JPH0565107B2 - - Google Patents
Info
- Publication number
- JPH0565107B2 JPH0565107B2 JP62209568A JP20956887A JPH0565107B2 JP H0565107 B2 JPH0565107 B2 JP H0565107B2 JP 62209568 A JP62209568 A JP 62209568A JP 20956887 A JP20956887 A JP 20956887A JP H0565107 B2 JPH0565107 B2 JP H0565107B2
- Authority
- JP
- Japan
- Prior art keywords
- adhesive tape
- skin
- cells
- cell sample
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
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- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Description
【発明の詳細な説明】
（産業上の利用分野）
本発明は、皮膚細胞サンプル作成法に関し、特
に、皮膚の状態を調べるために、人体から皮膚細
胞を剥離しプレパラート上に載せ顕微鏡で検査す
る際に用いるのに適した皮膚細胞のサンプル（標
本）を作成する方法に関するものである。[Detailed Description of the Invention] (Industrial Application Field) The present invention relates to a method for preparing a skin cell sample, and in particular, in order to investigate the condition of the skin, skin cells are peeled off from a human body, placed on a slide, and examined under a microscope. This invention relates to a method for preparing skin cell samples (specimens) suitable for use in clinical trials.
（従来の技術）
従来の皮膚細胞サンプルを作成する方法を第２
ａ図〜第２ｆ図を参照して説明すると、第２ａ図
に示すように、細胞を採取しようとする、例えば
人体の腕１０に底が空いているガラスカツプ３０
を置き、界面活性剤、例えば0.1％ TRITON
Ｘ−100（りん酸バツフアーPH7.2）の２mlをスポ
イト３６でガラスカツプ３０の上部開口から注入
する。次に、第２ｂ図に示すように、１分間ガラ
ス棒２８で皮膚表面を擦り、皮膚細胞を溶液中に
採り込む。次に、第２ｃ図に示すように、皮膚細
胞が縣濁した溶液の0.2mlをスポイト３８で容器
３２に移す。次に、第２ｄ図に示すように、この
ようにして得た容器３２内の試料にスポイト４０
で染料、例えばメチレンブルーとローダミンとの
１対１の混合液（飽和水溶液）を１滴滴下して皮
膚細胞を染色する。次に、第２ｅ図に示すよう
に、染色した皮膚細胞を含む溶液数滴をスポイト
４２でプレパラート２０上に移し、最後に第２ｆ
図に示すように、プレパラート２０上の試料を覆
うようにカバーグラス２４を載せて顕微鏡で観察
するための皮膚細胞サンプルを作成する。(Conventional technology) The conventional method for creating skin cell samples is
To explain with reference to Figures a to 2f, as shown in Figure 2a, a glass cup 30 with an open bottom, for example, a human arm 10 from which cells are to be collected, is used.
Place a surfactant, e.g. 0.1% TRITON
Inject 2 ml of X-100 (phosphoric acid buffer pH 7.2) from the upper opening of the glass cup 30 using the dropper 36. Next, as shown in Figure 2b, the skin surface is rubbed with a glass rod 28 for one minute to incorporate the skin cells into the solution. Next, as shown in FIG. 2c, 0.2 ml of the suspension of skin cells is transferred to a container 32 using a dropper 38. Next, as shown in FIG. 2d, the sample in the container 32 obtained in this way is placed using a dropper 40.
Then, one drop of a dye such as a 1:1 mixture of methylene blue and rhodamine (saturated aqueous solution) is added to stain the skin cells. Next, as shown in FIG. 2e, several drops of the solution containing the stained skin cells are transferred onto the preparation 20 using the dropper 42, and finally
As shown in the figure, a cover glass 24 is placed to cover the sample on the preparation 20 to prepare a skin cell sample for observation under a microscope.
（発明が解決しようとする問題点）
前述したように、従来の皮膚細胞サンプル作成
法は、各種の器具を用いなければならず、またサ
ンプル作成までに多数のステツプを要し、長時間
を要する。また、細胞採取個所が顔、背中等では
顔を傾けたり、寝そべる姿勢をとる必要があり、
面倒である。(Problems to be Solved by the Invention) As mentioned above, the conventional skin cell sample preparation method requires the use of various instruments, requires many steps to prepare the sample, and takes a long time. . In addition, if the cell collection site is your face or back, you may need to tilt your head or lie down.
It's a hassle.
さらにまた、顔面では、採取する細胞数が少な
く、皮膚の状態を調べるのに必要な数である通常
約30個を得るためには多数のサンプルを作成しな
ければならない。 Furthermore, the number of cells collected from the face is small, and a large number of samples must be made to obtain the number needed to examine the condition of the skin, usually about 30.
