JPH0552459B2 - - Google Patents
Info
- Publication number
- JPH0552459B2 JPH0552459B2 JP59061943A JP6194384A JPH0552459B2 JP H0552459 B2 JPH0552459 B2 JP H0552459B2 JP 59061943 A JP59061943 A JP 59061943A JP 6194384 A JP6194384 A JP 6194384A JP H0552459 B2 JPH0552459 B2 JP H0552459B2
- Authority
- JP
- Japan
- Prior art keywords
- sample
- electrophoresis
- leading
- tube
- ion
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000001962 electrophoresis Methods 0.000 claims description 15
- 238000002218 isotachophoresis Methods 0.000 claims description 7
- 238000013508 migration Methods 0.000 claims description 7
- 230000005012 migration Effects 0.000 claims description 7
- 239000003792 electrolyte Substances 0.000 claims description 4
- 238000002347 injection Methods 0.000 claims description 4
- 239000007924 injection Substances 0.000 claims description 4
- 150000002500 ions Chemical class 0.000 description 28
- 238000004458 analytical method Methods 0.000 description 9
- 239000007788 liquid Substances 0.000 description 6
- 238000001139 pH measurement Methods 0.000 description 4
- 229920004890 Triton X-100 Polymers 0.000 description 2
- 239000013504 Triton X-100 Substances 0.000 description 2
- UCMIRNVEIXFBKS-UHFFFAOYSA-N beta-alanine Chemical compound NCCC(O)=O UCMIRNVEIXFBKS-UHFFFAOYSA-N 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000008151 electrolyte solution Substances 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N hydrochloric acid Substances Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- UXFQFBNBSPQBJW-UHFFFAOYSA-N 2-amino-2-methylpropane-1,3-diol Chemical compound OCC(N)(C)CO UXFQFBNBSPQBJW-UHFFFAOYSA-N 0.000 description 1
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
- 229910001863 barium hydroxide Inorganic materials 0.000 description 1
- 229940000635 beta-alanine Drugs 0.000 description 1
- 238000001649 capillary isotachophoresis Methods 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000004070 electrodeposition Methods 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 230000003189 isokinetic effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/4473—Arrangements for investigating the separated zones, e.g. localising zones by electric means
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Description
【発明の詳細な説明】
(イ) 産業上の利用分野
この発明は、等速電気泳動装置に関し、特に試
料分析時における試料イオンの泳動管内の移動位
置を検出できるようにした細管式等速電気泳動装
置に関するものである。[Detailed Description of the Invention] (a) Industrial Application Field This invention relates to a constant velocity electrophoresis device, and in particular to a capillary type constant velocity electrophoresis device that can detect the movement position of sample ions in a migration tube during sample analysis. This relates to an electrophoresis device.
(ロ) 従来技術
従来の細管式等速電気泳動装置においては、試
料イオンの泳動開始から試料イオンが検出器に検
出されるまでの間は、検出器がリーデイング電解
液のイオン、すなわちリーデイングイオンを検出
するだけで、チヤート紙上に試料イオンが検出さ
れていない状態を示すベースラインが描かれるだ
けである。そのため試料イオンゾーンが検出器に
どの程度近づいているのか、どの位の時間で泳動
中の試料の分析が済むのかなどの情報が得られな
かつた。(b) Prior art In a conventional capillary type isotachophoresis device, from the start of sample ion migration until the sample ions are detected by the detector, the detector detects the ions of the leading electrolyte, that is, the leading ions. Detection simply draws a baseline on the chart paper indicating a state in which no sample ions are detected. Therefore, it was not possible to obtain information such as how close the sample ion zone was to the detector or how long it would take to complete the analysis of the sample being migrated.
そこで、試料イオンの泳動時間を予め測定する
ことによつて上記情報を得るようにしているが、
分析操作が二度手間となり作業能率が悪くなるも
のである。 Therefore, the above information is obtained by measuring the migration time of sample ions in advance.
Analyzing operations are performed twice, resulting in poor work efficiency.
(ハ) 目的
この発明は上記従来装置における分析操作の二
度手間といつた煩雑さを解消するためになされた
ものであり、試料分析者が試料分析中に泳動管内
の試料イオンゾーンの先端位置を知ることができ
るようにした等速電気泳動装置の提供を目的とす
る。(c) Purpose This invention was made in order to solve the troubles such as the double labor of analysis operations in the above-mentioned conventional apparatus, and allows a sample analyst to easily determine the tip position of the sample ion zone in the electrophoresis tube during sample analysis. The purpose of the present invention is to provide an isotachophoresis device that allows the user to know the
(ニ) 構成
この発明に係る等速電気泳動装置は、泳動管路
中に電解液のPH値を測定するためのPH測定手段
と、このPH測定手段からの出力により泳状態を表
示するための表示手段とを備えたことを特徴と
し、電解度のうちあらかじめ既知のリーデイング
液PH値を追跡することによつて、試料イオンゾー
ンの先端位置を知ることができるように構成され
ている。(d) Structure The isotachophoresis apparatus according to the present invention includes a PH measuring means for measuring the PH value of the electrolyte solution in the phoresis tube, and a means for displaying the electrophoresis state based on the output from the PH measuring means. The apparatus is characterized in that it is equipped with a display means, and is configured such that the position of the tip of the sample ion zone can be known by tracking the PH value of the leading liquid, which is known in advance among the electrolytes.
