JPH0543356B2 - - Google Patents
Info
- Publication number
- JPH0543356B2 JPH0543356B2 JP10173184A JP10173184A JPH0543356B2 JP H0543356 B2 JPH0543356 B2 JP H0543356B2 JP 10173184 A JP10173184 A JP 10173184A JP 10173184 A JP10173184 A JP 10173184A JP H0543356 B2 JPH0543356 B2 JP H0543356B2
- Authority
- JP
- Japan
- Prior art keywords
- glutamine
- exchange resin
- fermentation
- ion exchange
- producing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 claims description 23
- 229930182816 L-glutamine Natural products 0.000 claims description 21
- 238000000034 method Methods 0.000 claims description 16
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims description 14
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 14
- 238000000855 fermentation Methods 0.000 claims description 13
- 230000004151 fermentation Effects 0.000 claims description 13
- 239000003456 ion exchange resin Substances 0.000 claims description 12
- 229920003303 ion-exchange polymer Polymers 0.000 claims description 12
- 229930006000 Sucrose Natural products 0.000 claims description 9
- 238000004519 manufacturing process Methods 0.000 claims description 9
- 239000005720 sucrose Substances 0.000 claims description 9
- 235000015191 beet juice Nutrition 0.000 claims description 6
- 241000894006 Bacteria Species 0.000 claims description 4
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims 2
- 239000007788 liquid Substances 0.000 description 18
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 16
- 239000002699 waste material Substances 0.000 description 13
- 239000002609 medium Substances 0.000 description 9
- 238000010612 desalination reaction Methods 0.000 description 8
- 229910052757 nitrogen Inorganic materials 0.000 description 8
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 235000000346 sugar Nutrition 0.000 description 6
- 239000000306 component Substances 0.000 description 5
- 235000013379 molasses Nutrition 0.000 description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- 241000186216 Corynebacterium Species 0.000 description 4
- CZMRCDWAGMRECN-UHFFFAOYSA-N Rohrzucker Natural products OCC1OC(CO)(OC2OC(CO)C(O)C(O)C2O)C(O)C1O CZMRCDWAGMRECN-UHFFFAOYSA-N 0.000 description 4
- 230000002378 acidificating effect Effects 0.000 description 4
- 239000003729 cation exchange resin Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 235000016068 Berberis vulgaris Nutrition 0.000 description 3
- 241000335053 Beta vulgaris Species 0.000 description 3
- 241000186146 Brevibacterium Species 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 239000003957 anion exchange resin Substances 0.000 description 3
- 229960002685 biotin Drugs 0.000 description 3
- 235000020958 biotin Nutrition 0.000 description 3
- 239000011616 biotin Substances 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- -1 hydrogen ions Chemical class 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000011347 resin Substances 0.000 description 3
- 229920005989 resin Polymers 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 108010073771 Soybean Proteins Proteins 0.000 description 2
- 241000319304 [Brevibacterium] flavum Species 0.000 description 2
- 238000005273 aeration Methods 0.000 description 2
- 150000001450 anions Chemical class 0.000 description 2
- 229940023913 cation exchange resins Drugs 0.000 description 2
- 235000014666 liquid concentrate Nutrition 0.000 description 2
- 239000003531 protein hydrolysate Substances 0.000 description 2
- 238000011218 seed culture Methods 0.000 description 2
- 235000011121 sodium hydroxide Nutrition 0.000 description 2
- 235000019710 soybean protein Nutrition 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 2
- 229960000344 thiamine hydrochloride Drugs 0.000 description 2
- 235000019190 thiamine hydrochloride Nutrition 0.000 description 2
- 239000011747 thiamine hydrochloride Substances 0.000 description 2
- 208000007848 Alcoholism Diseases 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 206010061459 Gastrointestinal ulcer Diseases 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 201000007930 alcohol dependence Diseases 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 150000003863 ammonium salts Chemical class 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000003925 brain function Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000004042 decolorization Methods 0.000 description 1
- 238000011033 desalting Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 159000000014 iron salts Chemical class 0.000 description 1
- 159000000003 magnesium salts Chemical class 0.000 description 1
- 150000002696 manganese Chemical class 0.000 description 1
- 239000012533 medium component Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000011785 micronutrient Substances 0.000 description 1
- 235000013369 micronutrients Nutrition 0.000 description 1
- 239000012452 mother liquor Substances 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 125000001477 organic nitrogen group Chemical group 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 235000021317 phosphate Nutrition 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 235000019157 thiamine Nutrition 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- 239000011721 thiamine Substances 0.000 description 1
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Description
【発明の詳細な説明】
〔発明の目的〕
（産業上の利用分野）
Ｌ−グルタミンは消化器潰瘍治療薬、アルコー
ル中毒治療薬、脳機能改善の薬剤等に用いられて
いる。本発明はこのＬ−グルタミンを発酵法で製
造する方法を改良するものである。[Detailed Description of the Invention] [Object of the Invention] (Industrial Application Field) L-glutamine is used as a drug for treating gastrointestinal ulcers, a drug for treating alcoholism, a drug for improving brain function, and the like. The present invention improves the method for producing L-glutamine by fermentation.
