−アラニン（PH3.0）・0.1
％トリトンＸ−100に変更し、次いで第２ター
ミナル液導入口６から、その変更後のリーデイ
ング液と元の(ii)のターミナル液〔10ｍＭ−
MES＋トリス（ヒドロキシメチル）アミノメ
タン（PH7.0）〕との間に、ターミナル液を押し
のけて上記10ｍＭプロピオン酸ナトリウムを第
２ターミナル液として導入する。その後リーデ
イング液と第２ターミナル液との間に試料成分
（上記有機酸）を注入して泳動を行い、プロピ
オン酸イオンが検出されたところで分析を終了
する。かくして有機酸が最適電解液条件で分析
できる。第２ターミナル液の導入は、ターミナ
ル液との交換に比較して操作が簡単であり、更
に交換後の洗浄においては洗浄個所の大半は不
純物についてきびしい条件が付かないターミナ
ル液のためのターミナル電極槽なので、ほとん
ど残留イオンを無視できるという効果がある。
(iv) このような(iii)の電解液条件で、分析後、(ii)
の
電解液条件に変更する場合、プロピオン酸ナト
リウムが残存すると低級脂肪酸の分析上支障
が、生じるが第２ターミナル液としてそのプロ
ピオン酸ナトリウムを少量使用したのみである
ので、簡単な洗浄で除去できる。なお、リーデ
イング液は変更してもリーデイングイオンの塩
素は変らないので、ターミナル液の交換ほど注
意はいらない。
(v) リーデイングの電位勾配（PGL）に対する試
料のそれ（PGS）の比（RE値）を予め求めて
おけば、そのRE値でリーデイングイオンの移
動度を割ると試料の移動度が求められる。
RE値＝PGS／PGL （第４図参照）
例えばリーデイングイオンとして塩素を用い
た場合、塩素の移動度は76.35×10-5cm2
V-1sec-1（25℃）である。リーデイング液のPH
が3.0の場合は、MESのRE値は51.09であるか
ら、
76.35/51.09＝1.49（×10-5cm2V-1sec-1）
更に、リーデイング液のPHが6.2の場合は、
MESのRE値が3.80であるから、移動度は
76.35/3.80＝20.09（×10-5cm2V-1sec-1）
更にリーデイング液のPHが3.0の場合はプロ
ピオン酸のRE値は11.19であるからこのときの
移動度は、
76.35/11.19＝6.82（×10-5cm2V-1sec-1）
ここでRE値が13以上では通電性が悪く分析
上不適当であるとされている。
(ヘ) 効果
この発明は、もとのターミナル液をそのままに
して、別のターミナル液をリーデイング液ともと
のターミナル液との間に導入することによつて、
操作性よく最適電解液条件で分析が可能になる。[Table] However, such changes in analysis conditions are complicated in terms of analysis operations. (c) Purpose This invention was made in view of these circumstances, and one of its main purposes is to improve the leading fluid.
The objective is to provide an easy-to-operate method that allows analysis under optimal conditions for both liquids without changing the original terminal liquid even if the pH or composition changes. (D) Structure This invention uses an electrolytic solution containing ions whose mobility is between the leading ion and the terminal ion and whose mobility is lower than that of the sample components as a second terminal solution in isotachophoresis analysis. An isokinetic electrophoresis analysis method characterized by displacing and introducing a terminal liquid between a leading liquid and a terminal liquid, and injecting a sample component solution between a leading liquid and a second terminal liquid to perform isokinetic electrophoresis. It is. In this invention, the second terminal liquid is an electrolytic solution containing ions whose mobility is between the leading ion and the terminal ion and lower than that of the sample components. larger than the terminal ion inside,
This is an electrolytic solution containing ions smaller than the sample component ions. This second terminal liquid is introduced between the leading liquid and the terminal liquid, displacing the terminal liquid toward the terminal electrode tank. The amount of the second terminal liquid to be introduced is preferably such that the original terminal liquid is pushed into the terminal electrode tank, which has a much larger capacity than the thin electrophoresis tube, and does not remain in the electrophoresis tube (for example, 50μ, but the terminal liquid 20-50ml is required to change the total amount). In this way, by introducing the second terminal liquid,
This means that the original terminal liquid (first terminal liquid) contains impurities and is normally difficult to use as a terminal liquid, but can also be used.
Generally, a terminal liquid with low mobility is suitable as the first terminal liquid. According to isotachophoresis, each component ion migrates through an electrophoresis tube in order of its mobility. On the other hand, a terminal liquid with low mobility has a higher electrical resistance than one with high mobility. Therefore, terminal ions with (the lowest) mobility are not migrated into the migration tube, so there is little increase in electrical resistance within the migration tube, and when a high voltage is applied to the migration tube, good conductivity is achieved. There is little temperature rise. As a result, it is possible to prevent the generation of bubbles, their heat generation, and polarization in the detector due to the generation of bubbles, making it possible to perform analysis under optimal electrolyte conditions. (E) Example (i) First, an example of an apparatus for carrying out the isotachophoresis analysis method according to the present invention will be explained based on FIG. The isotachophoresis analyzer 1 includes a leading electrode tank 2, a terminal electrode tank 3, and an electrophoresis tube 7 that connects both electrode tanks and is equipped with a sample injection port 4, a detector 5, and a second terminal liquid introduction port 6. , and a migration power source 8 for both electrode tanks. (ii) formic acid, acetic acid, propionic acid, butyric acid, valeric acid,
To analyze the six types of fatty acids in caproic acid, use 10mM hydrochloric acid + L histidine (PH6.2) and 0.1% Triton X-100 as a reading solution [registered trademark, polyoxyethylene manufactured by Wako Pure Chemical Industries, Ltd.]
and 10mM-MES + tris(hydroxymethyl)aminomethane (PH7.0) as the terminal solution.
is the optimum condition, and there is no need to introduce the second terminal liquid (see Figure 2). (iii) Next, when analyzing seven types of organic acids: maleic acid, tartaric acid, citric acid, malic acid, lactic acid, succinic acid, and acetic acid, by introducing the second terminal liquid, the terminal liquid in (ii) can be analyzed. It can be left unchanged and analyzed under optimal conditions. That is, the optimum analysis conditions for the organic acid are 5mM hydrochloric acid + β-alanine (PH3.0)/0.1% Triton X-100 as the leading liquid and 10mM sodium propionate as the second terminal liquid. Therefore, I changed the reading solution from (ii) to the above 5mM hydrochloric acid + β-alanine (PH3.0), 0.1
% Triton
MES + tris(hydroxymethyl)aminomethane (PH7.0)], the terminal liquid was pushed away and the above 10 mM sodium propionate was introduced as a second terminal liquid. Thereafter, a sample component (the above-mentioned organic acid) is injected between the leading liquid and the second terminal liquid, electrophoresis is performed, and the analysis is terminated when propionate ions are detected. In this way, organic acids can be analyzed under optimal electrolyte conditions. Introducing the second terminal liquid is easier to operate than replacing it with the terminal liquid, and most of the cleaning areas after replacement are the terminal electrode tank for the terminal liquid, which is not subject to strict conditions regarding impurities. Therefore, the effect is that residual ions can be almost ignored. (iv) After analysis under these electrolyte conditions of (iii), (ii)
When changing the electrolyte conditions to the above, residual sodium propionate will cause problems in the analysis of lower fatty acids, but since only a small amount of sodium propionate is used as the second terminal solution, it can be removed by simple washing. Note that even if you change the leading liquid, the chlorine in the leading ion will not change, so you do not need to be as careful as changing the terminal liquid. (v) If the ratio (RE value) of the leading potential gradient (PG L ) to that of the sample (PG S ) is determined in advance, the mobility of the sample can be determined by dividing the mobility of the leading ion by that RE value. It will be done. RE value = PG S / PG L (see Figure 4) For example, when chlorine is used as the leading ion, the mobility of chlorine is 76.35×10 -5 cm 2
V -1 sec -1 (25℃). Leading fluid PH
When is 3.0, the RE value of MES is 51.09, so 76.35/51.09=1.49 (×10 -5 cm 2 V -1 sec -1 ) Furthermore, if the pH of the leading liquid is 6.2,
Since the RE value of MES is 3.80, the mobility is 76.35/3.80 = 20.09 (×10 -5 cm 2 V -1 sec -1 ) Furthermore, if the pH of the leading liquid is 3.0, the RE value of propionic acid is 11.19. Therefore, the mobility at this time is 76.35/11.19 = 6.82 (×10 -5 cm 2 V -1 sec -1 ) Here, if the RE value is 13 or more, the conductivity is poor and it is considered inappropriate for analysis. . (F) Effect This invention allows the original terminal liquid to remain as it is and introduces another terminal liquid between the leading liquid and the original terminal liquid.
It is easy to operate and enables analysis under optimal electrolyte conditions.
第１図は、この発明に係る等速電気泳動分析法
を実施するための装置例の説明図、第２図は脂肪
酸の分析例を示すイソタコフエログラム、第３図
は有機酸の分析例を示すイソタコフエログラム
（第２ターミナル液導入）、第４図はRE値の定義
を説明するためのイソタコフエログラムである。
FIG. 1 is an explanatory diagram of an example of an apparatus for carrying out the isotachophoresis analysis method according to the present invention, FIG. 2 is an isotacopherogram showing an example of analysis of fatty acids, and FIG. 3 is an example of analysis of organic acids. Figure 4 is an isotacopherogram showing the definition of the RE value (second terminal liquid introduced).
