JPH0628599B2 - Multi-sample microbial activity measuring device - Google Patents
Multi-sample microbial activity measuring deviceInfo
- Publication number
- JPH0628599B2 JPH0628599B2 JP1211397A JP21139789A JPH0628599B2 JP H0628599 B2 JPH0628599 B2 JP H0628599B2 JP 1211397 A JP1211397 A JP 1211397A JP 21139789 A JP21139789 A JP 21139789A JP H0628599 B2 JPH0628599 B2 JP H0628599B2
- Authority
- JP
- Japan
- Prior art keywords
- sample
- container
- cell unit
- constant temperature
- heat
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 230000000813 microbial effect Effects 0.000 title claims description 13
- 238000005259 measurement Methods 0.000 claims description 23
- 239000004020 conductor Substances 0.000 claims description 4
- 239000011810 insulating material Substances 0.000 claims description 3
- 238000007707 calorimetry Methods 0.000 claims 2
- 238000004861 thermometry Methods 0.000 claims 1
- 239000000523 sample Substances 0.000 description 33
- 244000005700 microbiome Species 0.000 description 5
- 238000001514 detection method Methods 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- 230000002503 metabolic effect Effects 0.000 description 4
- 239000002689 soil Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 230000001066 destructive effect Effects 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000006241 metabolic reaction Methods 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 238000005192 partition Methods 0.000 description 2
- 239000013558 reference substance Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229940057995 liquid paraffin Drugs 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 239000013074 reference sample Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
Landscapes
- Investigating Or Analyzing Materials Using Thermal Means (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Description
【発明の詳細な説明】 産業上の利用分野 この発明は、カビ、バクテリヤなどの各種微生物および
それらを含む土壌、食品などにおける微生物の生化学的
代謝活動に伴う熱変化を検出する多試料生物活性測定装
置に関する。TECHNICAL FIELD The present invention relates to a multi-sample biological activity for detecting heat changes associated with biochemical metabolic activities of various microorganisms such as molds and bacteria and soils, foods, etc. containing them. Regarding measuring device.
従来の技術 薬剤作用の検定、食品の腐敗、土壌環境中の有機物の分
解性など、微生物活性の定量を必要とする系において、
実際にそれを計測しようとする場合、いろいろな条件が
要求される。この内、イ）薬剤濃度などの計測条件が広
範囲に選べるように多くの試料を同時に測定できるこ
と、ロ）液体状態だけでなく、固体あるいは固体と液体
が共存するような不均一な状態の試料においても測定で
きること、ハ）試料の状態を損なわずに非破壊的、非接
触的に測定できること、の３つの条件を同時に充足する
方法が未だ出現していない。Conventional technology In systems that require quantification of microbial activity, such as assay of drug action, food spoilage, degradability of organic matter in soil environment,
When actually trying to measure it, various conditions are required. Among these, a) many samples can be measured at the same time so that measurement conditions such as drug concentration can be selected over a wide range, and Has not yet appeared, and the method of simultaneously satisfying the three conditions of (3) that the measurement can be performed, and (3) non-destructive and non-contact measurement can be performed without impairing the condition of the sample.
そして従来このような微生物活性を測定する装置として
は、 (1)光学的検出装置、 (2)インピーダンスを計測す
る電気的装置、及び(3)微生物コロニー数を計測する寒
天培地培養装置等をあげることができる。And as a conventional device for measuring such microbial activity, there are (1) an optical detection device, (2) an electrical device for measuring impedance, and (3) an agar culture device for measuring the number of microbial colonies. be able to.
発明が解決しようとする課題 ところで、前記した(1)の光学的検出装置は、余り広い
範囲の微生物濃度で測定できないのに加えて、透明度の
高い液体試料にしか使用できず、(2)の電気的装置は、
電気的性質を検知できるきわめて限られた条件にしか使
用できず、さらに(3)の寒天培地培養装置は、一定時間
培養したときの静的な状態を観測しているにすぎず、培
養時間を変えて測定すると異った結果がでるというよう
な問題がある。DISCLOSURE OF THE INVENTION Problems to be Solved by the Invention By the way, the optical detection device of (1) described above can be used only for a highly transparent liquid sample in addition to being unable to measure a microorganism concentration in a too wide range, and (2) The electrical device is
It can be used only under extremely limited conditions where electrical properties can be detected.In addition, the agar culture device in (3) only observes a static condition after culturing for a certain period of time. There is a problem that different results will be obtained when changing and measuring.
そのうえこれらの装置(1)(2)(3)は、いずれも食品、土
壌などのような不均一な試料において、本来微生物が生
息する状態を保持したまま非破壊的に計測するのは不可
能であるという問題があった。In addition, these devices (1) (2) (3) are not capable of non-destructive measurement of non-uniform samples such as food, soil, etc. while maintaining the condition where microorganisms originally live. There was a problem that was.
そこでこの発明の発明者は、このような従来の測定装置
のもつ問題を解決するのに、代謝反応に基く熱変化（代
謝熱）の検出を原理として採用することを思いたち、そ
れに基いてこの発明を完成した。Therefore, the inventor of the present invention has thought to adopt the detection of heat change (metabolic heat) based on metabolic reaction as a principle in order to solve the problem of such a conventional measuring device, and based on that, Completed the invention.
そもそも代謝熱は細胞内の生化学的反応におけるエンタ
ルピー変化に対応するものであり、細胞代謝活性に完全
に比例し、したがって、微生物活性をもっとも忠実に反
映しており、計測できる微生物濃度範囲がきわめて広
い。また「熱」はあらゆる物理量のなかでもっとも透過
性が高く、どのような媒体をも通過することができるた
め、試料の状態を損なわずに、非接触的に検出すること
が可能であり、完全に非破壊的立場での計測が可能であ
る。このことは、液体状態の試料はもとより土壌や食品
など、試料の状態や形状による測定の制限が全くないこ
とを意味している。さらに、検出がアナログ値として得
られ、しかも経時変化の測定が可能なため、計算機によ
る高度な解析に向いている。In the first place, metabolic fever corresponds to enthalpy changes in intracellular biochemical reactions, and is completely proportional to cell metabolic activity, and therefore reflects the microbial activity most faithfully, and the measurable microbial concentration range is extremely high. wide. In addition, “heat” has the highest permeability among all physical quantities and can pass through any medium, so it is possible to detect in a non-contact manner without impairing the state of the sample, and It is possible to measure in a non-destructive position. This means that there is no limitation on the measurement depending on the state or shape of the sample, such as soil or food, as well as the sample in the liquid state. Furthermore, since the detection can be obtained as an analog value and the change over time can be measured, it is suitable for advanced analysis by a computer.
そこでこの発明の目的は、前記のような原理を採用して
従来の各種の測定装置のもつ前記のような問題を解決
し、試料の固体差によるばらつきを軽減し、測定の効率
を大幅に向上させることのできる測定装置を提供するこ
とである。Therefore, an object of the present invention is to solve the above-mentioned problems of various conventional measuring devices by adopting the above-described principle, reduce variations due to individual differences of samples, and significantly improve measurement efficiency. It is to provide a measuring device capable of performing the measurement.
課題を解決するための手段 この発明は、上記の目的を達成するために、断熱材製箱
体と蓋体とを有する装置本体内に高熱伝導性材からなる
恒温槽を配置し、この恒温槽の中に高熱伝導性材からな
るブロックで構成されている恒温容器を配置し、この恒
温容器のほぼ中心に対照熱測定セルユニットを、さらに
この対照熱測定セルユニットを包囲する位置に、複数の
試料熱測定セルユニットをそれぞれ穿設し、これらの対
照熱測定セルユニット及び試料熱測定セルユニットの内
底部には、下端に熱電対を具えた試料皿を配設し、これ
らの試料皿に試料容器が載置され、前記熱電対は下端面
が恒温容器によりその温度に維持され、上端面が試料容
器内の温度変化を捉えるようになっていることを特徴と
するものである。Means for Solving the Problems In order to achieve the above object, the present invention arranges a thermostatic bath made of a high thermal conductive material in an apparatus main body having a box made of a heat insulating material and a lid, and the thermostatic bath A constant temperature container composed of a block made of a high thermal conductive material is placed in the control constant temperature measuring cell unit at approximately the center of the constant temperature container, and a position surrounding the reference heat measuring cell unit is a plurality of units. A sample heat measuring cell unit is drilled, and a sample pan with a thermocouple at the lower end is arranged at the inner bottom of these control heat measuring cell unit and sample heat measuring cell unit. A container is placed, the thermocouple has a lower end surface maintained at the temperature by a constant temperature container, and an upper end surface catches a temperature change in the sample container.
作 用 前記のようなこの発明の多量試料微生物活性測定装置に
おいて、微生物細胞を含む液体、気体、固体及びこれら
の混合物よりなる試料は、それぞれ異る熱測定セルユニ
ット内に配置されて、相互に熱的な影響を受けることな
く、同時にその熱変化が検出され、それによって前記原
理のもとに微生物の活性が測定される。Operation In the above-described apparatus for measuring microbial activity of a large amount of samples of the present invention, samples composed of a liquid containing a microbial cell, a gas, a solid, and a mixture thereof are arranged in different thermal measurement cell units and mutually At the same time, the thermal change is detected without being thermally influenced, whereby the activity of the microorganism is measured according to the above principle.
実施例 この発明を図面に示す実施例を参照しながら説明する。Embodiments The present invention will be described with reference to the embodiments shown in the drawings.
第１図においてこの発明の実施例は、平面上に１個の対
象熱測定セルユニットならびに16個の試料熱測定セルユ
ニットの計17個の熱測定セルユニットを配置した恒温容
器11を有するものとなっている。ここで１はこの発明に
関わる多試料微生物活性測定装置の本体であって、本体
１は外蓋２を有する箱体３を具え、これらの外蓋２およ
び箱体３は断熱材による保護処理が施されている。箱体
３の内部には内蓋４を有する熱伝導性の高い導製等の恒
温槽５が収容され、この恒温槽５の外周に導製等のパイ
プ６がスパイラル状に巻かれ、さらにパイプ６を被覆す
るように熱伝導セメント層７が形成されている。パイプ
６はその一端が図示しない恒温水循環槽の供給パイプ８
に接続され、他端が返送パイプ９に接続され、パイプ６
内に恒温水を循環させることにより恒温槽５を所定温度
に維持するようにしている。10は中間蓋である。In FIG. 1, the embodiment of the present invention has a constant temperature container 11 in which a total of 17 heat measuring cell units, one target heat measuring cell unit and 16 sample heat measuring cell units, are arranged on a plane. Has become. Here, 1 is a main body of the multi-sample microbial activity measuring apparatus according to the present invention, the main body 1 comprises a box 3 having an outer lid 2, and these outer lid 2 and box 3 are protected by a heat insulating material. It has been subjected. Inside the box body 3, a constant temperature bath 5 of high conductivity having an inner lid 4 is housed, and a pipe 6 of high conductivity is wound around the outer periphery of the constant temperature bath 5 in a spiral shape. A heat conductive cement layer 7 is formed so as to cover 6. One end of the pipe 6 is a supply pipe 8 of a constant temperature water circulating tank (not shown).
To the return pipe 9 and the other end to the pipe 6.
By circulating constant temperature water inside, the constant temperature bath 5 is maintained at a predetermined temperature. 10 is an intermediate lid.
恒温容器11は恒温槽５内に収容されて、この恒温槽５に
よって所定温度に維持されるようになっており、この恒
温容器１１は熱伝導性が高いアルミニウム等のブロック
で構成されている。恒温容器11には第２図に示すように
平面でみてほぼ中心位置に対照熱測定セルユニットＲが
穿設され、さらに対照熱測定セルユニットＲを包囲する
位置に、相互の最近接間隔が５mmもしくはそれ以上の距
離であるように複数（この例では16個）の試料熱測定セ
ルユニッＳ１〜Ｓ16が穿設されている。対照熱測定セル
ユニットＲを含む全ての熱測定セルユニットは、最近接
の３個のセルユニットの中心が正三角形の頂点もしくは
できるだけそれに近い位置になるよう配置され、したが
って、セル間の隔壁が最小５mmであり、なおかつ一定平
面で最密充填になる構造をとっている。12はセルユニッ
トの蓋である。The constant temperature vessel 11 is housed in the constant temperature vessel 5 and is maintained at a predetermined temperature by the constant temperature vessel 5, and the constant temperature vessel 11 is made of a block such as aluminum having high heat conductivity. As shown in FIG. 2, the thermostat container 11 is provided with a reference thermal measurement cell unit R at a substantially central position as seen in a plane, and at a position surrounding the reference thermal measurement cell unit R, the closest distance to each other is 5 mm. Alternatively, a plurality (16 in this example) of sample thermal measurement cell units S1 to S16 are provided so that the distance is more than that. All the thermometric cell units including the reference thermometric cell unit R are arranged so that the centers of the three closest cell units are located at the vertices of an equilateral triangle or at positions as close as possible to each other, so that the partition walls between the cells are minimized. It is 5 mm and has a structure in which it is the closest packing in a certain plane. 12 is a lid of the cell unit.