したがつて、本発明の目的は、少ない器具によ
り少ないステツプで短時間に皮膚細胞サンプルを
作成する方法を提供することにある。 Therefore, an object of the present invention is to provide a method for preparing a skin cell sample in a short time using fewer instruments and fewer steps.
また、本発明の他の目的は、細胞採取時に液体
を用いず人体のどの個所からも用意に細胞を採取
でき、しかも顕微鏡による観察に適した重なりが
少ない充分に角質の細胞を得ることができる皮膚
細胞サンプル作成法を提供することにある。 Another object of the present invention is to be able to easily collect cells from any part of the human body without using liquid during cell collection, and to obtain sufficiently horny cells with few overlaps suitable for microscopic observation. The purpose of the present invention is to provide a method for preparing skin cell samples.
（問題点を解決するための手段）
前述の目的を達成するために、本発明は、第１
の接着テープを皮膚に押し付けて皮膚細胞を第１
の接着テープ上に付着させ、第２の接着テープを
貼付けたプレパラートを用意し、第１の接着テー
プをプレパラートの第２の接着テープに当接させ
て第１の接着テープに付着した皮膚細胞を第２の
接着テープに転写する、ことを特徴とする皮膚細
胞サンプル作成法を採用するものである。(Means for solving the problems) In order to achieve the above-mentioned object, the present invention provides the first
Press the adhesive tape onto the skin to remove the skin cells first.
Prepare a preparation on which a second adhesive tape is attached, and bring the first adhesive tape into contact with the second adhesive tape of the preparation to remove the skin cells attached to the first adhesive tape. This method employs a skin cell sample preparation method characterized by transferring the sample to a second adhesive tape.
（実施例） 本発明を図面を参照して説明する。(Example) The present invention will be explained with reference to the drawings.
第１ａ図〜第１ｃ図は本発明の皮膚細胞サンプ
ル作成法の各ステツプを説明するための概略斜視
図である。 Figures 1a to 1c are schematic perspective views for explaining each step of the skin cell sample preparation method of the present invention.
第１ａ図に示すように、片面接着の紙テープで
あることが好ましい接着テープ（第１の接着テー
プ）１４のロールを内蔵しており、その接着テー
プ１４の接着側部を開口で露呈させて接着テープ
を送るように構成した採取器具１２を用いて、例
えば腕１０の採取個所に接着テープ１４を当接さ
せ、皮膚細胞を腕から剥離させて接着テープ１４
に付着させる。 As shown in FIG. 1a, a roll of adhesive tape (first adhesive tape) 14, which is preferably a one-sided adhesive paper tape, is built in, and the adhesive side of the adhesive tape 14 is exposed through an opening for bonding. For example, the adhesive tape 14 is brought into contact with the collection site of the arm 10 using the collection device 12 configured to feed the tape, and the skin cells are peeled off from the arm and the adhesive tape 14 is removed.
attach it to.
次に、第１ｂ図に示すように、透明の両面接着
テープ２２を貼付けたプレパラート２０を用意
し、その接着テープ２２に採取器具１２の接着テ
ープ１４を当接させて接着テープ１４に付着した
角質細胞を転写する。この転写により、接着テー
プ１４に層状になつて付着していた角質細胞の内
表面の角質細胞だけが接着テープ２２に付着する
ことになり、顕微鏡による観察の際の望ましくな
い重なりが避けられ、観察に適した細胞の数と分
布がえられる。 Next, as shown in FIG. 1b, a preparation 20 with a transparent double-sided adhesive tape 22 pasted thereon is prepared, and the adhesive tape 14 of the sampling device 12 is brought into contact with the adhesive tape 22 to remove dead skin cells attached to the adhesive tape 14. Transcribe the cells. Through this transfer, only the inner surface corneocytes of the corneocytes that had been attached to the adhesive tape 14 in a layered manner are attached to the adhesive tape 22, thereby avoiding undesirable overlap during microscopic observation. The appropriate number and distribution of cells can be obtained.
次に、第１ｃ図に示すように、スポイト２６で
界面活性剤と染料との混合液、例えばTRITON
Ｘ−100とメチレンブルーとの混合液を接着テー
プ２２上に滴下し、皮膚細胞を着色する。 Next, as shown in FIG. 1c, a mixture of surfactant and dye, such as TRITON, is added using a dropper 26.
A mixture of X-100 and methylene blue is dropped onto the adhesive tape 22 to color the skin cells.
次に、自然乾燥或いはドライヤーなどで強制的
に乾燥させサンプルを完成させる。 Next, the sample is completed by drying naturally or forcibly using a dryer.
