JPH0617918B2 - Automatic analyzer - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION (a) Field of Industrial Application The present invention relates to an automatic analyzer, which is used for preparing a reagent blank solution and for analyzing the concentration of a test component in a sample from its measured value.

(B) Conventional technology When analyzing a sample (specimen) using an automatic analyzer, deionized water is taken instead of the specimen prior to the sample analysis, and an analytical reagent is added to it to measure the wavelength of light. It was necessary to confirm that the measured absorbance value (reagent blank value) showed an acceptable constant value. This is because the concentration of the test component was obtained by multiplying the value obtained by subtracting the reagent blank value from the absorbance of the reaction solution obtained by injecting the reagent into the sample by the concentration conversion coefficient.

However, with this analysis method, if each laboratory analyzes about 50 to 500 samples in a routine test at a nearly fixed time during the daytime, the reagent blank value should be measured at least several times, usually 10 times. However, the utilization rate of analytical reagents for actual sample analysis is 80-98% [(50-10) 100/50
%, (500-10) · 100/500%), and assuming that 1 to 5 samples are analyzed after routine or during an emergency analysis at night, the utilization rate of the analytical reagent in this case is 10 to 50%
(1/10 × 100%, 5/10 × 100%), which is significantly lower.

Therefore, in the conventional device, regardless of whether or not it is a routine test, expensive analytical reagents are wastefully lost,
In addition, it takes a long time to prepare the reagent blank solution, so that the analysis cannot be performed economically and efficiently.

(C) Purpose The present invention has been made in view of the above circumstances, and one of the main objects thereof is to reduce the number of times of measurement of a reagent blank solution to increase the utilization rate of an expensive analysis reagent and to provide a reagent blank solution. It is an object of the present invention to provide an automatic analyzer capable of economically and efficiently performing analysis by reducing the time required for preparation of.

(D) Structure The present invention dispenses the same amount of distilled water and a reagent instead of the sample into every other one of a large number of reaction vessels which are sequentially arranged and transported by the transporting means, and the remaining reaction vessels have the sample and the reagent. Dispensing means for dispensing and, provided in the moving path of each reaction container, the reaction liquid generated by the sample and the reagent in each reaction container for the predetermined absorption wavelength, and the same amount of distilled water and the reagent instead of the sample The measuring means for respectively measuring the absorbance and the change in the absorbance of the mixed solution, the storage means for storing the output signal output from the measuring means, and the output before and after the measurement of each reaction solution based on the storage signal output from the storage means. Calculation display means for calculating an average value of two mixed liquid measurement signals, and subtracting the average from each reaction liquid measurement signal output during the measurement of the mixed liquid to calculate and display the concentration of the test component in the sample. , And And an automatic analyzer equipped with control means for controlling the operation of each of the above means, and a plurality of reaction vessels alternately arranged in sequence by the transport means and instead of the sample, the same amount of distilled water and reagents are used instead of the sample. And a dispensing means for dispensing the sample and the reagent into the remaining reaction container and the moving path of each reaction container, which is generated by the sample and the reagent in each reaction container for a predetermined absorption wavelength. Measuring means for respectively measuring the absorbance and the change in the absorbance of the mixed solution consisting of the same amount of distilled water and the reagent instead of the reaction solution and the sample, the storage means for storing the output signal output from the measuring means, and the storage means for outputting the output signal. Calculating a difference and an average value of the nth and (n + 1) th mixed solution measurement signals output before and after each reaction solution measurement based on the stored signal, and comparing the difference with a predetermined allowable value, The difference is When it is smaller than the value, the average display value or the n-th mixed solution measurement signal is subtracted from each of the reaction solution measurement signals of the n-th and subsequent ones to calculate the concentration of the test component in the sample, and the operation of each means. It is an automatic analyzer comprising control means for controlling.

(E) Embodiments The present invention will be described in detail based on the embodiments shown in the drawings. The present invention is not limited to this.

In Fig. 1, the automatic analyzer (1) has a large number of reaction vessels.
Transport means (2) for transporting (3) along a predetermined route, and a portion for dispensing a fixed amount of sample (6) and reagent (7) on the erection table (5) into each reaction container (3). Mixture consisting of the injection means (4) and the same amount of distilled water and reagent in each reaction vessel (3), that is, the reagent blank solution and the absorbance of the reaction solution produced by the sample (6) and reagent (7) And a measuring means (8) for measuring the change in absorbance,
Based on the detection signal from the measuring means (8), the average value of the absorbance of the reagent blank solution measured before and after the measurement of each reaction solution and the concentration of the test component are calculated and displayed, and after each reaction solution measurement It is composed of an analysis processing unit (9) for storing the measured absorbance of the reagent blank solution, and a control unit (10) for controlling each of these units and each unit.

As the transfer means (2), an endless transfer conveyor that is intermittently moved is usually used.
The rotary table may be used to allow intermittent movement. As the dispensing means (4) for the sample (6), the sample pipette (1
1) is used, and as the dispensing means (4) for the reagent (7), the same number of reagent dispensers (12) as the type of the reagent (7) used is used. (13) is a pure water well created so that pure water will flow out.This pure water well (13) is used not only for washing the sample pipettor (11) but also for reagent blanks. It is also used to separate pure water.

A multi-wavelength photometer (14) is used as the measuring means (8). This multi-wavelength photometer (14) consists of a light source (15) and a flow cell (1
6), a diffraction grating (17), and a plurality of detectors (18), and a reagent blank solution and a reaction solution in each reaction container (3) that has been transported by the transport means (2), Sequentially
The transmitted light from the light source (15), which is moved into the flow cell (16) and is irradiated to these liquids in the flow cell (16), is reflected by the diffraction grating (17) and detected by a plurality of detectors (18). To be done. The analysis processing unit (9) includes a multiplexer (19), an absorbance conversion unit (20), an A / D conversion unit (21), a data storage unit (22) including a microcomputer, and an operation unit (2) as operation display means.
3) and a data output display section (24). The multiplexer (19) is connected to each detector (18) of the measuring means (8) and transmits the detection signal received from the detector (18) to the absorbance converter (20). In the absorbance converter (20), the input detection signal is converted to absorbance, and the reagent blank value and the concentration of the test component of the reaction solution, which are repeatedly measured in the order of the reagent blank solution and the reaction solution, are A / D converters (21). Through the data storage section (2
It is stored in 2). The calculation unit (23) exudes the concentration of the test component, and in this calculation unit (23), the average value of the reagent blank values measured before and after that is subtracted from the measured value of each reaction solution, and the remaining value Is calculated by multiplying the concentration conversion coefficient to the concentration of the test component, and the result is displayed on the display unit (24).
Printed by. When the reagent blank value becomes stable at an allowable constant value, the subsequent measurement of the reagent blank value can be omitted and the analysis can be continued using the stable reagent blank value.

Next, the operation of the device will be described.

When an instruction to start analysis is given to the automatic analyzer (1), the sample pipettor (11) collects the same amount of distilled water from the pure water well (13) as the sample (6) according to the test substance and the reaction container. Collect in (3).
Then, the reagent for analyzing the component to be tested (7) is dispensed into the same reaction container (3) by the reagent dispenser (12) in a fixed amount. In this embodiment, the case where two kinds of reagents (7) are used is shown, but the kinds of reagents (7) are not limited to two kinds and may be increased or decreased as necessary. When the preparation of the reagent blank solution is completed, the conveying means (2) is driven and the next reaction container (3) is locked at a predetermined position. In this reaction container (3), a fixed amount of sample (6) is sampled from the sample container on the erection table (5), and the reagent (7) reacts with a fixed amount as in the case of preparation of the reagent blank solution. The reaction solution is prepared by dispensing into the container (3). A reagent blank solution is prepared again in the reaction container (3) next to this reaction solution.

Each reaction vessel (3) in which reagent blank solution and reaction solution were prepared
Is transported to the multi-wavelength photometer (14) by the transport means (2). The value of each absorbance measured here is stored in the data storage unit (22), the average value of the reagent blank solution measured before and after it is subtracted from the measured value of the reaction solution, and the calculation by multiplying the concentration conversion coefficient is performed. The calculation (23) is performed to obtain the concentration of the test component, and the data output display (24) prints it.

When there are two or more samples, the reagent blank solution and the reaction solution are alternately and repeatedly prepared, and one more reagent blank solution than the number of samples is prepared, and each reaction solution is used as the reagent blank solution before and after it. In combination, the concentration of the test component in each sample can be determined in the same manner as described above. In this case, when the reagent blank value measured repeatedly becomes stable at an acceptable constant value, the measurement of the reagent blank value thereafter may be omitted and the average value of the last two reagent blank values may be shared. it can.