試料熱測定セルユニットＳ１〜Ｓ16および対照熱測定セ
ルユニットＲの内底部には、下端に熱電対13を具えた試
料皿14が配設され、各熱電対13は下端面が恒温容器11に
よりその温度に維持され、上端面が試料皿14に載置され
た試料容器15内の温度変化を捉えるようになっている。At the inner bottom of the sample heat measuring cell units S1 to S16 and the control heat measuring cell unit R, a sample dish 14 having a thermocouple 13 at the lower end is arranged, and the lower end surface of each thermocouple 13 is controlled by a thermostatic container 11. The temperature is maintained, and the upper end surface catches the temperature change in the sample container 15 placed on the sample dish 14.
各熱電対13は上下端面の温度差にもとづく熱起電力に応
じた信号を直流増幅器16に送り、さらにマルチプレキサ
ー機能を有するアナログ・デジタル変換インターフェー
ス17を介してマイクロコンピューター18に取り込まれる
ようになっている。これらの信号は対照熱測定セルユニ
ットＲ内の熱電対の起電力と試料熱測定セルユニットＳ
１〜Ｓ16内の熱電対の起電力との差、すなわち各試料が
示す温度変化を、コンピューターにプログラムされた任
意の時間間隔でマルチプレキサーの働きにより順次フロ
ッピーディスク19に貯えられるとともにＣＲＴ画面20上
に表示されるようになっている。フロッピーディスク19
に貯えられたデーターは、あらかじめプログラムされた
計算式に従い、熱伝導により失われた熱量を補正して微
生物増殖に伴う真の熱生成量を得るとともに、それを増
殖関数にあてはめ、増殖速度定数や薬剤作用定数などの
生化学合パラメーターを演算して求めるようになってい
る。Each thermocouple 13 sends a signal corresponding to the thermoelectromotive force based on the temperature difference between the upper and lower end surfaces to the DC amplifier 16, and is further taken into the microcomputer 18 via the analog / digital conversion interface 17 having a multiplexer function. ing. These signals are the electromotive force of the thermocouple in the reference thermometric cell unit R and the sample thermometric cell unit S.
The difference from the electromotive force of the thermocouple in 1 to S16, that is, the temperature change indicated by each sample, is sequentially stored in the floppy disk 19 by the function of the multiplexer at an arbitrary time interval programmed in the computer and on the CRT screen 20. Is displayed. Floppy disk 19
The data stored in was obtained by correcting the amount of heat lost by heat conduction according to a pre-programmed calculation formula to obtain the true amount of heat generated by microbial growth, and applying it to the growth function to determine the growth rate constant and Biochemical parameters such as drug action constants are calculated and obtained.
次に上記のものの作用を使用状態とともに説明する。Next, the operation of the above will be described together with the state of use.
パイプ内６に所定温度の恒温水を循環させ、恒温槽５を
その温度に維持し、熱伝導により恒温容器11全体をその
温度に維持させる。Constant temperature water at a predetermined temperature is circulated in the pipe 6 to maintain the constant temperature bath 5 at that temperature, and the constant temperature container 11 as a whole is maintained at that temperature by heat conduction.
