JPH058880Y2 - - Google Patents

Description

[Detailed description of the invention] [Industrial application field] This invention is a biological system that is used in biological reaction experiments such as the production of amino acids and alcohol, and the purification of organic wastewater, which requires enzymes and microorganisms. This invention relates to a reaction separation experimental device.

[Prior Art] Conventionally, a biological reaction separation device (MB: Membrane
bioreactor). This MB is
It is equipped with a reaction device for obtaining reactions by enzymes and microorganisms, and a separation device for extracting only the reaction treatment liquid without leaking enzymes and microorganisms.
In addition, the separation device is equipped with an ultrafiltration membrane,
The concentrated liquid that accumulates on the upstream side of the membrane is returned to the reaction device through a reflux device.

On the other hand, when performing the treatment using MB as described above, it is necessary to conduct various experiments prior to implementation, for example, in order to confirm and research the performance of the membrane, the reaction state, etc. Conventionally, such experiments
It is customary to use experimental equipment that is a scaled-down version of the actual machine.

[Problems that the invention aims to solve] However, a scaled-down experimental device necessarily includes a reaction device, a separation device, and a reflux device, which makes the device complex and large for an experimental device. This not only increases the cost of the device, but also requires the preparation of a large amount of samples, which is a major obstacle to easy experiments.

This idea was made in view of the above-mentioned current situation.
The purpose of this invention is to provide a simple biological reaction experiment device that enables easy experiments.

[Means to solve the problem] The measures taken to achieve the above objectives are as follows:
To explain with reference to FIG. 1, which corresponds to an embodiment of the present invention, grooves 3a and 3b communicating with the outflow pipe 2 are formed in the bottom surface of the reaction vessel 1, and a membrane 4 is formed by covering the grooves 3a and 3b. The above object has been achieved by providing a biological reaction separation experimental apparatus which is equipped with a stirring blade 5 which is horizontally rotated above the membrane 4 and close to the filter membrane 4.

[Function] According to the present invention, the reaction vessel 1 functions as both a reaction device and a separation device, and a reflux device can be omitted. That is, the reaction vessel 1 above the membrane 4
The sample is subjected to reaction in the inner space, and the space is also connected to the outflow pipe 2 via the membrane 4 and the grooves 3a and 3b.
Since it is connected to, for example, by suctioning from the outflow pipe 2, only the reaction treatment liquid can be directly taken out from inside the reaction vessel 1. Further, by taking out the reaction treatment liquid, a concentrated liquid is left in the space inside the reaction vessel 1 above the membrane 4, but since this space itself is a reaction field, the concentrated liquid is returned to the reaction apparatus. There is no need for a reflux device, and experiments can be continued by adding samples. and,
One reaction vessel 1 has the function of integrating both a reaction device and a separation device, and has a simple structure without a reflux device, so the device can be made small and inexpensive. If the reaction device and the separation device are integrated and the reflux device is omitted, the solid content concentration on the surface of the membrane 4 will increase as the reaction treatment liquid is taken out, and the permeability of the membrane 4 will be lost in a short time. It becomes impossible to carry out continuous experiments for a long time. Therefore, in the present invention, a stirring blade 5 is provided. This stirring blade 5 generates turbulent flow on the surface side of the membrane 4,
This prevents the generation of a slime layer that causes the membrane 4 to lose its permeability. Therefore, in the present invention, the good permeation ability of the membrane 4 is maintained for a long time even though the reflux device is omitted.

[Example] As shown in FIGS. 1 and 2, the reaction container 1 includes a lower container 1a and an upper container 1b packed together by a packing 6a.
The screws 7 and thumbscrews 8 are used to fit the screws 7 and 8 into place. This lower container 1a and upper container 1
b are both made of a transparent material such as synthetic resin or glass, so that the inside can be observed.