In general, the concentration Cs-n of the test component in the n-th reaction solution is calculated by the following formula (see FIG. 3).

Where K: Concentration conversion factor of test component As-n: Measured value of nth reaction solution RB-n: Measured value of nth reagent blank solution RB- (n + 1): (n + 1) th reagent Measured value of blank solution In addition, p
Then, when (RB-n)-[RB- (n + 1)] <P, the concentration Cs- (n + 1) of the test component in the (n + 1) th reaction solution is
Is calculated by the following equation (see FIG. 3).

Fig. 2 shows an example of changes in the number of measurements and absorbance of the reagent blank solution after the routine is completed or during nighttime analysis of 24 hours standby, and Fig. 3 uses the reagent blank solution showing the absorbance changes of Fig. 2. It can be seen from the example of the measured absorbance value of the reaction solution that the absorbance of the reagent in the reaction solution is correctly estimated.

In addition, in the above-described embodiment, the discrete batch processing type automatic analyzer using the flow cell is described, but it is needless to say that the same can be applied to the reaction vessel direct photometric type automatic analyzer.

(F) Effect The present invention alternately prepares a mixed solution of distilled water and a reagent and a reaction solution in a large number of reaction vessels transported by a transporting means,
From the measured value of the reaction solution, before and after that, the average value of the absorbance of the mixed solution measured at (n) and (n + 1) th or the absorbance of the nth mixed solution was subtracted as the reagent blank value, and the test component in the sample Since the concentration is determined, it is possible to correctly estimate the absorbance of the reagent in the reaction solution and reduce the number of prepared reagent solutions even when the reagent blank value varies. Therefore, the utilization rate of expensive reagents can be increased, the time required to prepare the reagent solution can be shortened, and the test components in the sample can be analyzed economically and efficiently.

[Brief description of drawings]

FIG. 1 is a block diagram showing an embodiment of the present invention, FIG. 2 is a diagram showing an example of changes in absorbance of a reagent blank solution, and FIG. 3 is an example of analysis using the reagent blank solution. (1) …… Automatic analyzer, (2) …… Transport means, (3) …… Reaction container, (4) …… Dispensing means, (6) …… Sample, (7) …… Reagent,
(8) …… Measuring means, (9) …… Analysis processing section, (10) …… Control section, (22) …… Storage section, (23) (24) …… Calculation section, data output display section (calculation Display means).

Claims (2)

[Claims] 1. An equal amount of distilled water and a reagent instead of a sample are dispensed to every other one of a large number of reaction vessels which are sequentially transported by a transport means, and the sample and the reagent are dispensed to the remaining reaction vessels. Dispensing means for pouring, provided in the moving path of each reaction container, for the predetermined absorption wavelength, the reaction liquid generated by the sample and reagent in each reaction container, and a mixed liquid consisting of the same amount of distilled water and reagent instead of the sample Measuring means for respectively measuring the absorbance and the change in the absorbance, a storage means for storing the output signal output from the measuring means, and two output before and after each reaction solution measurement based on the storage signal output from the storage means. Calculation display means for calculating an average value of the mixed solution measurement signal and subtracting the average from each reaction solution measurement signal output during the mixed solution measurement to calculate / display the concentration of the test component in the sample, and Each hand Automatic analyzer comprising a control means for controlling the operation. 2. An equal amount of distilled water and a reagent instead of the sample are dispensed to every other one of a large number of reaction vessels that are sequentially arranged and transported by the transporting means, and the sample and the reagent are placed in the remaining reaction vessel rows. Dispensing means for dispensing, provided in the moving path of each reaction container, mixing the reaction liquid generated by the sample and reagent in each reaction container for the predetermined absorption wavelength and the same amount of distilled water and reagent replacing the sample Measuring means for measuring the absorbance and change in absorbance of the liquid, storage means for storing the output signal output from the measuring means, and output before and after each reaction solution measurement based on the storage signal output from the storage means ( n
+1) The difference and average value of the mixed solution measurement signal are calculated, and the difference is compared with a predetermined allowable value. When the difference is smaller than the allowable value, the average value or the n-th mixed solution measurement is performed. An automatic analyzer comprising a calculation display means for calculating the concentration of a test component in a sample by subtracting a signal from each of the reaction solution measurement signals of the nth and subsequent ones, and a control means for controlling the operation of each means.