（従来の技術）
Ｌ−グルタミンは大量生産されているアミノ酸
であり、製造方法に種々の改良が行なわれてい
る。ビートモラセスを有機溶剤で抽出することに
よりその発酵収率を向上させる物質が得られるこ
とは知られているが（特公昭56−12111号公報）、
ビート糖製造工程の各液をイオン交換樹脂に接触
させることによりＬ−グルタミンの発酵収率を向
上させる物質が吸着されることは知られていな
い。(Prior Art) L-glutamine is an amino acid that is produced in large quantities, and various improvements have been made to the production method. It is known that a substance that improves the fermentation yield can be obtained by extracting beet molasses with an organic solvent (Japanese Patent Publication No. 12111/1983).
It is not known that a substance that improves the fermentation yield of L-glutamine is adsorbed by contacting each solution in the beet sugar manufacturing process with an ion exchange resin.
（発明が解決しようとする問題点）
Ｌ−グルタミンの製造コストを低下させるため
に発酵収率を向上させることは重要な問題であ
る。(Problems to be Solved by the Invention) Improving the fermentation yield is an important problem in order to reduce the production cost of L-glutamine.
本発明は、このような目的を達成するべくなさ
れたものであり、ブレビバクテリウム属又はコリ
ネバクテリウム属のＬ−グルタミン生産菌による
Ｌ−グルタミンの発酵培地にビート糖製造工程の
各種シユクロース含有液をイオン交換樹脂に接触
させた溶離液にＬ−グルタミンの発酵収率を向上
させる物質が含まれていることを見出したことに
基いている。
The present invention has been made to achieve such an object, and involves adding various sucrose-containing solutions used in the beet sugar production process to an L-glutamine fermentation medium using L-glutamine-producing bacteria of the genus Brevibacterium or Corynebacterium. This method is based on the discovery that the eluate obtained by contacting L-glutamine with an ion exchange resin contains a substance that improves the fermentation yield of L-glutamine.
（問題点を解決するための手段）
本発明は、ビート汁又はそれから精製糖を製造
する工程におけるシユクロース含有液をイオン交
換樹脂に接触させ、該イオン交換樹脂に吸着した
成分を溶離して、これをブレビバクテリウム属又
はコリネバクテリウム属のＬ−グルタミン生産菌
によるＬ−グルタミン発酵に用いる培地に添加す
ることを特徴とする、発酵法によるＬ−グルタミ
ンの製造法に関するものである。(Means for Solving the Problems) The present invention involves bringing beet juice or a sucrose-containing liquid in the process of producing refined sugar from it into contact with an ion exchange resin, and eluating the components adsorbed on the ion exchange resin. The present invention relates to a method for producing L-glutamine by a fermentation method, which is characterized in that it is added to a medium used for L-glutamine fermentation by L-glutamine producing bacteria of the genus Brevibacterium or Corynebacterium.
ビート汁又はそれから精製糖を製造する工程に
おけるシユクロース含有液とは、ビートを搾汁し
た原糖汁、脱色等これに種々の精製手段を施した
液、それから数段階にわたつてシユクロース結晶
を分離した母液、廃糖蜜であるビートモラセスな
どである。 Sucrose-containing liquid in the process of producing beet juice or refined sugar from it refers to raw sugar juice obtained by squeezing beets, liquid obtained by subjecting it to various purification methods such as decolorization, and liquid obtained by separating sucrose crystals from it in several steps. These include mother liquor, beet molasses, and blackstrap molasses.
