JPH0621865B2 - Method for measuring optical properties of sample - Google Patents

Description

Description: TECHNICAL FIELD The present invention relates to a measuring method for measuring optical characteristics of a sample such as serum to be analyzed in a biochemical analyzer, which changes due to chyle, jaundice, hemolysis and the like. It is a thing.

(Prior Art) When analyzing a sample such as serum with a biochemical analyzer, the analysis is performed in order to obtain an accurate measurement value by correcting the measurement error due to the milkiness, jaundice, hemolysis and other various interfering chromogens in the sample. The degree of their influence is measured for each item, and a correction value is calculated using this photometric output to obtain a correct analysis value.

In order to detect the degree of the influence of chyle, jaundice, hemolysis, etc. of a sample, for example, Japanese Patent Publication No. 54-63785 discloses that when analyzing an analysis item that does not cause absorbance of a target substance in the visible wavelength range, A technique for measuring the amount of interfering chromogen in a sample from the change in absorbance occurring in the range is disclosed. This method is superior to the known sample blank method in that there is no need to use a sample blank, but it detects the amount of interfering chromogen only in the case of an analysis item that uses detection that does not react in the visible wavelength range. In addition, it is not only necessary to dispense reagents used for measurement of items that do not need to be measured, such as when measuring only the degree of milkiness, etc. There is a problem that it is not possible to use the most suitable one for the measurement of.

Further, in JP-A-55-26454, as a method for measuring the sample permeability, a nozzle at the tip of a cylinder containing a liquid absorption plunger is dipped in the sample and the suction plunger is moved to move the sample to the cylinder. A method is disclosed in which the sample is sucked into a provided flat sample chamber and the transmittance of the sample is measured while the sample is accommodated in the flat sample chamber. However, since this method uses a flat sample chamber, it is not easy to completely suck and exhaust the sample. Therefore, not only is there a risk of contamination, but there is a problem that the sample that has been measured cannot be used for measurement for other analytical items.

(Object of the invention) The object of the present invention is to solve the problems in the conventional method of measuring the permeability of a sample as described above, and to improve the permeability of the sample to be biochemically analyzed by chyle, jaundice, hemolysis and the like. An object of the present invention is to provide a method for measuring optical characteristics of a sample, which can measure the effect efficiently and accurately without affecting the sample at all.

(Summary of the Invention) A method for measuring optical properties of a sample of the present invention is one in which a sample is collected by aspiration from a plurality of sample containers one by one, and these samples are respectively injected into a plurality of reaction containers. In the method for measuring an optical property of a sample, which is used for measuring a specific substance in a sample by measuring light having a specific wavelength, the optical property of the sample, which affects an analysis value, in the method for measuring an optical property of a sample, The step of temporarily stopping the transfer of the sample at a predetermined photometric position in the transfer system path for transferring the sample to the optical path, the step of measuring the sample stopped at the photometric position at a predetermined wavelength to obtain a photometric signal, and the photometric signal. Determining an optical specific value that affects the analysis value of the sample based on the signal, storing the optical characteristic value, restarting the sample transfer, and injecting the sample into the reaction container, Analyzed in the reaction vessel And a step of correcting an analytical value of the sample with the optical characteristic value.

(Example) FIG. 6 is a diagram showing a main part of a configuration of an example in which the method according to the present invention is applied to an automatic analyzer.

In the figure, 1 is a sample guide line of the analyzer, 2 is a reaction line, and along the sample guide line 1, a plurality of sample containers 3-1, 3-2, ...
The reaction vessels 4-1 and 4-2 are transferred in the direction of arrow B, and the sample vessels 3-1 and 3-2 are at the sampling position a,
The reaction vessels 4-1, 4-2, ... Are configured to be temporarily stopped at the injection position b.

Reference numeral 5 is a sampling tube having an arbitrary cross-sectional shape such as a circular cross section or a rectangular cross section formed of a colorless translucent material. In this embodiment, the sample dispensing nozzle of the automatic analyzer is also used as the sample collecting pipe 5 and the sample container 3-3 which is stopped at the sample collecting position a.
The sample inside is sampled by the sampling tube 5, and the injection position b
After the sample sampling tube 5 is temporarily stopped at the photometric position c in the transfer path for transferring to the stopped reaction container 4-3, and after the sample is discharged at the injection position b, it is washed at the cleaning position (not shown). After that, it returns to the sampling position a again, and is automatically controlled by a control device (not shown) so as to prepare for the next sampling.

