JPH053845B2 - - Google Patents
Info
- Publication number
- JPH053845B2 JPH053845B2 JP60046255A JP4625585A JPH053845B2 JP H053845 B2 JPH053845 B2 JP H053845B2 JP 60046255 A JP60046255 A JP 60046255A JP 4625585 A JP4625585 A JP 4625585A JP H053845 B2 JPH053845 B2 JP H053845B2
- Authority
- JP
- Japan
- Prior art keywords
- present
- skin
- triterpenes
- hederagenin
- formula
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 150000003648 triterpenes Chemical class 0.000 claims description 17
- 238000002360 preparation method Methods 0.000 claims description 8
- GCGBHJLBFAPRDB-KCVAUKQGSA-N Scutellaric acid Natural products CC1(C)CC[C@@]2(CC[C@@]3(C)[C@@H]4CC[C@H]5[C@@](C)(CO)[C@H](O)CC[C@]5(C)[C@H]4CC=C3[C@@H]2C1)C(=O)O GCGBHJLBFAPRDB-KCVAUKQGSA-N 0.000 description 14
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 12
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 12
- 230000000694 effects Effects 0.000 description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 11
- 239000012071 phase Substances 0.000 description 9
- NTWLPZMPTFQYQI-UHFFFAOYSA-N (3alpha)-olean-12-ene-3,23-diol Natural products C1CC(O)C(C)(CO)C2CCC3(C)C4(C)CCC5(C)CCC(C)(C)CC5C4=CCC3C21C NTWLPZMPTFQYQI-UHFFFAOYSA-N 0.000 description 8
- GCGBHJLBFAPRDB-UHFFFAOYSA-N Hederagenin Natural products CC1(C)CCC2(CCC3(C)C4CCC5C(C)(CO)C(O)CCC5(C)C4CC=C3C2C1)C(=O)O GCGBHJLBFAPRDB-UHFFFAOYSA-N 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 8
- PGOYMURMZNDHNS-MYPRUECHSA-N hederagenin Chemical compound C1C[C@H](O)[C@@](C)(CO)[C@@H]2CC[C@@]3(C)[C@]4(C)CC[C@@]5(C(O)=O)CCC(C)(C)C[C@H]5C4=CC[C@@H]3[C@]21C PGOYMURMZNDHNS-MYPRUECHSA-N 0.000 description 8
- 206010013786 Dry skin Diseases 0.000 description 7
- 239000008213 purified water Substances 0.000 description 7
- 210000003491 skin Anatomy 0.000 description 7
- QMHCWDVPABYZMC-UHFFFAOYSA-N 3-Hydroxy-23-oxo-olean-12-en-28-saeure Natural products C1CC(O)C(C)(C=O)C2CCC3(C)C4(C)CCC5(C(O)=O)CCC(C)(C)CC5C4=CCC3C21C QMHCWDVPABYZMC-UHFFFAOYSA-N 0.000 description 6
- QMHCWDVPABYZMC-MYPRUECHSA-N 3beta-hydroxy-23-oxoolean-12-en-28-oic acid Chemical compound C1C[C@H](O)[C@@](C)(C=O)[C@@H]2CC[C@@]3(C)[C@]4(C)CC[C@@]5(C(O)=O)CCC(C)(C)C[C@H]5C4=CC[C@@H]3[C@]21C QMHCWDVPABYZMC-MYPRUECHSA-N 0.000 description 6
- PAIBKVQNJKUVCE-UHFFFAOYSA-N Gypsogeninsaeure Natural products C1CC(O)C(C)(C(O)=O)C2CCC3(C)C4(C)CCC5(C(O)=O)CCC(C)(C)CC5C4=CCC3C21C PAIBKVQNJKUVCE-UHFFFAOYSA-N 0.000 description 6
- 239000003205 fragrance Substances 0.000 description 6
- QMHCWDVPABYZMC-RFVOPWELSA-N gypsogenin Natural products CC1(C)CC[C@@]2(CC[C@]3(C)C(=CC[C@@H]4[C@@]5(C)CC[C@H](O)[C@](C)(C=O)[C@H]5CC[C@@]34C)[C@H]2C1)C(=O)O QMHCWDVPABYZMC-RFVOPWELSA-N 0.000 description 6
- 238000003756 stirring Methods 0.000 description 6
- 239000002537 cosmetic Substances 0.000 description 5
- 239000003921 oil Substances 0.000 description 5
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 4
- 239000000284 extract Substances 0.000 description 4
- 239000004615 ingredient Substances 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- LXCFILQKKLGQFO-UHFFFAOYSA-N methylparaben Chemical compound COC(=O)C1=CC=C(O)C=C1 LXCFILQKKLGQFO-UHFFFAOYSA-N 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 230000002265 prevention Effects 0.000 description 4
- 230000029663 wound healing Effects 0.000 description 4
- VBICKXHEKHSIBG-UHFFFAOYSA-N beta-monoglyceryl stearate Natural products CCCCCCCCCCCCCCCCCC(=O)OCC(O)CO VBICKXHEKHSIBG-UHFFFAOYSA-N 0.000 description 3
- 230000020411 cell activation Effects 0.000 description 3
- 239000006071 cream Substances 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 239000006210 lotion Substances 0.000 description 3
- GLDOVTGHNKAZLK-UHFFFAOYSA-N octadecan-1-ol Chemical compound CCCCCCCCCCCCCCCCCCO GLDOVTGHNKAZLK-UHFFFAOYSA-N 0.000 description 3
- 239000002674 ointment Substances 0.000 description 3
- 239000001651 pyrus cydonia seed extract Substances 0.000 description 3
- 210000004927 skin cell Anatomy 0.000 description 3
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 244000163122 Curcuma domestica Species 0.000 description 2
- 235000003392 Curcuma domestica Nutrition 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 239000002202 Polyethylene glycol Substances 0.000 description 2
- 239000004372 Polyvinyl alcohol Substances 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 235000013871 bee wax Nutrition 0.000 description 2
- 239000012166 beeswax Substances 0.000 description 2
- 230000004663 cell proliferation Effects 0.000 description 2
- 230000005757 colony formation Effects 0.000 description 2
- 235000003373 curcuma longa Nutrition 0.000 description 2
- DTPCFIHYWYONMD-UHFFFAOYSA-N decaethylene glycol Polymers OCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO DTPCFIHYWYONMD-UHFFFAOYSA-N 0.000 description 2
- 239000002552 dosage form Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 150000002148 esters Chemical class 0.000 description 2
- 229960001617 ethyl hydroxybenzoate Drugs 0.000 description 2
- 235000010228 ethyl p-hydroxybenzoate Nutrition 0.000 description 2
- 239000004403 ethyl p-hydroxybenzoate Substances 0.000 description 2
- NUVBSKCKDOMJSU-UHFFFAOYSA-N ethylparaben Chemical compound CCOC(=O)C1=CC=C(O)C=C1 NUVBSKCKDOMJSU-UHFFFAOYSA-N 0.000 description 2
- 210000002950 fibroblast Anatomy 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- BXWNKGSJHAJOGX-UHFFFAOYSA-N hexadecan-1-ol Chemical compound CCCCCCCCCCCCCCCCO BXWNKGSJHAJOGX-UHFFFAOYSA-N 0.000 description 2
- 235000010270 methyl p-hydroxybenzoate Nutrition 0.000 description 2
- 239000004292 methyl p-hydroxybenzoate Substances 0.000 description 2
- 229960002216 methylparaben Drugs 0.000 description 2
- 229930014626 natural product Natural products 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 229920002451 polyvinyl alcohol Polymers 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- PRAKJMSDJKAYCZ-UHFFFAOYSA-N squalane Chemical compound CC(C)CCCC(C)CCCC(C)CCCCC(C)CCCC(C)CCCC(C)C PRAKJMSDJKAYCZ-UHFFFAOYSA-N 0.000 description 2
- 235000013976 turmeric Nutrition 0.000 description 2
- IDQVFXZQPGAVAM-UHFFFAOYSA-N (2alpha,3beta,6beta)-2,3,6,23-Tetrahydroxy-12-oleanen-28-oic acid Natural products C1C(O)C(O)C(C)(CO)C2C(O)CC3(C)C4(C)CCC5(C(O)=O)CCC(C)(C)CC5C4=CCC3C21C IDQVFXZQPGAVAM-UHFFFAOYSA-N 0.000 description 1
- 229940058015 1,3-butylene glycol Drugs 0.000 description 1