ビート汁又はシユクロース含有液を接触させる
イオン交換樹脂は通常のものでよく、強酸性カチ
オン交換樹脂、中、弱酸性カチオン交換樹脂、強
塩基性アニオン交換樹脂、中、弱塩基性アニオン
交換樹脂など種々あるがそのいずれであつてもよ
い。 The ion exchange resin with which the beet juice or sucrose-containing liquid is brought into contact may be any of the usual ones, such as strongly acidic cation exchange resins, medium or weakly acidic cation exchange resins, strong basic anion exchange resins, medium or weakly basic anion exchange resins, etc. Yes, but it can be either.
本発明の方法に用いられる活性成分はビート汁
又はシユクロース含有液をイオン交換樹脂に接触
させて吸着成分を溶離することにより得られるの
であるが、現在ビート汁から精製糖を製造する工
程においてイオン交換樹脂による脱塩が行なわれ
ているのでこのイオン交換樹脂の脱塩廃液を使用
するのが簡便である。この脱塩は、冷却した粗汁
をＨ型の強酸性カチオン交換樹脂の塔に通液して
カチオンを水素イオンと交換し、塔から流出する
強酸性の糖汁をOH型の弱塩基性アニオン交換樹
脂の塔に通液してアニオンをOHイオンと交換す
ることにより行なわれる。これらのイオン交換樹
脂の再生の際に吸着された各種成分が溶離され、
この溶離液がいわゆるイオン交換樹脂塩廃液とし
て多量に副生される。この脱塩廃液は主として無
機塩類を含んでいるが、そのほか少量の有機酸
類、アミノ酸等の有機窒素を含み、糖はほとんど
含んでいない。脱塩廃液にはカチオン交換樹脂塔
からの廃液、アニオン交換樹脂塔からの廃液及び
両者の混合液があるがいずれも本発明の方法に使
用できる。これらはそのまま培地に添加してもよ
いが、適当な濃度に濃縮してから添加するほうが
一般に好都合である。培地への添加量は総窒素量
で設定すればよく、通常100mg／以上添加すれ
ば収率向上の効果が得られる。 The active ingredient used in the method of the present invention can be obtained by contacting beet juice or a sucrose-containing liquid with an ion exchange resin to elute the adsorbed components. Since desalination is carried out using a resin, it is convenient to use desalination waste liquid from this ion exchange resin. This desalting process involves passing the cooled crude juice through a tower made of H-type strongly acidic cation exchange resin to exchange cations with hydrogen ions, and converting the strongly acidic sugar juice flowing out from the tower into OH-type weakly basic anions. This is done by passing the liquid through an exchange resin tower to exchange anions with OH ions. During the regeneration of these ion exchange resins, various adsorbed components are eluted,
A large amount of this eluent is produced as a so-called ion exchange resin salt waste solution. This desalted waste liquid mainly contains inorganic salts, but also contains small amounts of organic acids, amino acids, and other organic nitrogen, and almost no sugar. The desalination waste liquid includes waste liquid from a cation exchange resin tower, a waste liquid from an anion exchange resin tower, and a mixture of the two, and any of them can be used in the method of the present invention. These may be added to the medium as they are, but it is generally more convenient to concentrate them to an appropriate concentration before adding them. The amount added to the culture medium may be determined based on the total amount of nitrogen, and usually adding 100 mg or more will improve the yield.
その他の培地成分はＬ−グルタミン発酵に用い
られる通常の培地と同様でよく、グルコース、フ
ラクトース、シユクロース等の糖類あるいはこれ
らの含有物等の炭素源、硫酸アンモニウム等のア
ンモニウム塩、アンモニア、尿素等の窒素源、リ
ン酸塩、マグネシウム塩、鉄塩、マンガン塩等の
塩類、ビオチン、サイアミン等のビタミン類、ア
ミノ酸等の有機微量栄養素等が使用される。 Other medium components may be the same as the usual medium used for L-glutamine fermentation, including carbon sources such as sugars such as glucose, fructose, and sucrose, or substances containing these, ammonium salts such as ammonium sulfate, and nitrogen such as ammonia and urea. salts such as phosphates, magnesium salts, iron salts, manganese salts, vitamins such as biotin and thiamine, organic micronutrients such as amino acids, etc. are used.
本発明の方法に使用される微生物は通常のブレ
ビバクテリウム属又はコリネバクテリウム属のＬ
−グルタミン生産菌でよく、例えば
ブレビバクテリウム・フラバム FERM−
P4272
コリネバクテリウム・アセトアシドフイラム
ATCC13870
などが使用される。 The microorganism used in the method of the present invention is a common species of the genus Brevibacterium or Corynebacterium.