Photometric position c in the transfer route of the sample by the sampling tube 5
Includes a light source lamp 6 and a rotary filter device 8 including a plurality of interference filters 7-1, 7-2 ... Having different transmission wavelength ranges.
The photometric unit 10 having a well-known configuration including the photoelectric conversion element 9 outputs from the photoelectric conversion element 9 an output corresponding to the optical characteristic of the sample in the sampling tube 5 temporarily stopped at the photometric position c, for example, the transmittance. It is arranged so that it can be obtained.

Reference numeral 11 is an arithmetic unit for calculating an accurate transmittance of the sample with respect to the measurement wavelength by using a detection signal corresponding to the transmittance of the sample with respect to the photometric wavelength by the well-known two-wavelength method obtained by the photometry unit 10. That is, when the method according to the present invention is carried out in an analyzer as in this embodiment, the arithmetic unit 11 affects the measurement accuracy of each desired analysis item, for example, the degree of milkiness, jaundice, hemolysis, etc. It is also possible to obtain the amount and perform arithmetic processing on the correction value and the accurate measurement value of the correction result.

In addition, 12 is a washing / diluting liquid container containing the washing / diluting liquid 13, 14 is a syringe pump for feeding the washing / diluting liquid 13 to the sampling tube 5, 15 is a syringe pump for sucking and discharging the sample,
Reference numerals 16 and 17 denote first and second opening / closing valves, and 18 denotes a cleaning / diluting liquid supply pipe, and these pumps and opening / closing valves are automatically controlled by a controller (not shown) like the sampling pipe 5.

In the configuration of the above-described embodiment, the first opening / closing valve 16 is closed and the second opening / closing valve 16 is closed.
By opening the on-off valve and sucking the cleaning / diluting liquid by the syringe pump 14, closing the second on-off valve 17, opening the first on-off valve 15 and pressing the syringe pump 14, the syringe pump 14 is at a cleaning position (not shown). After the cleaning / diluting liquid is fed to the sampling pipe 5 for cleaning, the first opening / closing valve 16 is closed to fill the cleaning / diluting liquid including the inside of the feeding pipe 18 with the sampling pipe 5. 5
Is transferred to the illustrated sampling position a.

Then, the tip of the sampling tube 5 is immersed in the sample in the sample container 3-3 which is stopped at the sampling position a and is stopped at the position a, and a quantitative sample is sampled by the sample syringe pump 15. After aspirating and collecting, it moves up and transfers the sample to the photometric position c and pauses. The sample in the sample collection tube 5 is measured at the photometric position c by the photometric section 10 by two wavelengths, the wavelength light corresponding to the analysis item and the wavelength light in the vicinity thereof, as described above, and each output corresponding to the absorbance. Is obtained in a time-division manner and sent to the arithmetic unit 11.

After the photometry in this way, the sample sampling tube 5 stopped at the photometry position 10 is transferred to the injection position b on the reaction line 2 and the sample syringe pump 15 is pressed to inject the injection position b.
The sample in the sample collection tube 5 is discharged and injected into the reaction container 4-3 which is suspended. After completion of this injection, the sampling tube 5
Is transferred to a washing position (not shown), washed as described above, one dispensing operation is completed, and the next sample dispensing is awaited.

The photometric outputs of the two waves from the photometric unit 10 guided to the arithmetic unit 11 are all stored in a memory in the arithmetic unit 11 and used for a predetermined arithmetic operation. For example, a difference value of two-wave photometric output is calculated to obtain an accurate transmittance for light in a specific wavelength range, or a correction necessary to correct the measurement value of an analysis item using the light in the specific wavelength range from the transmittance. The value etc. can be calculated.