Four concentric circular grooves 3a are formed on the bottom surface of the reaction container 1, that is, the inner bottom surface of the lower container 1a. An outflow pipe 2 is provided penetrating the side wall of the lower container 1a, and each of the circular grooves 3a is communicated with the outflow pipe 2 via a straight groove 3b formed in the radial direction. And these grooves 3a, 3b
A membrane 4 is arranged to cover the membrane 4.
A packing 6a is located on the peripheral edge of the upper container 1b, and the packing 6a is further pressed down from above by the upper container 1b.
The lower edge of is fitted into the lower container 1a.

A hollow cylindrical projection 9 projects upward from the center of the upper container 1a, and an inflow pipe 10 communicating with the interior space of the reaction container 1 above the membrane 4 is connected to the upper container 1.
It protrudes from the upper surface of b. The inflow pipe 10 is for supplying a sample into the reaction vessel 1. Furthermore, inside the protrusion 9, an air passage 11 that penetrates vertically is provided.
A support shaft 12 having an air hole 13 is attached to the upper end of the protrusion 9.
are screwed together via packing 6b. Air flowing in from the air hole 13 of the lid body 14 flows through the air path 11.
It enters the reaction vessel 1 from the inside and assists in the aerobic reaction within the reaction vessel 1. When performing an anaerobic reaction within the reaction vessel 1, the air hole 13 may be closed.

A rotating shaft 15 is loosely inserted through the center of the supporting shaft portion 12, and a nut 16 is screwed onto the upper end of the rotating shaft 15, so that the rotating shaft 15 can freely rotate with respect to the supporting shaft portion 12. It is in a suspended state. A stirring blade 5 is attached to the lower end of the rotating shaft 15, and is adapted to be rotated close to and above the membrane 4.

The stirring blade 5 is a magnet coated with synthetic resin, and is rotated horizontally from the outside of the reaction vessel 1 by a magnetic device 17. As the magnetic device 17, a commercially available magnetic stirrer or the like can be used.

In particular, as in this embodiment, if the stirring blade 5 is magnetized and rotated by the magnetic device 17 from outside the reaction vessel 1, a motor for rotating the stirring blade 5 in the reaction vessel 1 can be used. There is no need to install etc. Therefore, it is possible to prevent an increase in the size of the reaction vessel 1 due to the attachment of a motor or the like, and to prevent adverse effects on the reaction due to heat generated due to rotation of the motor.

Another embodiment will be described with reference to FIG. 3. In FIG. 3, the upper container 1b has a vertically long cylindrical shape. A support plate 19 having an opening 18 is attached to the lower part of the upper container 1b.
A support shaft portion 12 is attached to the center of 9. still,
In FIG. 3, members with the same reference numerals as in FIGS. 1 and 2 indicate the same members as in the previous embodiment.

By making the upper container 1b into a long cylindrical shape in this way, a large amount of sample can be subjected to the reaction at once, and the water head pressure can be used when taking it out from the outflow pipe 2, which allows the reaction treatment liquid to permeate through the membrane 4. becomes easier.

[Effects of the invention] According to the invention, it becomes possible to conduct experiments easily regardless of the size of the sample using a simple and inexpensive device, making it easier to conduct research on MB.

[Brief explanation of the drawing]

FIG. 1 shows the second embodiment of the device according to an embodiment of the present invention.
FIG. 2 is a plan view of the lower container, and FIG. 3 is a longitudinal sectional view of another embodiment. 1...Reaction container, 1a...Lower container, 1b...Upper container, 2...Outflow pipe, 3a, 3b...Groove, 4...
Membrane, 5... Stirring blade, 6a, 6b... Packing, 7... Screw, 8... Thumb screw, 9... Projection, 10... Inflow pipe, 11... Ventilation path, 12...
Support shaft portion, 13... Air hole, 14... Lid body, 15...
...rotating shaft, 16...nut, 17...magnetic device.

Claims (1)

[Scope of utility model registration request] A groove that communicates with the outflow pipe is formed in the bottom of the reaction vessel, a membrane is placed over the groove, and a stirring blade that is rotated horizontally above the membrane is provided close to the membrane. A biological reaction separation experimental device featuring: