JPH0578309B2 - - Google Patents

Description

[Detailed description of the invention]

1 Technology/Field The present invention relates to Rhodococcus erythropores (former name: Nocardia erythropores) KR-S-
As represented by 1 share (FERM P3530),
A culture obtained by culturing Rhodococcus bacteria capable of producing a flocculant or a culture-treated product is brought into contact with a fermentation residue suspension in the coexistence of a cationic substance or under acidic conditions to flocculate it, and the resulting product is useful. It concerns a method for recovering materials. 2. Prior Art Fermentation residue suspension is said to be one of the waste fluids that is difficult to process afterwards due to its high BOD (biochemical oxygen demand). However, since the residue suspension contains an extremely rich nutrient source, its value as feed, for example, has recently been reconsidered. Since ancient times in Japan, sake brewers have adopted a squeezing process for clarification after fermentation, resulting in the formation of highly nutritious sake lees as a by-product, some of which is eaten as food or as a pickle to enrich the diet. There is. In addition to alcoholic beverages such as sake and beer, which are made from the fermented liquid itself, there are also distilled spirits such as whiskey and shochiyu. Even in these fermented spirits, the residue suspension of the distillate contains extremely rich resources with high nutritional value. Although some of the residue suspension after distillation is used as feed by thermal concentration, most of it is used due to the safety of the processed material using conventional flocculants, the complexity of PH adjustment during processing, and the cost. It is left untouched and has been treated with activated sludge, dumped in forests, or dumped into the ocean. Under these circumstances, in Japan, which is a major feed importer, if we can aggregate and collect valuable useful ingredients for feed, etc. from the fermented residue suspension and effectively use them as feed, we can simultaneously treat BOD removal from high BOD wastewater. Resources will be reused and recycled, and the social benefits are thought to be extremely large. 3. Problems to be solved by the invention Based on this background, useful substances that can be safely reused as feed can be processed from fermentation residue suspensions, etc. in a simple and safe manner without the risk of secondary pollution. It's about finding a way. 4 Means for solving the problem Microorganism-produced flocculant developed by the present inventors
It was discovered that NOC-1 (Patent No. 1096062) has excellent effects, and the present invention was completed. That is, according to the present invention, a culture and/or a culture-treated product obtained by culturing a microorganism belonging to the genus Rhodococchus and having a flocculating ability is treated with a fermentation residue liquid, etc. in the presence of a cationic substance or under acidic conditions. Provided is a method for flocculating and recovering a suspension of alcoholic fermentation residues using a microorganism-produced flocculant, which comprises bringing the suspension into contact with a microorganism-produced flocculant. The strain used in the present invention may be any strain that belongs to the genus Rhodococcus and has agglutinating ability, but a representative example of the strain is Rhodococcus erythropores (formerly known as Nocardia erythropores) KR-S-1 (FERM P3530). No.) has been deposited. The former name Nocardia erythropores was reorganized and reorganized into Rhodococcus erythropores by the International Committee on Nomenclature of Microorganisms in 1980.
It has been reclassified. Culture media for such strains include glucose,
Carbon sources such as fructose, inorganic nitrogen sources such as urea and ammonium sulfate, organic nitrogen sources such as yeast extract, and other nutritional sources such as inorganic salts and vitamins are used. Culture may be liquid culture or solid culture. It is usually carried out using aerated agitation liquid culture. Initial pH of culture is PH4 ~