Claims (1)
デイングイオンとターミナルイオンの間で、しか
も試料成分よりも低い移動度のイオンを含む電解
液を、第２ターミナル液として、リーデイング液
とターミナル液との間にターミナル液を押しのけ
て導入し、リーデイング液と第２ターミナル液と
の間に試料成分溶液を注入して等速電気泳動させ
ることを特徴とする等速電気泳動分析法。1 In isotachophoresis analysis, an electrolytic solution containing ions whose mobility is between the leading ion and the terminal ion and lower than that of the sample components is used as the second terminal solution, and the electrolytic solution is used as the second terminal solution. An isokinetic electrophoresis analysis method characterized by displacing and introducing a terminal liquid between the leading liquid and the second terminal liquid, and injecting a sample component solution between the leading liquid and the second terminal liquid to perform isokinetic electrophoresis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP58250168A JPS60142243A (en) | 1983-12-28 | 1983-12-28 | Isokinetic electrophoresis analysis method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP58250168A JPS60142243A (en) | 1983-12-28 | 1983-12-28 | Isokinetic electrophoresis analysis method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS60142243A JPS60142243A (en) | 1985-07-27 |
| JPH0524455B2 true JPH0524455B2 (en) | 1993-04-07 |
Family
ID=17203826
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP58250168A Granted JPS60142243A (en) | 1983-12-28 | 1983-12-28 | Isokinetic electrophoresis analysis method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS60142243A (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61194363A (en) * | 1985-02-25 | 1986-08-28 | Hitachi Ltd | Nucleic acid fragment detection device |
| WO2008053047A2 (en) * | 2006-11-01 | 2008-05-08 | Becton, Dickinson & Company | Methods and devices for isotachophoresis applications |
| US8580097B2 (en) * | 2009-04-27 | 2013-11-12 | Wako Pure Chemical Industries, Ltd. | Isotachophoresis of blood-derived samples |
| CN114624323B (en) * | 2020-12-11 | 2026-03-10 | 中国科学院大连化学物理研究所 | Device and method for detecting acetic acid and lactic acid in Daqu and fermented grains |
-
1983
- 1983-12-28 JP JP58250168A patent/JPS60142243A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS60142243A (en) | 1985-07-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Beckers et al. | Isotachophoresis with two leading ions and migration behaviour in capillary zone electrophoresis: II. Migration behaviour in capillary zone electrophoresis | |
| Schwer et al. | Analytical and micropreparative separation of peptides by capillary zone electrophoresis using discontinuous buffer systems | |
| Tseng et al. | On-line concentration and separation of proteins by capillary electrophoresis using polymer solutions | |
| Arnett et al. | Investigation of the mechanism of pH‐mediated stacking of anions for the analysis of physiological samples by capillary electrophoresis | |
| Dolnik et al. | Capillary zone electrophoresis of dilute samples with isotachophoretic preconcentration | |
| Bobbitt et al. | Chemiluminescent detection of amines and amino acids using in situ generated Ru (bpy) 33+ following separation by capillary electrophoresis | |
| Xu et al. | Determination of purine bases by capillary zone electrophoresis with wall-jet amperometric detection | |
| Huang et al. | Determination of sugars by capillary electrophoresis with electrochemical detection using cuprous oxide modified electrodes | |
| Boss et al. | Multiple sequential fraction collection of peptides and glycopeptides by high-performance capillary electrophoresis | |
| US5614073A (en) | Method and apparatus for detection of underivatized amines and amino acids utilizing end column addition of Ru(bpy)32+ | |
| JPH0524455B2 (en) | ||
| Šutáček et al. | Simple method for generation of dymanic pH gradient in capillary zone electrophoresis | |
| Gebauer et al. | Optimization in isotachophoresis: The concept of selectivity and separation speed | |
| Lagane et al. | Capillary electrophoresis: theory, teaching approach and separation of oligosaccharides using indirect UV detection | |
| Foret et al. | Ionic boundaries in biological capillary electrophoresis | |
| Thormann et al. | Impact of electroosmosis on isotachophoresis in open‐tubular fused‐silica capillaries: Analysis of the evolution of a stationary steady‐state zone structure by computer simulation and experimental validation | |
| Nath et al. | Correlation of migration behavior in free‐flow zone electrophoresis and electrophoretic titration curve | |
| Lee et al. | Analytical and preparative capillary zone electrophoresis of opioid peptides | |
| JP3486981B2 (en) | Liquid sample concentration method and liquid sample concentration device | |
| JPH0743351B2 (en) | Electrophoresis buffer and capillary electrophoresis | |
| JPS58168957A (en) | Electrophoresis device | |
| Perrett et al. | Capillary electrophoresis for the analysis of cellular nucleotides | |
| JPH04160356A (en) | Sample injection device of capillary electrophoretic apparatus | |
| Cooper et al. | Gel protein capillary extraction apparatus. Electronic protein transfer | |
| Zhang et al. | Determination of histamine by capillary zone electrophoresis with amperometric detection |