- 241001116389 Aloe Species 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 239000002211 L-ascorbic acid Substances 0.000 description 1
- 235000000069 L-ascorbic acid Nutrition 0.000 description 1
- 239000004166 Lanolin Substances 0.000 description 1
- 240000007817 Olea europaea Species 0.000 description 1
- 235000002725 Olea europaea Nutrition 0.000 description 1
- APTNOIWSCDBIAS-UHFFFAOYSA-N Platycodigenin Natural products C1C(O)C(O)C(CO)(CO)C2CCC3(C)C4(C)CC(O)C5(C(O)=O)CCC(C)(C)CC5C4=CCC3C21C APTNOIWSCDBIAS-UHFFFAOYSA-N 0.000 description 1
- 229920001219 Polysorbate 40 Polymers 0.000 description 1
- 206010072170 Skin wound Diseases 0.000 description 1
- 235000021355 Stearic acid Nutrition 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000005377 adsorption chromatography Methods 0.000 description 1
- 235000011399 aloe vera Nutrition 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 235000019437 butane-1,3-diol Nutrition 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 229940082500 cetostearyl alcohol Drugs 0.000 description 1
- 229960000541 cetyl alcohol Drugs 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 239000012259 ether extract Substances 0.000 description 1
- 239000012091 fetal bovine serum Substances 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 239000003906 humectant Substances 0.000 description 1
- 235000019388 lanolin Nutrition 0.000 description 1
- 229940039717 lanolin Drugs 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- JXTPJDDICSTXJX-UHFFFAOYSA-N n-Triacontane Natural products CCCCCCCCCCCCCCCCCCCCCCCCCCCCCC JXTPJDDICSTXJX-UHFFFAOYSA-N 0.000 description 1
- GOQYKNQRPGWPLP-UHFFFAOYSA-N n-heptadecyl alcohol Natural products CCCCCCCCCCCCCCCCCO GOQYKNQRPGWPLP-UHFFFAOYSA-N 0.000 description 1
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- IDQVFXZQPGAVAM-YPRSBIJBSA-N protobassic acid Chemical compound C1[C@H](O)[C@H](O)[C@@](C)(CO)[C@@H]2[C@H](O)C[C@@]3(C)[C@]4(C)CC[C@@]5(C(O)=O)CCC(C)(C)C[C@H]5C4=CC[C@@H]3[C@]21C IDQVFXZQPGAVAM-YPRSBIJBSA-N 0.000 description 1
- 238000010898 silica gel chromatography Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 229940032094 squalane Drugs 0.000 description 1
- 239000008117 stearic acid Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- OULAJFUGPPVRBK-UHFFFAOYSA-N tetratriacontyl alcohol Natural products CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCO OULAJFUGPPVRBK-UHFFFAOYSA-N 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 229940099259 vaseline Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/63—Steroids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Dermatology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Cosmetics (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Description
【発明の詳細な説明】
［産業上の利用分野］
本発明はトリテルペンの１種又は２種を配合す
ることにより、創傷治癒、肌荒れ防止、肌荒れ改
善および、細胞賦活効果に優れた皮膚外用剤に関
する。Detailed Description of the Invention [Industrial Field of Application] The present invention relates to an external skin preparation that is excellent in wound healing, prevention of skin roughness, improvement of skin roughness, and cell activation effects by incorporating one or two types of triterpenes. .