(ホ) 実施例
以下図面に基づいてこの発明の実施例である等
速電気泳動装置について説明する。(E) Embodiment An isokinetic electrophoresis apparatus which is an embodiment of the present invention will be described below based on the drawings.
第１図は細管式等速電気泳動装置の全体構成を
示す説明図である。泳動管５の一方端側に試料注
入室６を介してターミナル電極槽３と、その他方
端側に検出器７を介してリーデイング電極槽４と
がそれぞれ設置されている。試料注入室６は、リ
ーデイング液とターミナル液の境界面を形成する
とともに、試料を２液の界面に注入できる構造に
なつており、定電流電源２により両電極槽３，４
に高電圧を印加すれば、リーデイングイオン、試
料イオン、ターミナルイオンがターミナル電極槽
３側からリーデイング電極槽４側に向かつて泳動
し、移動度の差より分離され、検出器７で検出さ
れる。 FIG. 1 is an explanatory diagram showing the overall configuration of a capillary type isotachophoresis device. A terminal electrode tank 3 is installed at one end of the migration tube 5 via a sample injection chamber 6, and a leading electrode tank 4 is installed at the other end via a detector 7. The sample injection chamber 6 forms an interface between the leading liquid and the terminal liquid, and has a structure that allows the sample to be injected into the interface between the two liquids.
When a high voltage is applied to , leading ions, sample ions, and terminal ions migrate from the terminal electrode tank 3 side to the leading electrode tank 4 side, are separated based on the difference in mobility, and are detected by the detector 7 .
泳動管５の検出器７側に近い部位にはPH測定用
電極８が設置されており、泳動する電解液のPH値
を参照電極９との電位差として電位差計１０によ
り測定検出し、その測定されるPH値を表示器１１
により表示するように構成されている。 A PH measuring electrode 8 is installed in a part of the electrophoresis tube 5 near the detector 7, and the PH value of the electrolyte being electrophoresed is measured and detected as a potential difference with a reference electrode 9 by a potentiometer 10. Display 11 shows the PH value.
It is configured to be displayed by
上記のように構成されてなる細管式等速電気泳
動装置において、定電流を通電すると、分析対象
の試料イオンは、リーデイングイオンとターミナ
ルイオンに直後をはさまれた泳動が開始される。
泳動中に各イオンは易動度の違いにより、分離さ
れ、試料イオンはリーデイングイオンの後に易動
度の大きさの順に配列され、互々は界面で接する
ゾーンを形成しながらターミナルイオンを後続と
して、各イオンは等速度で泳動管５中を泳動す
る。電解液のうちリーデイング液は、そのPH値が
あらかじめわかつているので、表示器１１で表示
されるPH値を追跡することにより、PH値の変化を
読み取つてリーデイングイオンに後続する試料イ
オンゾーンの先端位置を知ることができる。 In the capillary isotachophoresis device configured as described above, when a constant current is applied, the sample ions to be analyzed start migrating with the ions immediately sandwiched between the leading ion and the terminal ion.
During electrophoresis, each ion is separated due to the difference in mobility, and the sample ions are arranged in order of mobility after the leading ion, forming a zone where they meet each other at the interface, with the terminal ion following. , each ion migrates in the migration tube 5 at a constant velocity. Among the electrolytic solutions, the PH value of the leading liquid is known in advance, so by tracking the PH value displayed on the display 11, changes in the PH value can be read and the tip of the sample ion zone following the leading ion can be detected. You can know the location.
リーデイング液のPH値は、たとえば、（0.01M
−塩酸、β−アラニン、0.2％トリトンＸ−100）
であればPH5.6、（0.01M−塩酸、アメジオール、
0.％PVA）であればPH8.8、（0.005M−MES、
0.01M−アメジオール、0.1％−HPC）であれば
PH8.56、（0.01M−水酸化バリウム、グルタミン、
0.2％トリトンＸ−100）であればPH10.0である。 For example, the PH value of the leading liquid is (0.01M
- Hydrochloric acid, β-alanine, 0.2% Triton X-100)
If PH5.6, (0.01M-hydrochloric acid, amediol,
0.%PVA), PH8.8, (0.005M−MES,
0.01M-Amediol, 0.1%-HPC)
PH8.56, (0.01M-barium hydroxide, glutamine,
0.2% Triton X-100), the pH is 10.0.
このようにPH値を測定表示させることにより、
試料イオンゾーンの先端位置がPH測定用電極８に
達したときにPH表示値が表化し、先端位置がPH測
定用電極８付近に到達したことを知ることができ
るのであるが、PH測定用電極を多数個併設し、各
電極位置でのPH値変化を読み取つて先端位置の確
認を複数個所で極細かく行なえるようにしてもよ
く、泳動状態の表示も電極の個数と同数個設ける
か、１個にして切換表示するようにすることもで
きる。さらに表示器（点滅ランプ式）を多数設け
て、これを順次点灯する方式とすることもでき
る。またPH測定用電極を易動度に応じて変化させ
ながら確認できるようにしてもよく、上記実施例
に限定されるものではない。 By measuring and displaying the PH value in this way,
When the tip position of the sample ion zone reaches the PH measurement electrode 8, the PH display value is displayed, and you can know that the tip position has reached the vicinity of the PH measurement electrode 8. It is also possible to install a large number of PH values at each electrode position so that the tip position can be checked very precisely at multiple locations. It is also possible to switch between displaying the images individually. Furthermore, it is also possible to provide a large number of indicators (flashing lamp type) and turn them on one after another. Further, the PH measurement electrode may be changed in accordance with its mobility so that confirmation can be made, and the present invention is not limited to the above embodiment.
(ヘ) 効果
この発明に係る等速電気泳動装置は上記のよう
に構成され、作動するので、試料分析中の泳動管
内の試料イオンゾーンの先端位置を容易に知るこ
とができるので、従来のように分析に先立ちあら
かじめ試料イオンの泳動時間を測定するといつた
分析操作の二度手間をなくすることができ、分析
作業能率を高めることができる。(F) Effect Since the isotachophoresis apparatus according to the present invention is constructed and operates as described above, it is possible to easily know the tip position of the sample ion zone in the electrophoresis tube during sample analysis, so that it is possible to easily determine the position of the tip of the sample ion zone in the electrophoresis tube during sample analysis. By measuring the migration time of sample ions in advance prior to analysis, it is possible to eliminate the need for double analysis operations and improve analysis work efficiency.
第１図はこの発明の１実施例である等速電気泳
動装置の構成を示す模式図である。
３……ターミナル電極槽、４……リーデイング
電極槽、５……泳動管、６……試料注入室、７…
…検出器、８……PH測定用電極、９……参照電
極、１０……電位差計、１１……表示器。
FIG. 1 is a schematic diagram showing the configuration of an isotachophoresis apparatus which is an embodiment of the present invention. 3... Terminal electrode tank, 4... Reading electrode tank, 5... Electrophoresis tube, 6... Sample injection chamber, 7...
...Detector, 8... Electrode for PH measurement, 9... Reference electrode, 10... Potentiometer, 11... Display device.
Claims (1)
ミナル電極槽を、他方端側に検出器を介してリー
デイング電極槽をそれぞれ配設し、これら両電極
槽間に高電圧を印加することによつて泳動管内に
泳動電流を通電させる等速電気泳動装置におい
て、前記泳動管路中に電解液のPH値を測定するた
めのPH測定手段と、このPH測定手段からの出力に
より試料イオンゾーンの先端位置の情報を表示す
る表示手段とを備えたことを特徴とする等速電気
泳動装置。1. A terminal electrode tank is installed at one end of the migration tube via a sample injection chamber, and a leading electrode tank is installed at the other end via a detector, and a high voltage is applied between these two electrode tanks. Therefore, in an isotachophoresis apparatus in which an electrophoresis current is passed through the electrophoresis tube, there is a PH measuring means for measuring the PH value of the electrolyte in the electrophoresis tube, and the sample ion zone is measured by the output from this PH measuring means. 1. A constant velocity electrophoresis device comprising: display means for displaying information on a tip position.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59061943A JPS60203848A (en) | 1984-03-28 | 1984-03-28 | Isokinetic electrophoresis device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59061943A JPS60203848A (en) | 1984-03-28 | 1984-03-28 | Isokinetic electrophoresis device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS60203848A JPS60203848A (en) | 1985-10-15 |
| JPH0552459B2 true JPH0552459B2 (en) | 1993-08-05 |
Family
ID=13185773
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP59061943A Granted JPS60203848A (en) | 1984-03-28 | 1984-03-28 | Isokinetic electrophoresis device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS60203848A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0573378U (en) * | 1992-03-13 | 1993-10-08 | 日信工業株式会社 | Solenoid valve device |
| JPH0573377U (en) * | 1992-03-13 | 1993-10-08 | 日信工業株式会社 | Solenoid valve device |
-
1984
- 1984-03-28 JP JP59061943A patent/JPS60203848A/en active Granted
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0573378U (en) * | 1992-03-13 | 1993-10-08 | 日信工業株式会社 | Solenoid valve device |
| JPH0573377U (en) * | 1992-03-13 | 1993-10-08 | 日信工業株式会社 | Solenoid valve device |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS60203848A (en) | 1985-10-15 |
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