-Glutamine-producing bacteria may be used, such as Brevibacterium flavum FERM-
P4272 Corynebacterium acetoacidophyllum
ATCC13870 etc. are used.
培養方法及び発酵液からのＬ−グルタミンの採
取方法は常法に従つて行なえばよく、特別な方法
を必要としない。 The culture method and the method for collecting L-glutamine from the fermentation liquid may be carried out according to conventional methods, and no special method is required.
本発明の方法は、ビート汁又はそれから精製糖
を製造する工程におけるシユクロース含有液のう
ちイオン交換樹脂吸着成分を培地に添加するだけ
でＬ−グルタミンの発酵収率を向上させることが
できる。この吸着成分には、例精製糖を製造する
工程で副生する利用価値の少ないイオン交換樹脂
脱塩廃液を有効利用できるのでその処分の負担を
軽減し、経済的に有利にＬ−グルタミンを製造す
ることができる。
The method of the present invention can improve the fermentation yield of L-glutamine by simply adding an ion-exchange resin-adsorbed component of the sucrose-containing liquid to the medium in the process of producing beet juice or refined sugar from it. For this adsorbed component, ion-exchange resin desalination waste liquid, which is produced as a by-product in the process of manufacturing refined sugar and has little utility value, can be effectively used, reducing the burden of disposal and producing L-glutamine economically. can do.
実施例 １
北海道のビート糖製糖工場の糖蜜脱塩工程で生
ずるイオン交換樹脂脱塩廃液を統窒素濃度20ｇ／
程間に濃縮した。Example 1 The ion exchange resin desalination waste liquid produced in the molasses desalination process at a beet sugar factory in Hokkaido was treated with a nitrogen concentration of 20 g/
Concentrated in time.
グリコース 100ｇ／
KH2PO4 ２〃
MgSO4・7H2O 0.5〃
（NH4）2SO4 15〃
Na2SO4 15〃
FeSO4・7H2O １mg／
ビオチン 4μｇ／
サイアミン塩酸塩 200〃
大豆蛋白加水分解液（総窒素濃度として）
0.2ｇ／
上記の組成の培地を１づつジヤーフアーメン
ターに入れ、苛性ソーダでPH7.0に調整して120℃
で10分間加熱殺菌した。これに上記の脱塩廃液濃
縮液を120℃で10分間加熱殺菌してその後述する
量を添加し、さらに予め培着しておいたブレビバ
クテリウム・フラバムFERM−P4272の種培養液
50mlを接種した。培養温度を31℃とし、アンモニ
アガスでPH6.5に保ちつつ残グルコースが無くな
るまで通気撹拌を続けた。Glyose 100g/ KH 2 PO 4 2〃 MgSO 4・7H 2 O 0.5〃 (NH 4 ) 2 SO 4 15〃 Na 2 SO 4 15〃 FeSO 4・7H 2 O 1mg / Biotin 4μg / Thiamine Hydrochloride 200〃 Soybean protein Hydrolyzate (as total nitrogen concentration)
0.2g/Pour one medium at a time with the above composition into a jar fermenter, adjust the pH to 7.0 with caustic soda, and heat at 120°C.
Heat sterilized for 10 minutes. To this, the above desalted waste liquid concentrate was heat sterilized at 120°C for 10 minutes, and then the amount described above was added, and the seed culture of Brevibacterium flavum FERM-P4272, which had been cultured in advance, was added.
50ml was inoculated. The culture temperature was set to 31°C, and while maintaining the pH at 6.5 with ammonia gas, aeration and stirring were continued until there was no remaining glucose.
得られた培養液のＬ−グルタミン濃度を常法に
より測定した対糖収率を求めた。得られた結果を
次に示す。 The L-glutamine concentration of the obtained culture solution was measured using a conventional method to determine the yield relative to sugar. The results obtained are shown below.
脱塩廃液添加量 Ｌ−グルタミン収率
（総窒素、mg／） （％）
０ 38.1
100 38.6
500 39.0
1000 39.7
2000 41.8
5000 42.5
実施例 ２
北海道のビート糖製糖工業の糖蜜脱塩工程で生
ずるイオ交換樹脂塩廃液を総窒素濃度20ｇ／程
間に濃縮した。Amount of desalination waste liquid added L-glutamine yield (total nitrogen, mg/) (%) 0 38.1 100 38.6 500 39.0 1000 39.7 2000 41.8 5000 42.5 Example 2 Io-exchange generated in the molasses desalination process of Hokkaido's beet sugar manufacturing industry The resin salt waste solution was concentrated to a total nitrogen concentration of 20 g/approx.