［従来の技術］
従来、アロエ、ヒレハリ草、シコン等あるいは
それらの抽出物を配合することにより創傷治癒や
肌荒れ防止の効果を目的とする外用剤等があるが
いまだ満足できるものがなかつた。[Prior Art] Conventionally, there have been external preparations that contain aloe, turmeric, turmeric, etc., or their extracts, for the purpose of wound healing and prevention of skin roughness, but none have yet been satisfactory.
［発明が解決しようとする問題点］
本発明者らは、こうした事情にかんがみ、創傷
治癒、肌荒れ防止、肌荒れ改善、細胞賦活の効果
に優れた皮膚外用剤を得るべく鋭意研究を重ねた
結果、特定のトリテルペン類を配合した皮膚外用
剤が、上記目的を達成することを見いだし、この
知見にもとづいて本発明を完成するに至つた。[Problems to be Solved by the Invention] In view of these circumstances, the present inventors have conducted intensive research to obtain a skin external preparation that is excellent in wound healing, prevention of skin roughness, improvement of skin roughness, and cell activation. It has been discovered that an external preparation for skin containing specific triterpenes can achieve the above object, and based on this knowledge, the present invention has been completed.
［問題点を解決するための手段］
すなわち、本発明は下記一般式で表わされるト
リテルペン類の１種又は２種を配合することを特
徴とする皮膚外用剤である。[Means for Solving the Problems] That is, the present invention is an external skin preparation characterized by containing one or two triterpenes represented by the following general formula.
（式中 R1＝CH2OHまたはCHO， R2＝CH3又は、CH2OH， R3＝Ｈ又はOH，R4＝Ｈ又は、OH R5＝Ｈ又はOH， R6＝CH3を表す。） 以下本発明の構成について詳述する。 (In the formula, R 1 = CH 2 OH or CHO, R 2 = CH 3 or CH 2 OH, R 3 = H or OH, R 4 = H or OH R 5 = H or OH, R 6 = CH 3 ) The configuration of the present invention will be described in detail below.
本発明に用いるトリテルペン類は、上記一般式
で表わされるものであり、上記トリテルペン類と
しては、例えば、ヘデラゲニン｛Hederagenin
（式中R1＝CH2OH，R2＝CH3，R3＝R4＝R5＝
Ｈ）｝，ギプソゲニン｛Gypsogenin（式中R3＝R4
＝R5＝Ｈ，R1＝CHO，R2＝CH3）｝プラチコゲ
ニン｛Platycodigenin（式中R1＝R2＝CH2OH，
R3＝R5＝OH，R4＝Ｈ）｝，プロトバシツクアシ
ツド｛Protobassic acid（式中R1＝CH2OH，R3
＝OH，R4＝OH，R5＝Ｈ，R2＝R6＝CH3），等
があげられるがこれらの中でヘデラゲニン、ある
いはギプソゲニンが最も好ましい。 The triterpenes used in the present invention are represented by the above general formula, and examples of the triterpenes include hederagenin.
(In the formula, R 1 = CH 2 OH, R 2 = CH 3 , R 3 = R 4 = R 5 =
H)}, Gypsogenin (in the formula R 3 = R 4
= R 5 = H, R 1 = CHO, R 2 = CH 3 )} Platycodigenin (in the formula R 1 = R 2 = CH 2 OH,
R 3 = R 5 = OH, R 4 = H)}, Protobassic acid (in the formula R 1 = CH 2 OH, R 3
=OH, R4 =OH, R5 =H, R2 = R6 = CH3 ), among which hederagenin or gypsogenin is most preferred.
本発明に用いられる上記トリテルペン類は合成
品でも天然物の抽出物でも良い。天然物の抽出物
の場合は例えば以下のような方法で得られる。 The triterpenes used in the present invention may be synthetic products or extracts of natural products. Extracts of natural products can be obtained, for example, by the following method.