測定試料を入れ、あらかじめ恒温容器11と同じ温度に維
持しておいた16個の試料容器15を試料熱測定セルユニッ
トＳ１〜Ｓ16内に挿入し、また、対照熱測定セルユニッ
トＲ内に、基準対照物質、例えば一定量の流動パラフィ
ンを入れた試料容器を挿入する。このようにして試料を
セットして、ある時間が経過すると、各試料中に含まれ
ている微生物細胞の増殖あるいは細胞内代謝反応により
微小な熱変化が起こり始め、その変化は試料熱測定セル
ユニットＳ１〜Ｓ16内における試料と、対照測定セルユ
ニットＲ内における基準物質との温度差としてフロッピ
ーディスク19に貯えられ、またＣＲＴ画面20上に表示さ
れる。16 sample containers 15 in which the sample to be measured was placed and previously maintained at the same temperature as the constant temperature container 11 were inserted into the sample heat measuring cell units S1 to S16, and the reference heat measuring cell unit R was filled with the reference sample. A sample container containing a reference substance, for example a certain amount of liquid paraffin, is inserted. After setting the samples in this way, and after a certain period of time, a minute thermal change begins to occur due to the growth of microbial cells contained in each sample or the intracellular metabolic reaction. The temperature difference between the sample in S1 to S16 and the reference substance in the control measurement cell unit R is stored in the floppy disk 19 and displayed on the CRT screen 20.
各試料熱測定セルユニットＳ１〜Ｓ16内の試料は、互い
に隣接するもの同志が熱変化に影響しあうが、厚みが最
低５mmの金属隔壁の存在によって、上の影響が排除され
ることが実験的に確められた。The samples in each of the sample thermal measurement cell units S1 to S16 are adjacent to each other and affect each other with respect to the thermal change, but it is experimental that the above influence is eliminated by the presence of the metal partition wall having a thickness of at least 5 mm. Was confirmed.
なお、上記実施例における恒温水循環系は空気恒温槽に
よっても置き換えられる。すなわち、箱体３そのものが
空気恒温槽を構成し、恒温容器11が恒温に保たれる構造
のものである。その場合、恒温槽５およびそれに付随す
るパイプ６等は不要となる。The constant temperature water circulation system in the above embodiment may be replaced by an air constant temperature bath. That is, the box body 3 itself constitutes an air thermostatic chamber, and the thermostat container 11 is kept at a constant temperature. In that case, the constant temperature bath 5 and the pipes 6 and the like accompanying it are unnecessary.
また、熱測定セルユニットの数は上記実施例のように17
個に限ることはなく、18個あるいは特許請求の範囲の要
件を満たすそれ以上の複数個とすることができる。The number of thermal measurement cell units is 17 as in the above embodiment.
The number is not limited to 18 and may be 18 or more than that which satisfies the requirements of the claims.
第３図、第４図は恒温容器11の変形例を示す。第３図の
恒温容器11は平面上に36個の試料熱測定セルユニットを
配置したものである。また、第４図の恒温容器は平面に
18個の試料熱測定セルユニットを含む恒温容器11，1
1′，11″をさらに垂直方向に３段積み上げた構成で、
計54個の試料熱測定セルユニットを有するものである。3 and 4 show a modification of the constant temperature container 11. The constant temperature container 11 of FIG. 3 has 36 sample heat measuring cell units arranged on a plane. In addition, the thermostat container shown in Fig.
Constant temperature container 11,1 containing 18 sample heat measurement cell units
With a configuration in which 1 ', 11 "are further stacked in three layers in the vertical direction,
It has a total of 54 sample heat measurement cell units.
発明の効果 この発明では前記のようであって、恒温に維持された恒
温容器のなかに、生物細胞内代謝反応に伴う微小な熱変
化を計測するための熱測定セルユニットを複数個配置
し、これらの熱測定セルユニットの中に配置した微生物
細胞を含む試料の熱変化を相互に影響することなく同時
に検出するようになっており、隣接熱測定セルユニット
内の試料の熱変化が相互に影響されることなく測定セル
ユニットごとに正確に測定され、したがって各測定セル
ユニットごとの各異る試料につき一度にその熱変化を検
出することができて、短時間のうちに多くの試料微生物
の活性を正確に検出できるという効果がある。EFFECTS OF THE INVENTION In the present invention, as described above, in a thermostatic container maintained at a constant temperature, a plurality of thermometric cell units for measuring a minute thermal change accompanying a metabolic reaction in a living cell are arranged, It is designed to detect the thermal changes of samples containing microbial cells placed in these thermal measurement cell units at the same time without affecting each other, and the thermal changes of the samples in adjacent thermal measurement cell units affect each other. The measurement can be performed accurately for each measurement cell unit without being processed, and therefore its thermal change can be detected at once for each different sample of each measurement cell unit, and the activity of many sample microorganisms can be detected in a short time. Can be accurately detected.