グリコース 100ｇ／
KH2PO4 ２ｇ／
MgSO4・7H2O 0.5ｇ／
（NH4）2SO4 15ｇ／
Na2SO4 15ｇ／
FeSO4・7H2O １mg／
ビオチン 4μｇ／
サイアミン塩酸塩 200μｇ／
大豆蛋白加水分解液（総窒素濃度として）
0.2ｇ／
上記の組成の培地を１づつジヤーフアーメン
ターに入れ、苛性ソーダでPH7.0調整して、120℃
で10分間加熱殺菌した。これに上記の脱塩廃液濃
縮液を120℃で10分間加熱殺菌してその後述する
量を添加し、さらに予め培養しておいたコリネバ
クテリウム・アセトアシドフイラム ATCC
13870の種培養液50mlを接種した。培養温度を31
℃とし、アンモニアガスでPH6.5に保ちつつ残グ
ルコースが無くなるまで通気撹拌を続けた。Glycose 100g / KH 2 PO 4 2g / MgSO 4・7H 2 O 0.5g / (NH 4 ) 2 SO 4 15g / Na 2 SO 4 15g / FeSO 4・7H 2 O 1mg / Biotin 4μg / Thiamine Hydrochloride 200μg / Soybean Protein hydrolyzate (as total nitrogen concentration)
0.2g/Pour one medium at a time with the above composition into a jar fermenter, adjust the pH to 7.0 with caustic soda, and heat at 120°C.
Heat sterilized for 10 minutes. To this, the above desalted waste liquid concentrate was heat sterilized at 120°C for 10 minutes, and the amount described above was added thereto, and further cultured in advance.
50 ml of seed culture of 13870 was inoculated. Culture temperature to 31
℃, and while maintaining the pH at 6.5 with ammonia gas, aeration and stirring were continued until there was no residual glucose.
得られた培養液をＬ−グルタミン濃度を常法に
より測定して対糖収率を求めた。得られた結果を
次に示す。 The L-glutamine concentration of the obtained culture solution was measured by a conventional method to determine the sugar yield. The results obtained are shown below.
脱塩廃液添加量 Ｌ−グルタミン収率 （総窒素、mg／） （％） ０ 32.2 1000 36.7 5000 40.5Amount of desalinated waste liquid added L-glutamine yield (Total nitrogen, mg/) (%) 0 32.2 1000 36.7 5000 40.5
Claims (1)
におけるシユクロース含有液をイオン交換樹脂に
接触させ、該イオン交換樹脂に吸着した成分を溶
離して、これをブレビバクテリウム属又はコリネ
バクテリウム属のＬ−グルタミン生産菌によるＬ
−グルタミン発酵に用いる培地に添加することを
特徴とする、発酵法によるＬ−グルタミンの製造
法。1. Contact beet juice or a sucrose-containing solution in the process of producing refined sugar from it with an ion exchange resin, elute the components adsorbed to the ion exchange resin, and extract the L- L caused by glutamine-producing bacteria
- A method for producing L-glutamine by a fermentation method, which comprises adding it to a medium used for glutamine fermentation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10173184A JPS60248195A (en) | 1984-05-22 | 1984-05-22 | Preparation of l-glutamine by fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10173184A JPS60248195A (en) | 1984-05-22 | 1984-05-22 | Preparation of l-glutamine by fermentation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS60248195A JPS60248195A (en) | 1985-12-07 |
| JPH0543356B2 true JPH0543356B2 (en) | 1993-07-01 |
Family
ID=14308413
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP10173184A Granted JPS60248195A (en) | 1984-05-22 | 1984-05-22 | Preparation of l-glutamine by fermentation |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS60248195A (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE60133304T2 (en) * | 2000-11-17 | 2009-03-05 | Cj Cheiljedang Corp. | L-GLUTAMINE-PRODUCING MICRO-ORGANISMS AND METHOD FOR THE PRODUCTION OF L-GLUTAMINE USING THE SAME |
-
1984
- 1984-05-22 JP JP10173184A patent/JPS60248195A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS60248195A (en) | 1985-12-07 |
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