延命皮、キキヨウ、オリーブ葉、チヨウジの芽
等の植物を溶媒、例えば熱水、水ー低級アルコー
ル、低級アルコール、エテールなどで抽出して得
られる抽出液を濃縮した後、硫酸等の酸で加水分
解したのちエーテル抽出、濃縮して粗トリテルペ
ン類を得る。トリテルペン類を単離するにはシリ
カゲルクロマトグラフイーなどの吸着系クロマト
グラフイーを用いて分画すればよいが、本発明の
実施にあたつては、上記エーテル抽出物の粗トリ
テルペンとして配合しても良いし単離精製したト
リテルペンとして配合しても良い。 The extract obtained by extracting plants such as Enmeihiki, Kikiyo, olive leaf, and Chiyoji buds with a solvent such as hot water, water-lower alcohol, lower alcohol, ether, etc. is concentrated, and then hydrated with an acid such as sulfuric acid. After decomposition, it is extracted with ether and concentrated to obtain crude triterpenes. Triterpenes can be isolated by fractionation using adsorption chromatography such as silica gel chromatography, but in carrying out the present invention, triterpenes are blended as crude triterpenes in the above ether extract. It may also be blended as an isolated and purified triterpene.
本発明の実施にあたつては、これらのトリテル
ペンの内、１種又は２種以上が適宜選択され配合
される。配合量は化粧料全量中固形分として、
0.001〜５重量％である。0.001重量％以下である
と、本発明でいう効果が十分に発揮されず、好ま
しくない。 In carrying out the present invention, one or more of these triterpenes are appropriately selected and blended. The amount is based on the solid content of the total amount of cosmetics.
It is 0.001 to 5% by weight. If it is less than 0.001% by weight, the effects of the present invention will not be sufficiently exhibited, which is not preferable.
本発明の化粧料は前記の必須成分に加えて必要
に応じて、本発明の効果を損なわない範囲内で、
化粧品、医薬品等の皮膚外用剤に一般に用いられ
る各種成分、すなわち、粉末、油分、界面活性
剤、保湿剤、増粘剤、防腐剤、酸化防止剤、香
料、色剤、薬剤等を配合することができる。薬剤
の中でも特にＬ−アスコルビン酸又はそのエステ
ルを配合した時、顕著な効果が発揮されるので好
ましい。また本発明の化粧料の剤型は任意であ
り、例えば化粧水等の可溶化系、乳液、クリーム
等の乳化系あるいは軟膏、分散液、などの剤型を
とることができる。 In addition to the above-mentioned essential ingredients, the cosmetic of the present invention may contain, if necessary, within a range that does not impair the effects of the present invention.
Incorporating various ingredients commonly used in external skin preparations such as cosmetics and pharmaceuticals, such as powders, oils, surfactants, humectants, thickeners, preservatives, antioxidants, fragrances, colorants, and drugs. I can do it. Among the drugs, L-ascorbic acid or its ester is particularly preferred since it exhibits remarkable effects. Further, the dosage form of the cosmetic of the present invention is arbitrary, and can be, for example, a solubilized system such as a lotion, an emulsified system such as a milky lotion or a cream, or a dosage form such as an ointment or a dispersion.
［発明の効果］
本発明の皮膚外用剤は皮膚の創傷治癒、肌荒れ
防止、肌荒れ改善効果及び細胞賦活効果に優れた
ものである。次ぎにこれらの効果を総括的に表わ
す皮膚細胞増殖促進作用について実施例をあげて
説明する。[Effects of the Invention] The skin external preparation of the present invention is excellent in skin wound healing, skin roughness prevention, skin roughness improvement effects, and cell activation effects. Next, the effect of promoting skin cell proliferation, which comprehensively represents these effects, will be explained with reference to Examples.
（皮膚細胞増殖促進作用）
ヒト皮膚組織を細片し、細胞培養用シヤーレの
底面に附着させてEagle′s MEM培養液（10％牛
胎児血清含有）中で１週間培養するとシヤーレの
底面がほぼ全面に線維芽細胞で満たされる。この
線維芽細胞を0.25％トリプシン溶液で処理するこ
とによつて単一細胞とし、次に10000コ細胞／ml
の細胞浮遊液をつくり、この溶液をシヤーレ当た
り0.1ml加え、Eagle′s MEM培養液及びトリテル
ペン類（最終濃度5μg／ml）を更に加えてCO2−
インキユベター中で２週間培養し、その後細胞固
定して染色した後、細胞のコロニーを計測した。
なおトリテルペン類を添加しない場合をコントロ
ールとした。結果を第１図に示す。コロニー形成
率は次式によつて算出した。(Skin cell growth promotion effect) When human skin tissue was cut into small pieces, attached to the bottom of a cell culture shear dish, and cultured for one week in Eagle's MEM culture solution (containing 10% fetal bovine serum), the bottom of the shear dish almost disappeared. The entire surface is filled with fibroblasts. The fibroblasts were made into single cells by treatment with 0.25% trypsin solution and then reduced to 10000 cells/ml.
Prepare a cell suspension of
After culturing in an incubator for two weeks, the cells were fixed and stained, and then cell colonies were counted.
Note that the case in which no triterpenes were added was used as a control. The results are shown in Figure 1. Colony formation rate was calculated using the following formula.
コロニー形成率＝Ｔ／Ｃ×100（％）
Ｔ＝トリテルペンを処理した細胞のコロニー数／シヤー
レ当たりの植え込み細胞数
Ｃ＝
トリテルペンを処理しない細胞のコロニー数／シヤーレ
当たりの植え込み細胞数
第１図に示す如く、トリテルペンであるヘデラ
ゲニンあるいはギプソゲニンを添加したものはコ
ントロールに比べていずれも著名な効果を示し
た。 Colony formation rate = T/C x 100 (%) T = number of colonies of cells treated with triterpene/number of implanted cells per shear C =
Number of colonies of cells not treated with triterpene/Number of implanted cells per shear plate As shown in Figure 1, the addition of triterpene hederagenin or gypsogenin all showed remarkable effects compared to the control.
［実施例］
次に実施例によつて本発明をさらに詳細に説明
する。尚、本発明はこれにより限定されるもので
はない。配合量は重量％である。[Example] Next, the present invention will be explained in more detail with reference to Examples. Note that the present invention is not limited to this. The blending amount is in weight%.
実施例１クリーム
(1) セトステアリルアルコール 3.5
(2) スクワラン 40.0
(3) ミツロウ 3.0
(4) 還元ラノリン 5.0
(5) エチルパラベン 0.3
(6) ポリオキシエチレン（20）ソルビタンモノパ
ルミチン酸エステル 2.0
(7) ステアリン酸モノグリセリド 2.0
(8) ヘデラゲニン 1.0
(9) 香料 0.03
(10) １，３−ブチレングリコール 5.0
(11) グリセリン 5.0
(12) 精製水 33.17
（製法）
(1)(2)(3)(4)(5)(6)(7)(8)と(9)を加熱溶解し75℃に
保つ
たものを、75℃に加温した(10)とに攪拌しなが
ら加える。ホモミキサー処理し乳化粒子を細かく
した後、攪拌しながら急冷し、クリームを得た。Example 1 Cream (1) Cetostearyl alcohol 3.5 (2) Squalane 40.0 (3) Beeswax 3.0 (4) Reduced lanolin 5.0 (5) Ethylparaben 0.3 (6) Polyoxyethylene (20) Sorbitan monopalmitate ester 2.0 (7) ) Stearic acid monoglyceride 2.0 (8) Hederagenin 1.0 (9) Fragrance 0.03 (10) 1,3-butylene glycol 5.0 (11) Glycerin 5.0 (12) Purified water 33.17 (Production method) (1)(2)(3)(4 )(5)(6)(7)(8) and (9) were dissolved by heating and kept at 75°C, and then added to (10) heated to 75°C with stirring. After processing with a homomixer to make emulsified particles fine, the mixture was rapidly cooled while stirring to obtain cream.