第１図はこの発明の実施例の縦断面図、第２図は同上の
恒温容器の１例の平面図、第３図は同他の例の平面図、
第４図（Ａ）は同さらに他の例の平面図、（Ｂ）はその
縦断正面図である。 １……熱量計本体、３……箱体 ５……恒温槽、11……恒温容器 13……熱電対、14……試料皿 15……試料容器、18……マイクロコンピューターFIG. 1 is a vertical cross-sectional view of an embodiment of the present invention, FIG. 2 is a plan view of an example of the thermostat container, and FIG. 3 is a plan view of another example.
FIG. 4 (A) is a plan view of still another example, and FIG. 4 (B) is a vertical sectional front view thereof. 1 ... Calorimeter main unit, 3 ... Box 5 ... Constant temperature bath, 11 ... Constant temperature container 13 ... Thermocouple, 14 ... Sample dish 15 ... Sample container, 18 ... Microcomputer
Claims (1)
に高熱伝導性材からなる恒温槽を配置し、この恒温槽の
中に高熱伝導性材からなるブロックで構成されている恒
温容器を配置し、この恒温容器のほぼ中心に対照熱測定
セルユニットを、さらにこの対照熱測定セルユニットを
包囲する位置に、複数の試料熱測定セルユニットをそれ
ぞれ穿設し、これらの対照熱測定セルユニット及び試料
熱測定セルユニットの内底部には、下端に熱電対を具え
た試料皿を配設し、これらの試料皿に試料容器が載置さ
れ、前記熱電対は下端面が恒温容器によりその温度に維
持され、上端面が試料容器内の温度変化を捉えるように
なっていることを特徴とする多試料微生物活性測定装
置。1. A thermostatic bath made of a high thermal conductive material is arranged in an apparatus body having a box made of a heat insulating material and a lid, and a block made of the high thermal conductive material is arranged in the thermostatic bath. Place a thermostat container, place a control calorimetry cell unit in the approximate center of this thermostat container, and pierce a plurality of sample calorimetry cell units at positions surrounding the control thermometry cell unit. At the inner bottom of the measurement cell unit and the sample heat measurement cell unit, sample pans having thermocouples at the lower ends are arranged, sample containers are placed on these sample pans, and the thermocouples have a constant temperature container at the lower end surface. A multi-sample microbial activity measuring device, characterized in that the temperature is maintained at that temperature and the upper end surface catches the temperature change in the sample container.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1211397A JPH0628599B2 (en) | 1989-08-18 | 1989-08-18 | Multi-sample microbial activity measuring device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1211397A JPH0628599B2 (en) | 1989-08-18 | 1989-08-18 | Multi-sample microbial activity measuring device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0376570A JPH0376570A (en) | 1991-04-02 |
| JPH0628599B2 true JPH0628599B2 (en) | 1994-04-20 |
Family
ID=16605293
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1211397A Expired - Fee Related JPH0628599B2 (en) | 1989-08-18 | 1989-08-18 | Multi-sample microbial activity measuring device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0628599B2 (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001252066A (en) * | 2000-03-14 | 2001-09-18 | Daikin Ind Ltd | Bacteria count measurement method and device |
| JP4697434B2 (en) * | 2006-02-22 | 2011-06-08 | セイコーエプソン株式会社 | Carriage and recording apparatus including the carriage |
| JP4851831B2 (en) * | 2006-04-07 | 2012-01-11 | 学校法人明治大学 | Micro calorimetry apparatus and micro calorimetry method |
| JP5848020B2 (en) * | 2011-04-22 | 2016-01-27 | 特定非営利活動法人けいはんな文化学術協会 | Bioactivity measuring device |
| CN105319193A (en) * | 2015-11-13 | 2016-02-10 | 无锡艾科瑞思产品设计与研究有限公司 | Agricultural product microorganism activity detecting instrument |
| US20170191020A1 (en) * | 2015-12-30 | 2017-07-06 | Palo Alto Research Center Incorporated | Thermochromic sensing devices, systems, and methods |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL101424B1 (en) * | 1976-06-12 | 1978-12-30 | Politechnika Slaska Wincentego | MASS EXCHANGER WITH CELL FILLING |
-
1989
- 1989-08-18 JP JP1211397A patent/JPH0628599B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0376570A (en) | 1991-04-02 |
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