11, carried out at a temperature range of 20-40℃. The culture can be completed in about 2 days to 1 week, and a culture having flocculation ability can be obtained. From the supernatant liquid from which bacterial strains have been removed by centrifugation or the like, aggregated substances can be separated by ammonium sulfate precipitation or the like, and the cultured product can be recovered. In the present invention, there is no need to use the culture product purified in this way, and the culture itself may be used, and since the bacterial cells themselves have aggregation ability, they can be used as they are. The fact that the microorganisms of the genus Cordococcus used in the present invention is non-pathogenic is described in Virgie Manual Systematic Bacteriology Vol. 2,
(Bergey's Manual of Systematic
Bacteriology Vol. 2), pp. 1472-1478, and Agricultural Biological Chemistry 41
Vol., (Agric.Biol.Chem.Vol.41), pages 1031-1038. Therefore, the useful substances coagulated and recovered in the present invention fully satisfy the specifications of the Feed Safety Act. In the present invention, the culture and/or culture-treated product obtained as described above is brought into contact with a fermentation residue suspension or the like to aggregate useful resources in the residue, but in this case, cations By adding a system substance or by contacting under acidic conditions,
More effective coagulation recovery can be performed.
The cationic substance is preferably a compound that generates a polyvalent cation of divalent or higher valence, and for example, calcium chloride, aluminum chloride, magnesium chloride, manganese chloride, etc. are advantageously used. In the method of the present invention, generally, the culture and/or culture treatment product used in the method of the present invention is added to the fermentation residue suspension, and then a cationic substance is added, or preferably under acidic conditions. This is carried out by reducing the pH to 5 or lower, but the method of carrying out is not particularly limited. The aggregates thus obtained can be used as highly nutritious feed, etc. Examples where the fermentation residue suspension that is the object of the present invention is produced include alcohol fermentation (manufacturing of industrial alcohol, whisky, beer, sake, sake, etc.);
Amino acid fermentation (glutamic acid, serine, seasoning,
(Production of methionine, isoleucine, etc.), antibiotic fermentation (Production of penicillin, streptomycin, kanamycin, etc.), Nucleic acid fermentation (Production of inosinic acid, etc.), Enzyme fermentation (Production of amylase, protease, lipase, etc.), Organic acid fermentation (Production of lactic acid, etc.) , production of citric acid, etc.). 5 Examples Next, the present invention will be explained in more detail using examples. Note that SS (suspended solids), COD (chemical oxygen demand), and SV ₃₀ (sludge volume index after standing still for 30 minutes) were all measured according to JIS standards. Further, the microorganism-produced flocculant NOC-1 used in the following examples was obtained as follows. Glucose 5g, Fructose 5g, KH ₂ PO ₄
2g, K ₂ HPO ₄ 5g, MgSO ₄ 0.2g, urea 0.5g,
Dissolve 0.5g of yeast extract in 1 part of distilled water and add to the culture medium.
Transfer 100 ml of the medium adjusted to pH 8.0 into a 300 ml Erlenmeyer flask, sterilize it by autoclaving at 120℃ for 15 minutes, and then transform it into Rhodococcus erythropores (former name: Nocardia erythropores).
One loopful of KR-S-1 strain (FERM P3530) is transplanted into a flask. Rotary culture was performed at 30°C for 4 to 5 days to obtain a culture. Cells were removed from this culture by cooling centrifugation, and ammonium sulfate was added to the centrifuged supernatant to give a saturation of 0.8. The ammonium sulfate precipitate is collected using a dialysis membrane (Visking).
Dialysis is performed in a tube), and then the cultured product, which is a sticky product, can be obtained by freeze-drying the dialyzed fluid. This cultured product is redissolved in distilled water or the like before use. [Example 1] 400 ml of whiskey fermentation distillation residue liquid was placed in a 500 ml beaker, and the above culture was brought to the final concentration using a stirrer equipped with a blade plate of 20 mm x 40 mm at 150 revolutions per minute.
5000ppm or culture treated product at final concentration 500ppm
After addition, the pH was adjusted to 3.9 with acetic acid (or hydrochloric acid), and stirring was continued for an additional 5 minutes. After allowing the reaction solution to stand still for 30 minutes, the SV ₃₀ of the reaction solution
And the COD and SS of the supernatant were measured. For comparison, cultured products (or cultured products)
A control group to which no chloride was added was measured according to the method described above. The results are shown in Table 1.