（発明の効果）
前述したように、本発明は、接着テープを内蔵
した採取器具を用いて接着テープにより人体から
皮膚細胞を剥離し、プレパラート上の接着テープ
に転写し、着色するので、サンプルを短時間で作
成でき、細胞採取時に液体を用いないため、人体
のどの個所からも容易に観察に適した角質細胞を
えることができる。(Effects of the Invention) As described above, the present invention uses a collecting device with a built-in adhesive tape to peel off skin cells from the human body with the adhesive tape, transfer the skin cells to the adhesive tape on the preparation plate, and color them. Since it can be created in a short time and no liquid is used when collecting cells, corneocytes suitable for observation can be easily obtained from any part of the human body.
第１ａ図〜第１ｃ図は本発明の皮膚細胞サンプ
ル作成法の各ステツプを説明するための概略斜視
図である。第２ａ図〜第２ｆ図は従来技術の皮膚
細胞サンプル作成法の各ステツプを説明するため
の概略斜視図である。
１０……腕、１２……採取器具、１４……接着
テープ、２０……プレパラート、２２……接着テ
ープ、２４……カバーグラス。
Figures 1a to 1c are schematic perspective views for explaining each step of the skin cell sample preparation method of the present invention. Figures 2a to 2f are schematic perspective views for explaining each step of a conventional skin cell sample preparation method. 10... Arm, 12... Collection device, 14... Adhesive tape, 20... Preparation, 22... Adhesive tape, 24... Cover glass.
Claims (1)
胞を第１の接着テープ上に付着させ、 第２の接着テープを貼付けたプレパラートを用
意し、 第１の接着テープをプレパラートの第２の接着
テープに当接させて第１の接着テープに付着した
皮膚細胞を第２の接着テープに転写する、 ことを特徴とする皮膚細胞サンプル作成法。 ２ 皮膚細胞を第２の接着テープに転写後、皮膚
細胞を着色する、ことを特徴とする特許請求の範
囲第１項に記載の皮膚細胞サンプル作成法。 ３ 前記皮膚細胞の着色が界面活性剤と染料との
混合液による染色によつて行われることを特徴と
する特許請求の範囲第２項に記載の皮膚細胞サン
プル作成法。 ４ 前記第１の接着テープが片面接着の紙テープ
であることを特徴とする特許請求の範囲第１項に
記載の皮膚細胞サンプル作成法。 ５ 前記第２の接着テープが着色しにくい透明の
両面接着テープであることを特徴とする特許請求
の範囲第１項に記載の皮膚細胞サンプル作成法。[Claims] 1. Press a first adhesive tape against the skin to cause skin cells to adhere to the first adhesive tape, prepare a preparation to which a second adhesive tape is attached, and press the first adhesive tape to the preparation. A method for preparing a skin cell sample, comprising: transferring skin cells adhered to a first adhesive tape to the second adhesive tape by bringing the skin cells into contact with the second adhesive tape. 2. The skin cell sample preparation method according to claim 1, wherein the skin cells are colored after being transferred to the second adhesive tape. 3. The skin cell sample preparation method according to claim 2, wherein the skin cells are colored by staining with a mixture of a surfactant and a dye. 4. The skin cell sample preparation method according to claim 1, wherein the first adhesive tape is a paper tape with one-sided adhesive. 5. The skin cell sample preparation method according to claim 1, wherein the second adhesive tape is a transparent double-sided adhesive tape that does not easily stain.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62209568A JPS6453165A (en) | 1987-08-24 | 1987-08-24 | Preparation of cutaneous cell sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62209568A JPS6453165A (en) | 1987-08-24 | 1987-08-24 | Preparation of cutaneous cell sample |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6453165A JPS6453165A (en) | 1989-03-01 |
| JPH0565107B2 true JPH0565107B2 (en) | 1993-09-17 |
Family
ID=16574986
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62209568A Granted JPS6453165A (en) | 1987-08-24 | 1987-08-24 | Preparation of cutaneous cell sample |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6453165A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006522337A (en) * | 2003-03-31 | 2006-09-28 | サイティック コーポレイション | Papanicolaou staining |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU5575294A (en) * | 1992-12-07 | 1994-07-04 | Hisamitsu Pharmaceutical Co., Inc. | Plaster for testing and method of testing |
| JPH09308619A (en) * | 1996-05-23 | 1997-12-02 | Kao Corp | Method and device for skin surface analysis |
| WO2013105363A1 (en) * | 2012-01-13 | 2013-07-18 | ポーラ化成工業株式会社 | Cell encapsulation method, and cell observation method |
| CN112577794A (en) * | 2019-09-29 | 2021-03-30 | 广东体必康生物科技有限公司 | Method for preparing film-taking enriched bacteria by shadowless adhesive and application thereof |
-
1987
- 1987-08-24 JP JP62209568A patent/JPS6453165A/en active Granted
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006522337A (en) * | 2003-03-31 | 2006-09-28 | サイティック コーポレイション | Papanicolaou staining |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6453165A (en) | 1989-03-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| EXPY | Cancellation because of completion of term |