In the above-described embodiment, the photometric unit 10 is provided with the rotary filter device 8 between the light source lamp 6 and the sampling tube 5, converts the light from the light source lamp 6 into monochromatic light, irradiates the sample, and photoelectrically transmits the transmitted light. An example in which it is configured to lead to the conversion element 9 has been shown.
The photometric unit in the device of the present invention is not limited to that. For example, direct light from the light source lamp 6 is supplied to the sampling tube 5
It is also possible to irradiate a sample in the sample, guide the transmitted light from the sample to a rotary filter device, select a desired wavelength range and perform photoelectric conversion by the photoelectric conversion element 9, or rotation in the structure. A diffraction grating may be arranged instead of the filter device, and a plurality of photoelectric conversion elements may be simultaneously subjected to photoelectric conversion for each predetermined wavelength range to selectively extract photoelectric conversion output in a desired wavelength range.

Further, in the above-mentioned embodiment, the sample in the sample collecting tube 5 is all discharged to the reaction container after photometry, but it is not necessary to perform an analytical measurement, and only the transmittance of the sample for a predetermined wavelength can be measured. If it is good, the light may be returned to the original sample container as soon as the light measurement by the light measurement unit 10 is completed. That is, when the method according to the present invention is carried out in the analyzer as in the above-mentioned embodiment, the optical characteristics of the sample can be measured at the stage of transferring the sample from the sample container to the reaction container.
Therefore, since the sample remains in its original state, there is no problem even if it is returned to the original sample container, and the sample can be used for other measurement items without waste.

(Effect of the Invention) As described in detail above, according to the method of the present invention,
Since the transmittance of the sample is measured by the photometric section provided in the transfer path for transferring the sample to the reaction container, the photometry can be performed without any chemical operation on the sample. Therefore, in the conventional measurement method, when measuring a diluted sample, depending on the selection of the diluting solution, the milkiness and the like may change, but in the method according to the present invention,
Not only is there no need for a diluting solution or reagent solution, the running cost is reduced, and since the optical characteristics of the sample are measured by the dual wavelength method, the measurement accuracy is also improved. There are effects such as high.

In the analysis method for biochemical examination, the method according to the present invention is used as an optical characteristic method of a sample in the biochemical analysis method in order to measure the amount of interfering chromogens such as chyle, jaundice, and hemolysis that affect the analysis value. If the sample is not only able to contribute greatly to the improvement of analytical measurement accuracy, but it is not necessary to perform analytical measurement and only the optical characteristics need to be measured, the sample can be returned to the sample container after measurement. Since it can be used for other purposes, the waste of the sample can be eliminated.

[Brief description of drawings]

FIG. 1 is a diagram showing a main part of a configuration of an embodiment of a method according to the present invention applied to an automatic analyzer. 1 …… Sample guide line, 2 …… Reaction line 3-1 to 3−4 …… Sample container 4-1 to 4-4 …… Reaction container 5 …… Sample sampling tube, 6 …… Light source lamp 7-1, 7−2 …… Interference filter 8 …… Rotary filter device 9 …… Photoelectric conversion element, 10 …… Photometry unit 11 …… Computing device, 12 …… Washing / diluting liquid container 13 …… Washing / diluting liquid, 14, 15 ...... Syringe pump 16, 17 …… Open / close valve, 18 …… Supply pipe.

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Takashi Tahara 2-43-2 Hatagaya, Shibuya-ku, Tokyo Inside Olympus Optical Co., Ltd. (56) Reference JP-A-57-108638 (JP, A) JP 56-21041 (JP, A) JP-A-55-26454 (JP, A)

Claims (1)

[Claims] 1. When a sample is sequentially sucked and sampled from a plurality of sample containers using a single sample collection tube and these samples are respectively injected into a plurality of reaction containers to analyze a specific substance in the sample, In a method for measuring an optical property of a sample, which measures an optical property of a sample that influences an analysis value with a light having a specific wavelength, the sample collection tube has a predetermined transfer path for transferring the sample to the reaction container. A step of temporarily stopping the transfer of the sample at the photometric position, a step of measuring the sample stopped at the photometric position at a predetermined wavelength to obtain a photometric signal, and an influence on the analysis value of the sample based on the photometric signal Obtaining an optical characteristic value to be given, storing the optical characteristic value, restarting the transfer of the sample, injecting this sample into the reaction container, and the analysis value of the sample analyzed in the reaction container. Correct with the optical characteristic values Optical characteristics measuring method of a sample, characterized by a step.