実施例２乳液
(1) ギプソゲニン 0.001
(2) ステアリン酸化 1.5
(3) セチルアルコール 0.5
(4) ミツロウ 2.0
(5) ポリオキシエチレン（10）モノオレイン酸エ
ステル 1.0
(6) グリセリンモノステアリン酸エステル 1.0
(7) クインスシード抽出物（５％水溶液） 20.0
(8) プロピレングリコール 5.0
(9) エタノール 3.0
(10) エチルパラベン 0.3
(11) 香料 0.03
(12) 精製水 残余
（製法）
エタノールにギプソゲニン、香料を加えて溶解
する（アルコール相）。Example 2 Emulsion (1) Gypsogenin 0.001 (2) Stearic acid 1.5 (3) Cetyl alcohol 0.5 (4) Beeswax 2.0 (5) Polyoxyethylene (10) Monooleate 1.0 (6) Glycerin monostearate 1.0 ( 7) Quince seed extract (5% aqueous solution) 20.0 (8) Propylene glycol 5.0 (9) Ethanol 3.0 (10) Ethylparaben 0.3 (11) Fragrance 0.03 (12) Purified water Residual (manufacturing method) Gypsogenin and fragrance added to ethanol (alcohol phase).
精製水にプロピレングリコールを加え加熱溶解
して70℃に保つ（水相）。クインスシード抽出物
を除く他の成分を混合し、加熱溶解して70℃に保
つ（油相）。水相に油相を加え予備乳化を行い、
ホモミキサーで均一に乳化する。これを攪拌しな
がらアルコール相とクインスシード抽出物を加え
る。その後攪拌しながら30℃に冷却して乳液を得
た。 Add propylene glycol to purified water, heat to dissolve, and keep at 70℃ (water phase). Mix other ingredients except quince seed extract, heat and dissolve and keep at 70℃ (oil phase). Pre-emulsification is performed by adding the oil phase to the water phase.
Uniformly emulsify with a homomixer. Add the alcohol phase and quince seed extract while stirring. Thereafter, the mixture was cooled to 30°C while stirring to obtain a milky lotion.
実施例３パツク
(1) ヘデラゲニン 0.1
(2) ポリビニルアルコール 15.0
(3) ポリエチレングリコール 3.0
(4) プロピレングリコール 7.0
(5) エタノール 10.0
(6) メチルパラベン 0.05
(7) 香料 0.05
(8) 精製水 64.80
（製法）
精製水にポリエチレングリコール、プロピレン
グリコール、メチルパラベンを加え攪拌溶解す
る。つぎにポリビニルアルコールを加え加熱攪拌
し、ヘデラゲニン、香料を溶解したエタノールを
加え攪拌溶解してパツクを得た。Example 3 Pack (1) Hederagenin 0.1 (2) Polyvinyl alcohol 15.0 (3) Polyethylene glycol 3.0 (4) Propylene glycol 7.0 (5) Ethanol 10.0 (6) Methylparaben 0.05 (7) Fragrance 0.05 (8) Purified water 64.80 (Production method ) Add polyethylene glycol, propylene glycol, and methylparaben to purified water and stir to dissolve. Next, polyvinyl alcohol was added and stirred while heating, and ethanol in which hederagenin and fragrance were dissolved was added and dissolved with stirring to obtain a pack.