[Table] By bringing NOC-1 (culture or culture-treated product) into contact with the whiskey fermentation and distillation residue under acidic conditions, useful substances in the whiskey fermentation and distillation residue can be effectively recovered. found. A portion of such a whiskey fermentation residue suspension is currently concentrated by thermal concentration and turned into feed. By using the present flocculant in this concentration step, it is expected that energy consumption will be significantly reduced, which can also be expected to contribute to cost reduction. [Example 2] The lees after the sake squeezing process were thoroughly ground in a mortar, suspended in distilled water to make a 1w/v% liquid, and then further suspended uniformly in a homogenizer to complete the sake squeezing process. A similar solution was prepared before. NOC-1 (culture solution) 2 in a 100ml graduated cylinder
After taking 1 ml and making it to 10 ml with distilled water, add 80 ml of this squeezed lees adjustment suspension, further add 10 ml of distilled water, adjust the pH to 3.9 to 4.0 with acetic acid, and let it stand for 30 minutes. The SS of the supernatant liquid was measured. Note that in the control group, distilled water was used instead of 2 ml of the culture solution. The results are shown in Table 2.

[Table] NOC in the adjusted suspension of fermented rice lees
It was found that by adding -1 (culture) and setting the pH at the time of aggregation to acidic conditions, a fine suspension of squeezed lees could be coagulated and precipitated and recovered. [Example 3] Is it possible to collect the flocculation and sedimentation of the suspension by changing the conditions during the flocculation reaction of the squeezed lees-adjusted suspension obtained in Example 2 to the coexistence of a cationic substance instead of under acidic conditions? Please consider it. NOC-1 (culture solution) in a 100ml graduated cylinder
After adding 2 ml and increasing the volume to 10 ml with distilled water, then adding 80 ml of the squeezed lees adjustment suspension,
Add 10 ml of calcium chloride solution ( ₂ solutions of 10% CaCl), mix well, and adjust the pH of the mixture to 6.9 to 7.0. 30
The COD and SS of the supernatant liquid after standing for minutes were measured, and the recovery of the adjusted suspension was observed. The results are shown in Table 3. As shown in Table 3, by contacting in the presence of a cationic substance, the NOC-1 of the prepared suspension was
It was found that coagulation recovery is possible by

[Table] [Example 4] Fermented bulk, which is Shochiyu production lees, at 10v/
It was adjusted to be v%. After thoroughly dissolving 0.2 mg of the cultured product in 10 ml of distilled water, add 80 ml of Shochiyu fermentation bulk liquid.
Furthermore, 10 ml of calcium chloride solution ( ₂ solutions of 10% CaCl) was added to adjust the pH at the time of aggregation to 6.9 to 7.0. As a control, distilled water without culture treatment was used.
A similar operation was performed using 10 ml to serve as a control. Supernatant liquid SS and
COD was measured. The results are shown in Table 4.

[Table] It has been shown that fermentation distillation lees water can be coagulated and recovered by allowing this flocculant to act on fermented distillation lees (fermented bulk) in the presence of cationic substances. 6 Effects As shown by the results shown above, fermentation residue suspension can be improved by bringing this flocculant into contact with the fermentation residue suspension under acidic conditions, preferably at a pH of 5 or below, or in the presence of cationic substances. Useful substances (feed, etc.) by simple treatment of turbid liquid and collection of flocculation and sedimentation at the same time.
It has become clear that safe reuse of the materials can be expected.

Claims (1)

[Claims] 1. A flocculant containing a Rhodococcus bacterial culture and/or a processed product thereof as a main component is brought into contact with a fermentation residue suspension in the presence of a cationic substance to flocculate useful substances. How to collect. 2. A method for aggregating and recovering useful substances by bringing the culture and/or culture-treated product according to claim 1 into contact with a fermentation residue suspension under acidic conditions.