実施例４軟膏
(1) ヘデラゲニン 5.0
(2) ステアリルアルコール 18.0
(3) モクロウ 20.0
(4) ポリオキシエチレン（10）モノオレイン酸エ
ステル 0.25
(5) グリセリンモノステアリン酸エステル 0.25
(6) ワセリン 40.0
(7) 精製水 16.5
（製法）
精製水を70℃に保ち（水相）。他の成分を70℃
にて混合溶解する（油相）。水相に油相を加え、
ホモミキサーで均一に乳化後冷却して軟膏を得
た。Example 4 Ointment (1) Hederagenin 5.0 (2) Stearyl alcohol 18.0 (3) Mokuro 20.0 (4) Polyoxyethylene (10) Monooleate 0.25 (5) Glycerin monostearate 0.25 (6) Vaseline 40.0 (7) ) Purified water 16.5 (Production method) Keep purified water at 70℃ (aqueous phase). Other ingredients at 70℃
Mix and dissolve (oil phase). Add the oil phase to the water phase,
The mixture was uniformly emulsified using a homomixer and cooled to obtain an ointment.
第１図は本発明に係る化粧料の皮膚細胞増殖効
果を示すグラフである。
試料１：コントロール
試料２：ヘデラゲニン
試料３：ギプソゲニン
FIG. 1 is a graph showing the skin cell proliferation effect of the cosmetic according to the present invention. Sample 1: Control Sample 2: Hederagenin Sample 3: Gypsogenin
Claims (1)
又は２種を0.001〜５重量％配合すること特徴と
する皮膚外用剤。 （式中、R1＝CH2OHまたはCHO R2＝CH3 R3＝R4＝R5＝Ｈ R6＝CH3 を表す。[Scope of Claims] 1. An external skin preparation characterized by containing 0.001 to 5% by weight of one or two triterpenes represented by the following general formula. (In the formula, R 1 = CH 2 OH or CH R 2 = CH 3 R 3 = R 4 = R 5 = H R 6 = CH 3 .
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4625585A JPS61205204A (en) | 1985-03-08 | 1985-03-08 | External preparation for skin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4625585A JPS61205204A (en) | 1985-03-08 | 1985-03-08 | External preparation for skin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS61205204A JPS61205204A (en) | 1986-09-11 |
| JPH053845B2 true JPH053845B2 (en) | 1993-01-18 |
Family
ID=12742071
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP4625585A Granted JPS61205204A (en) | 1985-03-08 | 1985-03-08 | External preparation for skin |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS61205204A (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6442411A (en) * | 1987-08-11 | 1989-02-14 | Inabata Koryo Co Ltd | Cosmetic composition |
| FR2756178B1 (en) * | 1996-11-27 | 1999-05-07 | Cs | MULTIPLE L / H / L EMULSION, PREPARATION METHOD AND TOPICAL USE |
| FR2802096A1 (en) * | 1999-12-10 | 2001-06-15 | Oreal | COMPOSITION, ESPECIALLY COSMETIC, COMPRISING TRITERPENE PENTACYCLIC ACID |
| JP2001213778A (en) * | 2000-02-03 | 2001-08-07 | Pola Chem Ind Inc | Load stress mitigating preparation and skin care preparation including it |
| JP4334956B2 (en) * | 2003-09-18 | 2009-09-30 | 株式会社ノエビア | Cell activators, whitening agents, and antioxidants |
| JP2006176425A (en) * | 2004-12-21 | 2006-07-06 | Maruzen Pharmaceut Co Ltd | Collagen production promoter and skin care preparation |
| DE102009047092A1 (en) | 2009-11-24 | 2011-05-26 | Birken Gmbh | Use of a triterpenhaltigen oleogel for wound healing |
| CN111356479A (en) | 2018-01-04 | 2020-06-30 | 安瑞特研究有限公司 | Birch bark extract containing betulin and preparations thereof |
-
1985
- 1985-03-08 JP JP4625585A patent/JPS61205204A/en active Granted
Non-Patent Citations (1)
| Title |
|---|
| RIV ITAL ESSENZE.PROFUMI.PIANTE OFF=1975 * |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS61205204A (en) | 1986-09-11 |
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